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Liquid Crystal Based Label-Free Optical Sensors forBiochemical Application

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Photonic Sensors 2024年第2期14卷 57-88页

作者： Jieyuan tang Zhibin LI mengyuan XIE Yunhan LUO Jianhui YU Guojie CHEN Zhe CHEN School of Physics and Optoelectronic Engineering Foshan UniversityFoshan 528225China Guangdong-Hong Kong.Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology Foshan UniversiyFoshan 528225China Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes Jinan UniversityGuangzhou 510632.China Department of Optoelectronic Engineering Jinan UniversityGuangzhou 510632China JiHua Laboratory Foshan 528200China

Biochemical sensors have important applications in biology,chemistry,and ***,many biochemical sensors are hampered by intricate techniques,cumbersome procedures,and the need for *** the past two decades,it has been discovered that liquid crystals can be used to achieve the optical amplification of biological *** modifying recognition molecules,a variety of label-free biochemical sensors can be ***,biochemical sensors based on the amplification of liquid crystals have become one of the most promising *** paper describes in detail the optical sensing principle of liquid crystals,sensing devices,and optical detection ***,the latest research findings are ***,the challenges and future research directions are discussed.

关键词： Optical sensor biochemistry liquid crystal label-free

ZmCYP90D1 regulates maize internode development by modulating brassinosteroid-mediated cell division and growth

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The Crop Journal 2024年第1期12卷 58-67页

作者： Canran Sun Yang Liu Guofang Li Yanle Chen mengyuan Li Ruihua Yang Yongtian Qin Yongqiang Chen Jinpeng Cheng Jihua tang Zhiyuan Fu Key Laboratory of Wheat and Maize Crops Science/Collaborative Innovation Center of Henan Grain Crops/College of Agronomy Henan Agricultural UniversityZhengzhou 450046HenanChina The Shennong Laboratory Zhengzhou 450002HenanChina Hebi Academy of Agricultural Sciences Hebi 458030HenanChina Yunnan tang Jihua Expert Workstation/Zhaotong Academy of Agricultural Sciences Zhaotong 657000YunnanChina

Plant height(PH)is associated with lodging resistance and planting density,which is regulated by a complicated gene *** this study,we identified a spontaneous dwarfing mutation in maize,m30,with decreased internode number and length but increased internode diameter.A candidate gene,ZmCYP90D1,which encodes a member of the cytochrome P450 family,was isolated by map-based ***90D1 was constitutively expressed and showed highest expression in basal internodes,and its protein was targeted to the nucleus.A G-to-A substitution was identified to be the causal mutation,which resulted in a truncated protein in *** of function of ZmCYP90D1 changed expression of hormoneresponsive genes,in particular brassinosteroid(BR)-responsive genes which is mainly involved in cell cycle regulation and cell wall extension and modification in *** concentration of typhasterol(TY),a downstream intermediate of ZmCYP90D1 in the BR pathway,was reduced.A haplotype conferring dwarfing without reducing yield was ***90D1 was inferred to influence plant height and stalk diameter via hormone-mediated cell division and cell growth via the BR pathway.

关键词： Maize ZmCYP90D1 BR biosynthesis Dwarf plant

Spatiotemporal distribution, environmental risk and carbon emission reduction potential of livestock manure in Shaanxi Province, China

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Frontiers of Agricultural Science and Engineering 2024年第3期11卷 381-396页

作者： mengyuan QIAO Jianchun ZHU Ronghua LI Junting PAN Ran XIAO Hailong WANG Kuok Ho Daniel tang Zengqiang ZHANG College of Humanities & Social Development Northwest A&F University College of Natural Resources and Environment Northwest A&F University Key Laboratory of Low-carbon Green Agriculture in Northwestern China Ministry of Agriculture and Rural Affairs Key Laboratory of Non-point Source Pollution of Ministry of Agricultural and Rural Affairs Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Interdisciplinary Research Center for Agriculture Green Development in Yangtze River Basin College of Resources and Environment Southwest University Biochar Engineering Technology Research Center of Guangdong Province School of Environmental and Chemical Engineering Foshan University Department of Environmental Science University of Arizona

Shaanxi is a leading province in animal husbandry（AH） in China. However, the lack of provincial information on the characteristics and utilization potential of livestock manure（LM） hinders crucial management decisions. Therefore, we investigated the spatiotemporal distribution, availability and biogas potential of LM in Shaanxi, and examine the carbon emission reduction potential of *** has been a 1.26-fold increase in LM quantities in Shaanxi over the past 35 years, reaching 4635.6 × 10～4 t by 2021. LM was mainly concentrated in northern Shaanxi and the eastern part of Hanzhong. Cattle and pig manure were the primary sources of LM, with the average LM land-load of 14.57 t·ha-1in 2021. While the overall AH in Shaanxi has not exceeded the environmental capacity, the actual scales of AH in Ankang and Hanzhong have already surpassed the respective environmental capacities, posing a higher risk of N and P pollutions. In 2021, the estimated biogas energy potential of LM was 1.2 × 1011MJ. From 2012 to 2021, the average carbon emission reduction potential in Shaanxi was 22%, with an average potential scale of 10%. The results of this research provide valuable data and policy recommendations for promoting the intensive use of LM and reducing carbon emissions in Shaanxi.

关键词： Livestock manure emission estimation N and P loads biogas potential carbon emission reduction

Fuzzy Comprehensive Evaluation and Prediction of Air Quality in Baoding City

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Agricultural Biotechnology 2018年第3期7卷 110-111,115页

作者： mengyuan tang Degang JI Department of Mathematics School of ScienceHebei Agricultural University

Nowadays,the quality of the air environment is closely related to the health of the ecosystem and the safety of people living. With more and more attention attached to the quality of the air environment,it is imminent to analyze the future development trend of air quality. In this paper,the grey correlation analysis was used to determine the weights of 6 pollution indexes,namely PM10,PM2. 5,SO2,NO2,CO and O3. The fuzzy comprehensive assessment method was applied to determine the air quality eigenvalues H（ 2. 145,1. 926,and 1. 805） of Baoding City in 2014-2016,suggesting that the air quality in Baoding is getting better and better. In addition,the grey forecasting model GM（ 1,1） was used to forecast and test the air quality of Baoding City. The results show that the prediction is good.

关键词： Fuzzy comprehensive evaluation Grey theory GM( 1,1 ) Air quality in Baoding City

复杂环境下黄花菜识别的YOLOv7-MOCA模型

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农业工程学报 2023年第15期39卷 181-188页

作者：靳红杰马顾彧唐梦圆陈婧美张银萍葛学峰南京师范大学计算机与电子信息/人工智能学院南京210023 南京邮电大学集成电路科学与工程学院南京210023

黄花菜是极具营养价值和经济效益的一种农作物,深受人们喜爱。目前黄花菜采摘大都是人工采摘,采摘效率低、人工成本较高,在设计黄花菜自动采摘机器人的过程中,复杂环境下黄花菜的目标识别是实现智能化采摘的核心问题。该研究建立了包含1... 详细信息

黄花菜是极具营养价值和经济效益的一种农作物,深受人们喜爱。目前黄花菜采摘大都是人工采摘,采摘效率低、人工成本较高,在设计黄花菜自动采摘机器人的过程中,复杂环境下黄花菜的目标识别是实现智能化采摘的核心问题。该研究建立了包含12000幅黄花菜样本的数据库,比较了You Only Look Once(YOLOv7)、Faster Region Convolutional Neural Networks(Faster R-CNN)和Single Shot MultiBox Detector(SSD)三种模型的检测效果,提出一种基于改进YOLOv7目标检测算法的复杂环境下黄花菜识别的YOLOv7-MOCA模型,使用MobileOne网络作为主干特征提取网络,构建了一种轻量化网络模型,并在颈部网络中融合Coordinate Attention注意力机制改善对样本的检测效果。试验结果表明,YOLOv7-MOCA模型检测准确率为96.1%,召回率为96.6%,F1值为0.96,权重为10 MB,帧速率为58帧/s。较YOLOv7检测速度提高了26.1%,权重减少了86.7%,该研究所提出的YOLOv7-MOCA模型检测准确率等参数均大幅提升。该模型可以实现黄花菜的快速识别,模型权重小,识别速度快,为黄花菜智能化采摘设备研究提供技术支撑。

关键词：识别智能化模型黄花菜复杂环境 YOLOv7 MobileOne网络注意力机制

计及用户行为决策的电动汽车负荷时空分布建模

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南方电网技术 2023年第10期17卷 170-177页

作者：齐彩娟张泽龙吕干云车彬唐梦媛国网宁夏电力有限公司经济技术研究院银川750001 南京工程学院电力工程学院南京211167

发展电动汽车是实现“双碳”战略的重要手段,开展电动汽车充电特性及充电负荷有序调控研究具有重要意义。因此提出了计及用户行为决策的电动汽车充电负荷时空分布建模方法。首先,通过引入后悔理论模拟用户的充电行为决策。其次,结合出... 详细信息

发展电动汽车是实现“双碳”战略的重要手段,开展电动汽车充电特性及充电负荷有序调控研究具有重要意义。因此提出了计及用户行为决策的电动汽车充电负荷时空分布建模方法。首先,通过引入后悔理论模拟用户的充电行为决策。其次,结合出行链理论和后悔理论,对电动私家车出行行为的随机性和灵活性及充电负荷的时空不确定性进行出行特性建模。最后,结合美国交通部2009NHTS居民出行调研统计数据,运用蒙特卡洛方法建立电动汽车充电负荷时空分布模型。仿真结果验证了所提模型的合理性和有效性。

关键词：电动汽车用户行为决策充电负荷特性时空建模后悔理论出行链

牛蒡子苷元调控Notch/Hes-1信号通路对口腔鳞状细胞癌HSC-3细胞增殖、凋亡和侵袭的影响

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中国肿瘤生物治疗杂志 2024年第4期31卷 351-358页

作者：任丽洁刘孟媛史冠忠唐亮沧州医学高等专科学校口腔系河北沧州061000

目的:探究牛蒡子苷元(ARC)通过调控Notch/Hes-1信号通路对口腔鳞状细胞癌(OSCC)HSC-3细胞增殖、凋亡和侵袭的影响及其机制。方法:使用不同质量浓度的ARC处理人HSC-3细胞,CCK-8法检测ARC对细胞增殖活力的影响,以选择适宜的药物浓度。将HS... 详细信息

目的:探究牛蒡子苷元(ARC)通过调控Notch/Hes-1信号通路对口腔鳞状细胞癌(OSCC)HSC-3细胞增殖、凋亡和侵袭的影响及其机制。方法:使用不同质量浓度的ARC处理人HSC-3细胞,CCK-8法检测ARC对细胞增殖活力的影响,以选择适宜的药物浓度。将HSC-3细胞分为对照组、ARC-L组(10 mg/L ARC)、ARC-M组(20 mg/L ARC)、ARC-H组(40 mg/L ARC)和ARC-H+Jagged1/FC组(40 mg/L ARC+1.2μg/mL Jagged1/FC)。采用EdU法检测细胞增殖能力,划痕愈合实验、Transwell实验和流式细胞术分别检测细胞的迁移、侵袭能力及细胞周期和细胞凋亡率,WB法检测增殖(c-Myc、cyclin D1)、凋亡(BAX、Bcl-2、survivin)、EMT(E-cadherin、vimentin、Snail)及Notch/Hes-1通路(Notch 1、Hes-1、NICD)相关蛋白的表达水平。结果:与0 mg/L相比,10~80 mg/L的ARC均能显著降低HSC-3细胞增殖活力(均P<0.05)。与对照组相比,ARC-L组、ARC-M组和ARC-H组HSC-3细胞EdU阳性率、划痕愈合率、侵袭细胞数、S期和G2/M期细胞占比及c-Myc、cyclin D1、Bcl-2、survivin、vimentin、Snail、Notch 1、Hes-1和NICD蛋白表达均显著降低(均P<0.05),细胞凋亡率、G0/G1期细胞占比及BAX、E-cadherin的蛋白表达均显著升高(均P<0.05),且呈浓度梯度依赖性。同时使用Notch激动剂Jagged1/FC,则可部分逆转ARC对HSC-3细胞增殖、迁移、侵袭、凋亡及相关蛋白表达的作用(均P<0.05)。结论:ARC可能通过抑制Notch/Hes-1信号通路抑制OSCC细胞HSC-3增殖和侵袭并促进细胞凋亡。

关键词：牛蒡子苷元口腔鳞状细胞癌 HSC-3细胞增殖凋亡侵袭 Notch/Hes-1信号通路

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国内外新生儿听力筛查指南的质量评估及分析

听力学及言语疾病杂志 2023年第1期31卷 56-62页

作者：唐梦园黄丽辉文铖赵雪雷于一丁李悦首都医科大学附属北京同仁医院耳鼻咽喉头颈外科北京市耳鼻咽喉科研究所耳鼻咽喉头颈科学教育部重点实验室北京100730

目的系统梳理国内外新生儿听力筛查指南,对比各国指南相关信息异同,对指南进行质量评估,旨在为新生儿听力筛查(universal newborn hearing screening,UNHS)质量评估提供参考依据。方法从中英文数据库及政府网站检索UNHS相关指南,按照纳... 详细信息

目的系统梳理国内外新生儿听力筛查指南,对比各国指南相关信息异同,对指南进行质量评估,旨在为新生儿听力筛查(universal newborn hearing screening,UNHS)质量评估提供参考依据。方法从中英文数据库及政府网站检索UNHS相关指南,按照纳入及排除标准筛选,提取纳入指南基本信息和UNHS相关信息,由四位研究人员采用第二版临床指南研究与评估系统,对规定的6个领域23个条目及2个总体条目进行评分,计算各领域得分率、总体质量平均分、是否推荐使用指南人数及组内相关系数(ICC)。结果纳入指南14篇。UNHS相关信息中,所有指南推荐正常新生儿筛查流程为“初筛-复筛-诊断-干预”,71.43%(10/14)的指南推荐重症监护室筛查流程为“初筛-诊断-干预”;57.14%(8/14)及50%(7/14)的指南分别推荐正常新生儿初筛及复筛采用耳声发射或自动听性脑干反应,78.57%(11/14)的指南推荐重症监护室新生儿初筛采用自动听性脑干反应;21.43%(3/14)的指南推荐出院前初筛,50.00%(7/14)的指南推荐1个月内复筛,64.29%(9/14)的指南推荐3个月内诊断,71.43%(10/14)的指南推荐6个月内干预;35.71%(5/14)的指南推荐初筛覆盖率≥95%,14.29%(2/14)的指南推荐≥97%;35.71%(5/14)的指南推荐转诊率≤4%,7.14%(1/14)的指南推荐≤5%。质量评估中,领域4(清晰性)得分最高,领域6(独立性)得分最低,总体评分中美国和英国指南最高,均为6.25分;14篇指南的组内相关系数均在0.75及以上。结论14篇UNHS指南总体质量平均分较高,提示整体质量较好。大部分指南的筛查流程、筛查技术、初筛时间、复筛时间、诊断时间及干预时间一致。美国和英国指南评分较高,推荐可作为新生儿听力筛查质量评估参考依据。

关键词：新生儿听力筛查指南质量评估

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膜内麻雀优化ELM的软件缺陷预测算法

北京航空航天大学学报 2024年第2期50卷 643-654页

作者：唐宇代琪杨梦园陈丽芳华北理工大学理学院唐山063210 中国石油大学信息科学与工程学院北京102249 河北省数据科学与应用重点实验室唐山063210

原始麻雀搜索算法存在寻优精度低、迭代后期容易陷入局部极值的问题,结合高效寻优性能的改进麻雀搜索算法和具有并行计算能力的膜计算,提出一种膜内麻雀优化算法(IMSSA)。在10个CEC2017测试函数上的实验结果表明,IMSSA具有更高的寻优精... 详细信息

原始麻雀搜索算法存在寻优精度低、迭代后期容易陷入局部极值的问题,结合高效寻优性能的改进麻雀搜索算法和具有并行计算能力的膜计算,提出一种膜内麻雀优化算法(IMSSA)。在10个CEC2017测试函数上的实验结果表明,IMSSA具有更高的寻优精度。为进一步验证IMSSA的性能,使用IMSSA优化极限学习机(ELM)参数,提出一种膜内麻雀优化ELM(IMSSA-ELM)算法,并将其应用于软件缺陷预测领域。实验结果表明:在15个公开的软件缺陷数据集中,IMSSA-ELM算法预测性能在G-mean、MCC这2个评价指标下明显优于其他4种先进的对比算法,表明IMSSA-ELM算法具有更好的预测精度和稳定性,其实验结果在Friedman ranking和Holm’s post-hoc test非参数检验中具有明显的统计显著性。

关键词：改进麻雀搜索算法膜计算极限学习机优化算法软件缺陷预测

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