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Proteomic analysis on cellular response induced by nanoparticles reveals the nano-trafficking pathway through epithelium

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Journal of Chinese Pharmaceutical Sciences 2021年第2期30卷 107-118页

作者： Jian Zhang mengmeng qin Dan Yang Wenbing Dai Hua Zhang Xueqing Wang Bing He Qiang Zhang State Key Luboratoty of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Beijing Key Laboratoty of Molecular Pharmaceutics and New Drug Delivery Systems School of Pharmaceutical SciencesPeking UniversityBeijing 100191China School of Food ami Biological Engineering Shaanxi University of Science and TechnologyXi'an 710021China

The application of nanomedicines in oral drug delivery effectively promotes the drug absorption and transportation through ***,the absence of mechanism studies on efficacy and safety limits their final translation in *** the vesicular trafficking has been verified as the general character for transport of nanomedicines,the deeper mechanism in molecular mechanism is still ***,the cellular transport of nanomedicines is a dynamic process involved by different organelles and ***,most of existing studies just pay attention to the static location of nanomedicines,but neglect the dynamic biological effects on cells caused by ***,we prepared gold nanoparticles(Au NPs)as the model and cultured epithelial cell monolayer to explore the nano-bio interactions at the molecular *** traditional pharmacological inhibition strategy and subcellular imaging technology elucidated the macropinocytosis/endosome/MVB/lysosome pathway during the transportation of Au *** strategy based on mass spectrometry(MS)was utilized to identify and quantify proteins involved in the cellular transport of *** proteins related to subcellular structure,signal transduction,energy transformation and metabolism regulation were demonstrated to be regulated by nanoparticle *** alterations of protein expression clarified the effects of intracellular proteins and verified the conventional *** importantly,it revealed a feedback mechanism of cells to the *** believed that these new regulatory mechanisms provided new insights into the efficient transport of nanomedicines through epithelial barriers.

关键词： Epithelium Gold nanoparticles Intracellular transport Proteomics Cellular response

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体育旅游视角下陈家沟运动休闲特色小镇建设研究

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中华武术（研究） 2019年第4期8卷 46-49页

作者：秦蒙蒙丁保玉天津体育学院体育文化学院天津301617 天津体育学院武术教研室天津301617

本文运用文献资料、田野调查等研究方法,在体育旅游视角下对河南省陈家沟运动休闲特色小镇建设进行深入调查。结论:(1)河南省陈家沟运动休闲特色小镇建设已经初具规模,基本建成以"陈式太极拳文化园""陈家沟水上乐园"和"陈家沟拓展营"为... 详细信息

本文运用文献资料、田野调查等研究方法,在体育旅游视角下对河南省陈家沟运动休闲特色小镇建设进行深入调查。结论:(1)河南省陈家沟运动休闲特色小镇建设已经初具规模,基本建成以"陈式太极拳文化园""陈家沟水上乐园"和"陈家沟拓展营"为特色的运动休闲小镇;(2)由于建设时间不长,一些项目存在场地设施不健全、项目活动单一、组织力度不强等问题。建议:(1)政府健全组织管理制度,加大宣传力度;(2)适当开发当地的体育场地,完善体育器材设施;(3)加强对运动休闲特色的体育管理人才的培养。

关键词：特色小镇体育旅游美丽乡村陈家沟

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DKP-SLAM:A Visual SLAM for Dynamic Indoor Scenes Based on Object Detection and Region Probability

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Computers, Materials & Continua 2025年第1期82卷 1329-1347页

作者： Menglin Yin Yong qin Jiansheng Peng College of Automation Guangxi University of Science and TechnologyLiuzhou545000China Department of Artificial Intelligence and Manufacturing Hechi UniversityHechi546300China Key Laboratory of AI and Information Processing Education Department of Guangxi Zhuang Autonomous RegionHechi546300China Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology School of Chemistry and BioengineeringHechi UniversityHechi546300China

In dynamic scenarios,visual simultaneous localization and mapping(SLAM)algorithms often incorrectly incorporate dynamic points during camera pose computation,leading to reduced accuracy and *** paper presents a dynamic SLAM algorithm that leverages object detection and regional dynamic ***,a parallel thread employs the YOLOX object detectionmodel to gather 2D semantic information and compensate for missed ***,an improved K-means++clustering algorithm clusters bounding box regions,adaptively determining the threshold for extracting dynamic object contours as dynamic points *** process divides the image into low dynamic,suspicious dynamic,and high dynamic *** the tracking thread,the dynamic point removal module assigns dynamic probability weights to the feature points in these *** with geometric methods,it detects and removes the dynamic *** final evaluation on the public TUM RGB-D dataset shows that the proposed dynamic SLAM algorithm surpasses most existing SLAM algorithms,providing better pose estimation accuracy and robustness in dynamic environments.

关键词： Visual SLAM dynamic scene YOLOX K-means++clustering dynamic probability

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Enhancing m^(6)A modification in the motor cortex facilitates corticospinal tract remodeling after spinal cord injury

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Neural Regeneration Research 2025年第6期20卷 1749-1763页

作者： Tian qin Yuxin Jin Yiming qin Feifei Yuan Hongbin Lu Jianzhong Hu Yong Cao Chengjun Li Department of Spine Surgery and Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan ProvinceChangshaHunan ProvinceChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Sports Medicine Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

Spinal cord injury typically causes corticospinal tract disruption. Although the disrupted corticospinal tract can self-regenerate to a certain degree, the underlying mechanism of this process is still unclear. N6-methyladenosine(m^(6)A) modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes. However, whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown. We found that expression of methyltransferase 14 protein(METTL14) in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels. Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury. Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction, we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner, thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration. Finally, we administered syringin, a stabilizer of METTL14, using molecular docking. Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14. Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.

关键词： corticospinal tract remodeling epigenetic regulations locomotor cortex m^(6)A modification methyltransferase 14 protein(METTL14) mitogen-activated protein kinase neural regeneration spinal cord injury syringin TRIB2

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一种微量有效检测小麦面粉筋力的方法

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河南工业大学学报（自然科学版） 2016年第1期37卷 1-4页

作者：贾峰周晓配秦梦梦王金水河南工业大学生物工程学院河南郑州450001

分别用水和乳酸作为溶剂提取小麦面粉中的相关蛋白,根据提取的蛋白含量与面粉筋力之间的相关性,建立初步评定面粉筋力的方法。结果表明：小麦面粉中水溶性蛋白占面粉总质量的比值（Gw）与面粉筋力之间的相关系数达到0.989 7,而酸溶性蛋... 详细信息

分别用水和乳酸作为溶剂提取小麦面粉中的相关蛋白,根据提取的蛋白含量与面粉筋力之间的相关性,建立初步评定面粉筋力的方法。结果表明：小麦面粉中水溶性蛋白占面粉总质量的比值（Gw）与面粉筋力之间的相关系数达到0.989 7,而酸溶性蛋白占面粉总质量的比值与面粉筋力之间的相关系数为0.800 4。小麦面粉中水溶性蛋白占面粉总质量的比值大小分为3段,即当Gw≤0.925时为低筋面粉;0.

关键词：小麦面粉筋力微量简便紫外分光光度法

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Integrated combination delivery of IDO inhibitor and paclitaxel for cancer treatment

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Journal of Chinese Pharmaceutical Sciences 2021年第1期30卷 1-16页

作者： Dakuan Wang Bo Peng mengmeng qin Minghui Li Ge Song Bing He Hua Zhang Wenbing Dai Qiang Zhang Xiangbao Meng Huan Meng Xueqing Wang Beijing Key Laboratoiy of Molecular Pharmaceutics New Drug Delivefy SystemsSchool of Pharmaceutical SciencesPeking UniversityBeijing 100191China Department of Chemical Biology School of Pharmaceutical SciencesPeking UniversityBeijing 100191China State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China

In order to realize the combination of chemotherapy and immunotherapy,a reduction-responsive paclitaxel(PTX)prodrug PEG-SS-PTX was synthesized and used as a carrier to encapsulate IDO inhibitor CY-1-4 for preparing PEG-SS-PTX/CY-1-4 ***-SS-PTX/CY-1-4 NPs were evaluated by cytotoxicity,immunogenic cell death(ICD)induction ability and anti-tumor *** light scattering(DLS)results showed that the size of PEG-SS-PTX/CY-1-4 NPs was about 149 *** vitro experiments indicated that its cytotoxicity was in a concentration-dependent manner,and it induced the ICD of B16-F10 *** vivo studies in melanoma mouse model indicated that PEG-SS-PTX/CY-1-4 NPs significantly inhibited the tumor growth and reduced the expression of IDO in tumor ***,it increased the rate of CD8+T cells in the *** summary,PEG-SS-PTX/CY-1-4 NPs achieved good anti-tumor effects and reduced the dose of chemotherapy drugs,which was a safe and effective combined delivery system.

关键词： Chemotherapy Immunotherapy Combination drug delivery Nano drug delivery system IDO activity inhibition Immunogenic cell death

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lncRNA SNHG4 enhanced gastric cancer progression by modulating miR-409-3p/CREB1 axis

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Oncology Research 2025年第1期33卷 185-198页

作者： ZHOUYANG CHENG YUCHEN HUA YANG CAO JUN qin Department of General Surgery Affiliated Hospital of Nantong UniversityNantong226001China Department of Surgery Affiliated Hospital of Nantong UniversityMedical School of Nantong UniversityNantong226001China Department of Operation Affiliated Hospital of Nantong UniversityNantong226001China

Objective:Gastric cancer(GC)is a globally common cancer characterized by high incidence and mortality *** in the molecular understanding of GC provide promising targets for GC diagnosis and *** non-coding RNAs(lncRNAs)and their downstream regulators are regarded to be implicated in the progression of multiple types of *** have shown that the lncRNA small nucleolar RNA host gene 4(SNHG4)serves as a tumor promoter in various malignancies,while its function in GC has yet to be ***,our study aimed to explore the role and underlying mechanism of SNHG4 in ***:We used qRT-PCR to analyze SNHG4 expression in GC tissues and ***-Meier analysis was used to assess the correlation between SNHG4 expression and the survival rate of GC *** function experiments such as CCK-8,BrdU,colony formation,flow cytometry analysis,and transwell were performed to explore the effects of SNHG4 on GC cell proliferation,apoptosis,cell cycle,migration,and *** also established xenograft mouse models to explore the effect of SNHG4 on GC tumor ***,dual luciferase reporter assay was used to verify the interaction between SNHG4 and miR-409-3p and between miR-409-3p and cAMP responsive element binding protein 1(CREB1).Results:The results indicated that SNHG4 was overexpressed in GC tissues and cell lines,and was linked with poor survival rate of GC ***4 promoted GC cell proliferation,migration,and invasion while inhibiting cell apoptosis and cell cycle arrest in *** in vivo experiment indicated that SNHG4 facilitated GC tumor ***,SNHG4 was demonstrated to bind to ***,CREB1 was directly targeted by *** assays demonstrated that miR-409-3p deficiency reversed the suppressive impact of SNHG4 knockdown on GC cell ***,miR-409-3p was also revealed to inhibit GC cell proliferation,migration,and invasion by targeting CREB1

关键词： Gastric cancer Small nucleolar RNA host gene 4(SNHG4) MicroRNA-409-3p(miR-409-3p) cAMP responsive element binding protein 1(CREB1)

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Post-tunable salt gel electrolytes toward flexible supercapacitor switches

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Journal of Energy Chemistry 2025年第1期100卷 114-124页

作者： Hui Wang Shuang Chen Xuming Liu qin Zhang Xin Liu Polymeric and Soft Materials Laboratory School of Chemistry and Life Science and Advanced Institute of Materials Science Changchun University of Technology Polymeric and Soft Materials Laboratory School of Chemical Engineering and Advanced Institute of Materials Science Changchun University of Technology

Convertible hydrogel supercapacitors have emerged as promising energy storage devices in switches,diodes, and transistors. However, inherent weaknesses in ionic conductivity, mechanical properties,and water retention of hydrogel electrolytes seriously hinder their development. Inspired by the hardness conversion of sea cucumber skin, a conductivity and mechanics dual-tunable salt gel electrolyte is successfully designed. The salt gel presents a reversible switching of conductors-insulators and a mechanical regulation between softness and hardness via the dissolution-crystallization transition of sodium acetate trihydrate（SAT）. Meanwhile, the salt gels spontaneously grow a layer of “armor” through saturated phase-change salt crystals effectively reducing water evaporation of hydrogel ***, this phase-change soft-rigid conversion strategy will expand the capabilities of gel-based flexible supercapacitors(area capacitance: 258.6 mF cm-2), and the capacitance retention rate could still reach 86.9% after 3000 cycles at high temperatures. Moreover, the salt gel supercapacitor is potentially used in over-heat alarm systems. It is anticipated that the strategy of conductivity and mechanics of dual-tunable salt gel would provide a new perspective on the development of energy storage devices,wearable electronics, and flexible robots.

关键词： Capacitance

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Potential role of tanycyte-derived neurogenesis in Alzheimer's disease

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Neural Regeneration Research 2025年第6期20卷 1599-1612页

作者： Guibo Qi Han Tang Jianian Hu Siying Kang Song qin Department of Anatomy Histologyand EmbryologySchool of Basic Medical SciencesFudan UniversityShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghaiChina

Tanycytes, specialized ependymal cells located in the hypothalamus, play a crucial role in the generation of new neurons that contribute to the neural circuits responsible for regulating the systemic energy balance. The precise coordination of the gene networks controlling neurogenesis in naive and mature tanycytes is essential for maintaining homeostasis in adulthood. However, our understanding of the molecular mechanisms and signaling pathways that govern the proliferation and differentiation of tanycytes into neurons remains limited. This article aims to review the recent advancements in research into the mechanisms and functions of tanycyte-derived neurogenesis. Studies employing lineage-tracing techniques have revealed that the neurogenesis specifically originating from tanycytes in the hypothalamus has a compensatory role in neuronal loss and helps maintain energy homeostasis during metabolic diseases. Intriguingly,metabolic disorders are considered early biomarkers of Alzheimer's disease. Furthermore,the neurogenic potential of tanycytes and the state of newborn neurons derived from tanycytes heavily depend on the maintenance of mild microenvironments, which may be disrupted in Alzheimer's disease due to the impaired blood–brain barrier ***, the specific alterations and regulatory mechanisms governing tanycyte-derived neurogenesis in Alzheimer's disease remain unclear. Accumulating evidence suggests that tanycyte-derived neurogenesis might be impaired in Alzheimer's disease, exacerbating neurodegeneration. Confirming this hypothesis, however, poses a challenge because of the lack of long-term tracing and nucleus-specific analyses of newborn neurons in the hypothalamus of patients with Alzheimer's disease. Further research into the molecular mechanisms underlying tanycyte-derived neurogenesis holds promise for identifying small molecules capable of restoring tanycyte proliferation in neurodegenerative diseases. This line of investigation could pro

关键词： Alzheimer's disease blood–brain barrier ependymoglial cells hypothalamus metabolic diseases neural stem cells neurogenesis neuroinflammatory diseases neurons tanycyte

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考试的经济功能新论

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中国考试 2018年第1期 72-78页

作者：胡天佑覃梦蒙湖北师范大学湖北黄石435002

考试在客观上是追求效率的工具和手段,而提升活动效率本身就是发挥了客观的经济功能,科学的考试是一种有效的经济机制。作为获取教育资源的途径,考试赋予应试者一定的教育资格;作为获取社会资源的手段,考试甄别、检测应试者的素质能力,... 详细信息

考试在客观上是追求效率的工具和手段,而提升活动效率本身就是发挥了客观的经济功能,科学的考试是一种有效的经济机制。作为获取教育资源的途径,考试赋予应试者一定的教育资格;作为获取社会资源的手段,考试甄别、检测应试者的素质能力,检验教育投资效果。在人力资本的形成上,考试通过对教育发展的导向和辅助来发挥作用。必须根据人力资源开发的条件和实际需要来设计和应用考试,同时将考试与其他人力资源开发工具相配合,才能真正发挥考试在人力资源开发领域的功能。考试在社会各方面得到广泛应用,考试规模扩大,考试专业化程度提升,考试产业逐步形成,使得关联产业也得到了蓬勃发展,形成了以满足考试主客体双方需要为生存依据的各种产业形态。

关键词：考试社会考试经济考试活动考试的经济功能

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