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Visible-light-driven photocatalytic inactivation of bacteriophage f2 by Cu-TiO_2 nanofibers in the presence of humic acid

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Journal of Environmental Sciences 2019年第3期31卷 383-391页

作者： Rong Cheng mi kang Zhi-peng Shen Lei Shi Xiang Zheng School of Environment & Natural Resources Renmin University of China

Pathogenic viruses in drinking water are great threats to public health. Visible-lightdriven photocatalysis is a promising technology for virus inactivation. However, the existing photocatalytic antiviral research studies have mostly been carried out in single-component systems, neglecting the effect of natural organic matter, which exists widely in actual water bodies. In this paper, electrospun Cu-TiO2 nanofibers were prepared as photocatalysts, and their photocatalytic antiviral performance in the presence of humic acid(HA) was comprehensively studied for the first time. The properties of the reaction mixture were measured during the reaction. In addition, the safety, reliability and stability of photocatalytic disinfection in the mixed system were evaluated. The results showed that the virus removal efficiency decreased with the increase of the HA concentration. The type of reaction solution, such as PBS buffer solution or water, did not affect the removal efficiency noticeably. Under acidic conditions, the electrostatic forces between photocatalysts and viruses were strengthened, leading to higher virus removal efficiency. As the reaction time went on, the pH value in the solution increased first and then tended to be stable, the conductivity remained stable, and the dissolved oxygen increased first and then decreased. The safety test showed that the concentration of Cu ions released into the solution was lower than specified by the international standards. No photoreactivation was observed, and the addition of HA significantly reduced the reutilization efficiency of the photocatalysts.

关键词： Photocatalysis Visible light Bacteriophage f2 Humic acid

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Systemic lupus erythematosus in a 15-year-old female with multiple splenic nodules:A case report

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World Journal of Clinical Cases 2024年第12期12卷 2128-2133页

作者： mi Il kang Hyeok Chan Kwon Division of Rheumatology Department of Internal MedicineDankook University College of MedicineCheonan-si 31116South Korea Division of Rheumatology Department of Internal MedicineDankook University HospitalDankook University College of MedicineCheonan-si 31116South Korea

BACKGROUND Systemic lupus erythematosus(SLE)is a chronic inflammatory disease primarily affecting young *** can invade any organ,and various forms of splenic invasion have been *** include splenomegaly and splenic infarction,rupture,and *** study encountered a rare case of splenic involvement,with nodules of various sizes without calcifications or *** SUMMARY A 15-year-old girl presented with arthralgia,weight loss,fever,increased levels of inflammatory markers,and positive antinuclear antibody test *** patient was diagnosed with *** was asymptomatic while taking steroids and *** months after discharge,the patient developed a fever and abdominal *** enteritis was suspected,and abdominopelvic computed tomography(AP-CT)was *** were no specific findings in the gastrointestinal tract,but multiple splenic nodules were *** or hemangioma was considered;however,no specific radiological findings were observed.A biopsy of the spleen was performed to determine the possibility of *** histological findings of the spleen included extensive periarteriolar necrosis with hematoxylin bodies and numerous karyorrhectic *** on the biopsy results,the patient was diagnosed with an SLE flare-up and was maintained on high-dose steroids and *** As disease activity increased,multiple nodules in the spleen that were previously unseen were observed using AP-CT and histologically *** invasion by SLE can appear in multiple nodular forms and ***,physicians should consider these findings when differentiating these nodules from infections and malignancies.

关键词： Multiple nodules Spleen Splenectomy Systemic lupus erythematosus Differential diagnosis Case report

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All-optical attosecond time domain interferometry

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National Science Review 2021年第10期8卷 15-22页

作者： Zhen Yang Wei Cao Yunlong Mo Huiyao Xu kang mi Pengfei Lan Qingbin Zhang Peixiang Lu Wuhan National Laboratory for Optoelectronics and School of Physics Huazhong University of Science and Technology Hubei Key Laboratory of Optical Information and Pattern Recognition Wuhan Institute of Technology CAS Center for Excellence in Ultra-intense Laser Science

Interferometry, a key technique in modern precision measurements, has been used for length measurement in engineering metrology and astronomy. An analogous time-domain interferometric technique would represent a significant complement to spatial domain applications and require the manipulation of interference on extreme time and energy scales. Here, we report an all-optical interferometer using laser-driven high order harmonics as attosecond temporal slits. By controlling the phase of the temporal slits with an external field, a time domain interferometer that preserves both attosecond temporal resolution and hundreds of me V energy resolution is implemented. We apply this exceptional temporal resolution to reconstruct the waveform of an arbitrarily polarized optical pulse, and utilize the provided energy resolution to interrogate the abnormal character of the transition dipole near the Cooper minimum in argon. This novel attosecond interferometry paves the way for high precision measurements in the time-energy domain using all-optical approaches.

关键词： interferometry attosecond all-optical precision measurement

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Human umbilical cord blood-derived mesenchymal stem cells promote regeneration of crush-injured rat sciatic nerves

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Neural Regeneration Research 2012年第26期7卷 2018-2027页

作者： mi-Ae Sung Hun Jong Jung Jung-Woo Lee Jin-Yong Lee kang-mi Pang Sang Bae Yoo Mohammad S. Alrashdan Soung-min Kim Jeong Won Jahng Jong-Ho Lee Department of Craniofacial Structure & Functional Biology School of Dentistry Seoul National University Seoul Republic of Korea Department of Occupation and Environment Postgraduate College of Medicine Konkuk University Choong-ju Republic of Korea Department of Oral & Maxillofacial Surgery School of Dentistry Seoul National University Seoul Republic of Korea Department of Dentistry Oral and Maxillofacial Surgery Ajou University School of Medicine Suwon Republic of Korea Dental Research Institute Seoul National University Seoul Republic of Korea

Several studies have demonstrated that human umbilical cord blood-derived mesenchymal stem cells can promote neural regeneration following brain injury. However, the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells in guiding peripheral nerve regeneration remain poorly understood. This study was designed to investigate the effects of human umbilical cord blood-derived mesenchymal stem cells on neural regeneration using a rat sciatic nerve crush injury model. Human umbilical cord blood-derived mesenchymal stem cells （1 ~ 106） or a PBS control were injected into the crush-injured segment of the sciatic nerve. Four weeks after cell injection, brain-derived neurotrophic factor and tyrosine kinase receptor B mRNA expression at the lesion site was increased in comparison to control. Furthermore, sciatic function index, Fluoro Gold-labeled neuron counts and axon density were also significantly increased when compared with control. Our results indicate that human umbilical cord blood-derived mesenchvmal stem cells promote the functinnal r~.RcJv^rv nf P.n I^h-inillr^4 ~r^i~tit, n^r~e

关键词： human umbilical cord blood-derived mesenchymal stem cells sciatic nerve crush injury FluoroGold stem cells peripheral nerve regeneration regeneration neural regeneration

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Transplantation of mesenchymal stem cells from human umbilical cord versus human umbilical cord blood for peripheral nerve regeneration

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Neural Regeneration Research 2010年第11期5卷 838-845页

作者： kang-mi Pang mi-Ae Sung Mohammad S.Alrashdan Sang Bae Yoo Samir Jabaiti Soung-min Kim Sung-June Kim Myung-Jin Kim Jeong Won Jahng Jong-Ho Lee Department of Oral Maxillofacial Surgery Seoul National University Seoul 110-768 Korea Department of Craniofacial Structure & Functional Biology Seoul National University Seoul 110-768 Korea Dental Research Institute School of Dentistry Seoul National University Seoul 110-768 Korea Department of Plastic and Reconstructive Surgery Faculty of Medicine Jordan University Hospital Amman 11942 Jordan Department of Electrical Engineering School of Electrical and Computer Science Seoul National University Seoul 110-768 Korea

BACKGROUND： Mesenchymal stem cells （MSCs） appear to be a good alternative to Schwann cells in the treatment of peripheral nerve injury. Fetal stem cells, like umbilical cord blood （UCB） and umbilical cord （UC） stem cells, have several advantages over adult stem cells. OBJECTIVE： To assess the effects of UC-derived MSCs （UCMSCs） and UCB-derived MSCs （UCBMSCs） in repair of sciatic nerve defects. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was performed at the laboratory of Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, from July to December 2009. MATERIALS： UCMSCs were provided by the Research Institute of Biotechnology, Dongguk University. UCBMSCs were provided by the Laboratory of Stem Cells and Tumor Biology, College of Veterinary Medicine, Seoul National University. Dulbecco＇s modified Eagle＇s medium （DMEM） was purchased from Gibco-BRL, USA. METHODS： Seven-week-old Sprague-Dawley rats were randomly and evenly divided into three groups： DMEM, UCBMSCs, and UCMSCs. A 10-mm defect in the left sciatic nerve was constructed in all rats. DMEM （15 μL） containing 1×10^6 UCBMSCs or UCMSCs was injected into the gap between nerve stumps, with the surrounding epineurium as a natural conduit. For the DMEM group, simple DMEM was injected. MAIN OUTCOME MEASURES： At 7 weeks after sciatic nerve dissection, dorsal root ganglia neurons were labeled by fluorogold retrograde labeling. At 8 weeks, electrophysiology and histomorphometry were performed. At 2, 4, 6, and 8 weeks after surgery, sciatic nerve function was evaluated using gait analysis. RESULTS： The UCBMSCs group and the UCMSCs group exhibited similar sciatic nerve function and electrophysiological indices, which were better than the DMEM group, as measured by gait analysis （P 〈 0.05）. Fluorogold retrograde labeling of sciatic nerve revealed that the UCBMSCs group demonstrated a higher number of labeled neurons; however, the differences were not significant. His

关键词： peripheral nerve regeneration umbilical cord mesenchymal stem cell umbilical cord blood mesenchymal stem cell axotomy defect stem cells

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Recombinant human fibroblast growth factor-2 promotes nerve regeneration and functional recovery after mental nerve crush injury

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Neural Regeneration Research 2017年第4期12卷 629-636页

作者： Sung Ho Lee Wei-Peng Jin Na Ri Seo kang-mi Pang Bongju Kim Soung-min Kim Jong-Ho Lee Dental Research Institute Department of Oral and Maxillofacial Surgery Seoul National University School of Dentistry Seoul Korea Dental Life Science Research Institute Clinical Translational Research Center for Dental Science Seoul National University Dental Hospital Seoul Korea Dental Research Institute Seoul National University Seoul Korea

Several studies have shown that fibroblast growth factor-2 （FGF2） can directly affect axon regeneration after peripheral nerve damage. In this study, we performed sensory tests and histological analyses to study the effect of recombinant human FGF-2 （rhFGF2） treatment on damaged mental nerves. The mental nerves of 6-week-old male Sprague-Dawley rats were crush-injured for 1 minute and then treated with 10 or 50 μg/mL rhFGF2 or PBS in crush injury area with a mini Osmotic pump. Sensory test using von Frey filaments at 1 week revealed the presence of sensory degeneration based on decreased gap score and increased difference score. However, at 2 weeks, the gap score and difference score were significantly rebounded in the mental nerve crush group treated with 10 μg/mL rhFGF2. Interestingly, treatment with 10 μg/mL rhFGF had a more obviously positive effect on the gap score than treatment with 50 μg/mL rhFGF2. In addition, retrograde neuronal tracing with Dil revealed a significant increase in nerve regeneration in the trigeminal ganglion at 2 and 4 weeks in the rhFGF2 groups （10 μg/mL and 50 μg/mL） than in the PBS group. The 10 μg/mL rhFGF2 group also showed an obviously robust regeneration in axon density in the mental nerve at 4 weeks. Our results demonstrate that 10 μg/mL rhFGF induces mental nerve regeneration and sensory recovery after mental nerve crush injury.

关键词： nerve regeneration mental nerve fibroblast growth factor crush injury sensory neuron functionalrecovery neural regeneration

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Inhibition of histone deacetylase 2 mitigates profibrotic TGF-β1 responses in fibroblasts derived from Peyronie＇s plaque

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Asian Journal of Andrology 2013年第5期15卷 640-645页

作者： Ji-Kan Ryu Woo-Jean Kim min-Ji Choi Jin-mi Park kang-Moon Song mi-Hye Kwon Nando-Dulal Das Ki-Dong Kwon Dulguun Batbold Guo-Nan Yin Jun-Kyu Suh National Research Center for Sexual Medicine and Department of Urology Inha University School of Medicine Incheon 400-711 Korea

Epigenetic modifications, such as histone acetylation/deacetylation, have been shown to play a role in the pathogenesis of fibrotic disease. Peyronie＇s disease （PD） is a localized fibrotic process of the tunica albuginea, which leads to penile deformity. This study was undertaken to determine the anti-fibrotic effect of small interfering RNA （siRNA）-mediated silencing of histone deacetylase 2 （HDAC2） in primary fibroblasts derived from human PD plaque. PD fibroblasts were pre-treated with HDAC2 siRNA and then stimulated with transforming growth factor-p1 （TGF-β1）. Protein was extracted from treated fibroblasts for Western blotting and the membranes were probed with antibody to phospho-Smad2/Smad2, phospho-Smad3/Smad3, smooth muscle α-actin and extracellular matrix proteins, including plasminogen activator inhibitor-β 1, fibronectin, collagen I and collagen IV. We also performed immunocytochemistry to detect the expression of extracellular matrix proteins and to examine the effect of HDAC2 siRNA on the TGF-β1-induced nuclear translocation of Smad2/3 in fibroblasts. Knockdown of HDAC2 in PD fibroblasts abrogated TGF-β1-induced extracellular matrix production by blocking TGF-β1-induced phosphorylation and nuclear translocation of Smad2 and Smad3, and by inhibiting TGF-β1-induced transdifferentiation of fibroblasts into myofibroblasts. Decoding the individual function of the HDAC isoforms by use of siRNA technology, preferably siRNA for HDAC2, may lead to the development of specific and safe epigenetic therapies for PD.

关键词： cell culture fibrosis histone deacetylase Peyronie's disease transforming growth factor-beta

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Shape-controlled Pt nanocubes directly grown on carbon supports and their electrocatalytic activity toward methanol oxidation

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Science Bulletin 2017年第13期62卷 943-949页

作者： Hee Jin Kim Bibi Ruqia mi Sung kang Su Bin Lim Ran Choi Ki min Nam Won Seok Seo Gaehang Lee Sang-Il Choi Department of Chemistry and Green-Nano Materials Research Center Kyungpook National University Department of Chemistry KAIST Department of Chemistry Mokpo National University Department of Chemistry Sogang University Korea Basic Science Institute(KBSI) and University of Science and Technology

Synthesis of shape-controlled Pt nanocrystals is substantial and important for enhancing chemical and electrochemical ***,the removal of capping agents,shape-controlling chemicals,on Pt surfaces is essential prior to conducting the catalytic *** we report a facile one-pot synthesis of Pt nanocubes directly grown on carbon supports(Pt nanocubes/C) with modulating the kinetic reaction factors for shaping the nanocrystals,but without adding any capping agents for preserving the clean Pt ***-dispersed Pt nanocubes/C shows enhanced activity and long-term stability toward methanol oxidation reaction compared to the commercial Pt/C catalyst.

关键词： Platinum Nanocube Shape-control Kinetic product Methanol oxidation

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Magnifying endoscopy of gastric epithelial dysplasia based on the morphologic characteristics

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World Journal of Gastroenterology 2014年第42期20卷 15771-15779页

作者： Hwa mi kang Gwang Ha Kim Do Youn Park Hong Ryeol Cheong Dong Hoon Baek Bong Eun Lee Geun Am Song Department of Internal Medicine Busan Adeventist Hospital Department of Internal Medicine Pusan National University School of Medicine Department of Pathology Pusan National University School of Medicine

AIM: To investigate the difference in magnifying endoscopic findings of gastric epithelial dysplasias (GEDs) according to the morphologic characteristics.

关键词： Stomach Dysplasia Narrow band imaging Magnifying endoscopy Mucin

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无灌溉造林树种耗水特性的研究

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节水灌溉 2022年第12期 110-118页

作者：谢发兵米扛扛宋于洋新疆玛纳斯县林业和草原技术推广中心新疆玛纳斯832200 石河子大学农学院新疆石河子832061

采用无灌溉造林的方法,依据水量平衡技术与Penman-Monteith方程,研究干旱地区造林树种沙棘、梭梭、紫穗槐和锦鸡儿的蒸腾耗水规律、林木需水量、林木系数及林木水分亏缺量。结果如下:4种灌木在生长期内的蒸腾速率皆为双峰型,沙棘、锦鸡... 详细信息

采用无灌溉造林的方法,依据水量平衡技术与Penman-Monteith方程,研究干旱地区造林树种沙棘、梭梭、紫穗槐和锦鸡儿的蒸腾耗水规律、林木需水量、林木系数及林木水分亏缺量。结果如下:4种灌木在生长期内的蒸腾速率皆为双峰型,沙棘、锦鸡儿和紫穗槐的峰值出现时间一致。梭梭的峰值出现在10∶30和16∶30左右。各树种阳坡的蒸腾速率>阴坡的蒸腾速率。4种灌木中紫穗槐的耗水量最大,阳坡与阴坡的耗水量分别为173.4 mm和134.86 mm,梭梭的耗水量最小。干旱地区各树种生长期内的林木系数有所不同,5月份的阴坡梭梭林木系数最小为0.16,7月份阳坡紫穗槐林木系数最大为1.33。紫穗槐的水分亏缺量最大、沙棘次之,梭梭水分亏缺最小。所以梭梭为最适树种,紫穗槐为干旱地区较难生存、生长的树种。

关键词：无灌溉造林蒸腾速率林木耗水量林木需水量林木水分亏缺量

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