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Analysis of Global Expression Profiles of Arabidopsis Genes Under Abscisic Acid and H2O2 Applications

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Journal of Integrative Plant Biology 2006年第1期48卷 62-74页

作者： Peng-Cheng Wang Yan-Yan Du Guo-Yong An Yun Zhou Chen miao Chun-Peng song Laboratory of Plant Stress Biology Department of Biology Henan University Kaifeng 475001 China

To gain insight into the coordination of gene expression profiles under abscisic acid （ABA） and H2O2 applications, global changes in gene expression in response to ABA and H2O2 in Arabidopsis seedlings were investigated using GeneChip （Santa Clara, CA, USA） arrays. Among over 24 000 genes present in the arrays, 459 tran- scripts were found to be significantly increased, whereas another 221 decreased following H2O2 treatment compared with control. Similar to treatment with H2O2, ABA treatment elevated the transcription of 391 genes and repressed that of 322 genes. One hundred and forty-three upregulated genes and 75 downregulated genes were shared between the two treatments and these genes were mainly involved in metabolism, signal transduction, transcription, defense, and resistance. Only two genes, which encode an APETALA2/dehydra- tion-responsive element binding protein （AP2/DREBP） family transcriptional factor and a late embryogenesis- abundant protein, were downregulated by H202, but upregulated by ABA. These results suggest that, similar to ABA, H202 plays a global role in gene transcription of Arabidopsis seedlings. The transcriptional responses induced by the application of exogenous ABA and H2O2 overlapped substantially. These two treatments regulated most of the downstream genes in a coordinated manner.

关键词： abscisic acid gene expression hydrogen peroxide microarray.

Hydrometallurgical recovery of lithium carbonate and iron phosphate from blended cathode materials of spent lithium-ion battery

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Rare Metals 2024年第3期43卷 1275-1287页

作者： Shao-Le song Run-Qing Liu miao-miao Sun Ai-Gang Zhen Fan-Zhen Kong Yue Yang School of Minerals Processing and Bioengineering Central South UniversityChangsha410083China Zhejiang Tianneng New Materials Co. Ltd.Huzhou313103China

The recycling of cathode materials from spent lithium-ion battery has attracted extensive attention,but few research have focused on spent blended cathode *** reality,the blended materials of lithium iron phosphate and ternary are widely used in electric vehicles,so it is critical to design an effective recycling *** this study,an efficient method for recovering Li and Fe from the blended cathode materials of spent LiFePO_(4)and LiNi_(x)Co_(y)Mn_(1-x-y)O_(2)batteries is ***,87%A1 was removed by alkali ***,91.65%Li,72.08%Ni,64.6%Co and 71.66%Mn were further separated by selective leaching with H_(2)SO_(4)and H_(2)O_(2).Li,Ni,Co and Mn in solution were recovered in the form of Li_(2)CO_(3)and hydroxide ***,98.38%Fe was leached from the residue by two stage process,and it is recovered as FePO_(4)·2H_(2)O with a purity of 99.5%by *** and P were present in FePO_(4)·2H_(2)O in amounts of 28.34%and 15.98%,***,the drift and control of various components were discussed,and cost-benefit analysis was used to assess the feasibility of potential application.

关键词： Spent lithium-ion battery Blended cathode materials Recovery Lithium carbonate Iron phosphate

Effect of Fe Addition on Dehydrogenation Performance of Methylcyclohexance over Pt/Al_(2)O_(3)

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China Petroleum Processing & Petrochemical Technology 2024年第1期26卷 12-18页

作者： Tong Fengya Tian Hao Wang Hao miao Changxi song Lei Shanghai Research Institute of Petrochemical Technology SINOPEC Shanghai201208

Catalysts with varying Fe contents were prepared using a sequential impregnation method to investigate the effects of Fe addition on the physicochemical properties of Pt/Al_(2)O_(3) and their performance in methylcyclohexane(MCH)*** results demonstrated that the addition of Fe to Pt/Al_(2)O_(3) enhanced the electron density of Pt and improved catalytic activity,while exhibiting negligible influence on the catalytic selectivity for *** the Fe content was 0.057%,the catalyst exhibited the highest MCH consumption rate,which was approximately two times higher than that of the catalyst without ***,the incorporation of Fe inhibited the formation of coke and reduced the quantity of coke deposits on the catalyst,thereby improving its catalytic ***,Fe shows promise as a prospective secondary element for Pt/Al_(2)O_(3) to enhance the MCH dehydrogenation performance.

关键词： methylcyclohexane dehydrogenation hydrogen storage deactivation Fe addition

Anti-lipid-oxidation effects and edible safety evaluation of the oil extracted by a supercritical CO_(2) process from coix seed fermented by Monascus purpureus

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Food Science and Human Wellness 2023年第4期12卷 1119-1127页

作者： Haiying Zeng Anran Zhu Shengling He Mingjun Wu Muhammad Mazhar Anyan Wen Na Liu Likang Qin song miao Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB)College of Life Sciences/Institute of Agro-bioengineeringGuizhou UniversityGuiyang 550025China School of Liquor and Food Engineering Guizhou UniversityGuiyang 550025China Teagasc Food Research Centre MooreparkFermoyCo.Cork P61 C996Ireland

The physicochemical properties and composition of coix seed oil produced by Monascus purpureus fermentation and supercritical CO_(2)extraction were ***-lipid-oxidation and edible safety were evaluated using a cholesterol-fish oil model,acute oral toxicity assay,and genetic toxicity assay in vitro and in vivo,*** results show that the extraction oil from fermented coix seed(FCS-O)had good physicochemical quality and abundant active components with physiological *** particular,γ-tocotrienol,γ-oryzanol,coixenolide and oleic acid concentrations reached 72.83μg/g,745.96μg/g,9.65 mg/g and 316.58 mg/100 g DW,*** FCS-O exhibited higher antioxidant capability in inhibiting lipid oxidation and *** to the blank control,the concentrations of 7-ketocholestreol and peroxide only were 8.42μg/mL and 16.16 mmol/kg at 168 h of oxidation(P10 g/kg bw,considered actually non-toxic)and no induced mutagenicity,cytotoxicity or *** results serve as a good safety reference for future application of the oil from fermented coix *** development and utilization of this kind of oil will be beneficial as a food,food ingredient,nutritional supplement,or natural food antioxidant to promote good health function.

关键词： Coix seed oil Composition Edible safety Mutagenicity Genotoxicity

铜纤维毡的模板-热还原法制备及二氧化碳电还原性能

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河北科技师范学院学报 2024年第2期38卷 66-72页

作者：张璐沈子千王若珊宋苗兰帅申偲伯河北科技师范学院化学工程学院河北省天然产物活性成分与功能重点实验室河北秦皇岛066600

为了研究人工碳纳米材料模板对铜纤维毡制备及结构的影响,应用模板-热还原法,以3种碳材料为模板,通过优化前驱体中CuCl_(2)·2H_(2)O与模板的质量比尝试制备铜纤维毡。使用X射线衍射仪、扫描电子显微镜和能量色散谱仪分析了试样的结构... 详细信息

为了研究人工碳纳米材料模板对铜纤维毡制备及结构的影响,应用模板-热还原法,以3种碳材料为模板,通过优化前驱体中CuCl_(2)·2H_(2)O与模板的质量比尝试制备铜纤维毡。使用X射线衍射仪、扫描电子显微镜和能量色散谱仪分析了试样的结构和化学组成。结果表明,石墨烯可发挥模板作用,得到橙红色厘米级铜纤维毡,铜纤维毡中铜纤维的直径约5μm,且铜纤维表面分布亚微米级台阶。将铜纤维毡制成自支撑电极,应用在线气相色谱仪和核磁共振波谱仪评价了铜纤维毡的CO_(2)电化学还原性能。相对于泡沫铜,铜纤维毡作为CO_(2)电还原反应的催化剂,展现了对甲酸生成较高的选择性。

关键词：铜纤维毡模板-热还原法石墨烯二氧化碳电还原性能

Growth inhibition induced by short hairpin RNA to silence survivin gene in human pancreatic cancer cells

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Hepatobiliary & Pancreatic Diseases International 2010年第1期9卷 69-77页

作者： Shen, Yong-Mei Yang, Xiao-Chun song, miao-Li Qin, Chen-Hao Yang, Chen Sun, Yi-Hui Soochow Univ Dept MRI Affiliated Hosp 2 Suzhou 215004 Peoples R China Soochow Univ Dept Gen Surg Affiliated Hosp 2 Suzhou 215004 Peoples R China Municipal Hosp Suzhou Radioimmunoassay Ctr Suzhou 215002 Peoples R China Soochow Univ Radioimmunoassay Ctr Affiliated Hosp 2 Suzhou 215004 Peoples R China Soochow Univ Clin Lab Affiliated Hosp 2 Suzhou 215004 Peoples R China

BACKGROUND: Survivin is known to be overexpressed in various human malignancies, including pancreatic cancer, and mediates cancer cell proliferation and tumor growth, so the regulation of this molecule could be a new strategy for treating pancreatic cancer. In this study, short hairpin RNAs (shRNAs) specific to survivin were introduced into human pancreatic cancer Patu8988 cells to investigate the inhibitory effects on survivin expression and cell proliferation in vitro and in vivo. METHODS: Three kinds of shRNA specific to the survivin gene were designed and cloned into eukaryotic expression plasmid pGenesil-1 vector. Subsequently the recombinant plasmids were transfected into human pancreatic cancer Patu8988 cells with lipfectamine (TM) 2000 reagent. The mRNA and protein expressions of survivin in the transiently transfected Patu8988 cells were determined by RT-PCR, flow cytometry, and Western blotting analysis. The proliferation inhibition rates of stably transfected Patu8988 cells were determined by MTT assay. The antitumor activities of the three kinds of survivin-shRNA plasmids were evaluated in BALB/c nude mice inoculated with Patu8988 cells and bearing human pancreatic cancer. RESULTS: The three survivin-shRNA plasmids named pGenesil-1-survivin-1, pGenesil-1-survivin-2 and pGenesil-1-survivin-1+2 (with double interfering RNA sites) were successfully constructed, and were confirmed by restriction enzyme cutting and sequencing. At 48 hours after transfection, the expression of survivin mRNA and protein was inhibited in Patu8988 cells transfected with pGenesil-1-survivin-1, pGenesil-1-survivin-2, and pGenesil-1-survivin-1+2 when compared with that of either pGenesil-1-NC (with scrambled small interfering RNA) transfected cells or control cells (P<0.05). The MTT results showed that the proliferation rates of Patu8988 cells stably transfected with survivin-shRNA plasmids were reduced when compared with that of either pGenesil-1-NC transfected cells or control cel

关键词： pancreatic neoplasms short hairpin RNA survivin pGenesil-1 vector

An all-in-one FeO_(x)-rGO sponge fabricated by solid-phase microwave thermal shock for water evaporation and purification

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Journal of Environmental Sciences 2024年第4期138卷 671-683页

作者： Youkun Sun Xiuwen Zhao Xueling song Jinchen Fan Junhe Yang Yingchun miao Shuning Xiao School of Materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China College of Chemistry and Environmental Science Qujing Normal UniversityQujing 655011China Prytula Igor Collaborate Innovation Center for Diamond Shanghai Jian Qiao UniversityShanghai 201306China

Developing high-efficiency photothermal seawater desalination devices is of significant importance in addressing the shortage of *** much effort made into photothermal materials,there is an urgent need to design a rapidly synthesized photothermal evaporator for the comprehensive purification of complex ***,we report on all-in-one FeOx-rGO photothermal sponges synthesized via solid-phase microwave thermal *** narrow band gap of the semiconductor material Fe_(3)O_(4) greatly reduces the recombination of electron-hole pairs,enhancing non-radiative relaxation light *** abundantπorbitals in rGO promote electron excitation and thermal vibration between the *** of the surface hydrophilicity and hydrophobicity promotes salt resistance while simultaneously achieving the purification of various complex polluted *** optimized GFM-3 sponge exhibitedan enhanced photothermal conversion rate of 97.3% and a water evaporation rate of 2.04 kg/(m^(2)·hr),showing promising synergistic water purification *** findings provide a highly efficient photothermal sponge for practical applicationsof seawater desalination and purification,as well as develop a super-rapid processing methodology for evaporation devices.

关键词： Desalination Photothermal device Microwave synthesis Water purification FeO_(x)-rGO

高尿酸血症对雌性大鼠生殖功能的影响及其机制

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精准医学杂志 2024年第3期39卷 213-217页

作者：龚雪琳邹通宋淼刘呈杨张清孙钰邢士超青岛大学基础医学院病原生物学系山东青岛266071 青岛大学附属青岛妇女儿童医院

目的探讨高尿酸血症(HUA)对雌性大鼠生殖功能的影响及其机制。方法雌性Wistar大鼠18只,随机分为3组,每组各6只。高尿酸组(HUA组)和高尿酸恢复组(HR组)大鼠每天灌胃50 mg/kg尿酸及腹腔注射250 mg/kg氧嗪酸钾,至第9周时,HR组大鼠改用等体... 详细信息

目的探讨高尿酸血症(HUA)对雌性大鼠生殖功能的影响及其机制。方法雌性Wistar大鼠18只,随机分为3组,每组各6只。高尿酸组(HUA组)和高尿酸恢复组(HR组)大鼠每天灌胃50 mg/kg尿酸及腹腔注射250 mg/kg氧嗪酸钾,至第9周时,HR组大鼠改用等体积生理盐水进行灌胃和腹腔注射,HUA组不变,再继续处理7周;对照组(N组)大鼠给予相同体积的生理盐水灌胃及腹腔注射,持续16周。第16周时,测量三组大鼠体质量和子宫、卵巢质量,并计算子宫、卵巢系数;苏木精-伊红染色法观察3组大鼠子宫、卵巢组织的病理变化;酶联免疫吸附试验法检测3组大鼠血清中促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)含量;使用阴道脱落细胞涂片法观察3组大鼠动情周期;采用比色法检测3组大鼠子宫组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)水平;采用免疫荧光法检测3组大鼠子宫组织中肿瘤坏死因子α(TNF-α)的荧光强度。结果与N组大鼠相比,HUA组大鼠子宫系数降低、卵泡数量减少、子宫内膜厚度变薄,动情间期缩短,血清FSH水平增加,子宫组织中SOD、GSH水平降低,而MDA含量增加(F=7.80~111.40,t=3.95~12.37,P<0.05),子宫组织中TNF-α荧光强度增高;与HUA组大鼠相比,HR组卵泡数量显著增多、子宫内膜显著变厚,血清中FSH水平增高,子宫组织中SOD、GSH水平显著增高,而MDA水平显著降低(t=3.40~11.56,P<0.05),子宫组织中TNF-α荧光强度降低。结论HUA可致雌性大鼠生殖功能下降,其可能机制与大鼠血清尿酸水平升高,致性激素分泌紊乱、机体氧化应激水平增高,进而诱导炎症反应发生有关。

关键词：高尿酸血症动情期性腺甾类激素细胞因子类炎症氧化性应激女(雌)性大鼠,Wistar

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Tetraphenylethylene dicycle helicates: circularly polarized luminescence and chiral recognition based on emission wavelength change

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Science China Chemistry 2024年第11期67卷 3817-3824页

作者： Zhi-Rong Xu miao-Li Gao Ming Hu Wei Yu Yan-song Zheng Key Laboratory of Material Chemistry for Energy Conversion and Storage Ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Chiral tetraphenylethylene(TPE) dicycle tetraaldehyde and TPE dicycle tetraacids bearing optically pure groups on the cycles were designed and synthesized. Due to the propeller-like conformation immobilization of TPE unit, this new class of TPE dicycle was resolved into M-and P-enantiomers, which could emit strong circular polarized luminescence(CPL). Interestingly,these TPE helicates displayed exceptional ability of molecule recognition. While the TPE dicycle tetraaldehyde could detect the microscale water in anhydrous tetrahydrofuran, the chiral TPE dicycle tetraacid could display different fluorescent color after interacting with two enantiomers of a wide variety of chiral amines including monoamines, diamines, and aminols, which could be applied to chiral recognition of these chiral amines. Furthermore, the emission wavelength of TPE dicycle tetraacid was found to change linearly with the enantiomer purity of chiral amine from enantiomer excess(ee) percent-100% to +100%, for the first time showing the potential for quantitative chiral analysis of chiral amines based on emission wavelength change. The emission wavelength was affected less by environmental factors than fluorescence intensity, which would enable the chiral analysis based on wavelength change with higher accuracy and repeatability.

关键词： TPE dicycle helicate CPL chiral recognition enantiomer excess analysis emission wavelength change