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Development of a functional chocolate using gamma-amino butyric acid producer Lacticaseibacillus rhamnosus NRRL B-442

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Food Bioscience 2022年第3期47卷 700-706页

作者： merve ozer Burcu Ozturk Ali Adnan Hayaloglu Sebnem Tellioglu Harsa İzmir Institute of Technology Faculty of EngineeringDepartment of Food EngineeringGulbahçe Campus35430UrlaİzmirTurkey İnönü University Faculty of EngineeringDepartment of Food Engineering44280MalatyaTurkey

The human microbiota is influenced by the immune and nervous systems of the ***-aminobutyric acid (GABA) is known as bioactive compound and it has important physiological functions,such as anti-hypertensive and antidepressant *** acid bacteria (LAB),especially Lactobacillus species are known as the most important GABA producers because of the food-grade *** purpose of this study is to develop a functional chocolate using microencapsulated GABA producer Lacticaseibacillus rhamnosus NRRL B-442 strain for patients having an anxiety ***-in-oil emulsion technique was conducted for microencapsulation using whey-pullulan *** and free *** cell counts were 6.75 and 7.20 log CFU/g in chocolates,respectively,at the end of 60 *** simulated in vitro digestion analysis,survival rate of microencapsulated bacteria in chocolate samples was found at higher percentage (87%) than free bacteria (75%).Furthermore,microencapsulated *** did not affect the physical,chemical,and sensory properties of ***,*** with the highest GABA producing capability may provide insight for an anxiety disorder patient,since this strain has been thought as having a therapeutic effect.A new functional food model was developed for “GutBrain Axis” phenomena since the chocolate could be accepted as a good carrier for GABA producer bacteria.

关键词： Microencapsulation Anxiety disorder Functional food Simulated digestion

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The in-vivo assessment of Turkish propolis and its nano form on testicular damage induced by cisplatin

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Journal of Integrative Medicine 2021年第5期19卷 451-459页

作者： Pinar Tatli Seven Ismail Seven Selcan Karakus Seda Iflazoglu Mutlu Seyma ozer Kaya Gozde Arkali merve Ilgar Ezgi Tan Yesim Muge Sahin Deniz Ismik Ayben Kilislioglu Department of Animal Nutrition and Nutritional Diseases Faculty of Veterinary MedicineFirat UniversityElazig23119Turkey Department of Plant and Animal Production Vocation School of SivriceFtrat UniversityElazig 23119Turkey Department of Chemistry Faculty of EngineeringIstanbul University-CerrahpasaIstanbul 34320Turkey Department of Reproduction and Artificial Insemination Faculty of Veterinary MedicineFirat UniversityElazig 231J9Turkey Department of Physiology Faculty of Veterinary MedicineFirat UniversityElazig 23119Turkey Istanbul Arel University ArelPOTKAM(Polymer Technologies and Composite Application and Research Center)Istanbul 34537Turkey Department of Biomedical Engineering Faculty of Engineering and ArchitectureIstanbul Arel UniversityIstanbul 34537Turkey Department of Electrical Electronics Engineering Faculty of Engineering and Natural SciencesKadir Has UniversityIstanbul 34083Turkey

Objective: Chemotherapeutic drugs, such as cisplatin(CP), which are associated with oxidative stress and apoptosis, may adversely affect the reproductive system. This study tests whether administration of propolis and nano-propolis(NP) can alleviate oxidative stress and apoptosis in rats with testicular damage induced by ***: In this study, polymeric nanoparticles including propolis were synthesized with a green sonication method and characterized using Fourier transform-infrared spectroscopy, Brunauer-EmmettTeller, and wet scanning transmission electron microscopy techniques. In total, 56 rats were divided into the following seven groups: control, CP, propolis, NP-10, CP + propolis, CP + NP-10, and CP + ***(100 mg/kg), NP-10(10 mg/kg), and NP-30(30 mg/kg) treatments were administered by gavage daily for 21 d, and CP(3 mg/kg) was administered intraperitoneally in a single dose. After the experiment,oxidative stress parameters, namely, malondialdehyde(MDA), glutathione(GSH), glutathione peroxidase(GPx), and catalase(CAT), and apoptotic pathways including B cell leukemia/lymphoma-2 protein(Bcl-2)and Bcl-2-associated X protein(Bax) were measured in testicular tissues. Furthermore, sperm quality and weights of the testis, epididymis, right cauda epididymis, seminal vesicles and prostate were ***: Propolis and NP(especially NP-30) were able to preserve oxidative balance(decreased MDA levels and increased GSH, CAT, and GPx activities) and activate apoptotic pathways(decreased Bax and increased Bcl-2) in the testes of CP-treated rats. Sperm motility in the control, CP, and CP + NP-30 groups were 60%, 48.75%, and 78%, respectively(P<0.001). Especially, NP-30 application completely corrected the deterioration in sperm features induced by ***: The results show that propolis and NP treatments mitigated the side effects of CP on spermatogenic activity, antioxidant situation, and apoptosis in rats.

关键词： Cisplatin Nano-propolis Reproductive system Testicular damage

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