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Computer-aided sperm analysis： a useful tool to evaluate patient＇s response to varicocelectomy

Asian Journal of Andrology 2017年第4期19卷 449-452页

作者： Julia I Ariagno Gabriela R Mendeluk Maria J Furlan M Sardi P Chenlo Susana M Curi mercedes n pugliese Herberto E Repetto Mariano Cohen Clinical Biochemistry Department Male Fertility Laboratory Faculty of Pharmacy and Biochemetry University of Buenos Aires Argentina Urology Department "Jose de San Martin" Clinical Hospital University of Buenos Aires Buenos Aires Argentina (CP1120)

Preoperative and postoperative sperm parameter values from infertile men with varicocele were analyzed by computer-aided sperm analysis （CASA） to assess if sperm characteristics improved after varicocelectomy. Semen samples of men with proven fertility （n = 38） and men with varicocele-related infertility （n = 61） were also analyzed. Conventional semen analysis was performed according to WHO （2010） criteria and a CASA system was employed to assess kinetic parameters and sperm concentration. Seminal parameters values in the fertile group were very far above from those of the patients, either before or after surgery. no significant improvement in the percentage normal sperm morphology （P = 0. 10）, sperm concentration （P = 0.52）, total sperm count （P = 0.76）, subjective motility （%）（P = 0.97） nor kinematics （P = 0.30） was observed after varicocelectomy when all groups were compared. neither was significant improvement found in percentage normal sperm morphology （P = 0.91）, sperm concentration （P =0. 10）, total sperm count （P = 0.89） or percentage motility （P = 0.77） after varicocelectomy in paired comparisons of preoperative and postoperative data. Analysis of paired samples revealed that the total sperm count （P = 0.01） and most sperm kinetic parameters： curvilinear velocity （P = 0.002）, straight-line velocity （P = 0.0004）, average path velocity （P = 0.0005）, linearity （P = 0.02）, and wobble （P = 0.006） improved after surgery. CASA offers the potential for accurate quantitative assessment of each patient＇s response to varicocelectomy.

关键词： computer-assisted semen analysis male infertility semen analysis varicocele varicocelectomy

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Our experience in sperm morphology assessment

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Asian Journal of Andrology 2011年第2期13卷 201-202页

作者： Julia Irene Ariagno Susana mercedes Curi Patricia Chenlo Herberto Ernesto Hector Repetto mercedes norma pugliese Luis Alberto Palaoro Melba Sardi Gabriela Ruth Mendeluk Laboratory of Male Fertility Department of ClinicalBiochemistry INFIBIOC Faculty of Pharmacy andBiochemistry University of Buenos Aires BuenosAires 1120 Argentina

We read with great interest the article recently published by Menkeveld et aL1 as we have a double interest in sperm morphology assessment. The first one as a Clinical Andrology Laboratory （Laboratory of Male Fertility, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina）

关键词： Microbiology Physiology Diseases

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