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The Crop Journal 2021年第2期9卷 456-464页

作者： Shuo Zhou Cuimian Zhang He Zhao mengyu lyu Fushuang Dong Yongwei Liu Fan Yang Haibo Wang Jianfang Chai Hebei Province Plant Genetic Engineering Center Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051HebeiChina

Wheat-rye T1BL·1RS translocation lines are widely used,especially in China,but their processing quality is generally *** interfering expression vector targeting theω-secalin genes was constructed with the 1Bx7 seed-specific ***-mediated genetic transformation of the wheat cultivar KN199 carrying the T1BL·1RS translocation generated 10 transgenic *** representative transgenic lines,8-2 and 13-7,were selected for *** with the control,the two transformants showed an up to 4.5-fold decrease in totalω-secalins and various levels of decrease inω-gliadins,γ-gliadins,and low-molecular-weight glutenins.A decrease in high molecular weight(HMW)glutenin 1Bx7 was detected only in 8-2,owing possibly to promoter *** levels ofα-gliadins were observed in both transformants,but increased levels of HMW glutenins were observed only in *** 13-7 showed increases in gluten index,Zeleny sedimentation value,stabilization time,and maximum *** bread volume was 849.6 mL,an 11.9%increase over that of the *** 8-2 showed decreases in these parameters,but its total cake-making quality score was 88,an 17.3%increase over that of the *** study demonstrates that the same RNAi construct may produce different effects on wheat processing quality and highlights the influence of the vector promoter in RNA interference.

关键词： T1BL·1RS Quality improvement RNA interference Secalin Triticum aestivum

Effect of Low Temperature Treatment on Agrobacterium-mediated Transformation of Maize Seed Buds

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Agricultural Biotechnology 2018年第3期7卷 75-76页

作者： mengyu lyu Fushuang DONG Junmin ZHANG He ZHAO Jianfang CHAI Shuo ZHOU Fan YANG Yongwei LIU Haibo WANG Plant Genetic Engineering Center of Hebei Province Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry Sciences

[Objectives]This study was conducted to improve the genetic transformation rate of maize. [Methods]The seeds of maize ＂ Zheng 58＂ as the experimental material were germinated and treated under freezing temperature of-18 ℃ for 30 and 35 min. The EGFP gene was transformed into the growth points of these seed buds by Agrobacterium tumefaciens EHA105. The transformation effect was determined by fluorescence protein detection on transformed buds. [Results]After a certain period of treatment at-18 ℃ following germination and transformation of maize bud growth points with A. tumefaciens,although the survival rate of maize buds was reduced,the percentage of transformed cells significantly increased. [Conclusions]Low temperature treatment can improve the transformation efficiency of A. tumefaciens to the growth point of maize bud.

关键词： Maize buds Low temperature treatment Agrobacterium Transgenic

Study on Differentiation of Growing Points in Corn Sprouts into Multiple Stems

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Agricultural Biotechnology 2020年第1期9卷 43-44页

作者： mengyu lyu Fushuang DONG Jianfang CHAI Shuo ZHOU He ZHAO Yongwei LIU Fan YANG Xiaoyu LI Junmin ZHANG Haibo WANG Institute of Genetics and Physiology Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei ProvinceShijiazhuang 050051China

[Objectives] This study was conducted to improve the efficiency of genetic transformation using growing points of corn sprouts. [Methods] A mixed liquid of plant hormones 6-BA and KT at a suitable concentration and ratio was added dropwise to the growing points of corn sprouts, followed by culture under appropriate conditions. [Results] Corn sprouts could be induced to form multiple plants, thereby improving the transformation efficiency of growing points in corn sprouts. [Conclusions] This study provides a breeding method using growing points of corn sprouts as transformation receptors for direct plantlet formation without tissue culture by the Agrobacterium transgenic technology.

关键词： Corn Sprout growing point Differentiation Plant

Effects of Acetylacetone Solution Soaking on Agrobacterium - transformed Maize Seed Buds

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Agricultural Biotechnology 2021年第3期10卷 11-14页

作者： mengyu lyu Fushuang DONG Shuo ZHOU Jianfang CHAI He ZHAO Yongwei LIU Fan YANG Bo JIAO Xiaoyu LI Junmin ZHANG Haibo WANG Plant Genetic Engineering Center of Hebei Province Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry Sciences(Agricultural Product Quality and Safety Research Center of Hebei Academy of Agricultural and Forestry Sciences)Shijiazhuang 050051China

[Objectives]The effect of acetosyringone seed soaking on the transformation of maize seed buds was analyzed,so as to improve the genetic transformation efficiency of maize and to provide technical support for transgenic breeding of maize.[Methods]The seeds of the"Zheng 58"maize inbred line were used as experimental *** the seeds were germinated,AS was added to the water at concentrations of 70,140,210,and 280μmol/L,respectively,and the seeds germinated without the addition of AS served as the *** Agrobacterium-mediated method was used to transform bud growth points of maize seeds,and green fluorescent protein detection was performed on the young shoots transformed with EGFP(enhanced green fluorescent protein)*** effect of soaking seeds with acetosyringone solution on the transformation of maize bud growth points by Agrobacterium was studied according to the detection results.[Results]Soaking seeds in acetosyringone solutions for germination had the effect of inhibiting the germination of maize seeds and inhibiting sprout elongation,and the higher the concentration of acetosyringone,the stronger the *** the concentration of acetosyringone solution was 280μmol/L,the germination rate of seeds was only 36.2%of the CK,while soaking seeds with 70-140μmol/L acetosyringone solution for germination could not only ensure a higher germination rate of maize seeds,but also significantly increased the transformation efficiency of maize bud growth *** the seeds were soaked with 70μmol/L acetosyringone solution for germination,the positive rate of transformed maize buds was the highest,reaching 32.1%.[Conclusions]When maize bud growth points were used as the receptor of Agrobacterium transformation,soaking seeds with 70-140μmol/L acetosyringone for germination basically did not affect the germination of seeds,and was beneficial to the activation of Agrobacterium,thereby promoting the transformation.

关键词： Maize Acetosyringone Seed soaking Transgenic efficiency

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时间分辨荧光免疫分析在环境中的应用进展

环境科学与技术 2019年第8期42卷 95-102页

作者：吕梦雨牛晓君彭志芳胡蹦荣天悦华南理工大学环境与能源学院工业聚集区污染控制与生态修复教育部重点实验室

时间分辨荧光免疫分析技术(TRFIA)作为一种新型的超微量分析技术,不断应用于环境痕量分析领域,在检测环境中的有机污染物、生物毒素等方面发挥了重大作用。该文论述了近年来开发的TRFIA新系统,阐述了TRFIA在检测环境中的药物残留、生物... 详细信息

时间分辨荧光免疫分析技术(TRFIA)作为一种新型的超微量分析技术,不断应用于环境痕量分析领域,在检测环境中的有机污染物、生物毒素等方面发挥了重大作用。该文论述了近年来开发的TRFIA新系统,阐述了TRFIA在检测环境中的药物残留、生物毒素及化工试剂等方面的研究进展,并在此基础上对其应用前景进行了分析,展望了其在检测新兴有机污染物方面的巨大潜力。

关键词：时间分辨荧光分析环境污染物痕量分析

16.48% Efficient solution-processed CIGS solar cells with crystal growth and defects engineering enabled by Ag doping strategy

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Journal of Energy Chemistry 2025年第1期100卷 59-65页

作者： mengyu Xu Shaocong Yan Ting Liang Jia Jia Shengjie Yuan Dongxing Kou Zhengji Zhou Wenhui Zhou Yafang Qi Yuena Meng Litao Han Sixin Wu Key Laboratory for Special Functional Materials of MOE National & Local Joint Engineering Research Centre for High-efficiency Display and Lighting Technology School of Nanoscience and Materials Engineering Henan University

Solution-processed Cu（In,Ga）Se2（CIGS） solar cells suffer from serious carrier recombination and power conversion efficiency（PCE） loss because of the poor film properties and easy formation of ***, we propose Ag&Se co-selenization strategy to enhance the crystallization and passivate harmful defects of the CIGS films. The formation of Ag-Se phase during the selenization process enables the formation of large grains and suppresses the deep level defects. It is found that Ag doping can enlarge the depletion region width, lower the Urbach energy and prolong the carrier lifetime. As a result, a champion solution-processed CIGS solar cell presents a high efficiency of 16.48% with the highly improved opencircuit voltage（VOC） of 662 m V and fill factor（FF） of 75.8%. This work provides an efficient strategy to prepare high quality solution-processed CIGS films for high-performance CIGS solar cells.

关键词： Cell engineering

解毒复正汤通过干扰miR-223-3p并靶向调控TGFBR3影响肺癌侵袭转移的实验研究

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世界科学技术-中医药现代化 2023年第4期25卷 1358-1366页

作者：姚菲石玮吕梦玉吴姗燕王家晓李春姗广西中医药大学第一附属医院南宁530200

目的探讨解毒复正汤(JieduFuzhengdecoction,JDFZ)通过影响微小核糖核酸-223-3p(Mircro ribonucleic acid-223-3p,miR-223-3p)靶向调控转化生长因子β受体3(Transforming growth factorβReceptor3,TGFBR3)的表达及其对肺癌细胞迁移的影响。方法利用RT-qPCR技术检测肺腺癌患者癌组织及癌旁组织、肺腺癌患者及健康人外周血清中miR-223-3p表达水平的差异;并检测不同种类的NSCLC细胞系肺癌细胞(95D、LTEP-α-2、A549及NCI-H460)以及人肺上皮细胞BEAS-2B中miR-223-3p的本底表达量,从组织、血浆及细胞3个维度验证miR-223-3p的差异性。利用生物信息学网站重叠分析,初步预测到miR-223-3p与TGFBR33’UTR具有潜在的结合位点序列。接着运用双荧光素酶报告基因实验验证miR-223-3p与TGFBR3的靶向关系。体外培养肺癌A549细胞,采用脂质体法分别将miR-223-3p-过表达序列(miR-223-3p-mimics)及携带TGFBR3全基因的重组载体(Over-expression-TGFBR3,OE-TGFBR3)分别转染至肺癌细胞,以转入无关序列的模拟剂(Negative control-mimics,NC)作为对照组,收集各组稳转细胞,Transwell小室检测转染处理后及JDFZ作用后的细胞迁移能力;收集各组细胞蛋白,蛋白免疫印迹法(Westernblot)检测细胞中EMT相关蛋白的表达。结果肺癌组织中miR-223-3p的表达量显著低于癌旁组织;肺癌患者血浆miR-223-3p表达水平明显低于健康正常人。不同种类的NSCLC细胞系肺癌细胞(95D、LTEP-α-2、A549及NCI-H460)中的miR-223-3p表达量亦低于正常人肺上皮细胞BEAS-2B。利用生物信息学网站及荧光素酶报告基因实验证实了miR-223-3p与TGFBR3的靶向调控关系。与miR-223-3p NC组对比,miR-223-3p mimics组和miR-223-3p mimics+OE-TGFBR3组的细胞迁移能力均下降(P<0.05),上调TGFBR3和加入解毒复正汤均能有效增强miR-223-3p对A549细胞迁移的负向调节作用;Westenblot结果发现,与miR-223-3p NC组对比,miR-223-3p mimics组和miR-223-3p mimics+OE-TGFBR3组E-Cadherin的表达水平上调,NCadherin及Vimentin的表达水平下升(P<0.05),两组均在不同程度上抑制EMT的发生;上调TGFBR3和加入解毒复正汤作用能有效增加miR-223-3p对细胞迁移相关蛋白的负向调节作用(P<0.05)。结论解毒复正汤对肺癌A549细胞的侵袭迁移有抑制作用,其机制可能是通过调节miR-223-3p并靶向调控TGFBR3及其下游EMT相关指标的表达来实现的。

关键词：微小核糖核酸-223-3p(miR-223-3p) 人转化生长因子β受体3(TGFBR3) 解毒复正汤侵袭转移肺癌

Localizing base stations with measured data:a concatenated image-based deep learning approach

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Science China(Information Sciences) 2025年

作者： Yang CHEN Ziyuan LIU Feng JIANG Hong SHEN Wei XU mengyu LI Ritao CHENG National Mobile Communications Research Laboratory Southeast University China Mobile Group Design Institute Co. Ltd.

Localization has become an indispensable function of modern cellular communication systems[1]. In a cloud radio access network （C-RAN）, the remote radio head （RRH） is the actual signal transmitter, whose location ...

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艾滋病病毒感染者快感缺失的相关因素

中国心理卫生杂志 2024年第6期38卷 542-546页

作者：李云红鲁丽莉陈雪宇吕梦玉赵霞杨婷蔡琳成都市公共卫生临床医疗中心成都610066

目的:探讨人类免疫缺陷病毒(HIV)/艾滋病(AIDS)患者的快感缺失的相关因素。方法:选取某传染病医院HIV/AIDS患者237例,采用自编一般资料调查表、时间性愉快体验量表(TEPS)、自我接纳量表(SAQ)、歧视知觉量表(SIS)、领悟社会支持量表(PSSS... 详细信息

目的:探讨人类免疫缺陷病毒(HIV)/艾滋病(AIDS)患者的快感缺失的相关因素。方法:选取某传染病医院HIV/AIDS患者237例,采用自编一般资料调查表、时间性愉快体验量表(TEPS)、自我接纳量表(SAQ)、歧视知觉量表(SIS)、领悟社会支持量表(PSSS)进行调查。结果:患者TEPS得分为(73.4±16.1)。逐步线性回归分析显示PSSS总分、受教育程度、个人月收入与TEPS总分正向关联(β=0.41、5.17、4.63),年龄与TEPS总分负向关联(β=-0.30)。结论:HIV/AIDS患者的快感缺失情况需引起重视,患者的快感缺失与个人月收入、受教育程度、年龄、领悟社会支持相关。

关键词：人类免疫缺陷病毒(HIV) 艾滋病(AIDS) 快感缺失

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放射性肺炎的诊疗研究进展

中国现代医生 2021年第6期59卷 187-192页

作者：吕梦宇裘关关裘淼熊中奎浙江省绍兴第二医院药剂科浙江绍兴312000 浙江省绍兴第二医院肿瘤放疗科浙江绍兴312000

放射性肺炎是胸部肿瘤放疗常见的严重并发症,虽然放疗技术有了革命性的提高,但放射性肺炎的发生率仍不低,严重者将危及生命。放射性肺炎一旦发展至放射性肺纤维化将不可逆转,故预防放射性肺炎的发生尤为重要。放射性肺炎的临床表现主要... 详细信息

放射性肺炎是胸部肿瘤放疗常见的严重并发症,虽然放疗技术有了革命性的提高,但放射性肺炎的发生率仍不低,严重者将危及生命。放射性肺炎一旦发展至放射性肺纤维化将不可逆转,故预防放射性肺炎的发生尤为重要。放射性肺炎的临床表现主要为呼吸困难、咳嗽、发热及肺衰竭,常用糖皮质激素及抗生素治疗。随着放疗技术的不断发展,放射性肺炎日渐受到重视。本文着重介绍放射性肺炎的诊断、防治手段及相关最新研究进展。

关键词：放射性肺炎放疗抗肿瘤防治综述