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Noninvasive Tracking of Every Individual in Unmarked Mouse Groups Using Multi-Camera Fusion and Deep Learning

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Neuroscience Bulletin 2023年第6期39卷 893-910页

作者： Feng Su Yangzhen Wang mengping wei Chong Wang Shaoli Wang Lei Yang Jianmin Li Peijiang Yuan Dong-Gen Luo Chen Zhang Department of Neurobiology School of Basic Medical SciencesBeijing Key Laboratory of Neural Regeneration and RepairCapital Medical UniversityBeijing100069China Chinese Institute for Brain Research Beijing102206China State Key Laboratory of Translational Medicine and Innovative Drug Development Nanjing210000China Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing100871China School of Life Sciences Tsinghua UniversityBeijing100084China School of Biological Science and Medical Engineering Beihang UniversityBeijing100191China The Key Laboratory of Developmental Genes and Human Disease Institute of Life SciencesSoutheast UniversityNanjing210096JiangsuChina Institute for Artificial Intelligence the State Key Laboratory of Intelligence Technology and SystemsBeijing National Research Center for Information Science and TechnologyDepartment of Computer Science and TechnologyTsinghua UniversityBeijing100084China School of Mechanical Engineering and Automation Beihang UniversityBeijing100191China

Accurate and efficient methods for identifying and tracking each animal in a group are needed to study complex behaviors and social *** tracking methods(e.g.,marking each animal with dye or surgically implanting microchips)can be invasive and may have an impact on the social behavior being *** overcome these shortcomings,video-based methods for tracking unmarked animals,such as fruit flies and zebrafish,have been ***,tracking individual mice in a group remains a challenging problem because of their flexible body and complicated interaction *** this study,we report the development of a multi-object tracker for mice that uses the Faster region-based convolutional neural network(R-CNN)deep learning algorithm with geometric transformations in combination with multi-camera/multi-image fusion *** system successfully tracked every individual in groups of unmarked mice and was applied to investigate chasing *** proposed system constitutes a step forward in the noninvasive tracking of individual mice engaged in social behavior.

关键词： Noninvasive tracking Deep learning Multi-camera Mouse group Social interaction

Timp1 Deletion Induces Anxiety-like Behavior in Mice

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Neuroscience Bulletin 2024年第6期40卷 732-742页

作者： Xiaotong Wang wei Zheng Ziyi Zhu Biyu Xing weijie Yan Ke Zhu Lingli Xiao Chaojuan Yang mengping wei Lei Yang Zi-Bing Jin Xueyun Bi Chen Zhang State Key Laboratory of Neurology and Oncology Drug Development Nanjing210000China School of Basic Medical Sciences Wenzhou Medical UniversityWenzhou325035China School of Basic Medical Sciences Beijing Key Laboratory of Neural Regeneration and RepairAdvanced Innovation Center for Human Brain ProtectionBeijing Laboratory of Oral HealthCapital Medical UniversityBeijing100069China Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing100005China Chinese Institute for Brain Research Beijing102206China Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Engineering MedicineBeihang UniversityBeijing100191China

The hippocampus is essential for learning and memory,but it also plays an important role in regulating emotional behavior,as hippocampal excitability and plasticity affect anxiety and *** synaptic plasticity may be regulated by tissue inhibitor of matrix metalloproteinase 1(TIMP1),a known protein inhibitor of extracellular matrix(ECM),and the expression of TIMP1 in the hippocampus can be induced by neuronal excitation and various ***,the involvement of Timp1 in fear learning,anxiety,and hippocampal synaptic function remains to be *** study of Timp1 function in vivo revealed that Timp1 knockout mice exhibit anxiety-like behavior but normal fear *** results suggested that Timp1 knockout mice showed hyperactivity in the ventral CA1 region,but the basic synaptic transmission and plasticity were normal in the Schaffer collateral *** together,our results suggest that deletion of Timp1 in vivo leads to the occurrence of anxiety behaviors,but that Timp1 is not crucial for fear learning.

关键词： Timp1 Anxiety Fear Synaptic transmission Synaptic plasticity

Alpha/beta-hydrolase domain-containing 6（ABHD6） negatively regulates the surface delivery and synaptic function of AMPA receptors

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Alpha/beta-hydrolase domain-containing 6（ABHD6） negatively...

Alpha/beta-hydrolase domain-containing 6（ABHD6） negatively regulates the surface delivery and synaptic function of AMPA receptors

中国神经科学学会第十二届全国学术会议

作者： mengping wei Jian Zhang Moye Jia Chaojuan Yang Yunlong Pan Shuaiqi Li Yiwen Luo Junyuan Zheng Jianguo Ji Jianguo Chen Xinli Hu Jingwei Xiong Yun Shi Chen Zhang State Key Laboratory of Membrane Biology School of Life Sciences Peking University–IDG/McGovern Institute for Brain Research Peking University Institute of Molecular Medicine Peking University MOE Key Laboratory of Model Animal for Disease Study Model Animal Research Center of Nanjing University

In the brain, a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid（AMPA）-type glutamate receptors are major postsynaptic receptors at excitatory synapses that mediate fast neurotransmission and synaptic plasticity. α/β-Hydrolase domain containing 6（ABHD6）, a monoacylglycerol lipase, was previously found to be a component of AMPA receptor macromolecular complexes, but its physiological significance in the function of AMPA receptors（AMPARs） has remained unclear. The present study showed that overexpression of ABHD6 in neurons drastically reduced excitatory neurotransmission mediated by AMPA but not by N-methyl-D-aspartate receptors（NMDARs） at excitatory synapses. Inactivation of ABHD6 expression in neurons by either CRISPR/Cas9 or sh RNA knockdown methods significantly increased excitatory neurotransmission at excitatory synapses. Interestingly, overexpression of ABHD6 reduced glutamate-induced currents and the surface expression of Glu A1 in HEK293 T cells expressing Glu A1 and stargazin, suggesting a direct functional interaction between these two proteins. The C-terminal tail of Glu A1 was required for the binding between of ABHD6 and Glu A1. Mutagenesis analysis revealed a GFCLIPQ sequence in the GluA1C-terminus that was essential for the inhibitory effect of ABHD6. The hydrolase activity of ABHD6 was not required for the effects of ABHD6 on AMPAR function in either neurons or transfected HEK293 T cells. Thus, these findings reveal a novel and expected mechanism governing AMPAR trafficking at synapses through ABHD6.

关键词： ABHD6 AMPA receptor synapses receptor trafficking

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核苷(酸)类似物抗病毒治疗对乙型肝炎病毒相关肝细胞癌患者临床特征及预后的影响

第九届海内外华人神经科学家研讨会

作者： mengping wei Jian Zhang Moye Jia Chaojuan Yang Yunlong Pan Shuaiqi Li Yiwen Luo Junyuan Zheng Jianguo Ji Jianguo Chen Xinli Hu Jingwei Xiong Yun Shi Chen Zhang State Key Laboratory of Membrane Biology School of Life Sciences Institute of Molecular Medicine Peking University cMOE Key Laboratory of Model Animal for Disease Study Model Animal Research Center of Nanjing University

In the brain, a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid（AMPA）-type glutamate receptors are major postsynaptic receptors at excitatory synapses that mediate fast neurotransmission and synaptic plasticity. α/β-Hydrolase domain containing 6（ABHD6）, a monoacylglycerol lipase, was previously found to be a component of AMPA receptor macromolecular complexes, but its physiological significance in the function of AMPA receptors（AMPARs） has remained unclear. The present study showed that overexpression of ABHD6 in neurons drastically reduced excitatory neurotransmission mediated by AMPA but not by N-methylD-aspartate receptors（NMDARs） at excitatory synapses. Inactivation of ABHD6 expression in neurons by either CRISPR/Cas9 or sh RNA knockdown methods significantly increased excitatory neurotransmission at excitatory synapses. Interestingly, overexpression of ABHD6 reduced glutamate-induced currents and the surface expression of GluA1 in HEK293 T cells expressing GluA1 and stargazin, suggesting a direct functional interaction between these two proteins. The Cterminal tail of GluA1 was required for the binding between of ABHD6 and GluA1. Mutagenesis analysis revealed a GFCLIPQ sequence in the Glu A1 C-terminus that was essential for the inhibitory effect of ABHD6. The hydrolase activity of ABHD6 was not required for the effects of ABHD6 on AMPAR function in either neurons or transfected HEK293 T cells. Thus, these findings reveal a novel and expected mechanism governing AMPAR trafficking at synapses through ABHD6.

关键词： ABHD6 AMPA receptor synapses receptor trafficking

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中华传染病杂志 2023年第2期41卷 144-151页

作者：魏梦平马路园苏佩华赵彩彦河北医科大学第三医院感染科石家庄050051

目的分析采用核苷(酸)类似物(NAs)抗病毒治疗对乙型肝炎病毒(HBV)相关肝细胞癌(HCC)患者临床特征及预后的影响。方法回顾性分析2015年1月至2021年1月在河北医科大学第三医院首次确诊的450例HBV相关HCC患者的病历资料,其中持续NAs治疗组... 详细信息

目的分析采用核苷(酸)类似物(NAs)抗病毒治疗对乙型肝炎病毒(HBV)相关肝细胞癌(HCC)患者临床特征及预后的影响。方法回顾性分析2015年1月至2021年1月在河北医科大学第三医院首次确诊的450例HBV相关HCC患者的病历资料,其中持续NAs治疗组193例,HCC后NAs治疗组257例,采用倾向评分匹配均衡两组的基线资料。采用Kaplan-Meier法计算两组无复发生存率,采用Cox比例风险回归模型分析HCC复发的危险因素,采用Spearman相关性分析探究持续NAs治疗组患者HCC临床特征与HBV DNA载量的关系。结果匹配前,持续NAs治疗组中有肝硬化、体重指数≥25.0 kg/m2、单发肿瘤、肿瘤最大径≤5 cm、Child-Pugh分级A级、中国肝癌分期方案Ⅰ期的比例分别为93.8%(181/193)、45.1%(87/193)、70.5%(136/193)、82.4%(159/193)、74.6%(144/193)、74.6%(144/193),分别高于HCC后NAs治疗组的87.5%(225/257)、44.0%(113/257)、61.1%(157/257)、55.3%(142/257)、63.8%(164/257)、56.0%(144/257);持续NAs治疗组有饮酒史、发生门静脉癌栓的比例分别为12.4%(24/193)和3.1%(6/193),分别低于HCC后NAs治疗组的33.9%(87/257)和10.5%(27/257);差异均有统计学意义(χ^(2)=4.86、7.58、4.27、36.63、8.15、21.05、27.21、8.88,均P<0.05)。匹配后,持续NAs治疗组和HCC后NAs治疗组患者的中位无复发生存期分别为388 d和277 d,5年累积无复发生存率分别为50.0%和37.5%,差异有统计学意义(χ^(2)=5.30,P=0.021)。多因素分析显示,HCC前未行抗病毒治疗、多发肿瘤、肿瘤最大径≥5 cm、姑息治疗是影响HBV相关HCC复发的独立危险因素(风险比为1.509、1.491、0.446、1.472,均P<0.05)。匹配后,持续NAs治疗组的肿瘤最大径(r=0.175,P=0.042)、发生门静脉癌栓(r=0.210,P=0.014)、HBV相关HCC复发(r=0.178,P=0.038)与HBV DNA载量均呈正相关。结论尽早启动NAs抗病毒治疗能一定程度上改善患者进展至HBV相关HCC的肿瘤学特征,提高无复发生存率,而确诊HCC前未行抗病毒治疗、多发肿瘤、肿瘤最大径≥5 cm、姑息治疗则是影响HBV相关HCC复发的独立危险因素。

关键词：核苷(酸)类似物癌,肝细胞预后

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气相色谱法测定工作场所空气中磷酸三苯酯

中国职业医学 2018年第1期45卷 88-90页

作者：李龙张彪关英俊冯统新邹薇张梦萍卫海燕张志虎济南大学山东省医学科学院医学与生命科学学院山东济南250062 山东省医学科学院山东省职业卫生与职业病防治研究院 250062 陕西未来能源化工有限公司陕西榆林719000

目的建立工作场所空气中磷酸三苯酯(TPP)的气相色谱测定方法。方法工作场所空气中的TPP以玻璃纤维滤纸采样后,经乙醚解吸,HP-5气相色谱柱分离,火焰光度检测器检测。结果 TPP在质量浓度为12.50~800.00 mg/L时线性关系良好,相关系数为0.99... 详细信息

目的建立工作场所空气中磷酸三苯酯(TPP)的气相色谱测定方法。方法工作场所空气中的TPP以玻璃纤维滤纸采样后,经乙醚解吸,HP-5气相色谱柱分离,火焰光度检测器检测。结果 TPP在质量浓度为12.50~800.00 mg/L时线性关系良好,相关系数为0.999 9;方法检出限为0.78 mg/L,最低检出浓度为0.09 mg/m3(以采样45 L计算);解吸效率为97.2%~99.4%;加标回收率为99.5%~100.3%;批内相对标准偏差(RSD)为2.7%~3.4%,批间RSD为1.4%~3.2%;采样效率为99.6%~100.0%。样品在室温下至少可保存14 d。结论本方法简便、准确、灵敏度高,适用于工作场所空气中TPP的测定。

关键词：磷酸三苯酯气相色谱法空气工作场所

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工作场所空气中磷酸三丁酯测定的气相色谱法

中华劳动卫生职业病杂志 2017年第8期35卷 618-621页

作者：李龙邹薇张梦萍卫海燕张彪张志虎济南大学山东省医学科学院医学与生命科学学院 250062 山东省职业卫生与职业病防治研究院

目的建立工作场所空气中磷酸三丁酯的采样和测定方法。方法采用玻璃纤维滤纸采样，乙醚洗脱，气相色谱分离，火焰光度检测器（磷滤波器）检测。结果该方法测定磷酸三丁酯的线性范围为7．2～720．0μg／ml，相关系数为0．99992，方法检... 详细信息

目的建立工作场所空气中磷酸三丁酯的采样和测定方法。方法采用玻璃纤维滤纸采样，乙醚洗脱，气相色谱分离，火焰光度检测器（磷滤波器）检测。结果该方法测定磷酸三丁酯的线性范围为7．2～720．0μg／ml，相关系数为0．99992，方法检出限为0．86μg／ml，最低检出浓度为0．14mg／m^3（以采样空气样品体积30L计算）；加标回收率为99．8％～100．2％，批内精密度为4．0％～5．4％，批间精密度为2．0％。5．5％，采样效率为99．1％～100．0％。结论该方法各项指标均达到GBZ／T210．4—2008（（职业卫生标准制定指南第4部分：工作场所空气中化学物质测定方法》的要求，能准确地测定工作场所空气中磷酸三丁酯含量。

关键词：磷酸三丁酯色谱法,气相工作场所空气

玉米地植保无人机施药对草地贪夜蛾的防治效果研究

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陕西农业科学 2023年第8期69卷 73-77页

作者：潘丽香覃帅韦华艳韦孟萍韦建赳兰加永河池市金城江区农业农村局广西河池547000 河池市发展和改革委员会广西河池547000

为探究防治草地贪夜蛾的有效方法,本次研究以河池市金城江区虫害玉米地为研究对象,使用喷施甲氨基阿维菌素苯甲酸盐微乳剂以及甲氨基阿维菌素苯甲酸盐-金龟子绿僵菌CQMa421混合药剂两种药剂、无人机和人工背负式喷施两种施药方式进行试... 详细信息

为探究防治草地贪夜蛾的有效方法,本次研究以河池市金城江区虫害玉米地为研究对象,使用喷施甲氨基阿维菌素苯甲酸盐微乳剂以及甲氨基阿维菌素苯甲酸盐-金龟子绿僵菌CQMa421混合药剂两种药剂、无人机和人工背负式喷施两种施药方式进行试验。结果表明,人工喷施见效较快,但在喷施10 d后人工和无人机喷药的防治效果均高于94%,且没有显著性差异,无人机喷药成本远低于人工成本;喷施混合药剂的防治效果略高于只喷施甲氨基阿维菌素苯甲酸盐微乳剂。推荐生产实践中采用无人机喷施混合药剂的方式防治玉米地草地贪夜蛾。

关键词：草地贪夜蛾玉米无人机农药防治效果

Mechanism and effect of stress granule formation in cancer and its potential roles in breast cancer therapy

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Genes & Diseases 2022年第3期9卷 659-667页

作者： Taobo Hu wei Hou Enhua Xiao mengping Long Department of Breast Surgery Peking University People’s HospitalBeijing 100044PR China Department of Pathology Peking University Cancer HospitalBeijing 100142PR China Department of Radiology The Second Xiangya HospitalCentral South UniversityChangshaHunan 410011PR China

Stress granules are non-membranous cytoplasmic foci induced by various stress *** is a protective strategy used by cells to suppress overall translation during *** cancer cells,it was thought that the formation of stress granules could protect them from apoptosis and induces resistance towards anti-cancer drugs or radiation treatment which makes the stress granules a potential target for cancer ***,most of our understanding of stress granules are still in the stage of molecular and cell biology,and a transitional gap for its actual effect on clinical settings *** this review,we summarize the mechanism and effect of stress granules formation in cancer and try to illuminate its potential applications in cancer therapy,using breast cancer as an example.

关键词： Apoptosis Breast cancer Drug resistance Stress granules Translation initiation