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New xylose transporters support the simultaneous consumption of glucose and xylose in Escherichia coli

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mLife 2022年第2期1卷 156-170页

作者： Xinna Zhu Feiyu Fan Huanna Qiu mengyao shao Di Li Yong Yu Changhao Bi Xueli Zhang Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjinChina Key Laboratory of Systems Microbial Biotechnology Chinese Academy of SciencesTianjinChina College of Biotechnology Tianjin University of Sciences and TechnologyTianjinChina University of Chinese Academy of Sciences BeijingChina

Glucose and xylose are two major components of *** consumption of glucose and xylose is critical for engineered microorganisms to produce fuels and chemicals from lignocellulosic *** many production limitations have been resolved,glucose‐induced inhibition of xylose transport remains a *** this study,a cell growthbased screening strategy was designed to identify xylose transporters uninhibited by *** glucose pathway was genetically blocked in Escherichia coli so that glucose functions only as an inhibitor and cells need xylose as the carbon source for *** adaptive evolution,omics analysis and reverse metabolic engineering,a new phosphoenolpyruvate:carbohydrate phosphotransferase system(PTS)galactitol transporter(GalABC,encoded by EcolC_1640,EcolC_1641,and EcolC_1642 genes)that is not inhibited by glucose was *** of adenylate cyclase led to increased expression of the EcolC_1642 gene,and a point mutation in gene EcolC_1642(N13S)further enhanced xylose *** the second round of gene mining,AraE and a new ABC transporter(AraFGH)of xylose were identified.A point mutation in the transcription regulator araC(L156I)caused increased expression of araE and araFGH genes without arabinose induction,and a point mutation in araE(D223Y)further enhanced xylose *** newly identified xylose transporters can support the simultaneous consumption of glucose and xylose and have potential use in producing chemicals from lignocellulose.

关键词： ABC transporter glucose PTS xylose xylose transporter

Embodied exergy-based assessment of energy and resource consumption of buildings

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Frontiers of Earth Science 2014年第1期8卷 150-162页

作者： Jing MENG Zhi LI Jiashuo LI Ling shao mengyao HAN Shan GUO College of Urban and Environmental Sciences Peking University Beijing 100871 China State Key Laboratory for Turbulence and Complex Systems College of Engineering Peking University Beijing 100871 China

As an effective approach to achieve a more unified and scientific assessment, embodied exergy-based analysis is devised to assess the energy and resource consumption of buildings. A systematic accounting of the landmark buildings in E-town, Beijing is performed, on the basis of raw project data in the Bill of Quantities （BOQ） and the most recent embodied exergy intensities for the Chinese economy in 2007 with 135 industrial sectors. The embodied exergy of the engineering structure of the case buildings is quantified as 4.95E ＋ 14 J, corresponding to an intensity of 8.25E ＋ 09 J/m2 floor area. Total exergy of 51.9% and 28.8% are embodied in the steel and concrete inputs, respectively, due to the fact that the case buildings are structured of reinforced-concrete. The fossil fuel source （coal, crude oil, and natural gas） is predominant among four categories of natural resources （fossil fuel, biological, mineral, and environmental）, accounting for 89.9% of the embodied exergy, with coal as the dominant energy resource （75.5%）. The material accounts for 89.5% of the embodied exergy, in contrast to 9.0%, 1.4%, and 0.1% for manpower, energy, and equipment respectively. This result indicates that great attention should be given to the use of various materials vs. their value of their contribution

关键词： energy resources exergy analysis greenbuilding ecological assessment

Multimodal Nature of the Single-cell Primate Brain Atlas:Morphology,Transcriptome,Electrophysiology,and Connectivity

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Neuroscience Bulletin 2024年第4期40卷 517-532页

作者： Yuhui Shen Mingting shao Zhao-Zhe Hao mengyao Huang Nana Xu Sheng Liu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangzhou510060China Guangdong Province Key Laboratory of Brain Function and Disease Guangzhou510080China

Primates exhibit complex brain structures that augment cognitive *** neocortex fulfills high-cognitive functions through billions of connected *** neurons have distinct transcriptomic,morphological,and electrophysiological properties,and their connectivity principles *** features endow the primate brain atlas with a multimodal *** recent integration of next-generation sequencing with modified patch-clamp techniques is revolutionizing the way to census the primate neocortex,enabling a multimodal neuronal atlas to be established in great detail:(1)single-cell/single-nucleus RNA-seq technology establishes high-throughput transcriptomic references,covering all major transcriptomic cell types;(2)patch-seq links the morphological and electrophysiological features to the transcriptomic reference;(3)multicell patch-clamp delineates the principles of local ***,we review the applications of these technologies in the primate neocortex and discuss the current advances and tentative gaps for a comprehensive understanding of the primate neocortex.

关键词： Neuron Brain atlas Patch-seq Connectivity Primate

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两因素非参数方差分析模型的构建及其应用

黑龙江科学 2024年第24期15卷 34-37页

作者：李鑫邵梦瑶李玲玲西安工程大学理学院西安710600 陕西师范大学数学与统计学院西安710119

方差分析是研究影响因素是否显著性的一个常用方法,要求数据满足正态分布且具有方差齐性,但在很多实际问题研究中往往不能满足这些条件,因此非参数统计方法是一个很好的选择。目前,常见的有Kruskal-Wallis单因素方差分析在实际问题中往... 详细信息

方差分析是研究影响因素是否显著性的一个常用方法,要求数据满足正态分布且具有方差齐性,但在很多实际问题研究中往往不能满足这些条件,因此非参数统计方法是一个很好的选择。目前,常见的有Kruskal-Wallis单因素方差分析在实际问题中往往需要考虑多因素及其交互效应的影响,这时单因素非参数方差分析就不再适用,故探讨两因素非参数方差分析的理论及其在实践中的应用。基于Kruskal-Wallis单因素方差分析推导出两因素非参数方差分析的理论模型,选取实体癌的发病率数据,应用该模型进行分析,评估离子辐射剂量和辐射暴露时长对实体癌发病率的影响,结合logistic回归模型对两因素非参数方差分析模型进行验证。结果显示,两因素非参数方差分析和Logistic回归分析的结果是一致的,表明离子辐射剂量和辐射暴露时长均对实体癌风险有显著影响,且离子辐射剂量和辐射暴露时长的交互效应并不显著。本研究为两因素非参数方差分析在癌症风险评估中的应用提供了理论基础,通过实际案例展示了其在实践中的实用性。

关键词：方差分析非参数检验 Kruskal-Wallis检验 logistic回归模型

KIF5B-mediated internalization of FMDV promotes virus infection

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Virologica Sinica 2024年第3期39卷 378-389页

作者： Wei Zhang Fan Yang Yang Yang Weijun Cao Wenhua shao Jiali Wang mengyao Huang Zhitong Chen Xiaoyi Zhao Weiwei Li Zixiang Zhu Haixue Zheng State Key Laboratory for Animal Disease Control and Prevention College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730000China Gansu Province Research Center for Basic Disciplines of Pathogen Biology Lanzhou 730046China

Foot-and-mouth disease(FMD)is a highly contagious and economically important disease,which is caused by the FMD virus(FMDV).Although the cell receptor for FMDV has been identified,the specific mechanism of FMDV internalization after infection remains *** this study,we found that kinesin family member 5B(KIF5B)plays a vital role during FMDV ***,we confirmed the interaction between KIF5B and FMDV structural protein VP1 by co-immunoprecipitation(Co-IP)and co-localization in FMDV-infected *** particular,the stalk[amino acids(aa)413–678]domain of KIF5B was indispensable for KIF5B-VP1 ***,overexpression of KIF5B dramatically enhanced FMDV replication;consistently,knockdown or knockout of KIF5B suppressed FMDV ***,we also demonstrated that KIF5B promotes the internalization of FMDV via regulating clathrin ***5B also promotes the transmission of viral particles to early and late endosomes during the early stages of *** conclusion,our results demonstrate that KIF5B promotes the internalization of FMDV via regulating clathrin uncoating and intracellular *** study may provide a new therapeutic target for developing FMDV antiviral drugs.

关键词： FMDV VP1 protein KIF5B Endosome Clathrin

Superhydrophobic Surface-Assisted Preparation of Microspheres and Supraparticles and Their Applications

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Nano-Micro Letters 2024年第4期16卷 110-138页

作者： mengyao Pan Huijuan shao Yue Fan Jinlong Yang Jiaxin Liu Zhongqian Deng Zhenda Liu Zhidi Chen Jun Zhang Kangfeng Yi Yucai Su Dehui Wang Xu Deng Fei Deng Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 611731People’s Republic of China School of Materials Science and Engineering Sun Yat-Sen UniversityGuangzhou 510275People’s Republic of China Shenzhen Institute for Advanced Study University of Electronic Science and Technology of ChinaShenzhen 518110People’s Republic of China Pharmaceutical Glass Co.Ltd Zibo 256100People’s Republic of China Department of Nephropathy School of MedicineSichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengduPeople’s Republic of China Department of Nephrology Sichuan Provincial People’s Hospital Jinniu HospitalChengdu Jinniu District People’s HospitalChengduPeople’s Republic of China

Superhydrophobic surface(SHS) has been well developed, as SHS renders the property of minimizing the water/solid contact interface. Water droplets deposited onto SHS with contact angles exceeding 150°, allow them to retain spherical shapes, and the low adhesion of SHS facilitates easy droplet collection when tilting the substrate. These characteristics make SHS suitable for a wide range of applications. One particularly promising application is the fabrication of microsphere and supraparticle materials. SHS offers a distinct advantage as a universal platform capable of providing customized services for a variety of microspheres and supraparticles. In this review, an overview of the strategies for fabricating microspheres and supraparticles with the aid of SHS, including cross-linking process, polymer melting,and droplet template evaporation methods, is first presented. Then, the applications of microspheres and supraparticles formed onto SHS are discussed in detail, for example, fabricating photonic devices with controllable structures and tunable structural colors, acting as catalysts with emerging or synergetic properties, being integrated into the biomedical field to construct the devices with different medicinal purposes, being utilized for inducing protein crystallization and detecting trace amounts of analytes. Finally,the perspective on future developments involved with this research field is given, along with some obstacles and opportunities.

关键词： Superhydrophobic surface Microspheres and supraparticles Photonic devices Catalysts Biomedical and trace detections

Survey and Analysis on Undergraduate Tea Consumption and Attitude towards Tea Consumption

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Asian Agricultural Research 2013年第5期5卷 118-120,124页

作者： Jiahua LI Xiaohui LU mengyao JIANG Wanfang shao Jianyun ZHOU College of Longrun Pu-erh Tea Yunnan Agricultural University School of Aerospace Tsinghua University

Based on the sample survey of undergraduates from Tsinghua University, Northwest Sci-Tech University of Agriculture and Forestry, Yunnan University and Yunnan Agricultural University, this paper analyzes their tea consumption and attitude towards tea consumption. Results show that more than 50% undergraduates like drinking tea, especially green tea. They would like to accept lower price tea, prefer quality to brand, and have higher degree of approval of new methods of tea drinking and tea products. However, most undergraduates lack relevant knowledge about identifying tea quality. Finally, it presents recommendations for expanding tea consumption market among undergraduates.

关键词： Tea Undergraduates Consumption Consumption attitud

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分子梭协助离子跨膜运输的机理研究

高等学校化学学报 2021年第10期42卷 3116-3124页

作者：妙孟姚郭一畅邵学广蔡文生南开大学化学学院分析科学研究中心天津市生物传感与分子识别重点实验室药物化学生物学国家重点实验室天津300071

采用增强采样分子动力学模拟研究了一轮烷型分子梭协助K^(+)跨膜转运的机制,该轮烷由两亲性的轴和套在轴上的大冠醚环及连接在大冠醚环上的小冠醚环构成,轴上有3个带正电的结合位点.通过计算穿梭过程的自由能变化,探索了溶剂(氯仿、乙... 详细信息

采用增强采样分子动力学模拟研究了一轮烷型分子梭协助K^(+)跨膜转运的机制,该轮烷由两亲性的轴和套在轴上的大冠醚环及连接在大冠醚环上的小冠醚环构成,轴上有3个带正电的结合位点.通过计算穿梭过程的自由能变化,探索了溶剂(氯仿、乙腈、水、氯仿-乙腈)以及中间结合位点对该轮烷穿梭运动的影响,并分析了中间位点在其携带K^(+)穿越细胞膜(采用水-氯仿-水模拟)过程中的重要作用.结果表明,改变溶剂不会改变轮烷(不携带K^(+))的运动模式,但随着溶剂极性的增加穿梭所需克服的自由能能垒显著降低;在氯仿-乙腈混合溶剂中,中间结合位点的质子化状态,不影响轮烷(不携带K^(+))的穿梭能垒;然而在模拟细胞膜的环境中,该结合位点的质子化与去质子化相比大幅降低了穿梭的能垒,促进了K^(+)的跨膜转运,表明中间位点的质子化对于离子转运至关重要,进一步分析表明轮烷中大环穿梭和小环摆动的协同作用,也是加速离子跨膜转运的另一关键因素.

关键词：人工离子转运体轮烷自由能计算质子化协同运动

基于深度学习的SAR辅助下光学遥感图像去云方法

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光学学报 2021年第12期41卷 235-243页

作者：王梦瑶孟祥超邵枫符冉迪宁波大学信息科学与工程学院浙江宁波315211

针对现有基于深度学习的SAR辅助下光学图像去云方法对光学图像纹理、光谱信息考虑不足,导致去云后影像往往存在模糊、光谱损失等现象,根据杭州市余杭区哨兵一号(Sentinel-1)和哨兵二号(Sentinel-2)卫星影像,构建了SAR辅助下光学图像去... 详细信息

针对现有基于深度学习的SAR辅助下光学图像去云方法对光学图像纹理、光谱信息考虑不足,导致去云后影像往往存在模糊、光谱损失等现象,根据杭州市余杭区哨兵一号(Sentinel-1)和哨兵二号(Sentinel-2)卫星影像,构建了SAR辅助下光学图像去云数据库,并充分考虑光学遥感图像的细节、纹理以及色彩信息,建立了基于条件生成对抗网络(conditional generative adversarial network,cGAN)的SAR辅助下的光学遥感图像去云模型,实现了SAR辅助下光学图像薄云、雾、厚云等覆盖下地物信息的有效复原与重建。实验结果表明,所提方法可有效实现SAR辅助下的光学图像去云,相比于其他方法具有更优性能。

关键词：遥感云去除条件生成对抗网络(cGAN) 合成孔径雷达(SAR)图像光学图像影像融合