检索结果-南通市图书馆

The FMRP/ICAM5 mRNA Interaction Uncovers a New Mechanism of Modulating Dendritic Spine Development and Cognitive Behaviors in Fragile X Syndrome

中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Yang-Wuyue Liu mi Zhou Li Zhao Teng Yang Xu Tan Shan Chen Shuang-Shuang Dai Department of Biochemistry and Molecular Biology College of Basic Medical Sciences Army Military Medical University (Third Military Medical University)

Lip polysaccharide(LPS) is an important pathogenic stimulator to activate neutrophils. Metabotropic glutamate receptor 5(mGluR5) is highly expressed in central nervous system that modulates glutamate release and circuits activity. However, the roles and functions of mGluR5 in peripheral systems especially on neutrophils are poorly understood. Here we found that LPS induced both mRNA and protein expressions of mGluR5 in neutrophils. Activation of mGluR5 promotes p65 nuclear translocation in PKC-dependent manner. The ability of cytokines releasing and migration toward LPS treatment in mGluR5 ko neutrophils was dramatically impaired compared with WT neutrophils. Furthermore, LPS induced mGluR5 expression by enhancing p65 binding to mGluR5 upstream promoter element(-779 to-113), which was confirmed by Luciferace and CHIP experiments. These findings demonstrate that induction of mGluR5 by LPS is vital for neutrophils activation, which unveils the inducible functionality of mGluR5 in neutrophils.

关键词： LPS neutrophil,p65 mGluR5

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The FMRP/ICAM5 mRNA Interaction Uncovers a New Mechanism of ...

RFC2 may contribute to the pathogenicity of Williams syndrome revealed in a zebrafish model

中国神经科学学会第十二届全国学术会议

作者： Ya-Ping Pei Yan Zeng Shi-jing Yuan mi Tian Lang Xu Qiong Zhu ming-Gao Zhao Brain and Cognitive Function Laboratory Wuhan University of Science and Technology

Objective Fragile X syndrome（FXS） is the most common cause of intellectual disability in males and the most common single gene cause of autism. Dendrite and spine abnormalities have been correlated with impaired cognitive abilities in FXS, where neurons show a high density of long, thin, and immature dendritic spines. But the causes of these malformations are not yet well understood. Intercellular adhesion molecule 5（ICAM5;Telencephalin）, a protein of the Ig superfamily, has been implicated in neuronal and spine development as well as the formation and maintenance of dendritic filopodia. The present study is to examine a possible link for ICAM5 in Fmr1 knockout（ko） mice, the animal model of FXS and to futher investigate the direct interaction of FMRP and ICAM5. Methods Fmr1 knock out（ko）（FVB 129 P2-Pde6 b+Tyrc-ch-Fmr1 tm1 Cgr/J） and control（FVB.129 P2-Pde6 b Tyrc-ch/Ant J）, here-after referred to as wild-type（WT） strain mice were used as the animal model of FXS. Primary mouse neuronal cultures were generated from prefrontal cerebral cortex of WT or Fmr1-ko embryos（E17–E18）. The pattern of postnatal spine development and ICAM5 expression was profiled by Golgi staining and western blot respectively. The interaction between Clstn1 and Icam5 m RNA was identified by RNA binding protein immunoprecipitation and quantitative real-time PCR. The alteration of dendritic spine in neurons was measured by live cell imaging. Results（1） In Fmr1 ko brains and cultured neurons, ICAM5 m RNA translation was enhanced by the loss of FMRP, leading to excessive production of ICAM5 protein and preventing filopodiato-spine transition, but ICAM5 m RNA was less affected.（2） Genetic reduction of ICAM5 by using si RNA ICAM5 normalized the aberrant spine maturation and ameliorated behavior impairments including spatial and fear memory in Fmr1 ko mice.（3） FMRP was immunoprecipitated with ICAM5 m RNA from mouse brain and the binding region in ICAM5 was localized to the two first Ig domains. Conclus

关键词： Fragile X syndrome fragile X mental retardation protein intercellular adhesion molecule-5 dendritic spine interaction

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2024年第12期51卷 1389-1403页

作者： Ji-Won Park Tae-Ik Choi Tae-Yoon Kim Yu-Ri Lee Dilan Wellalage Don Jaya K.George-Abraham Laurie A.Robak Cristina C.Trandafir Pengfei Liu Jill A.Rosenfeld Tae Hyeong Kim Florence Petit Yoo-mi Kim Chong Kun Cheon Yoonsung Lee Cheol-Hee Kim Department of Biology Chungnam National University Department of Pediatrics The University of Texas at Austin Dell Medical School Department of Molecular and Human Genetics Baylor College of Medicine Baylor Genetics Laboratories Department of Pediatrics Kyung Hee University Hospital at Gangdong Univ.Lille CHU LilleClinique de g en etique Guy Fontaine Department of Pediatrics Chungnam National University Sejong Hospital Department of Pediatrics Pusan National University Children’s Hospital Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan Hospital Clinical Research Institute Kyung Hee University Hospital at GangdongSchool of MedicineKyung Hee University

Williams syndrome (WS) is a rare multisystemic disorder caused by recurrent microdeletions on 7q11.23,characterized by intellectual disability,distinctive craniofacial and dental features,and cardiovascular *** studies have explored the roles of individual genes within these microdeletions in contributing to WS ***,we report five patients with WS with 1.4 Mb-1.5 Mb microdeletions that include RFC2,as well as one patient with a 167-kb microdeletion involving RFC2 and six patients with intragenic variants within *** investigate the potential involvement of RFC2 in WS pathogenicity,we generate a rfc2 knockout (ko) zebrafish using CRISPR-Cas9 ***,we generate a ko zebrafish of its paralog gene,rfc5,to better understand the functions of these RFC genes in development and *** rfc2 and rfc5 ko zebrafish exhibit similar phenotypes reminiscent of WS,including small head and brain,jaw and dental defects,and vascular ***-seq analysis reveals that genes associated with neural cell survival and differentiation are specifically affected in rfc2 ko *** addition,heterozygous rfc2 ko adult zebrafish demonstrate an anxiety-like behavior with increased social *** results suggest that RFC2 may contribute to the pathogenicity of WS,as evidenced by the zebrafish model.

关键词： Williams syndrome RFC2 RFC5 Zebrafish Knockout CRISPR-Cas9

MYB46-Mediated Transcriptional Regulation of Secondary Wall Biosynthesis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2012年第5期5卷 961-963页

作者： Jae-Heung ko Won-Chan Kim Joo-Yeol Kim Sung-Ju Ahn Kyung-Hwan Han Department of Plant and Environmental New Resources Kyung Hee University Yongin Korea Bioenergy Center Kyung Hee University Yongin Korea Department of Horticulture and Department of Forestry Michigan State University East Lansing MI 48824-1222 USA DOE-Great Lakes Bioenergy Research Center Michigan State University East Lansing MI 48824-1222 USA Department of Bioenergy Science and Technology (WCU) Chonnam National University 333 Yongbongro Buk-gu Gwangju 500-757 Korea

Formation of secondary wall requires coordinated transcrip- tional regulation of the genes involved in the biosynthesis of major secondary wall components （e.g. cellulose, hemicellu- lose, and lignin）. Even though many aspects of plant biology have been extensively elucidated using various model spe- cies, our current understanding of secondary wall formation is limited.

关键词： Biosynthesis Regulation Wall

陕西省小农户作物生产的减肥潜力及经济效益评价

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国农业科学 2021年第20期54卷 4370-4384页

作者：米晓田石磊何刚王朝辉西北农林科技大学资源环境学院/农业农村部西北植物营养与农业环境重点实验室陕西省耕地质量与农业环境保护工作站

【目的】小农户在农业生产中投入的生产资料普遍过高,随种植业结构演变,肥料投入也势必会发生变化。明确小农户经营模式下粮食、油料和经济作物的施肥现状和经济效益,能为小农户作物生产的养分管理、肥料减施和经济效益提高提供参考。... 详细信息

【目的】小农户在农业生产中投入的生产资料普遍过高,随种植业结构演变,肥料投入也势必会发生变化。明确小农户经营模式下粮食、油料和经济作物的施肥现状和经济效益,能为小农户作物生产的养分管理、肥料减施和经济效益提高提供参考。【方法】2018年对陕西省的主要农作物生产情况进行问卷调查,以粮食(小麦、玉米、水稻、谷子、马铃薯)、油料(油菜、大豆)、经济(苹果、西红柿、烤烟)作物为研究对象,共获得1 709份调研问卷。基于此调研结果,以产量水平为分类依据,分析小农户的施肥现状、减肥潜力和经济效益。【结果】小麦、玉米、水稻、谷子和马铃薯的平均产量分别为4.6、7.3、8.3、3.7和19.8 t·hm-2,氮肥用量分别为177、247、186、255和209 kg N·hm-2,磷肥用量分别为102、103、88、142和125 kg P2O5·hm-2,钾肥用量分别为37、47、64、53和110 kg K2O·hm-2。油菜和大豆的平均产量分别为2.4和2.7t·hm-2,氮肥用量分别为156和99 kg N·hm-2,磷肥用量分别为80和63 kg P2O5·hm-2,钾肥用量分别为56和26 kg K2O·hm-2。苹果、西红柿和烤烟的平均产量分别为23.8、93.5和2.7 t·hm-2,氮肥用量分别为731、471和108 kg N·hm-2,磷肥用量分别为482、387和118 kg P2O5·hm-2,钾肥用量分别为535、447和132 kg K2O·hm-2。对大多数作物,氮磷肥普遍过量施用、钾肥施用过量与不足并存。粮食作物氮磷钾减肥潜力分别为28%—60%、52%—66%和11%—51%。油料作物油菜可减少氮磷钾肥料用量33%、37%和46%,然而大豆需增加磷钾肥用量11%、28%。苹果和西红柿等高产经济作物的氮磷钾减肥潜力分别为41%—67%、65%—70%和49%—64%。烤烟生产需减少磷肥53%,然而中、高产农户氮肥施用不足,分别需增施22%、11%。复合肥和单质肥是作物氮素的主要来源,复合肥是作物磷钾的主要来源。氮以基施为主、追施为辅,磷钾肥几乎全部作为基肥一次施用,鲜有追施。粮食、油料和经济作物的平均净经济效益分别0.44×10~4—1.63×10~4、0.75×10~4—0.84×10~4和4.19×10~4—15.05×10~4元/hm~2,肥料占总投入比例分别为31%—52%、57%—59%和48%—65%。苹果、西红柿等高产经济作物的净经济效益远高于粮食和油料作物,成为小农户经营者的首选。然而,在经济作物生产中过量的肥料施用产生了巨大的环境风险。【结论】低中产组小农户是化肥减量和收益提升的主要对象,苹果、西红柿等经济作物的净经济效益高,但单位面积施肥量大、减肥潜力大。值得注意的是,农作物生产中也存在施肥不足的现象,例如大豆和烤烟。由此可见,小农户作物生产的肥料管理变异大,提高小农户的肥料管理水平有利于增加作物产量和经济效益、助力农业可持续发展。

关键词：农户行为作物类型养分管理产量经济效益陕西省

A molecular framework for seasonal growth-dormancy regulation in perennial plants

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2014年第1期1卷 22-30页

作者： Donghwan Shim Jae-Heung ko Won-Chan Kim Qijun Wang Daniel E Keathley Kyung-Hwan Han Schatz Center for Tree Molecular Genetics Pennsylvania State UniversityUniversity ParkPA16802USA Department of Plant&Environmental New Resources College of Life ScienceKyung Hee UniversityYongin-siGyeonggi-do 446-701Republic of Korea Department of Horticulture Michigan State UniversityEast LansingMI 48824USA Department of Forestry Michigan State UniversityEast LansingMI 48824USA konjac Research Center College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China

The timing of the onset and release of dormancy impacts the survival,productivity and spatial distribution of temperate horticultural and forestry perennials and is mediated by at least three main regulatory programs involving signal perception and processing by phytochromes(PHYs)and PHY-interacting transcription factors(PIFs).PIF4 functions as a key regulator of plant growth in response to both external and internal *** poplar,the expression of PIF4 and PIF3-LIKE1 is upregulated in response to short days,while PHYA and PHYB are not regulated at the transcriptional *** of light and environmental signals is achieved by gating the expression and transcriptional activity of *** this annual cycle,auxin promotes the degradation of Aux/IAA transcriptional repressors through the SKP–Cullin-F–boxTIR1 complex,relieving the repression of auxin-responsive genes by allowing auxin response factors(ARFs)to activate the transcription of auxin-responsive genes involved in growth *** of transcriptome changes during dormancy transitions have identified MADS-box transcription factors associated with endodormancy *** studies show that poplar dormancy-associated MADS-box(DAM)genes PtMADS7 and PtMADS21 are differentially regulated during the growth-dormancy *** may be regulated by internal factors,which are specifically localized in ***7/PtMADS21 may function as an internal regulator in *** control of flowering time shares certain regulatory hierarchies with control of the dormancy/growth ***,the particularities of different stages of the dormancy/growth cycle warrant comprehensive approaches to identify the causative genes for the entire cycle.A growing body of knowledge also indicates epigenetic regulation plays a role in these processes in perennial horticultural and forestry *** increased knowledge contributes to better understanding of the dormancy process and consequently to pr

关键词： seasonal knowledge temperate

Ascorbic Acid Attenuates Acute Ethanol-Induced Liver Injury in SMP30 Knockout mice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2017年第12期8卷 1114-1126页

作者： Eun-mi Cho H. M. Arif Ullah Ahmed K. Elfadl Myung-Jin Chung Soong-koo Kim Yong Deuk Kim Eun-Joo Lee Kyu-Shik Jeong College of Veterinary Medicine Kyungpook National University Daegu Republic of Korea

Ascorbic acid (AA) is recognized as a free radical scavenger that protects cells from oxidative stress-induced damage. However, no studies have investigated the role of AA in acute alcoholic liver disease using senescence marker protein-30 (SMP30) knockout (ko) mice. SMP30 is a novel 34-kDa protein involved in AA biosynthesis. The present study aimed to elucidate the physiological functions of AA in acute ethanol-induced liver injury using SMP30 ko mice, which cannot synthesize AA in vivo. After a 4-week experimental period, mice were divided into six groups. The following three groups comprised the ethanol treatment groups: WT-E group (wild-type), KV-E group (AA-supplemented), and KT-E group (AA-deficient). mice were exposed to an acute dose of ethanol (6 g ethanol/kg) administered by gavage once a day for three days. The other three control groups, namely, WT-C, KV-C, and KT-C control groups, received an equal volume of water via oral administration. Analysis of changes in body weight showed that mice in the KT-E group had significant loss of body weight compared to the control, KV-E, and WT-E groups. Behavioral analysis revealed that alcohol exposure significantly increased alcohol sensitivity in the KT-E group, whereas the WT-E, KV-E, and control groups developed ethanol tolerance. Aspartate transaminase (AST) levels in the KT-E group were significantly higher than those in the control, KV-E, and WT-E groups. The number of large and binucleated hepatocytes was significantly higher in the KT-E group than in the KV-E and WT-E groups. In addition, cytochrome P450 2E1 (CYP2E1) was over expressed in the central vein in the KT-E group when compared to the KV-E and WT-E groups. Our current findings indicate that AA supplementation in SMP30 ko mice can alleviate alcohol-induced liver damage by down regulating CYP2E1 expression. These results suggest that reduced CYP2E1 expression is a novel mechanism responsible for AA-induced reduction of ethanol-mediated oxidative str

关键词： Ascorbic Acid Alcohol Liver SMP30

aldh2基因表达对氯胺酮诱导的小鼠膀胱炎症及纤维化的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

广西医科大学学报 2020年第4期37卷 586-592页

作者：奚晓剑米华广西医科大学第一附属医院泌尿外科南宁530021

目的:探究乙醛脱氢酶2(aldh2)基因表达对氯胺酮诱导的小鼠膀胱炎症及纤维化的影响。方法:首先将8周龄ICR雄性野生型(WT)小鼠共20只随机分为氯胺酮(50 mg/kg)组和生理盐水组,分别诱导4周和8周。取出各组小鼠膀胱,运用实时荧光定量聚合酶... 详细信息

目的:探究乙醛脱氢酶2(aldh2)基因表达对氯胺酮诱导的小鼠膀胱炎症及纤维化的影响。方法:首先将8周龄ICR雄性野生型(WT)小鼠共20只随机分为氯胺酮(50 mg/kg)组和生理盐水组,分别诱导4周和8周。取出各组小鼠膀胱,运用实时荧光定量聚合酶链反应(qPCR)法检测各组WT小鼠aldh2 mRNA的表达情况。随后再选取ICR雄性aldh2基因敲除型(ko)与WT型小鼠各20只,分别随机分为生理盐水对照组和氯胺酮诱导组(50 mg/kg)。在第4周和8周时,取出膀胱组织用于检测其炎症和纤维化水平。结果:qPCR显示经氯胺酮处理后的WT小鼠膀胱aldh2 mRNA的相对表达量(1.84±0.24)显著高于生理盐水组(1.02±0.15)(Pko小鼠较WT小鼠体重增加缓慢(Pko炎症细胞因子白细胞介素1β(IL-1β)及6(IL-6)的表达显著增加(Pko小鼠膀胱黏膜屏障消失、炎症细胞浸润、黏膜下层和肌层纤维化增加;蛋白质印迹(Western blot)结果也提示ko小鼠胶原蛋白COL-I的表达量(1.16±0.10)较WT小鼠(0.73±0.07)明显增加(Pko与WT小鼠的炎症和纤维化水平比较,无统计学差异(P>0.05)。结论:aldh2基因可在氯胺酮相关性膀胱炎(KIC)的发生发展中发挥一定的抗炎抗纤维化的保护作用。

关键词：氯胺酮相关性膀胱炎乙醛脱氢酶2 炎症纤维化

维普期刊数据库

二氢杨梅素通过SIRT3调节2型糖尿病小鼠肠上皮屏障功能作用

在线全文

学校读者我要写书评

暂无评论

陆军军医大学学报 2023年第3期45卷 192-201页

作者：岳静周杰荆金金董妞刘明华易龙糜漫天陆军军医大学(第三军医大学)军事预防医学系营养与食品安全研究中心重庆市医学营养研究中心重庆市营养与食品安全重点实验室重庆400038

目的研究二氢杨梅素(dihydromyricetin,DHM)对高脂饮食(high-fat diet,HFD)诱导2型糖尿病小鼠肠上皮屏障功能的作用,并揭示SIRT3在介导DHM改善肠上皮屏障中的作用机制。方法选用7周龄的SPF级雄性野生型(WT)小鼠18只和SIRT3敲除(SIRT3 ko... 详细信息

目的研究二氢杨梅素(dihydromyricetin,DHM)对高脂饮食(high-fat diet,HFD)诱导2型糖尿病小鼠肠上皮屏障功能的作用,并揭示SIRT3在介导DHM改善肠上皮屏障中的作用机制。方法选用7周龄的SPF级雄性野生型(WT)小鼠18只和SIRT3敲除(SIRT3 ko)小鼠30只,分别按随机数字表法分为对照组(NC)、高脂组(HFD)、高脂加二氢杨梅素组(HFD+DHM),定期监测动物体质量、摄食量。干预12周,检测小鼠肠道通透性。小鼠处死后,检测血清生化指标和LPS水平及肠道组织病理学。利用分离的小鼠小肠上皮细胞和Caco-2细胞系,通过Western blot检测紧密连接蛋白(ZO-1、Occludin)和qRT-PCR检测炎症因子(TNF-α、IL-1β、IL-6)表达。进一步利用慢病毒(LV-SIRT3)感染Caco-2细胞敲降SIRT3表达,DHM和LPS干预后检测相关炎症因子和紧密连接蛋白表达。结果HFD诱导WT和SIRT3 ko小鼠体质量明显增加、糖脂代谢异常、肠道通透性增加、肠上皮结构改变(小肠隐窝深度和上皮绒毛长度减少)、肠上皮细胞紧密连接蛋白表达降低和炎症因子表达增加,且与WT小鼠相比,HFD诱导的SIRT3 ko小鼠相关指标改变具有统计学意义(Pko小鼠中不明显,差异无统计学意义(P>0.05)。体外实验发现,在LV-SIRT3感染的Caco-2细胞中,DHM对LPS诱导的炎症因子表达增加和紧密连接蛋白表达降低作用不明显,且差异无统计学意义(P>0.05)。结论SIRT3在DHM增加小肠上皮紧密连接蛋白表达、降低炎症因子表达、维持肠上皮屏障完整性和减少通透性、改善糖脂代谢和延缓T2DM发生中发挥重要作用。

关键词：二氢杨梅素 2型糖尿病肠上皮屏障 SIRT3 紧密连接蛋白

维普期刊数据库