检索结果-南通市图书馆

Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18

Food Science and Human Wellness 2024年第2期13卷 611-620页

作者： Xianxian jia miaomiao jia Xiang Gao Xiang Li Mengyuan Wang Shengqiang Du Rui Huang Xiaotong Li Jun Zhang Shujin Li Chunling Ma Yan Zhang Bin Cong Department of Pathogen Biology Institute of basic MedicineHebei Medical UniversityShijiazhuang 050017China Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical SciencesShijiazhuang 050017China Institute of Basic Medicine Hebei Medical UniversityShijiazhuang 050017China College of Forensic Medicine Hebei Key Laboratory of Forensic MedicineCollaborative Innovation Center of Forensic Medical Molecular IdentificationHebei Medical UniversityShijiazhuang 050017China Hebei Food Safety Key Laboratory Hebei Food Inspection and Research InstituteShijiazhuang 050227China

Human normal flora is a source of probiotics.The safety characteristics of a specific isolate determine its application in foods or drugs.The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of the isolates from normal human flora.In this work,we assessed the in vitro pH tolerance,bile tolerance,biogenic amine production,mucin utilization,and safety of in vivo administration to mice to evaluate general health,organ-body weight index,organ histopathological change,whether L.gasseri HMV18 can colonize in the gut or modulate the gut microbiota after oral administration.The results suggest that L.gasseri HMV18 can tolerate pH 3 for 2 h,3%bile for 3 h,biogenic amine negative,mucin usage negative,does not encode verified toxins,and cause no visible change in mice's organs.L.gasseri HMV18 might not colonize in mice's gut,but can significantly affect the structure of gut microbiota.A bibliographical survey suggested that there were as few as 8 opportunistic infection cases from 1984 to 2022 and that the possibility for L.gasseri to cause infection is relatively low.Therefore,this work provides a basis for the foods or drugs application of L.gasseri HMV18 and gives a map of experiments for the safety assessment of probiotics.

关键词： Probiotics Lactobacillus gasseri Safety assessment Gut microbiota

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of novel genes associated with atherosclerosis in Bama miniature pig

引用

Animal Models and Experimental Medicine 2024年第3期7卷 377-387页

作者： Dengfeng Ding Yuqiong Zhao Yunxiao jia miaomiao Niu Xuezhuang Li Xinou Zheng Hua Chen Laboratory Animal Center Chinese PLA General HospitalBeijingChina

Background:Atherosclerosis is a chronic cardiovascular disease of great concern.However,it is difficult to establish a direct connection between conventional small animal models and clinical practice.The pig's genome,physiology,and anatomy reflect human biology better than other laboratory animals,which is crucial for studying the pathogenesis of atherosclerosis.Methods:We used whole-genome sequencing data from nine Bama minipigs to perform a genome-wide linkage analysis,and further used bioinformatic tools to filter and identify underlying candidate genes.Candidate gene function prediction was performed using the online prediction tool STRING 12.0.Immunohistochemistry and immunofluorescence were used to detect the expression of proteins encoded by candidate genes.Results:We mapped differential single nucleotide polymorphisms(SNPs)to genes and obtained a total of 102 differential genes,then we used GO and KEGG pathway enrichment analysis to identify four candidate genes,including SLA-1,SLA-2,SLA-3,and TAP2.nsSNPs cause changes in the primary and tertiary structures of SLA-I and TAP2 proteins,the primary structures of these two proteins have undergone amino acid changes,and the tertiary structures also show slight changes.In addition,immunohistochemistry and immunofluorescence results showed that the expression changes of TAP2 protein in coronary arteries showed a trend of increasing from the middle layer to the inner layer.Conclusions:We have identified SLA-I and TAP2 as potential susceptibility genes of atherosclerosis,highlighting the importance of antigen processing and immune response in atherogenesis.

关键词： atherosclerosis candidate genes genome-wide linkage analysis major histocompatibility complex whole genome sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NaOH–ball-milled co-modified magnetic biochar and its oil adsorption properties

引用

Particuology 2023年第12期83卷 40-49页

作者： Zehua Wei Haihong Li miaomiao jia Ting Lin School of Environmental and Chemical Engineering Xi'an Polytechnic UniversityXi'an710048China

A magnetic biochar adsorbent(BM–OH–BC)was prepared through NaOH–ball milling co-modification with walnut shells as the raw material,and its oil adsorption characteristics were investigated.The adsorption properties of oil pollutants were compared before and after the NaOH-ball-milling co-modification,and the mechanism of NaOH-ball-milling on oil pollutants was investigated by scanning electron microscope-energy dispersive X-ray spectroscopy,BET,X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy.The experimental results showed that the oil adsorption capacity of BM–OH–BC can reach 2.75 g/g,which is 129%higher than that of unmodified biochar.Moreover,after five adsorption and desorption cycles,the adsorption rate of BM–OH–BC only decreased by 5.9%,which was because the oil adsorption of BM–OH–BC is mainly chemical adsorption.The adsorption processes involving single layer,heterogeneous phase and multilayer made BM–OH–BC have excellent adsorption properties.After biochar co-modification,the specific surface area of BM–OH–BC increased to 466 m^(2)/g,the total pore volume increased to 0.245 cm^(3)/g,the average pore size decreased to1.90 nm,and the number of hydrophobic non-polar functional groups increased,leading to the enhancement of its oil absorption capacity.This study provides a reference for the treatment of oil-contaminated water bodies.

关键词： Biochar Oil pollution Adsorptionproperties Modificationmechanism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sub-5 nm porous polymer decoration toward superhydrophobic MOFs with enhanced stability and processability

引用

Chinese Chemical Letters 2022年第4期33卷 2134-2138页

作者： Siyi Rong Pengcheng Su Shizheng Chen miaomiao jia Wanbin Li Guangdong Key Laboratory of Environmental Pollution and Health School of EnvironmentJinan UniversityGuangzhou 511443China

Metal-organic frameworks(MOFs) show great potential for various applications, but many of them suffer from the drawbacks of hydrolysis propensity and poor processability. Herein, we employ polymers of intrinsic microporosity(PIMs) with hydrophobic pores to decorate MOFs toward substantially improved water stability and shapeability. Through simple PIM-1 decoration, the sub-5 nm polymer layers can be uniformly deposited on MOF surfaces with almost no deterioration in porosity. Owing to the existence of superhydrophobic coating and the obstruction of water entrance into MOFs, the PIM-1 coated Cu BTC exhibits impressive water resistance and excellent pore preservation ability after exposure in water, even in acidic and alkaline solutions. Moreover, polymer decoration improves the processability of MOFs, while various MOF/PIM-1 bulk wafers and oil-water separators can be obtained straightforwardly.

关键词： Metal-organic framework Porous polymer decoration Hydrophobicity Stability Processability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A new Lactobacillus gasseri strain HMV18 inhibits the growth of pathogenic bacteria

引用

Food Science and Human Wellness 2022年第2期11卷 247-254页

作者： Xiang Gao Zixuan Wang Xiang Li Xiaoling Zhang Shengqiang Du miaomiao jia Dailun Hu Xianxian jia Bin Cong Yan Zhang Chunling Ma Song Zhou Jun Zhang Department of Pathogen Biology Institute of Basic MedicineHebei Medical UniversityShijiazhuang 050017China Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical SciencesShijiazhuang 050017China Institute of Basic Medicine Hebei Medical UniversityShijiazhuang 050017China Shijiazhuang Great Wall Integrated Traditional Chinese and Western Medicine Hospital Shijiazhuang 050035China Hebei Food Safety Key Laboratory Hebei Food Inspection and Research InstituteShijiazhuang 050227China College of Forensic Medicine Hebei Key Laboratory of Forensic MedicineCollaborative Innovation Center of Forensic Medical Molecular Identifi cationHebei Medical UniversityShijiazhuang 050017China

To search for a new eco-friendly therapy for infectious disease caused by Escherichia coli,Staphylococcus aureus or Klebsiella oxytoca,we collected the vaginal swabs from healthy women,screened for Lactobacillus and found a strain repressing the growth of pathogenic bacteria.The new isolate was identified as L.gasseri by the colony morphology,Gram staining,biochemical reactions and confirmed by the 16 S rDNA sequencing.The HMV18 strain inhibited the growth of food-borne pathogens such as E.coli,S.aureus and K.oxytoca.The HMV18 strain was sensitive to penicillin,ampicillin,erythromycin,tetracycline and chloramphenicol.The HMV18 strain producedα-hemolysis.Pathological histology of the mice ileum showed that the mucosa,villi,lamina propria and crypt depth remained intact and there was no inflammation or hyperemia in the L.gasseri HMV18 gavaged group.L.gasseri HMV18 could not up-regulate inflammatory cytokines level of plasma.All the results suggested L.gasseri HMV18 is a candidate probiotic to be an additive for food preservation or drug to prevent food-borne diseases.

关键词： Lactobacillus gasseri Antagonism Bacteria Safety evaluation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

乡村振兴视域下乡村孝文化的传承发展研究——以濮阳市清丰县为例

引用

辽宁农业职业技术学院学报 2024年第1期26卷 25-29页

作者：贾苗苗河南理工大学河南焦作454000

乡村文化是中华优秀传统文化的有机组成部分,也是实现乡村振兴的重要思想资源,而孝文化是引领乡村文化回归本土特色不可或缺的重要元素,是加强社会治理、建设乡风文明的必要举措。以濮阳市清丰县为例,分析了当地孝文化的表征和发展所面... 详细信息

乡村文化是中华优秀传统文化的有机组成部分,也是实现乡村振兴的重要思想资源,而孝文化是引领乡村文化回归本土特色不可或缺的重要元素,是加强社会治理、建设乡风文明的必要举措。以濮阳市清丰县为例,分析了当地孝文化的表征和发展所面临的困境,提出了乡村振兴视域下乡村孝文化传承发展的新路径。

关键词：乡村振兴孝文化传承发展

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

收缩视角下黄河中游城市碳排放时空格局及其影响因素

引用

生态学报 2024年第3期44卷 903-914页

作者：郭亚丽贾俊松李玲胡淼淼吴钢江西师范大学地理与环境学院湿地与流域研究教育部重点实验室南昌330022 中国科学院生态环境研究中心城市与区域生态国家重点实验室北京100085

城市收缩作为城市化进程中的一种现象,与碳排放之间的关系错综复杂。当前从多维度收缩视角来探究碳排放量驱动因素的研究鲜有。据此,从人口、社会和经济三个维度构建城市收缩评价体系,利用黄河中游四省50个城市2012—2020年面板数据,基... 详细信息

城市收缩作为城市化进程中的一种现象,与碳排放之间的关系错综复杂。当前从多维度收缩视角来探究碳排放量驱动因素的研究鲜有。据此,从人口、社会和经济三个维度构建城市收缩评价体系,利用黄河中游四省50个城市2012—2020年面板数据,基于熵值法、夜间灯光指数及地理探测器等方法探究城市收缩对碳排放时空差异的影响。结果表明:①黄河中游资源型城市收缩现象最为严重,呈现“大分散,小聚集”特征。人口收缩占比最多,综合收缩最少。②碳排放总量在研究时段内从2327 Mt增长到3040 Mt,热点区域分布在鄂尔多斯市、乌海市、呼和浩特市、忻州市和朔州市;冷点区域从陕西省和河南省分散分布逐渐向河南省集聚。碳排放总量两极分化现象明显,高值区域数量少,数值大,且逐年增幅大;低值区域数量多,数值低,呈逐年降低趋势。成长型和成熟型资源城市是碳排放量的热点区域,同时城市收缩加剧了碳排放量;衰退型和再生型资源城市已经成为碳排放量的冷点区域,此时的城市收缩对碳排放量抑制作用加强。③城市收缩对于碳排放量的影响是复杂的,经济收缩对碳排放空间分异的解释力最强,人口收缩解释力最小,多数影响因素空间叠加后会产生双因子增强作用,且主导因素由人口和经济收缩转变为社会和经济收缩。可见,黄河中游城市碳排放时空格局及影响因素存在一定差异,因此,在制定双碳行动方案时应综合考虑这些差异,进而可提出有针对性且能适应于地区发展需要的节能减排对策。

关键词：城市收缩碳排放空间格局影响因素黄河中游城市

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

嗜线虫镰刀菌NQ8GⅡ4对枸杞根腐病的防效及其机制研究

引用

园艺学报 2024年第3期51卷 631-642页

作者：贾淑鑫李金闫思远郭苗苗王若彤顾沛雯宁夏大学农学院银川750021

为明确内生真菌嗜线虫镰刀菌(Fusarium nematophilum)NQ8GⅡ4菌株对枸杞根腐病的防效及作用机制,利用平板对峙法测定其对枸杞根腐病原菌(Fusarium oxysporum f. sp. lycium barbarum)菌丝生长的抑制作用,通过盆栽试验测定对枸杞根腐病... 详细信息

为明确内生真菌嗜线虫镰刀菌(Fusarium nematophilum)NQ8GⅡ4菌株对枸杞根腐病的防效及作用机制,利用平板对峙法测定其对枸杞根腐病原菌(Fusarium oxysporum f. sp. lycium barbarum)菌丝生长的抑制作用,通过盆栽试验测定对枸杞根腐病的防治效果,并采用防御酶活性测定和组织化学染色分析其作用机制。结果表明,预先3 d接种NQ8GⅡ4菌株能显著抑制尖孢镰刀菌菌丝的生长,抑制率高达70.07%。拌土法接种NQ8GⅡ4菌株可显著降低温室中枸杞根腐病的发生,防效为76.38%,与化学药剂多菌灵防效相当。接种NQ8GⅡ4菌株显著促进枸杞生长,株高、侧枝数、叶宽、茎粗和根长分别比对照增加了37.61%、90.56%、52.50%、32.35%和44.69%。在病原菌胁迫下,接种NQ8GⅡ4后21 d与不接种相比POD、SOD、PAL和CAT活性分别增加33.92%、65.64%、57.96%和41.59%,MDA含量减少44.23%。说明接种NQ8GⅡ4菌株可以促进枸杞幼苗生长,激发植株体内防御酶活性,缓解病原菌胁迫导致的膜脂过氧化作用,进而提高对根腐病的抗性,该菌株具有良好的生防潜力和应用前景。

关键词：枸杞根腐病内生真菌嗜线虫镰刀菌生物防治

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

大豆四种游离氨基酸高含量优异种质鉴定及GWAS分析

引用

植物遗传资源学报 2024年第6期25卷 957-966页

作者：熊雅文贾倩茹周苗苗张红梅陈华涛南京农业大学生命科学学院南京210095 江苏省农业科学院经济作物研究所南京210014 青岛农业大学农学院山东青岛266109

大豆籽粒中氨基酸含量丰富,是大豆品质的重要组成部分,具有较高的营养价值和生理功能。本研究利用高效液相色谱法测定264份大豆品种干籽粒中精氨酸、甘氨酸、谷氨酸、赖氨酸等4种游离氨基酸含量。结果表明,游离氨基酸中精氨酸含量最高... 详细信息

大豆籽粒中氨基酸含量丰富,是大豆品质的重要组成部分,具有较高的营养价值和生理功能。本研究利用高效液相色谱法测定264份大豆品种干籽粒中精氨酸、甘氨酸、谷氨酸、赖氨酸等4种游离氨基酸含量。结果表明,游离氨基酸中精氨酸含量最高、谷氨酸含量次之、甘氨酸含量最低,筛选出四种游离氨基酸均高含量的3个大豆优质品种,分别为海门羊104、辽鲜豆12号、灌云大四粒。结合大豆自然群体四种游离氨基酸含量和基因型进行全基因组关联分析,大豆四种氨基酸2年均可定位到的显著SNP位点共27个,其中精氨酸有2个SNP位点,包括S17_19067780和S17_19067789,甘氨酸有19个SNP位点,包括S01_53974257、S08_38878988等,谷氨酸有1个SNP位点,S18_53291599,赖氨酸有5个SNP位点,包括S08_18555689、S08_18567542等;并推测大豆氨基酸高含量相关候选基因,精氨酸为Glyma.17G177400和Glyma.17G177600,甘氨酸为Glyma. 11G157000和Glyma. 11G161700,谷氨酸为Glyma. 18G244200和Glyma. 18G244700,赖氨酸为Glyma. 08G227600和Glyma.08G228100。本研究结果为大豆品种改良、辅助大豆分子育种提供理论基础。

关键词：大豆游离氨基酸全基因组关联分析 SNP

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Whole-genome sequencing study to identify candidate markers indicating susceptibility to type 2 diabetes in Bama miniature pigs

引用

动物模型与实验医学(英文) 2023年第4期6卷 283-293页

作者： miaomiao Niu Yuqiong Zhao Yunxiao jia Lei Xiang Xin Dai Hua Chen Laboratory Animal Center Chinese PLA General HospitalBeijingPR China Beijing Institute of Orthopaedic Trauma Beijing Jishuitan HospitalBeijingPR China

Background:Hundreds of single-nucleotide polymorphism(SNP)sites have been found to be potential genetic markers of type 2 diabetes mellitus(T2DM).However,SNPs related to T2DM in minipigs have been less reported.This study aimed to screen the T2DM-susceptible candidate SNP loci in Bama minipigs so as to improve the success rate of the minipig T2DM model.Methods:The genomic DNAs of three Bama minipigs with T2DM,six sibling lowsusceptibility minipigs with T2DM,and three normal control minipigs were compared by whole-genome sequencing.The T2DM Bama minipig-specific loci were obtained,and their functions were annotated.Meanwhile,the Biomart software was used to perform homology alignment with T2DM-related loci obtained from the human genome-wide association study to screen candidate SNP markers for T2DM in Bama miniature pigs.Results:Whole-genome resequencing detected 6960 specific loci in the minipigs with T2DM,and 13 loci corresponding to 9 diabetes-related genes were selected.Further,a set of 122 specific loci in 69 orthologous genes of human T2DM candidate genes were obtained in the pigs.Collectively,a batch of T2DM-susceptible candidate SNP markers in Bama minipigs,covering 16 genes and 135 loci,was established.Conclusions:Whole-genome sequencing and comparative genomics analysis of the orthologous genes in pigs that corresponded to the human T2DM-related variant loci successfully screened out T2DM-susceptible candidate markers in Bama miniature pigs.Using these loci to predict the susceptibility of the pigs before constructing an animal model of T2DM may help to establish an ideal animal model.

关键词： Bama miniature pig candidate marker SNP type 2 diabetes whole-genome resequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：