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Qualitative and quantitative detection using eDNA technology: A case study of Fenneropenaeus chinensis in the Bohai Sea

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Aquaculture and Fisheries 2020年第3期5卷 148-155页

作者： miao lia Xiujuan Shan Weiji Wang Xiaosong Ding Fangqun Dai Ding Lv Huanhuan Wu Key Laboratory of Sustainable Development of Ma Fisheries Ministry of Agriculture and Rural AffairsShandong Provincial Key Laboratory of Fishery Resources and Ecological EnvironmentYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266237China

Accurate knowledge of species distributions and population dynamics is the basis for conservation ***, for certain species with unique life histories and very small populations, monitoring species distributions and population dynamics is extremely difficult. Recently, newly developed eDNA technology has been widely used in species monitoring, biodiversity assessments, and biomass assessments. In this research, we studied Fenneropenaeus chinensis in the Bohai Sea. We used a 0.45 μm glass fiber filter combined with a vacuum to filter 2 L seawater samples for eDNA enrichment from 54 stations in June and 60 stations in August. A DNeasy Blood and Tissue kit was used to extract the eDNA. After DNA extraction, the specific primers and probes of the mitochondrial DNA COI gene of F. chinensis were designed. Real-time quantitative PCR was used to qualitatively and quantitatively analyze the eDNA in the Bohai Sea. The temporal and spatial distribution of F. chinensis was detected by the eDNA technique. The relationship between the eDNA copy number and trawl-netted F. chinensis biomass was explored. The applicability and sensitivity of eDNA technology in marine crustacean research were verified. The results showed that the mtDNA COI gene of F. chinensis was successfully amplified in the 54 water samples collected in June, and the detection rate reached 100%. Detections occurred in only 23 of the 60 stations sampled in August. F. chinensis had a detection rate of only 38%. The copy number of eDNA obtained by realtime PCR was fitted to the density of F. chinensis captured by bottom trawling. There was not a significant positive correlation between copy number and biomass. The results obtained through this research will provide a method and theoretical basis for the application of eDNA technology in marine crustacean research.

关键词： Bohai Sea eDNA Fenneropenaeus chinensis Spatial and temporal distribution Biomass

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Identification of Prophages within the Mycobacterium avium 104 Genome and the Link of Their Function Regarding to Environment Survival

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Advances in Microbiology 2016年第13期6卷 927-941页

作者： miao Zhao Kerrigan Gilbert lia Danelishvili Brendan Jeffrey Luiz E. Bermudez Program of Biology Oregon State University Corvallis OR USA Department of Biomedical Sciences College of Veterinary Medicine Oregon State University Corvallis OR USA Department of Microbiology Oregon State University Corvallis OR USA

Mycobacterium avium is an opportunistic bacterium associated with pathogenic behavior in both humans and animals. M. avium has evolved as a pathogen by having an environmental component in its life style. Prophages are the integrated viral forms in bacterium genome. They constitute about 10% - 20% of genome of many bacteria and they contribute to pathogenicity of microbes. We investigated whether the M. avium 104 genome contained prophages and evaluated the genes/proteins for putative functions. Three prophage genes were identified in the M. avium 104 database, and sequences were analyzed for specific motifs. The prophage sequences were then cloned in Mycobacterium smegmatis and the bacterial phenotype was evaluated in gain of function assays for environmental stresses, such as tolerance to extreme temperatures, UV light, biofilm formation and resistance to acid as well as macrophage survival. The results indicate that two of the prophage genes, MAV_0696 and MAV_2265, confer M. smegmatis with enhanced ability to produce biofilm. Using a Real-Time PCR, it was determined that MAV_0696 and MAV_2265 transcripts were upregulated upon biofilm formation by M. avium. The expression of MAV_2265 gene was significantly higher at all selected time points. In addition, the expression of MAV_2265 in M. smegmatis also led to significantly greater survival rate at pH 5.0 compared to the wild-type control. None of the other physical abilities were altered by overexpressing the prophage genes in M. smegmatis. In summary, we identified three prophage sequences in M. avium 104, from which two of them were found to be associated with biofilm formation and one with resistance to the acidic environment. Future studies will identify the mechanisms involved in the prophages function.

关键词： Mycobacterum avium Prophage Virulence Environment

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Identification of annual routines and critical stopover sites of a breeding shorebird in the Yellow Sea,China

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Avian Research 2022年第4期13卷 407-414页

作者： Yang Wu Weipan Lei Bingrun Zhu Jiaqi Xue Yuanxiang miao Zhengwang Zhang Key Laboratory for Biodiversity Science and Ecological Engineering College of Life SciencesBeijing Normal UniversityBeijing100875China Demonstration Center for Experimental Life Sciences&Biotechnology Education College of Life SciencesBeijing Normal UniversityBeijing100875China Conservation Ecology Group Groningen Institute for Evolutionary Life Sciences(GELIFES)University of GroningenPO Box 111039700CCGroningenthe Netherlands School of Life Science and Technology Shanghai Tech UniversityShanghai201210China

Pied Avocets(Recurvirostra avosetta)are common migratory shorebirds in the East Asian–Australasian *** 2019 to 2021,GPS/GSM transmitters were used to track 40 Pied Avocets nesting in northern Bohai Bay to identify annual routines and key stopover *** average,southward migration of Pied Avocets started on 23 October and arrived at wintering sites(mainly in the middle and lower reaches of the Yangtze River and coastal wetlands)in southern China on 22 November;northward migration started on 22 March with arrival at breeding sites on 7 *** avocets used the same breeding sites and wintering sites between years,with an average migration distance of 1124 *** was no significant difference between sexes on the migration timing or distance in both northward and southward migration,except for the departure time from the wintering sites and winter *** coastal wetland of lianyungang in Jiangsu Province is a critical stopover *** individuals rely on lianyungang during both northward and southward migration,indicating that species with short migration distances also heavily rely on a few stopover ***,lianyungang lacks adequate protection and is facing many threats,including tidal flat *** strongly recommend that the coastal wetland of lianyungang be designated as a protected area to effectively conserve the critical stopover site.

关键词： Bohai bay EAAF lianyungang Migration Recurvirostra avosetta

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公共卫生事件的心理应激与干预策略

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南昌大学学报（人文社会科学版） 2005年第3期36卷 49-52页

作者：朱蕴丽苗元江江西医学院社会科学教研部江西南昌330006 江西医学院心理咨询中心江西南昌330006

公共卫生事件的心理应激及心理干预是现代公共卫生研究的焦点课题,突发性公共卫生事件导致公众心理应激反应,并对个人、经济与社会产生消极影响。针对公共卫生事件,应从以下几个方面进行心理应激干预:一是建立危机干预系统,积极开展心... 详细信息

公共卫生事件的心理应激及心理干预是现代公共卫生研究的焦点课题,突发性公共卫生事件导致公众心理应激反应,并对个人、经济与社会产生消极影响。针对公共卫生事件,应从以下几个方面进行心理应激干预:一是建立危机干预系统,积极开展心理援助;二是开展心理应激研究,构建社会支持系统;三是造就积极心理品质,培养公众的心理承受能力。

关键词：心理应激干预策略突发性公共卫生事件社会支持系统心理承受能力经济与社会心理干预应激反应公众心理消极影响危机干预心理援助心理品质

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Promising electroplating solution for facile fabrication of Cu quantum point contacts

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Nano Research 2017年第10期10卷 3314-3323页

作者： Yang Yang Junyang Liu Jueting Zheng miao Lu Jia Shi Wenjing Hong Fangzu Yang Zhongqun Tian State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical Engineering Pen-Tung Sah Institute ot Micro-Nano Science and Technology LIA CNRS NanoBioCatEchem Collaborative Innovation Center of Chemistry for Energy Materials Xiamen University Kiamen 361005 China

In this article, we report on the fabrication and transport measurements of Cu quantum point contacts prepared by a novel, electrochemically assisted mechanically controllable break junction （EC-MCBJ） method. By employing photolithography and wet-etching processes, suspended electrode pairs were patterned and fabricated successfully on Si microchips. Rather than adopting an acid Cu electroplating solution, a novel alkaline electroplating solution was developed and utilized to establish Cu nanocontacts between electrode pairs. Typically, the widths of the as-fabricated Cu nanocontacts were found to be smaller than 18 nm. A large number of Cu quantum point contacts were then produced and characterized by a home-built MCBJ setup. In addition to the conventional histogram, where peaks tend to decrease in amplitude with increasing conductance, an anomalous type of conductance histogram, exhibiting different peak amplitudes, was observed. Through statistical analysis of the maximum allowable bending of the Si microchips, and theoretical calculations, we demonstrated that our alkaline Cu electroplating solution affords Cu nanocontacts that are compatible with subsequent MCBJ operations, which is essential for the fabrication of Cu quantum point contacts. As sophisticated e-beam lithography is not required, the EC-MCBJ method is fast, simple, and cost-effective. Moreover, it is likely to be suitable for the fabrication and characterization of quantum point contacts of various metals from their respective electroplating solutions.

关键词： quantum point contact electrochemical deposition Cu nanowire mechanically controllablebreak junction (MCBJ) conductance quantization

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全身麻醉和硬膜外麻醉对老年骨科患者术后短期认知功能的影响

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中华老年医学杂志 2012年第2期31卷 144-146页

作者：夏燕飞黄浩周嘉莉罗妙妙浙江医院麻醉科杭州310013

目的观察全身麻醉和硬膜外麻醉对老年骨科患者术后短期认知功能的影响. 方法选择全髋关节置换或股骨骨折切复内固定手术患者120例,美国麻醉医师协会（ASA）病情分级Ⅰ、Ⅱ级,数字抽签随机分为全身麻醉组和硬膜外麻醉组各60例.记录术... 详细信息

目的观察全身麻醉和硬膜外麻醉对老年骨科患者术后短期认知功能的影响. 方法选择全髋关节置换或股骨骨折切复内固定手术患者120例,美国麻醉医师协会（ASA）病情分级Ⅰ、Ⅱ级,数字抽签随机分为全身麻醉组和硬膜外麻醉组各60例.记录术前、麻醉后手术前、手术30 min、主要手术步骤操作时、手术结束时等不同时点平均动脉压和心率,采用简易精神状况检查（MMS）测定两组患者不同时间点的认知功能. 结果两组患者术中平均动脉压、心率比较,差异无统计学意义（均P＞0.05）.MMS评分全身麻醉组麻醉结束6、12、24 h[分别为（26.5±0.5）、（25.4±0.7）、（27.4±0.3）分]与麻醉诱导前[（29.5±0.3）分]比较,差异有统计学意义（均P＜0.05）,与麻醉结束72 h[（29.2±0.3）分]比较,差异无统计学意义（P＞0.05）；硬膜外麻醉组麻醉结束6、12 h[分别为（26.6±0.4）、（25.6±0.8）分]与麻醉诱导前[（29.4±0.4）分]比较,差异有统计学意义（均P＜0.05）,麻醉结束24、72 h[分别为（29.1±0.4）、（29.5±0.4）分]与麻醉诱导前比较,差异无统计学意义（P＞0.05）.在麻醉结束后24 h,硬膜外麻醉组患者的MMS评分为（29.1±0.4）分,高于全身麻醉组（27.4±0.3）分,差异有统计学意义（P＜0.01）. 结论全身麻醉在术后12 h内对老年骨科患者认知功能的影响较硬膜外麻醉更为明显.

关键词：麻醉,全身麻醉,硬膜外认知障碍

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Synthesis of selective PAK4 inhibitors for lung metastasis of lung cancer and melanoma cells

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Acta Pharmaceutica Sinica B 2022年第6期12卷 2905-2922页

作者： Peilu Song Fan Zhao Dahong Li Jiqiang Qu miao Yao Yuan Su Hanxun Wang miaomiao Zhou Yujie Wang Yinli Gao Feng Li Dongmei Zhao Fengjiao Zhang Yu Rao Mingyu Xia Haitao Li Jian Wang Maosheng Cheng Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of Education Shenyang Pharmaceutical UniversityShenyang 110016China MOE Key Laboratory of Protein Sciences School of Pharmaceutical SciencesMOE Key Laboratory of Bioorganic Phosphorus Chemistry Chemical BiologyTsinghua UniversityBeijing 100084China MOE Key Laboratory of Protein Sciences Beijing Advanced Innovation Center for Structural BiologyTsinghua-Peking Joint Center for Life SciencesSchool of Life Sciences and School of MedicineTsinghua UniversityBeijing 100084China School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityShenyang 110016China School of Life Science and Biopharmaceutics Shenyang Pharmaceutical UniversityShengyang 110016China Wuya College of Innovation Shenyang Pharmaceutical UniversityShenyang 110016China Department of Cell Biology Key Laboratory of Cell BiologyMinistry of Public Healthand Key Laboratory of Medical Cell BiologyMinistry of EducationChina Medical UniversityShenyang 110001China

The p21 activated kinase 4(PAK4) is serine/threonine protein kinase that is critical for cancer *** by X-ray crystallography and structure-based optimization,we report a novel subseries of C-3-substituted 6-ethynyl-1 H-indole derivatives that display high potential and specificity towards group Ⅱ *** these inhibitors,compound 55 exhibited excellent inhibitory activity and kinase selectivity,displayed superior anti-migratory and anti-invasive properties against the lung cancer cell line A549 and the melanoma cell line *** 55 exhibited potent in vivo antitumor metastatic efficacy,with over 80% and 90% inhibition of lung metastasis in A549 or B16-BL6 lung metastasis models,*** mechanistic studies demonstrated that compound 55 mitigated TGF-β1-induced epithelial-mesenchymal transition(EMT).

关键词： Anti-cancer PAK4 inhibitor Kinase selectivity Cocrystallization Lung metastasis EMT

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中职学校艺术教育课程在课程体系中地位初探

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佳木斯教育学院学报 2011年第2期 84-85页

作者：刘佳苗素莲广东技术师范学院

"艺术教育课程"是中职学校美育最主要的载体和最有效的途径,是培养德智体美全面发展的社会主义现代化建设需要的高素质技能人才必不可少的。目前中职学校艺术教育课程在课程体系中地位不高,笔者从中职学校中了解艺术教育课程的地位现状... 详细信息

"艺术教育课程"是中职学校美育最主要的载体和最有效的途径,是培养德智体美全面发展的社会主义现代化建设需要的高素质技能人才必不可少的。目前中职学校艺术教育课程在课程体系中地位不高,笔者从中职学校中了解艺术教育课程的地位现状,从观念偏颇、体系杂乱、基础资源缺失等原因进行分析,并提出提高中职学校艺术教育课程地位的思考。

关键词：中职学校艺术教育课程必不可少地位

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炮弹壳体液态模锻模设计

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模具工业 2008年第2期34卷 61-63页

作者：熊洪淼李春王家宣李达南昌航空大学材料学院江西南昌330063

采用液态模锻工艺生产杯形、薄壁深筒形炮弹壳体,依据零件图设计了液态模锻工艺用锻件图、试验用模具,并生产出试验产品。经过试验产品分析,最终设计制造出生产用模具。模具结构简单、紧凑,工作平稳,生产的零件符合设计要求。

关键词：炮弹壳体液态模锻模具设计

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护理风险管理在新生儿病房中的应用效果

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中国当代医药 2015年第20期22卷 172-174页

作者：梁素丽陈妙玲广东省汕尾市第二人民医院新生儿科广东汕尾516600

目的探讨护理风险管理在新生儿病房中的临床应用效果。方法选择2014年2-5月本院新生儿科病房的80例新生患儿作为研究对象,将其随机分为试验组和对照组,各40例。试验组采用护理风险管理措施,对照组采用普通护理措施,比较两组的护理质... 详细信息

目的探讨护理风险管理在新生儿病房中的临床应用效果。方法选择2014年2-5月本院新生儿科病房的80例新生患儿作为研究对象,将其随机分为试验组和对照组,各40例。试验组采用护理风险管理措施,对照组采用普通护理措施,比较两组的护理质量、护理记录合格率、护理纠纷发生率及家长护理满意度等指标。结果试验组的护理质量得分、护理记录合格率、家长满意度分别为（98.8±1.1）分、97.5%、95.0%,显著高于对照组的（87.5±1.5）分、77.5%、72.5%（P〈0.05）;试验组的护理纠纷发生率为2.5%,显著低于对照组的20%（P〈0.05）。结论在新生儿病房中实施护理风险管理,可提高护理质量,降低护理纠纷发生率,保证患儿的生命健康和安全,值得临床推广应用。

关键词：新生儿护理风险管理应用效果

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