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铜纤维毡的模板-热还原法制备及二氧化碳电还原性能

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河北科技师范学院学报 2024年第2期38卷 66-72页

作者：张璐沈子千王若珊宋苗兰帅申偲伯河北科技师范学院化学工程学院河北省天然产物活性成分与功能重点实验室河北秦皇岛066600

为了研究人工碳纳米材料模板对铜纤维毡制备及结构的影响,应用模板-热还原法,以3种碳材料为模板,通过优化前驱体中CuCl_(2)·2H_(2)O与模板的质量比尝试制备铜纤维毡。使用X射线衍射仪、扫描电子显微镜和能量色散谱仪分析了试样的结构... 详细信息

为了研究人工碳纳米材料模板对铜纤维毡制备及结构的影响,应用模板-热还原法,以3种碳材料为模板,通过优化前驱体中CuCl_(2)·2H_(2)O与模板的质量比尝试制备铜纤维毡。使用X射线衍射仪、扫描电子显微镜和能量色散谱仪分析了试样的结构和化学组成。结果表明,石墨烯可发挥模板作用,得到橙红色厘米级铜纤维毡,铜纤维毡中铜纤维的直径约5μm,且铜纤维表面分布亚微米级台阶。将铜纤维毡制成自支撑电极,应用在线气相色谱仪和核磁共振波谱仪评价了铜纤维毡的CO_(2)电化学还原性能。相对于泡沫铜,铜纤维毡作为CO_(2)电还原反应的催化剂,展现了对甲酸生成较高的选择性。

关键词：铜纤维毡模板-热还原法石墨烯二氧化碳电还原性能

Growth inhibition induced by short hairpin RNA to silence survivin gene in human pancreatic cancer cells

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Hepatobiliary & Pancreatic Diseases International 2010年第1期9卷 69-77页

作者： Shen, Yong-Mei Yang, Xiao-Chun song, miao-Li Qin, Chen-Hao Yang, Chen Sun, Yi-Hui Soochow Univ Dept MRI Affiliated Hosp 2 Suzhou 215004 Peoples R China Soochow Univ Dept Gen Surg Affiliated Hosp 2 Suzhou 215004 Peoples R China Municipal Hosp Suzhou Radioimmunoassay Ctr Suzhou 215002 Peoples R China Soochow Univ Radioimmunoassay Ctr Affiliated Hosp 2 Suzhou 215004 Peoples R China Soochow Univ Clin Lab Affiliated Hosp 2 Suzhou 215004 Peoples R China

BACKGROUND: Survivin is known to be overexpressed in various human malignancies, including pancreatic cancer, and mediates cancer cell proliferation and tumor growth, so the regulation of this molecule could be a new strategy for treating pancreatic cancer. In this study, short hairpin RNAs (shRNAs) specific to survivin were introduced into human pancreatic cancer Patu8988 cells to investigate the inhibitory effects on survivin expression and cell proliferation in vitro and in vivo. METHODS: Three kinds of shRNA specific to the survivin gene were designed and cloned into eukaryotic expression plasmid pGenesil-1 vector. Subsequently the recombinant plasmids were transfected into human pancreatic cancer Patu8988 cells with lipfectamine (TM) 2000 reagent. The mRNA and protein expressions of survivin in the transiently transfected Patu8988 cells were determined by RT-PCR, flow cytometry, and Western blotting analysis. The proliferation inhibition rates of stably transfected Patu8988 cells were determined by MTT assay. The antitumor activities of the three kinds of survivin-shRNA plasmids were evaluated in BALB/c nude mice inoculated with Patu8988 cells and bearing human pancreatic cancer. RESULTS: The three survivin-shRNA plasmids named pGenesil-1-survivin-1, pGenesil-1-survivin-2 and pGenesil-1-survivin-1+2 (with double interfering RNA sites) were successfully constructed, and were confirmed by restriction enzyme cutting and sequencing. At 48 hours after transfection, the expression of survivin mRNA and protein was inhibited in Patu8988 cells transfected with pGenesil-1-survivin-1, pGenesil-1-survivin-2, and pGenesil-1-survivin-1+2 when compared with that of either pGenesil-1-NC (with scrambled small interfering RNA) transfected cells or control cells (P<0.05). The MTT results showed that the proliferation rates of Patu8988 cells stably transfected with survivin-shRNA plasmids were reduced when compared with that of either pGenesil-1-NC transfected cells or control cel

关键词： pancreatic neoplasms short hairpin RNA survivin pGenesil-1 vector

An all-in-one FeO_(x)-rGO sponge fabricated by solid-phase microwave thermal shock for water evaporation and purification

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Journal of Environmental Sciences 2024年第4期138卷 671-683页

作者： Youkun Sun Xiuwen Zhao Xueling song Jinchen Fan Junhe Yang Yingchun miao Shuning Xiao School of Materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China College of Chemistry and Environmental Science Qujing Normal UniversityQujing 655011China Prytula Igor Collaborate Innovation Center for Diamond Shanghai Jian Qiao UniversityShanghai 201306China

Developing high-efficiency photothermal seawater desalination devices is of significant importance in addressing the shortage of *** much effort made into photothermal materials,there is an urgent need to design a rapidly synthesized photothermal evaporator for the comprehensive purification of complex ***,we report on all-in-one FeOx-rGO photothermal sponges synthesized via solid-phase microwave thermal *** narrow band gap of the semiconductor material Fe_(3)O_(4) greatly reduces the recombination of electron-hole pairs,enhancing non-radiative relaxation light *** abundantπorbitals in rGO promote electron excitation and thermal vibration between the *** of the surface hydrophilicity and hydrophobicity promotes salt resistance while simultaneously achieving the purification of various complex polluted *** optimized GFM-3 sponge exhibitedan enhanced photothermal conversion rate of 97.3% and a water evaporation rate of 2.04 kg/(m^(2)·hr),showing promising synergistic water purification *** findings provide a highly efficient photothermal sponge for practical applicationsof seawater desalination and purification,as well as develop a super-rapid processing methodology for evaporation devices.

关键词： Desalination Photothermal device Microwave synthesis Water purification FeO_(x)-rGO

高尿酸血症对雌性大鼠生殖功能的影响及其机制

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精准医学杂志 2024年第3期39卷 213-217页

作者：龚雪琳邹通宋淼刘呈杨张清孙钰邢士超青岛大学基础医学院病原生物学系山东青岛266071 青岛大学附属青岛妇女儿童医院

目的探讨高尿酸血症(HUA)对雌性大鼠生殖功能的影响及其机制。方法雌性Wistar大鼠18只,随机分为3组,每组各6只。高尿酸组(HUA组)和高尿酸恢复组(HR组)大鼠每天灌胃50 mg/kg尿酸及腹腔注射250 mg/kg氧嗪酸钾,至第9周时,HR组大鼠改用等体... 详细信息

目的探讨高尿酸血症(HUA)对雌性大鼠生殖功能的影响及其机制。方法雌性Wistar大鼠18只,随机分为3组,每组各6只。高尿酸组(HUA组)和高尿酸恢复组(HR组)大鼠每天灌胃50 mg/kg尿酸及腹腔注射250 mg/kg氧嗪酸钾,至第9周时,HR组大鼠改用等体积生理盐水进行灌胃和腹腔注射,HUA组不变,再继续处理7周;对照组(N组)大鼠给予相同体积的生理盐水灌胃及腹腔注射,持续16周。第16周时,测量三组大鼠体质量和子宫、卵巢质量,并计算子宫、卵巢系数;苏木精-伊红染色法观察3组大鼠子宫、卵巢组织的病理变化;酶联免疫吸附试验法检测3组大鼠血清中促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)含量;使用阴道脱落细胞涂片法观察3组大鼠动情周期;采用比色法检测3组大鼠子宫组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)水平;采用免疫荧光法检测3组大鼠子宫组织中肿瘤坏死因子α(TNF-α)的荧光强度。结果与N组大鼠相比,HUA组大鼠子宫系数降低、卵泡数量减少、子宫内膜厚度变薄,动情间期缩短,血清FSH水平增加,子宫组织中SOD、GSH水平降低,而MDA含量增加(F=7.80~111.40,t=3.95~12.37,P<0.05),子宫组织中TNF-α荧光强度增高;与HUA组大鼠相比,HR组卵泡数量显著增多、子宫内膜显著变厚,血清中FSH水平增高,子宫组织中SOD、GSH水平显著增高,而MDA水平显著降低(t=3.40~11.56,P<0.05),子宫组织中TNF-α荧光强度降低。结论HUA可致雌性大鼠生殖功能下降,其可能机制与大鼠血清尿酸水平升高,致性激素分泌紊乱、机体氧化应激水平增高,进而诱导炎症反应发生有关。

关键词：高尿酸血症动情期性腺甾类激素细胞因子类炎症氧化性应激女(雌)性大鼠,Wistar

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Mitochondrial diseases and mtDNA editing

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Genes & Diseases 2024年第3期11卷 303-310页

作者： Min song Lingqun Ye Yongjin Yan Xuechun Li Xinglong Han Shijun Hu miao Yu Department of Cardiovascular Surgery of the First Affiliated Hospital&Institute for Cardiovascular Science Collaborative Innovation Center of HematologyState Key Laboratory of Radiation Medicine and ProtectionSuzhou Medical CollegeSoochow UniversitySuzhouJiangsu 215000China Hai'an People's Hospital NantongJiangsu 226600China

Mitochondrial diseases are a heterogeneous group of inherited disorders character-ized by mitochondrial dysfunction,and these diseases are often severe or even ***-chondrial diseases are often caused by mitochondrial DNA ***,there is no curative treatment for patients with pathogenic mitochondrial DNA *** the rapid development of traditional gene editing technologies,such as zinc finger nucleases and tran-scription activator-like effector nucleases methods,there has been a search for a mitochon-drial gene editing technology that can edit mutated mitochondrial DNA;however,there are still some problems hindering the application of these *** discovery of the DddA-derived cytosine base editor has provided hope for mitochondrial gene *** this paper,we will review the progress in the research on several mitochondrial gene editing technologies with the hope that this review will be useful for further research on mitochondrial gene editing technologies to optimize the treatment of mitochondrial diseases in the future.

关键词： Gene editing Mitochondrialdisease MitochondrialDNA mutation Transcription activator-like effectornucleases Zinc finger nucleases

The effect of polyvinylpyrrolidone addition on microstructure,surface aspects,the glass transition temperature and structural strength of honey and coconut sugar powders

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Journal of Future Foods 2022年第4期2卷 338-345页

作者： Valentyn A.Maidannyk Vinay S.N.Mishra song miao Mohamad Djali Noel McCarthy Bambang Nurhadi Food Chemistry and Technology Department Teagasc Food Research CentreMooreparkFermoy P61 C996Ireland Department of Food Industrial Technology Faculty of Agro-Industrial TechnologyUniversitas PadjadjaranBandung 40600Indonesia Study Centre of Agric.Technology Development Faculty of Agric.Industrial TechnologyUniversitas PadjadjaranBangung 40600Indonesia

Deep knowledge of the microstructure and physicochemical properties of polymeric food systems,such as honey powders(HP)and coconut sugars(CS),has practical importance for industry and end *** study investigated the effect of polyvinylpyrrolidone(PVP)addition on microstructure,roughness,glass transition,α-relaxation temperatures and structural strength(S)of anhydrous complex carbohydrates *** addition of PVP slightly increased the glass transition andα-relaxation temperature for HP and CS systems as high molecular weight *** with PVP addition showed“stronger”behaviour according to the S *** light,scanning electron and atomic force microscopies showed slight differences in transparency and shapes between controls and systems with PVP *** morphological changes and roughness were investigated in this study to provide insight into HP and CS particles’structural ***,S-involved structural diagrams were built to determine S parameters for controlling the structural transformation of HP and CS systems with and without PVP *** results obtained in this work provide new information on polymer-carbohydrate interactions in complex food systems and structural transformations during their production and storage.

关键词： Honey powder Coconut sugar powder Structural strength Scanning electron microscopy Atomic force microscopy Glass transition Structural relaxation

Atomically dispersed calcium as solid strong base catalyst with high activity and stability

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Green Energy & Environment 2024年第10期9卷 1619-1626页

作者： Xiang-Bin Shao Zhi-Wei Xing Si-Yu Liu Ke-Xin miao Shi-Chao Qi song-song Peng Xiao-Qin Liu Lin-Bing Sun State Key Laboratory of Materials-Oriented Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)College of Chemical EngineeringNanjing Tech University30 South Puzhu Road

Solid strong base catalysts are highly attractive for diverse reactions owing to their advantages of neglectable corrosion,facile separation,and environmental ***,their widespread applications are impeded by basic components aggregation and low *** this work,we fabricate single calcium atoms on graphene （denoted as Ca1/G） by use of a redox strategy for the first time,producing solid strong base catalyst with high activity and *** precursor Ca（NO3）2is first reduced to CaO at 400℃ by the support graphene,forming CaO/G with conventional basic sites,and the subsequent reduction at 850℃ results in the generation of Ca1/G with atomically dispersed *** characterizations reveal that Ca single atoms are anchored on graphene in tetra-coordination （Ca–C2–N2） where N is in situ doped from Ca（NO3）*** atomically dispersed Ca,along with their anchoring on the support,endow Ca1/G with high activity and stability toward the transesterification reaction of ethylene carbonate with *** turnover frequency value reaches 128.0 h-1on Ca1/G,which is much higher than the traditional counterpart CaO/G and various reported solid strong bases (2.9–46.2 h-1).Moreover,the activity of Ca1/G is well maintained during 5 cycles,while 60% of activity is lost for the conventional analogue CaO/G due to the leaching of Ca.

关键词： Solid strong base Single Ca atoms Heterogeneous catalysts Transesterification Reaction

Plasma Membrane Localization and Potential Endocytosis of Constitutively Expressed XA21 Proteins in Transgenic Rice

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Molecular Plant 2010年第5期3卷 917-926页

作者： Fang Chen Ming-Jun Gao Yan-song miao Yue-Xing Yuan Mu-Yang Wang Qun Li Bi-Zeng Mao Li-Wen Jiang Zu-Hua He National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Present address: Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06511 USA Department of Biology and Molecular Biotechnology Program The Chinese University of Hong Kong Shatin New Territories Hong Kong China Present address: Department of Emergency Medicine Thomas Jefferson University Philadelphia PA 19107 USA Biotechnology Institute Zhejiang University Hanghzou 310029 China

The rice pattern recognition receptor （PRR） XA21 confers race-specific resistance in leaf infection by bacterial blight Xathornonas oryzae pv. oryzae （Xoo）, and was shown to be primarily localized to the endoplasmic reticulum （ER） when expressed with its native promoter or overexpressed in the protoplast. However, whether the protein is still ER- localization in the intact cell when overexpressed remains to be identified. Here, we showed that XA21, its kinase-dead mutant XA21PK736EP and the triple autophosphorylation mutant XA21PS686AJT688AJS699A GFP fusions were primarily localized to the plasma membrane （PM） when overexpressed in the intact transgenic rice cell, and also localized to the ER in the transgenic protoplast. The transgenic plants constitutively expressing the wild-type XA21 or its GFP fusion displayed racespecific resistance to Xoo at the adult and seedling stages. XA21 and XA21PK736EP could be internalized probably via the SCAMP-positive early endosomal compartment in the protoplast, suggesting that XA21 might be endocytosed to initiate resistance responses during pathogen infection. We also established a root infection system and demonstrated that XA21 also mediated race-specific resistance responses to Xoo in the root. Our current study provides an insight into the nature of the XA21-mediated resistance and a practical approach using the root cell system to further dissect the cellular signaling of the PRR during the rice-Xoo interaction.

关键词： Rice XA21 pattern recognition receptor bacterial bight cellular localization internalization root infection race-specific resistance.

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海鲜菇美拉德肽的制备及其风味特性研究

食品研究与开发 2024年第3期45卷 146-155页

作者：林钦淋黄焰峰方焕新林波月缪松林灼华邓凯波福建农林大学食品科学学院福建福州350002 中国-爱尔兰国际合作食品物质学与结构设计研究中心福建福州350002 福建省特种淀粉品质科学与加工技术重点实验室福建福州350002 爱尔兰农业部Teagasc食品研究中心爱尔兰科克119634 庄臣酿酒(福建)有限公司福建福州350500

以海鲜菇(Hypsizygus marmoreus)蛋白肽为原料,采用响应面法分析优化海鲜菇美拉德肽制备工艺,并通过褐变程度、产物分子量分布、感官评价、电子舌及超滤等特征性指标和评价手段,明确美拉德反应对海鲜菇蛋白肽风味特性的影响。结果表明,... 详细信息

以海鲜菇(Hypsizygus marmoreus)蛋白肽为原料,采用响应面法分析优化海鲜菇美拉德肽制备工艺,并通过褐变程度、产物分子量分布、感官评价、电子舌及超滤等特征性指标和评价手段,明确美拉德反应对海鲜菇蛋白肽风味特性的影响。结果表明,海鲜菇美拉德肽的最佳制备条件为葡萄糖与D-木糖质量比4∶1、反应温度110℃和反应时间56 min。与海鲜菇蛋白肽相比,制备的海鲜菇美拉德肽咸味强烈,并伴有鲜味产生,整体接受度好,在294 nm和420 nm下吸光值显著增加,并推测分子量大于180~500 Da的组分可能为美拉德反应的主要活性肽段。超滤结果表示,海鲜菇美拉德肽呈咸鲜味的肽分子量主要集中在500 Da以下。该研究结果证明美拉德反应可有效提升海鲜菇蛋白肽的咸鲜味,使其风味层次更加丰富,可为扩展可替代钠盐的新型食用菌调味领域的研究和应用提供理论基础。

关键词：海鲜菇蛋白肽美拉德反应产物分子量分布风味特性咸鲜味替代钠盐