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Doxorubicin-loaded bacterial outer-membrane vesicles exert enhanced anti-tumor efficacy in non-small-cell lung cancer

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Acta Pharmaceutica Sinica B 2020年第8期10卷 1534-1548页

作者： Kudelaidi Kuerban Xiwen Gao Hui Zhang Jiayang Liu mengxue dong Lina Wu Ruihong Ye Meiqing Feng Li Ye Minghang Hospital&Department of Biological Medicines at School of Pharmacy Fudan UniversityShanghai 201100China Shanghai Engineering Research Center of ImmunoTherapeutics School of PharmacyFudan UniversityShanghai 201203China TOF-PET/CT/MR Center the 4th Affiliated HospitalHarbin Medical UniversityHarbin 150028China Department of Pharmaceutical Engineering School of PharmacyHubei University of Chinese MedicineWuhan 430070China

More efficient drug delivery system and formulation with less adverse effects are needed for the clinical application of broad-spectrum antineoplastic agent doxorubicin(DOX). Here we obtained outer-membrane vesicles(OMVs), a nano-sized proteoliposomes naturally released by Gram-negative bacteria, from attenuated Klebsiella pneumonia and prepared doxorubicin-loaded O0MVs(DOX-OMV). Confocal microscopy and in vivo distribution study observed that DOX encapsulated in OMVs was efficiently transported into NSCLC A549 cells. DOX-OMV resulted in intensive cytotoxic effects and cell apoptosis in vitro as evident from MTT assay, Western blotting and flow cytometry due to the rapid cellular uptake of DOX. In A549 tumor-bearing BALB/c nude mice, DOX-OMV presented a substantial tumor growth inhibition with favorable tolerability and pharmacokinetic profile, and TUNEL assay and H&E staining displayed extensive apoptotic cells and necrosis in tumor tissues. More importantly, OMVs’ appropriate immunogenicity enabled the recruitment of macrophages in tumor microenvironment which might synergize with their cargo DOX in vivo. Our results suggest that OMVs can not only function as biological nanocarriers for chemotherapeutic agents but also elicit suitable immune responses, thus having a great potential for the tumor chemoimmunotherapy.

关键词： nude loaded outer

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PMN-MDSCs modulated by CCL20 from cancer cells promoted breast cancer cell stemness through CXCL2-CXCR2 pathway

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1828-1841页

作者： Rui Zhang mengxue dong Juchuanli Tu Fengkai Li Qiaodan Deng Jiahui Xu Xueyan He Jiajun Ding Jie Xia Dandan Sheng Zhaoxia Chang Wei Ma Haonan dong Yi Zhang Lixing Zhang Lu Zhang Suling Liu Fudan University Shanghai Cancer Center&Institutes of Biomedical Sciences State Key Laboratory of Genetic EngineeringCancer InstitutesKey Laboratory of Breast Cancer in ShanghaiThe Shanghai Key Laboratory of Medical EpigeneticsShanghai Key Laboratory of Radiation OncologyThe International Co-laboratory of Medical Epigenetics and MetabolismMinistry of Science and TechnologyShanghai Medical CollegeFudan UniversityShanghai 200032China Breast Surgery Obstetrics and Gynecology Hospital of Fudan UniversityShanghaiChina Department of Breast and Thyroid Surgery Southwest Hospitalthe First Affiliated Hospital of the Army Military Medical UniversityChongqing 400038China Jiangsu Key Lab of Cancer Biomarkers Prevention and TreatmentCollaborative Innovation Center for Cancer MedicineNanjing Medical UniversityNanjing 211166China

Our previous studies have showed that C-C motif chemokine ligand 20(CCL20)advanced tumor progression and enhanced the chemoresistance of cancer cells by positively regulating breast cancer stem cell(BCSC)***,it is unclear whether CCL20 affects breast cancer progression by remodeling the tumor microenvironment(TME).Here,we observed that polymorphonuclear myeloid-derived suppressor cells(PMN-MDSCs)were remarkably enriched in TME of CCL20-overexpressing cancer cell orthotopic allograft ***,CCL20 activated the differentiation of granulocyte-monocyte progenitors(GMPs)via its receptor C-C motif chemokine receptor 6(CCR6)leading to the PMN-MDSC ***-MDSCs from CCL20-overexpressing cell orthotopic allograft tumors(CCL20-modulated PMN-MDSCs)secreted amounts of C-X-C motif chemokine ligand 2(CXCL2)and increased ALDH+BCSCs via activating CXCR2/NOTCH1/HEY1 signaling ***,C-X-C motif chemokine receptor 2(CXCR2)antagonist SB225002 enhanced the docetaxel(DTX)effects on tumor growth by decreasing BCSCs in CCL20high-expressing *** findings elucidated how CCL20 modulated the TME to promote cancer development,indicating a new therapeutic strategy by interfering with the interaction between PMN-MDSCs and BCSCs in breast cancer,especially in CCL20high-expressing breast cancer.

关键词： CCL20 CXCR2 breast

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Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1

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Protein & Cell 2024年第6期15卷 419-440页

作者： Wei Ma Lu Zhang Weilong Chen Zhaoxia Chang Juchuanli Tu Yuanyuan Qin Yuwen Yao mengxue dong Jiajun Ding Siqin Li Fengkai Li Qiaodan Deng Yifei Yang Tingting Feng Fanrong Zhang Xiying Shao Xueyan He Lixing Zhang Guohong Hu Quentin Liu Yi-Zhou Jiang Shu Zhu Zhi Xiao Dan Su Tong Liu Suling Liu Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences State Key Laboratory of Genetic EngineeringCancer InstitutesDepartment of OncologyKey Laboratory of Breast Cancer in ShanghaiThe Shanghai Key Laboratory of Medical EpigeneticsShanghai Key Laboratory of Radiation OncologyThe International Co-laboratory of Medical Epigenetics and MetabolismMinistry of Science and TechnologyShanghai Medical CollegeFudan UniversityShanghai 200032China Intelligent Pathology Institute and Department of Pathology The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230071China Department of Thyroid Breast and Vascular SurgeryXijing HospitalFourth Military Medical UniversityXi’an 710032China Institute of Immunology CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefei 230027China Department of Pathology The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)Institute of Basic Medicine and Cancer (IBMC)Chinese Academy of SciencesHangzhou 310022China Department of Breast Surgery The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)Institute of Basic Medicine and Cancer (IBMC)Chinese Academy of SciencesHangzhou 310022China Department of Breast Medical Oncology The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)Institute of Basic Medicine and Cancer (IBMC)Chinese Academy of SciencesHangzhou 310022China Shanghai Institute of Nutrition and Health University of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaGuangzhou 510060China Department of Breast Surgery Xiangya HospitalChangsha 410008China Department of Breast Surgery Tumor Hospital of Harbin Medic

Tumor-resident microbiota in breast cancer promotes cancer initiation and malignant ***,targeting microbiota to improve the effects of breast cancer therapy has not been investigated in ***,we evaluated the microbiota composition of breast tumors and found that enterotoxigenic Bacteroides fragilis(ETBF)was highly enriched in the tumors of patients who did not respond to taxane-based neoadjuvant ***,albeit at low biomass,secreted the toxic protein BFT-1 to promote breast cancer cell stemness and *** studies showed that BFT-1 directly bound to NOD1 and stabilized NOD1 ***1 was highly expressed on ALDH+breast cancer stem cells(BCSCs)and cooperated with GAK to phosphorylate NUMB and promote its lysosomal degradation,thereby activating the NOTCH1-HEY1 signaling pathway to increase ***1 inhibition and ETBF clearance increase the chemosensitivity of breast cancer by impairing BCSCs.

关键词： NOD1 breast chemotherapy

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高压大功率S^3MPR电源控制器设计与验证

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航天器工程 2020年第2期29卷 59-66页

作者：谢伟靳洋董宝磊董梦雪蓝建宇林志松何小斌上海空间电源研究所空间电源国家重点实验室上海200245

针对航天器平台一级能源变换高压大功率的应用需求,采用并联式DC/DC变换器的最大功率点跟踪(MPPT)拓扑结构,设计一种顺序开关分流最大功率调节(S^3MPR)电源控制器。为了解决传统S^3MPR技术的算法电路复杂、跟踪精度低等问题,兼顾航天器... 详细信息

针对航天器平台一级能源变换高压大功率的应用需求,采用并联式DC/DC变换器的最大功率点跟踪(MPPT)拓扑结构,设计一种顺序开关分流最大功率调节(S^3MPR)电源控制器。为了解决传统S^3MPR技术的算法电路复杂、跟踪精度低等问题,兼顾航天器应用环境的特殊性,通过采用模拟电路实现的方式设计出一种基于交错扰动方法的MPPT控制算法电路。该算法可以实现太阳电池阵电压电流输出较大范围内精确跟踪其最大功率输出。研制了5 kW功率(10路太阳电池阵)的原理样机对设计进行试验验证,结果表明:电源控制器性能良好,满足高压大功率航天器平台的应用需求。

关键词：电源控制器顺序开关分流最大功率调节器限频分流电路最大功率点跟踪跟踪精度

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地铁列车进站过程中活塞风对站台屏蔽门的影响——以杭州地铁为例

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河南科学 2021年第10期39卷 1575-1580页

作者：董梦雪赵帅帅杭州万向职业技术学院杭州310023

以活塞风为研究对象,采用SST k-ω模型进行非定常计算,研究了地铁列车进站过程中产生的活塞风对站台全封闭屏蔽门的影响.研究结果表明,在列车进站过程中,活塞风井对活塞风的疏导作用与列车位置相关,距离车头位置越近的活塞风井对活塞风... 详细信息

以活塞风为研究对象,采用SST k-ω模型进行非定常计算,研究了地铁列车进站过程中产生的活塞风对站台全封闭屏蔽门的影响.研究结果表明,在列车进站过程中,活塞风井对活塞风的疏导作用与列车位置相关,距离车头位置越近的活塞风井对活塞风的疏导作用越大;列车在进站过程中会引起屏蔽门表面压力发生变化,该压力在车头通过第一个活塞风井后达到峰值,站台屏蔽门破损风险较大,且越靠近该活塞风井的屏蔽门表面压力波动幅值越大;在列车进站过程中,活塞风井内存在着回流区,且该回流区不断发展演化,与列车对空气挤压产生的活塞风相互叠加,导致屏蔽门表面压力反复振荡.

关键词：活塞风地铁列车站台屏蔽门非定常流动

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Enhanced solid-electrolyte interface efficiency for practically viable hydrogen-air fuel cell systems

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Journal of Energy Chemistry 2025年第1期100卷 356-368页

作者： Venkitesan Sakthivel dong Jin Yoo Graduate School Department of Energy Storage/Conversion Engineering (BK21 FOUR) Hydrogen and Fuel Cell Research Center Jeonbuk National University Jeonju Department of Life Science Jeonbuk National University

Proton exchange membrane fuel cells （PEMFCs） provide an appealing sustainable energy system,with the solid-electrolyte membrane playing a crucial role in its overall ***,sulfonated poly（1,4-phenylene ether-ether sulfone）(SPEES),an aromatic hydrocarbon polymer,has garnered considerable attention as an alternative to Nafion ***,the long-term durability and stability of SPEES present a significant *** this context,we introduce a potential solution in the form of an additive,specifically a core–shell-based amine-functionalized iron titanate （A–Fe2TiO5）,which holds promise for improving the lifetime,proton conductivity,and power density of SPEES in *** modified SPEES/A–Fe2TiO5composite membranes exhibited notable characteristics,including high water uptake,enhanced thermomechanical stability,and oxidative ***,the SPEES membrane loaded with 1.2 wt%of A–Fe2TiO5demonstrates a maximum proton conductivity of 155 mS cm-1,a twofold increase compared to the SPEES membrane,at 80°C under 100%relative humidity （RH）.Furthermore,the 1.2 wt%of A–Fe2TiO5/SPEES composite membranes exhibited a maximum power density of 397.37 mW cm-2and a current density of 1148 mA cm-2at 60°C under 100%RH,with an opencircuit voltage decay of 0.05 m V/h during 103 h of continuous *** study offers significant insights into the development and understanding of innovative SPEES nanocomposite membranes for PEMFC applications.

关键词： Acid-base Hybrid membrane Proton conductivity Polymer electrolyte fuel cell Single-cell performance

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一种无通信的无线电能传输阻抗匹配方法

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电源学报 2022年第1期20卷 165-172页

作者：吴昕何小斌蓝建宇邹小雨谢伟董梦雪上海空间电源研究所空间电源技术国家重点实验室上海200245

磁耦合谐振式无线电能传输系统由于线圈两侧的电气隔离,通常使用无线通信来实时获取原副边的电压电流信息并对其进行控制。在一些特殊应用场合比如航天器,由于频率管制等原因无法使用通信电路。为了解决该问题同时使得系统的传输效率最... 详细信息

磁耦合谐振式无线电能传输系统由于线圈两侧的电气隔离,通常使用无线通信来实时获取原副边的电压电流信息并对其进行控制。在一些特殊应用场合比如航天器,由于频率管制等原因无法使用通信电路。为了解决该问题同时使得系统的传输效率最优,提出了一种无通信的阻抗匹配方法。该方法选用Buck电路作为阻抗匹配网络,在负载电压变化时采用线性拟合的方法对整流输出电压进行控制,进而实现对系统最大效率的跟踪。建立了系统的等效电路模型,在不同负载下对系统的效率曲线进行了分析,给出了系统的控制策略。仿真与实验表明,在10 cm的传输距离下系统的效率保持87%以上,验证了无通信阻抗匹配方法的可行性。

关键词：无线电能传输无通信最大效率跟踪阻抗匹配

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Effect of lamellarization on the microstructure and mechanical properties of marine 10Ni5CrMoV steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 402-413页

作者： Tao Zou Yanwu dong Zhouhua Jiang Shuyang Du Yushuo Li School of Metallurgy Northeastern UniversityShenyang 110819China Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China Key Laboratory for Ecological Metallurgy of Multimetallic Ores(Ministry of Education) Northeastern UniversityShenyang 110819China

Multistage heat treatment involving quenching(Q),lamellarizing(L),and tempering(T)is applied to marine 10Ni5CrMoV *** microstructure and mechanical properties were studied by multiscale characterizations,and the kinetics of reverse austenite transformation,strain hardening behavior,and toughening mechanism were further *** lamellarized specimens possess low yield strength but high toughness,especially cryogenic *** leads to the development of film-like reversed austenite at the martensite block and lath boundaries,refining the martensite structure and lowering the equivalent grain *** analysis of austenite reversion based on the JMAK model shows that the isothermal transformation is dominated by the growth of reversed austenite,and the maximum transformation of reversed austenite is reached at the peak temperature(750℃).The strain hardening behavior based on the modified Crussard-Jaoul analysis indicates that the reversed austenite obtained from lamellarization reduces the proportion of martensite,significantly hindering crack propagation via martensitic transformation during the *** a consequence,the QLT specimens exhibit high machinability and low yield *** with the QT specimen,the ductile-brittle transition temperature of the QLT specimens decreases from-116 to-130℃due to the low equivalent grain size and reversed austenite,which increases the cleavage force required for crack propagation and absorbs the energy of external load,*** work provides an idea to improve the cryogenic toughness of marine 10Ni5CrMoV steel and lays a theoretical foundation for its industrial application and comprehensive performance improvement.

关键词： 10Ni5CrMoV steel lamellarizing reversed austenite cryogenic toughness ductile-brittle transition temperature

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Low-temperature chlorination roasting technology for the simultaneous recovery of valuable metals from spent LiCoO_(2)cathode material

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 80-91页

作者： Junjie Shi Changle Hou Jingjing dong dong Chen Jianzhong Li Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular *** study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode *** activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded *** conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,*** of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and ***,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure *** technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.

关键词： spent lithium-ion battery thermodynamics chlorination roasting kinetics circular economy

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聚多曲霉菌原生质体和液泡的制备及优化

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基因组学与应用生物学 2021年第4期40卷 1611-1617页

作者：董孟雪邹路易郁红艳王涛王小芳余安江子扬滕跃江南大学环境与土木工程学院无锡214122

原生质体的大量制备是研究原生质体转化、诱变、融合等技术的关键,液泡的制备对研究液泡中分解、转运有机物的特性具有重要意义,但目前分离技术还不够成熟。本研究从聚多曲霉菌菌丝中分离原生质体和液泡并对分离条件进行优化,以聚多曲霉... 详细信息

原生质体的大量制备是研究原生质体转化、诱变、融合等技术的关键,液泡的制备对研究液泡中分解、转运有机物的特性具有重要意义,但目前分离技术还不够成熟。本研究从聚多曲霉菌菌丝中分离原生质体和液泡并对分离条件进行优化,以聚多曲霉菌DJ515-2菌丝为材料,探索不同因素对原生质体和液泡制备分离效果的影响。结果表明,聚多曲霉菌DJ515-2在菌龄42 h,以3%纤维素酶、1%蜗牛酶和3%的溶壁酶组成复合酶液,25℃下酶解4 h,原生质体达到最大产量,为5.167×10^(5)个/mL。同时,在此基础上裂解液泡的最优条件为pH 7.5、1.0 mol/L KCl、0.020%Triton X-10,其产量达2.63×10^(5)个/m L,产率为原生质体的50.8%,相比采用多元碱化合物诱导真菌原生质体裂解释放液泡的产率增加了40%~45%。本研究为真菌和植物的各种原生质体技术及基于亚细胞层面的研究提供了材料基础。

关键词：聚多曲霉菌(Aspergillus sydowii) 原生质体液泡单细胞分析

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