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An ubiquitin-like protein SDE2 negatively affects sucrose-induced anthocyanin biosynthesis in Arabidopsis

Science Bulletin 2017年第23期62卷 1585-1592页

作者： Ye Xie Yulei Zhao Huijuan tan qin Chen Jirong Huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China University of the Chinese Academy of Sciences Beijing 100049 China School of Life Sciences Shanghai University Shanghai 200444 China College of Life and Environmental Sciences Shanghai Normal University Shanghai 200234 China

Anthocyanin biosynthesis is regulated by a conserved transcriptional MBW complex composed of MYB,b HLH and WD40 subunits. However, molecular mechanisms underlying transcriptional regulation of these MBW subunits remain largely elusive. In this study, we isolated an Arabidopsis mutant that displays a constitutive red color in aboveground tissues with retarded growth phenotypes. In the presence of sucrose, the mutant accumulates more than 3-fold anthocyanins of the wild type(WT), but cannot produce anthocyanins as WT in the absence of sucrose. Map-based cloning results demonstrated that the mutation occurs in the locus At4 G01000, which encodes a conserved nuclear-localized ubiquitin-like(UBL) superfamily protein, silencing defective 2(SDE2), in eukaryotes. SDE2 is ubiquitously expressed in various tissues. In the sucrose-induced anthocyanin biosynthesis, SDE2 expression was not responded to sucrose treatment at the early stage but was enhanced at the late stage. SDE2 mutations result in upregulation of anthocyanin biosynthetic and regulatory genes. Yeast-two hybrid analysis indicated that SDE2 has no direct interaction with the MYB transcription factor PAP1 and b HLH factor TT8, indicating that SDE2 is a indirect factor to affect anthocyanin accumulation. Taking together, our data suggest that SDE2 may play a role in finely coordinating anthocyanin biosynthesis with other biological processes.

关键词： Anthocyanin biosynthesis Sucrose SDE2 PAP1 Arabidopsis

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Effects of triclosan on gonadal differentiation and development in the frog Pelophylax nigromaculatus

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Journal of Environmental Sciences 2018年第2期30卷 157-165页

作者： Juan Chen tan Meng Yuanyuan Li Kun Gao Zhanfen qin State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China University of Chinese Academy of Sciences Beijing 100049 China

Previous studies have reported that triclosan（TCS） could possess an androgenic *** aimed to investigate the effects of TCS on gonadal differentiation and development in the frog Pelophylax nigromaculatus, a sensitive amphibian species to androgenic chemicals.P. nigromaculatus tadpoles at stage 24 were exposed to TCS（3, 30, and 300 nmol/L） to stage46 in a semi-static exposure system. At the end of exposure, gonadal morphology and histology, sex ratio and gonadal expression of sex-biased genes were examined in P. nigromaculatus. In each TCS treatment group, we found several individuals whose gonads exhibited morphological and/or histological abnormalities. Gonadal histological abnormalities were characterized by few oocytes and many somatic cells. Although the percentage of the individuals with abnormal gonads was low（7.8%） among all animals treated with TCS,statistical test revealed the sex ratios in the 3 and 300 nmol/L TCS treatment groups were significantly different from the solvent control. In the 30 nmol/L TCS treatment group,abnormal gonads were also observed, although the sex ratio was not changed compared with the solvent control, which was possibly due to the smaller sample size in this group. In all the TCS treatment groups, the sex ratios were not obviously male-biased, but the expression levels of some sex-biased genes were significantly altered by TCS. Altogether,our results suggest that TCS, even at environmentally relevant concentrations, could disrupt gonadal differentiation and development in P. nigromaculatus, but we are not sure whether the disrupting effects were associated with masculinization or feminization.

关键词： Triclosan Pelophylax nigromaculatus Gonadal abnormality Androgenic activity

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Better tillage selection before ridge–furrow film mulch can facilitate root proliferation, and increase nitrogen accumulation,translocation and grain yield of maize in a semiarid area

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Journal of Integrative Agriculture 2023年第6期22卷 1658-1670页

作者： ZHANG miao-miao DANG Peng-fei LIYu-ze qin Xiao-liang Kadambot H.M.SIDDIQUE College of Agronomy/Key Laboratory of Crop Physio-ecology and Tillage Science in Northwestern Loess Plateau Northwest A&F UniversityYangling 712100P.R.China The UWA Institute of Agriculture and School of Agriculture&Environment The University of Western AustraliaPerth WA 6001Australia

Plastic film mulch systems are used widely in arid areas, and the associated tillage measures affect soil properties, root and crop growth, and nutrient uptake. However, much debate surrounds the most suitable tillage method for plastic film mulch systems. We conducted a two-year field experiment to explore the impact of three tillage treatments-rotary tillage before ridge–furrow plastic film mulch(MR), no-tillage before ridge–furrow plastic film mulch(MZ), and plow tillage before ridge–furrow plastic film mulch(MP)-on soil total nitrogen, available nitrogen, root stratification structure,nitrogen transfer and utilization, and maize yield. The results showed that MP had better soil quality than either MR or MZ over 2019 and 2020, with higher nitrate-nitrogen and total nitrogen in the 0–40 cm soil layer. MP improved the soil physicochemical properties more than the other treatments, producing significantly higher root numbers and root biomass for the aerial and underground nodal roots than MR and MZ. At harvest, MP had the highest root biomass density,root length density, and root surface area density in the different soil layers(0–20, 20–40, and 0–40 cm). Significant correlations occurred between root biomass and aboveground nitrogen accumulation during maize growth. During grain filling, MP had the greatest nitrogen transfer amount, significantly increasing root and aboveground nitrogen transfer by 19.63–45.82% and 11.15–24.56%, respectively, relative to the other treatments. MP significantly produced 1.36–26.73%higher grain yields and a higher grain crude protein content at harvest than MR and MZ. MP also had higher values for the nitrogen harvest index, nitrogen uptake efficiency, and partial factor productivity of nitrogen fertilizer than MR and *** conclusion, plow tillage combined with a ridge–furrow plastic film mulch system facilitated maize root development and improved nitrogen utilization, thereby increasing maize yield more than the other treatments.

关键词： maize tillage plastic film mulch root nitrogen transfer

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SQoE KQIs Anomaly Detection in Cellular Networks: Fast Online Detection Framework with Hourglass Clustering

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China Communications 2018年第10期15卷 25-37页

作者： Xiaowei qin Shuang tang Xiaohui Chen DANDan miao Guo Wei Key Laboratory of Wireless-Optical Communications Chinese Academy of Sciences School of Information Science and TechnologyUniversity of Science and Technology of China

The explosive growth of data volume in mobile networks makes fast online diagnose a pressing search problem. In this paper, an object-oriented detection framework with a two-step clustering, named as Hourglass Clustering, is given. Where three object parameters are chosen as Synthetical Quality of Experience（SQo E） Key Quality Indicators（KQIs） to reflect accessibility, integrality, and maintainability of networks. Then, we choose represented Key Performance Indicators（r KPIs） as cause parameters with correlation analysis. For these two kinds of parameters, a hybrid algorithm combining the self-organizing map（SOM） and

关键词： big data SQoE anomaly detection hourglass clustering codebook.

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Effect of V–Ti Addition on Microstructure Evolution and Mechanical Properties of Hot-Rolled Transformation-Induced Plasticity Steel

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Acta Metallurgica Sinica(English Letters) 2019年第3期32卷 352-360页

作者： Hai-Cun Yu Zhao-Zhen Cai Gui-qin Fu miao-Yong Zhu School of Metallurgy Northeastern University

In order to reveal the effect of V–Ti addition on the microstructure evolution and the mechanical properties of hot-rolled transformation-induced plasticity(TRIP) steel, two steels with 0.072 V–0.051 Ti steel(Bear-V–Ti steel) and 0.001 V–0.001 Ti steel(Free-V–Ti steel) were designed, respectively, and the comparison analyses were carried out by performing thermodynamic calculation and an experiment. With the thermodynamic calculation, the critical annealing temperature of a large fraction of retained austenite(～51%) obtained via solute enrichment was determined, and an optimized quenching at 650 °C and tempering at 200 °C adopted on the as-hot-rolled steel. The results show that the V–Ti TRIP steel displays more optimum mechanical stability during the tensile deformation, since the fraction and the mechanical stability of retained austenite are improved and the microstructure is also ultrarefined by V–Ti alloy precipitation. The yield strength of Bear-V–Ti steel increases from 650 to 800 MPa, and the ductility reaches 37%, showing that the comprehensive mechanical properties are greatly improved.

关键词： Medium manganese steel Microstructure evolution Mechanical properties V–Ti

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Spatial deduction of mining-induced stress redistribution using an optimized non-negative matrix factorization model

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Journal of Rock Mechanics and Geotechnical Engineering 2023年第11期15卷 2868-2876页

作者： Xu-yan tan Weizhong Chen Luyu Wang Changkun qin State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina

Investigation of mining-induced stress is essential for the safety of coal *** the field monitoring and numerical simulation play a significant role in obtaining the structural mechanical behaviors,the range of monitoring is not sufficient due to the limits of monitoring points and the associated numerical result is not *** this study,we aim to present a spatial deduction model to characterize the mining-induced stress distribution using machine learning algorithm on limited monitoring ***,the framework of the spatial deduction model is developed on the basis of non-negative matrix factorization(NMF)algorithm and optimized by mechanical *** this framework,the spatial correlation of stress response is captured from numerical results,and the learned correlation is employed in NMF as a mechanical constrain to augment the limited monitoring data and obtain the overall mechanical ***,the developed model is applied to a coal mine in Shandong,*** results show the stress distribution in one plane is derived by several monitoring points,where mining induced stress release is observed in goaf and stress concentration in coal pillar,and the intersection point between goaf and coal seam is a sensitive *** indicators used to evaluate the property of the presented model indicate that 83%mechanical performances have been captured and the deduction accuracy is about 92.9%.Therefore,it is likely that the presented deduction model is reliable.

关键词： Machine learning Underground construction Monitoring Mining-induced stress Prediction

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Tree-ring-based winter temperature reconstruction for East Asia over the past 700 years

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Science China Earth Sciences 2021年第6期64卷 872-889页

作者： miao SONG Bao YANG Fredrik Charpentier LJUNGQVIST Feng SHI Chun qin Jianglin WANG Key Laboratory of Desert and Desertification Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou730000China University of Chinese Academy of Sciences Beijing100049China CAS Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of SciencesBeijing100101China Department of History Stockholm UniversityStockholmSE-10691Sweden Bolin Centre for Climate Research Stockholm UniversityStockholmSE-10691Sweden Swedish Collegium for Advanced Study LinneanumThunbergsvägen 2UppsalaSE-75238Sweden Key Laboratory of Cenozoic Geology and Environment Institute of Geology and GeophysicsChinese Academy of SciencesBeijing100029China CAS Center for Excellence in Life and Paleoenvironment Beijing100044China

Almost all proxy-based temperature reconstructions for East Asia have hitherto been designed to resolve summer or annual temperature *** for the winter temperature is still ***,we report an annually resolved,winter-season(December-February,DJF)temperature field reconstruction for East Asia covering the period 1300-2000 CE,based on 260 temperature-sensitive tree-ring *** most striking feature of our new reconstruction is a significant longterm warming trend since the 14th century,which is associated with winter solar insolation at mid-latitudes of the Northern Hemisphere and the global anthropogenic *** amplitude of reconstructed winter temperature change over the study period was~4.7 times greater than that for summer temperature,and the rate of winter temperature increase was~6 times as much as that of summer *** results from climate model simulations were consistent with the reconstruction,showing that the amplitude and rate of temperature change in winter were greater than those in *** reconstruction also suggests the possible influence of volcanic eruptions,anthropogenic activities and winter solar insolation on the winter temperature *** result also suggests a long-term decrease in summer-to-winter temperature difference occurred in 1625(±24 years)CE.

关键词： Tree rings Winter temperature reconstruction Temperature seasonality East Asia

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特发性间质性肺炎病人血清STAT3和FOXM1水平与病情程度及预后的关系研究

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安徽医药 2025年第1期29卷 69-74页

作者：王泽凤邱勤谭建东杨黎四川省建筑医院呼吸肾病科四川成都610084 成都医学院第一附属医院呼吸与危重医学科四川成都610500

目的探讨特发性间质性肺炎(IIP)病人血清信号转导及转录激活因子3(STAT3)、叉头框转录因子M1(FOXM1)表达水平与其病情程度和预后的关系。方法选取2017年1月至2022年10月四川省建筑医院收治的298例IIP病人为IIP组,选取同时期该院收治的29... 详细信息

目的探讨特发性间质性肺炎(IIP)病人血清信号转导及转录激活因子3(STAT3)、叉头框转录因子M1(FOXM1)表达水平与其病情程度和预后的关系。方法选取2017年1月至2022年10月四川省建筑医院收治的298例IIP病人为IIP组,选取同时期该院收治的298例普通型间质性肺炎(UIP)病人为UIP组,另选取同时期在该院体检的280例健康人员为对照组。采用酶联免疫吸附测定(ELISA)检测血清STAT3与FOXM1表达水平,并采用全肺纤维化高分辨率CT(HRCT)评分评价IIP病情严重程度。分析三组受试者血清STAT3与FOXM1表达水平差异。对IIP组病人随访3个月,根据其生存情况分为预后良好组与预后不良组,分析预后不良与预后良好组IIP病人血清STAT3与FOXM1表达水平差异,并采用Spearman法分析IIP病人血清STAT3与FOXM1表达水平与全肺纤维化HRCT评分之间的关系。采用logistic回归分析IIP病人预后不良的影响因素,绘制受试者操作特征曲线(ROC曲线)评估血清STAT3与FOXM1表达水平对IIP病人预后不良的预测效能。结果IIP组、UIP组、对照组血清STAT3[(1.50±0.39)ng/L、(1.32±0.31)ng/L、(1.01±0.27)ng/L]、FOXM1[(34.56±5.64)ng/L、(22.69±4.11)ng/L、(15.51±3.94)ng/L]水平均依次降低(P<0.05)。IIP病人随访3个月共有41例病人死亡,预后不良发生率为13.76%(41/298);预后不良组IIP病人血清STAT3、FOXM1水平均高于预后良好组(P<0.05)。Spearman相关性分析表明,IIP病人血清STAT3、FOXM1水平均与全肺纤维化HRCT评分正相关(rs=0.65,rs=0.57;P<0.001)。另预后不良组年龄、Ⅱ型肺泡细胞表面抗原(KL-6)、白细胞计数、红细胞沉降率(ESR)、C反应蛋白(CRP)水平、全肺纤维化HRCT评分均高于预后良好组(P<0.05);第一秒用力呼气量(FEV1)、最大自主通气量(MVV)均低于预后良好组(P<0.05)。多因素logistic回归分析显示,年龄、KL-6、白细胞、ESR、CRP、STAT3、FOXM1水平、全肺纤维化HRCT评分均是导致IIP病人预后不良的危险因素(P<0.05),FEV1、MVV为保护因素(P<0.05)。ROC分析结果表明,血清STAT3、FOXM1水平联合预测IIP病人预后的灵敏度高于STAT3、FOXM1单独预测的灵敏度(χ^(2)=8.10、6.12,P=0.002、0.008);联合预测的曲线下面积(AUC)高于STAT3、FOXM1单独预测的AUC(Z=3.15、2.54,P=0.002、0.011)。结论IIP病人血清STAT3、FOXM1表达水平均上升,二者与IIP病情程度正相关,均对IIP病人预后具有良好的预测价值,且联合预测效能更高。

关键词：特发性间质性肺炎信号转导及转录激活因子3 叉头框转录因子M1 病情程度预后相关性

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The Shortest First Coordination Mechanism for a Scheduling Game with Parallel-Batching Machines

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Journal of the Operations Research Society of China 2016年第4期4卷 517-527页

作者： qing-qin Nong Sai-Jun Guo Li-Hui miao School of Mathematical Science Ocean University of ChinaQingdao 266100ShandongChina

This paper deals with a scheduling problem with parallel-batching machines from a game theoretic *** are m parallel-batching machines,each of which can handle up to b jobs simultaneously as a *** processing time of a batch is the time required for processing the longest job in the batch,and all the jobs in a batch start and complete at the same *** are n *** job is owned by a rational and selfish *** agent of a job chooses a machine for processing its own *** aim of each agent is to minimize the completion time of its job while the system tries tominimize the maximal completion time over all jobs,the *** design a coordination mechanism for the scheduling game *** discuss the existence of Nash equilibria of the mechanism and showthat it has a price of anarchy 2.

关键词： Scheduling Game Coordination mechanism Nash equilibrium Price of anarchy

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The incentive and coordination strategy of sustainable construction supply chain based on robust optimisation

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Journal of Control and Decision 2020年第2期7卷 126-159页

作者： Ya-qin Lin Chun-xiang Guo Yue tan Antai College of Economics&Management Shanghai Jiao Tong UniversityShanghaiPeople’s Republic of China Business School of Sichuan University Chengdu 610064People’s Republic of China

This paper considers the incentive and coordination problem of the construction supply chain consisting of the owner,general contractor,subcontractors and suppliers based on the sustainable development goals under uncertain ***,according to the general contract management pattern and the relationships among those participants,we establish an uncertain bi-level nonlinear model in which the owner set the proper intensities to minimise the total cost at first while the general contractor decides its alternative and the limits to subcontractors ***,we convert the primal model into a deterministic bi-level nonlinear programming model based on the concept of relative ***,a solution procedure combing interactive fuzzy technique and genetic algorithm is ***,the validity of the model is verified by a numerical *** paper provides a method to optimise the construction project management from the perspective of supply chain under the requirement of sustainable development.

关键词： Sustainable development construction supply chain incentive coordination bi-level programming robust optimisation

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