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Chinese Journal of Aeronautics 2023年第3期36卷 421-435页

作者： Yingxia ZHU miaomiao wan Wei CHEN Yun wanG Wenbin TU Fan XU School of Mechanical Engineering Jiangsu UniversityZhenjiang 212013China School of Mechanical Engineering Guilin University of Aerospace TechnologyGuilin 541004China

Two new size factors of cross-section hollow coefficient and bending degree are introduced to reveal the size effect of bending forming of bimetallic composite tube.Hollow coefficient and bending degree can limit the commonly used bent tube to the size description range of(0,2.00).The evolution laws of the cross-section distortion forms in the hollow coefficient-bending degree interval are revealed as well as the action of the mandrel-cores on the size effect.Results show the mandrel-cores filling can expand the forming limit of the bent tube,but also bring two other forming defects of wrinkle and rupture.The identification factor(hollow coefficient multiply bending degree)provides a method for querying the cross-section distortion forms of all composite bending tubes.In the identification factor interval(0,1.00),the distribution area of bending forming defects of the composite tube is continuous.The thin-walled composite bending tube collapses when identification factor in(0,0.39),wrinkles when identification factor in[0.39,0.50),and ruptures when identification factor in[0.50,1.00).The mathematical model of size effect is derived,by which the average cross-section distortion rate is found to distribute like a radial leaf in the hollow coefficient-bending degree qualified forming space.The best forming zone is hollow coefficient 0.46-0.68,and bending degree 0.25-0.47.

关键词： Bending Bimetallic composite tube Cross-section distortion Size factor Size effect Mandrel

网络化延续性康复护理对宫颈癌化疗患者希望水平及遵医行为的影响

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中国校医 2024年第2期38卷 130-133,137页

作者：张文茜王鑫万苗苗南阳市中心医院妇科一病区河南南阳473000

目的探讨网络化延续性康复护理在宫颈癌化疗患者中的应用价值。方法选取2020年9月—2022年9月某院收治的126例宫颈癌化疗患者,按入院顺序分为2组,各63例。对照组采取常规护理,观察组采取网络化延续性康复护理,2组均护理3个月。对比分析... 详细信息

目的探讨网络化延续性康复护理在宫颈癌化疗患者中的应用价值。方法选取2020年9月—2022年9月某院收治的126例宫颈癌化疗患者,按入院顺序分为2组,各63例。对照组采取常规护理,观察组采取网络化延续性康复护理,2组均护理3个月。对比分析2组希望水平、遵医行为、自我管理能力、负性情绪以及护理满意度。结果护理后,观察组Herth希望量表(HHI)中对现在与未来的积极态度评分为(10.01±0.79)分、采取的积极行动评分为(10.46±0.65)分、与他人保持亲密关系评分为(10.34±0.71)分及HHI总分为(30.81±5.02)分,相比于对照组的(8.96±0.81)分、(9.15±0.54)分、(9.02±0.69)分、(27.13±4.46)分,差异均有统计学意义(P均<0.001);观察组自我护理能力测定量表(ESCA)中自我护理技能评分为(35.46±6.01)分、健康认知评分为(53.97±6.74)分、自我概念评分为(22.63±4.10)分、自我责任感评分为(18.03±3.46)分及ESCA总分为(130.09±9.84)分,相比于对照组的(30.07±5.59)分、(45.83±6.02)分、(18.50±4.37)分、(15.94±3.15)分、(110.34±9.02)分,差异均有统计学意义(P均<0.001)。观察组焦虑评分为(43.20±4.76)分、抑郁评分为(44.59±5.06)分,相比于对照组的(46.92±4.19)分、(48.02±3.61)分,差异均有统计学意义(t=4.656、4.380,P均<0.001)。观察组遵医总依从率为96.83%、护理满意度为95.24%,相比于对照组的85.71%、84.13%,差异均有统计学意义(χ^(2)=4.881、4.203,P均<0.05)。结论网络化延续性康复护理能够减轻宫颈癌化疗患者负性情绪,提高希望水平,改善遵医行为,增强自我管理能力,且能够获得较高的护理满意度。

关键词：宫颈癌化学疗法康复护理希望自我管理

LettuceGDB:The community database for lettuce genetics and omics

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Plant Communications 2023年第1期4卷 87-99页

作者： Zhonglong Guo Bo Li Jianjun Du Fei Shen Yongxin Zhao Yang Deng Zheng Kuang Yihan Tao miaomiao wan Xianju Lu Dong wang Ying wang Yingyan Han Jianhua Wei Lei Li Xinyu Guo Chunjiang Zhao Xiaozeng Yang Beijing Academy of Agriculture and Forestry Sciences Beijing 100097P.R.China State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Life Sciences and School of Advanced Agricultural SciencesPeking UniversityBeijing 100871P.R.China Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology Beijing Agro-biotechnology Research CenterBeijing 100097P.R.China College of Biology and the Environment Nanjing Forestry UniversityNanjing 510275P.R.China Beijing Key Lab of Digital Plant Beijing Research Center for Information Technology in AgricultureBeijing 100097P.R.China WeiRan Biotech Beijing 100085P.R.China Beijing Key Laboratory of New Technology in Agricultural Application Beijing University of AgricultureBeijing 102206P.R.China

As a globally popular leafy vegetable and a representative plant of the Asteraceae family,lettuce has great economic and academic significance.In the last decade,high-throughput sequencing,phenotyping,and other multi-omics data in lettuce have accumulated on a large scale,thus increasing the demand for an integrative lettuce database.Here,we report the establishment of a comprehensive lettuce database,LettuceGDB(https://***/).As an omics data hub,the current LettuceGDB includes two reference genomes with detailed annotations;re-sequencing data from over 1000 lettuce varieties;a collection of more than 1300 worldwide germplasms and millions of accompanying phenotypic records obtained with manual and cutting-edge phenomics technologies;re-analyses of 256 RNA sequencing datasets;a complete miRNAome;extensive metabolite information for representative varieties and wild relatives;epigenetic data on the genome-wide chromatin accessibility landscape;and various lettuce research papers published in the last decade.Five hierarchically accessible functions(Genome,Genotype,Germplasm,Phenotype,and O-Omics)have been developed with a user-friendly interface to enable convenient data access.Eight built-in tools(Assembly Converter,Search Gene,BLAST,JBrowse,Primer Design,Gene Annotation,Tissue Expression,Literature,and Data)are available for data downloading and browsing,functional gene exploration,and experimental practice.A community forum is also available for information sharing,and a summary of current research progress on different aspects of lettuce is included.We believe that LettuceGDB can be a comprehensive functional database amenable to data mining and database-driven exploration,useful for both scientific research and lettuce breeding.

关键词： lettuce genome multi-omics germplasms breeding community

CRISPR/Cas9系统敲除山羊CDK2基因载体构建及转染效率检测

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中国草食动物科学 2024年第2期44卷 27-31页

作者：万苗苗阳倩陈吉璐陈丹丹毛林班谦陈胜惠文巧安徽大学生命科学学院干细胞及转化医学研究中心合肥230601 安徽省农业科学院畜牧兽医研究所畜禽产品安全工程安徽省重点实验室合肥230031

细胞周期蛋白依赖性蛋白激酶-2(CDK2)是细胞通过和进入S期所必须的细胞周期调节激酶,与许多癌细胞的生长有关。然而,目前在山羊上关于CDK2基因敲除及相关功能的研究尚未见报道。研究采用CRISPR/cas9系统,首先设计CDK2基因的sgRNA片段,... 详细信息

细胞周期蛋白依赖性蛋白激酶-2(CDK2)是细胞通过和进入S期所必须的细胞周期调节激酶,与许多癌细胞的生长有关。然而,目前在山羊上关于CDK2基因敲除及相关功能的研究尚未见报道。研究采用CRISPR/cas9系统,首先设计CDK2基因的sgRNA片段,在合成CDK2 sgRNA核苷酸序列后,构建能够同时表达sgRNA和Cas9 D10A的质粒PYSY-CDK2 sgRNA,连接并转化至大肠杆菌DH5α感受态细胞,对转化子进行验证,最后转染HEK293T细胞,检测细胞荧光和细胞增殖。酶切和测序结果证明,CDK2基因敲除载体构建正确;荧光显微镜检测显示,细胞转染效率在75%以上;细胞增殖检测表明,山羊CDK2敲除质粒不会对人源细胞增殖产生影响。说明采用CRISPR/Cas9构建的载体,可为后续获得山羊CDK2基因缺失型细胞系,研究支原体肺炎感染引发的细胞凋亡分子机制提供理论依据。

关键词： CRISPR/Cas9 山羊 CDK2 基因敲除

骨折联络服务对脆性骨折患者干预效果的系统评价

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老年医学研究 2024年第3期5卷 14-20页

作者：汪苗苗程溢芬段文静刘玉婷万青安徽中医药大学护理学院安徽合肥230038 安徽中医药大学第一附属医院骨伤二科安徽合肥230031

目的系统评价骨折联络服务(FLS)在脆性骨折患者中的应用效果。方法检索PubMed、Web of Sci-ence、中国知网、万方和维普数据库中2014年1月1日—2023年12月30日关于FLS对脆性骨折患者进行干预的相关文献。采用偏倚风险评估工具2.0和渥太... 详细信息

目的系统评价骨折联络服务(FLS)在脆性骨折患者中的应用效果。方法检索PubMed、Web of Sci-ence、中国知网、万方和维普数据库中2014年1月1日—2023年12月30日关于FLS对脆性骨折患者进行干预的相关文献。采用偏倚风险评估工具2.0和渥太华评价量表分别对随机对照研究和观察性研究进行偏倚风险评价和质量评价。最后,基于数据质量评估,使用RevMan5.4分析软件进行Meta分析。结果本研究共纳入24篇文献,总计18558例脆性骨折患者。Meta分析结果显示,FLS可以缓解脆性骨折患者的疼痛(SMD=-1.25,95%CI:-1.39~-1.12,P<0.05)、降低二次骨折发生率(RR=0.60,95%CI:0.48~0.75,P<0.05)、减少病死率(RR=0.86,95%CI:0.80~0.93,P<0.05)、提高骨质疏松确诊率(RR=2.24,95%CI:1.74~2.87,P<0.05)、改善生活质量(SMD=10.34,95%CI:7.26~13.42,P<0.05)。结论FLS有助缓解脆性骨折患者的疼痛,降低二次骨折风险,减少病死率,提高脆性骨折患者骨质疏松的确诊率,提升患者的整体生活质量。

关键词：骨折联络服务脆性骨折二次骨折疼痛骨质疏松

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Lithium nitrate regulated carbonate electrolytes for practical Li-metal batteries: Mechanisms, principles and strategies

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Journal of Energy Chemistry 2023年第2期77卷 581-600,I0015页

作者： Kun wang Wenbing Ni Liguang wang Lu Gan Jing Zhao Zhengwei wan Wei Jiang Waqar Ahmad miaomiao Tian Min Ling Jun Chen Chengdu Liang Zhejiang Provincial Key Laboratory of Advanced Chemical Engineering Manufacture Technology College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027ZhejiangChina Institute of Zhejiang University-Quzhou Quzhou 324000ZhejiangChina

Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead Li.Significantly,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based electrolytes.Thus,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical LMBs.Regrettably,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate electrolytes.Thence,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective route.Therefore,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction strategies.This review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.

关键词： Li-metal battery Carbonate electrolyte Lithium nitrate Stabilization mechanism Dissolution principle Introduction strategy

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平邑甜茶发根农杆菌介导转化体系构建与优化

果树学报 2024年第5期41卷 999-1008页

作者：周雪婷武凯凯崔蓝芳张坤玺白团辉史江莉焦健王苗苗刘昱赵玉洁万然郝鹏博郑先波河南农业大学园艺学院郑州450046 河南省园艺植物生物学国际联合实验室郑州450046

【目的】建立并优化平邑甜茶(Malus hupehensis Rehd.)幼苗简单、高效、快速的发根农杆菌转化体系。【方法】以不同苗龄的平邑甜茶幼苗为材料,使用携带GFP及GUS过表达质粒的发根农杆菌K599,通过注射法、活化菌液浸染及菌株涂抹方法侵染... 详细信息

【目的】建立并优化平邑甜茶(Malus hupehensis Rehd.)幼苗简单、高效、快速的发根农杆菌转化体系。【方法】以不同苗龄的平邑甜茶幼苗为材料,使用携带GFP及GUS过表达质粒的发根农杆菌K599,通过注射法、活化菌液浸染及菌株涂抹方法侵染平邑甜茶根颈部诱导毛状根,利用GFP荧光检测、GFP蛋白印迹检测、DNA检测和GUS染色方法进行转基因株系验证,同时对成功转化株系根、茎、叶组织的表达量进行分析,检测发根农杆菌在转化平邑甜茶株系中的迁移性。【结果】不同苗龄平邑甜茶幼苗诱导毛状根能力具有较大差异,其中三叶龄幼苗诱导毛状根率最高,为96%,五叶龄幼苗最高的毛状根诱导率为65%,以使用菌株涂抹方式转化植株的毛状根分布为最佳、毛状根诱导率为最高。发根农杆菌在成功转化株系中存在随机向地上部迁移的现象,并能够整合目的基因至叶片叶柄及部分主叶脉基因组中,但在培养30 d时无法通过蛋白印迹方式在蛋白水平上检测到蛋白信号。【结论】建立并优化了简单、高效的发根农杆菌介导的平邑甜茶转化体系,鉴定了发根农杆菌在转化株系中随机向地上部迁移规律,为发根农杆菌技术的进一步利用提供理论依据。

关键词：平邑甜茶发根农杆菌转化效率迁移性

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The kinase p38αfunctions in dendritic cells to regulate Th2-cell differentiation and allergic inflammation

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Cellular & Molecular Immunology 2022年第7期19卷 805-819页

作者： miaomiao Han Jingyu Ma Suidong Ouyang Yanyan wang Tingting Zheng Peishan Lu Zihan Zheng Weiheng Zhao Hongjin Li Yun Wu Baohua Zhang Ran Hu Kinya Otsu Xinguang Liu Ying wan Huabin Li Gonghua Huang ENT Institute and Department of Otorhinolaryngology Eye&ENT HospitalFudan University200031 ShanghaiChina Shanghai Institute of Immunology Shanghai Jiao Tong University School of Medicine200025 ShanghaiChina Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics Guangdong Medical University523808 DongguanChina Biomedical Analysis Center Army Medical University400038 ChongqingChina Department of Cardiovascular Medicine Graduate School of MedicineOsaka UniversityOsaka 565-0871Japan School of Cardiovascular Medicine and Sciences King’s College LondonLondon SE59NUUK Present address:Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology430030 WuhanChina Department of Dermatology Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese Medicine200437 ShanghaiChina Department of Clinical Laboratory Medicine Shanghai Tenth People’s Hospital of Tongji University200072 ShanghaiChina Present address:Basic Department of Cancer Center Shanghai Tenth People’s Hospital of Tongji University200072 ShanghaiChina

Dendritic cells(DCs)play a critical role in controlling T helper 2(Th2)cell-dependent diseases,but the signaling mechanism that triggers this function is not fully understood.We showed that p38αactivity in DCs was decreased upon HDM stimulation and dynamically regulated by both extrinsic signals and Th2-instructive cytokines.p38α-specific deletion in cDC1s but not in cDC2s or macrophages promoted Th2 responses under HDM stimulation.Further study showed that p38αin cDC1s regulated Th2-cell differentiation by modulating the MK2−c-FOS−IL-12 axis.Importantly,crosstalk between p38α-dependent DCs and Th2 cells occurred during the sensitization phase,not the effector phase,and was conserved between mice and humans.Our results identify p38αsignaling as a central pathway in DCs that integrates allergic and parasitic instructive signals with Th2-instructive cytokines from the microenvironment to regulate Th2-cell differentiation and function,and this finding may offer a novel strategy for the treatment of allergic diseases and parasitic infection.

关键词： Allergy p38α dendritic cell Th2

软土地区施工现场重载车辆对基坑周围环境振动实测分析

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华南地震 2024年第2期44卷 128-135页

作者：万颖君金鑫马光辉张振宇翟洪刚汤方程孙苗苗杭州交通投资建设管理集团有限公司杭州310000 杭州市交通投资集团有限公司杭州310000 浙大城市学院土木工程系杭州310015 城市基础设施智能化浙江省工程研究中心杭州310015

以杭州某软土基坑为背景,通过在基坑周边地表布置加速度传感器,对重载车辆行驶引起的软土地区表层土体振动进行测试,得到软土地区临时施工道路的振动响应。通过统计各测点的加速度平均峰值、加速度振级以及振动主频,分析了软弱土体的振... 详细信息

以杭州某软土基坑为背景,通过在基坑周边地表布置加速度传感器,对重载车辆行驶引起的软土地区表层土体振动进行测试,得到软土地区临时施工道路的振动响应。通过统计各测点的加速度平均峰值、加速度振级以及振动主频,分析了软弱土体的振动响应,获取不同车速、测点方位等对土体振动响应的影响,根据重载车辆荷载对土体振动加速度以及振级随着离振源距离变化趋势最终划分基坑周围受重载车辆行驶振动的影响范围,得到软土地区基坑安全施工的范围。通过本次对杭州某软土基坑周边振动测试有助于全面认识重载车辆行驶振动对土体振动的传播规律,并对软土地区的基坑工程设计、安全施工及防灾减灾等提供依据。

关键词：软土地区重载车辆荷载加速度振动级振动主频

Bromination of Isothianaphthene Derivatives towards the Application in Organic Electronics

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Chinese Journal of Chemistry 2013年第11期31卷 1391-1396页

作者： Guankui Long Xiangjian wan Peng Yun Jiaoyan Zhou Yongsheng Liu miaomiao Li Mingtao Zhang Yongsheng Chen Key Laboratory for Functional Polymer Materials and Center for Nanoscale Science and Technology Institute of Polymer Chemistry Nankai University Tianjin 300071 China Computational Center for Molecular Science College of Chemistry Nankai University Tianjin 300071 China

brominate dialkyl isothianaphthene-5,6-dicarboxylate 的不同途径被试试，目标分子和方面产品的单个晶体被获得。有 trans-ethene 的平面共聚物被准备了，它在电影与边在普通器官的溶剂和宽广吸收显示出好溶解度到 967 nm。

关键词：溴化硫茚衍生物应用电子二羧酸二甲酯有机溶剂副产物