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Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Frontiers of Environmental Science & Engineering 2019年第3期13卷 115-126页

作者： menglu zhang Sheng Chen Xin Yu Peter Vikesland Amy Pruden Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China University of Chinese Academy of Science Beijing 100049 China Department of Civil and Environmental Engineering Virginia Tech Blacksburg VA 24060 USA

There is a need to improve understanding of the effect of chlorine disinfection on antibiotic resistance genes (ARGs) in order to advance relevant drinking water, wastewater, and reuse treatments. However, few studies have explicitly assessed the physical effects on the DNA. Here we examined the effects of free chlorine (1-20 mg CI2/L) on extracellular genomic, plasmid DNA and select ARGs. Chlorination was found to decrease the fluorometric signal of extracellular genomic and plasmid DNA (ranging from 0.005 to 0.05 μg/mL) by 70%, relative to a no-chlorine control. Resulting DNA was further subject to a fragment analysis using a Bioanalyzer, indicating that chlorination resulted in fragmentation. Moreover, chlorine also effectively deactivated both chromosomal- and plasmidborne ARGs, mecK and tetA., respectively. For concentrations >2 mg CI2//L× 30 min, chlorine efficiently reduced the qPCR signal when the initial concentration of ARGs was 10^5 copies/μL or less. Notably, genomic DNA and mecA gene signals were more readily reduced by chlorine than the plasmid-bome tetK gene (by ~2 fold). Based on the results of qPCR with short (~200 bps) and long amplicons (-1200 bps), chlorination could destroy the integrity of ARGs, which likely reduces the possibility of natural transformation. Overall, our findings strongly illustrate that chlorination could be an effective method for inactivating extracellular chromosomal- and plasmid-bome DNA and ARGs.

关键词： Antibiotic resistance Antibiotic resistance genes (ARGs) Extracellular DNA/ARGs Chlorination

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Control of slope-pattern on the deposition of fan-delta systems:a case study of the Upper Karamay Formation,Junggar Basin

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Frontiers of Earth Science 2024年第2期18卷 384-399页

作者： Mingxuan GAO Xinghe YU Shunli LI Wenmiao zhang Songhao HU menglu zhang School of Energy Resource China University of GeosciencesBeijing 100083China Key laboratory of marine reservoir evolution and hydrocarbon enrichment mechanism(Ministry of Education) China University of GeosciencesBeijing 100083China College of Geosciences China University of PetroleumBeijing 102249China No.1 Oil Production Plant Xinjang Oilfield CompanyKaramay 834099China

The Mesozoic fan deltas in the north-west margin of the Junggar Basin,as important petroleum reservoirs,exhibited complex facies change and internal structures with strong heterogeneity which were controlled by the transformation of slope-patterns,bringing great challenges to the study of sedimentary *** Upper Karamay Formation at north-west margin of the Junggar Basin was the objective in this paper which attempts to clarify the mechanism of sedimentary response and sand-body distribution of fan delta systems under the control of slope-pattern *** on a data set of cores,well logs and seismic,two types of slope-pattern were identified in the study area,which include steep-to-gentle in the south and gentle-to-steep in the *** control of difference slope-patterns on the sand-body distribution was clarified based on the analysis of the sedimentary dynamics,facies characteristics,and depositional evolution of the fan *** study shows that the transport mechanism of sediments on the steep-slope was dominated by debris flows,developing coarse-grained,thick-bedded lobes with poor structural maturity of *** the gentle-slope,the deposition was dominated by hyperconcentrated-traction currents,forming relatively fine-grained,thin-bedded lobes with increased sandy *** sand-bodies show frequent bar-channel transformation and channel down-cutting under the steep slope setting,which exhibit migration of isolated river channels on the gentle *** the steep-to-gentle pattern,the coase-grained sediments were mainly accumulated at slope toe,generally developed equiaxial ***,the coarse-grained clasts were preserved both at proximal and distal lobes on the gentle-to-steep slopes,showing obvious lateral extension of the fan *** slope patterns controlled sedimentary respond rates of the fan deltas during lake level *** comparing the modern cases of fan systems worldwide,the control of slope patterns on deposit

关键词： fan delta slope-pattern sedimentary characteristics the Upper Karamay Formation

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Salinity-oriented environmental flows for keystone species in the Modaomen Estuary, China

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Frontiers of Structural and Civil Engineering 2017年第4期11卷 670-681页

作者： menglu zhang Baoshan CUI Zhiming zhang Xuelian JIANG State Key Laboratory of Environmental Simulation and Pollution Control School of Environment Beijing Normal University Beijing 100875 China

Rapid development and urbanization in recent years have contributed to a reduction in freshwater discharge and intensified saltwater intrusion in the Pearl River Delta. This comprises a significant threat to potable water supplies and overall estuary ecosystem health. In this study, the environmental flows of the Modaomen Estuary, one of the estuaries of the Pear/River Delta in China, were determined based on the salinity demand of keystone species and the linear relationship between river discharge and estuarine salinity. The estimated minimum and optimal annual environmental flows in the Modaomen Estuary were 116.8 ~ I09m3 and 273.8 ~ 109m3, respectively, representing 59.3% and 139.0% of the natural runoff. Water quality assessments in recent years indicate that the environmental flows have not been satisfied most of the time, particularly the optimal environmental flow, despite implementation of various water regulations since 2005. Therefore, water regulations and wetland network recov- eries based on rational environmental flows should be implemented to alleviate saltwater intrusion and for the creation of an ideal estuarine habitat.

关键词： environmental flows keystone species Mod- aomen Estuary river discharge salinity

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Knockout of butyrophilin subfamily 1 member A1(BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells

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Journal of Animal Science and Biotechnology 2020年第4期11卷 975-985页

作者： Liqiang Han menglu zhang Zhiyang Xing Danielle N.Coleman Yusheng Liang Juan J.Loor Guoyu Yang College of Animal Science and Veterinary Medicine Henan Agricultural UniversityZhengzhou 450002PR China Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIllinois 61801USA.

Background: Milk lipids originate from cytoplasmic lipid droplets(LD) that are synthesized and secreted from mammary epithelial cells by a unique membrane-envelopment process. Butyrophilin 1 A1(BTN1 A1) is one of the membrane proteins that surrounds LD, but its role in bovine mammary lipid droplet synthesis and secretion is not well ***: The objective was to knockout BTN1 A1 in bovine mammary epithelial cells(BMEC) via the CRISPR/Cas9 system and evaluate LD formation, abundance of lipogenic enzymes, and content of cell membrane phospholipid(PL) species. Average LD diameter was determined via Oil Red O staining, and profiling of cell membrane phospholipid species via liquid chromatography-tandem mass spectrometry(LC-MS/MS).Results: Lentivirus-mediated infection of the Cas9/sg RNA expression vector into BMEC resulted in production of a homozygous clone BTN1 A1^((-/-)). The LD size and content decreased following BTN1 A1 gene knockout. The m RNA abundance of fatty acid synthase(FASN) and peroxisome proliferator-activated receptor-gamma(PPARG) was downregulated in the BTN1 A1^((-/-))clone. Subcellular analyses indicated that BTN1 A1 and LD were co-localized in the cytoplasm. BTN1 A1 gene knockout increased the percentage of phosphatidylethanolamine(PE) and decreased phosphatidylcholine(PC), which resulted in a lower PC/PE ***: Results suggest that BTN1 A1 plays an important role in regulating LD synthesis via a mechanism involving membrane phospholipid composition.

关键词： Lipid droplet Mammary epithelial cell Milk fat globule Phospholipid

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欧债危机对中国经济的影响——基于欧债危机演进测度的研究

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武汉大学学报（哲学社会科学版） 2015年第3期68卷 80-85页

作者：张梦露吴凤武汉大学经济与管理学院湖北武汉430072

欧洲主权债务危机通过贸易渠道、金融渠道以及非接触途径向中国传导。欧债危机对中国经济影响的实证研究结果显示:欧债危机对中国宏观经济存在一定程度的负面影响。其中出口贸易最先受到直接影响,人民币实际有效汇率上升,金融机构所受... 详细信息

欧洲主权债务危机通过贸易渠道、金融渠道以及非接触途径向中国传导。欧债危机对中国经济影响的实证研究结果显示:欧债危机对中国宏观经济存在一定程度的负面影响。其中出口贸易最先受到直接影响,人民币实际有效汇率上升,金融机构所受影响较小。基于此结论,我国需要调整经济结构、适当扩大内需;推进人民币跨境结算业务、加速人民币国际化进程;实现外汇储备多元化、保障巨额外汇储备安全;加强金融市场建设、健全银行业监管机制。

关键词：欧债危机危机测度共同因素中国经济

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Paget's disease of the nipple in males:two case reports and literature review

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Journal of Bio-X Research 2018年第2期1卷 89-94页

作者： Jie Wang Jiaqi Liu Zeyu Xing Xiangzhi Meng menglu zhang Xin Wang Xiang Wang Department of Breast Surgical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Male breast cancer acounts for less than 0.5%of all breast cancer,and Paget's disease of the breast in males is extremely ***,we report 2 cases of Paget's disease of the nipple areola complex with invasive ductal carcinoma as typical examples of male *** 1 was a 64-year old man with an altered appearance of the left nipple,itching and redness,without a palpable breast mass at *** 2 was a 55-year-old man with a palpable mass in the left breast and histologically confirmed Paget'sdisease of the nipple with invasive ductal *** patients underwent modified radical mastectomy but with different adjuvant therapies and remained well during follow-up with no ***,we reviewed all the sporadic cases of male PDB from the *** may help contribute to the development of diagnostic strategies and appropriate interventions for male Paget's disease of the breast.

关键词： Paget s disease male clinical manifestations treatment prognosis

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获得性再生障碍性贫血体细胞突变及其意义

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中国医学科学院学报 2022年第3期44卷 491-496页

作者：张梦露陈婉淑韩冰中国医学科学院北京协和医学院北京协和医院血液内科北京100730

再生障碍性贫血(AA)与骨髓增生异常综合征(MDS)都是造血干细胞(HSC)异常的克隆性疾病,两者有时难以区分。随着分子检测技术的进步,人们对两者发病机制的认识也在不断深入。本文将综述AA体细胞突变(SM)与细胞遗传学改变特点,分析其与MDS... 详细信息

再生障碍性贫血(AA)与骨髓增生异常综合征(MDS)都是造血干细胞(HSC)异常的克隆性疾病,两者有时难以区分。随着分子检测技术的进步,人们对两者发病机制的认识也在不断深入。本文将综述AA体细胞突变(SM)与细胞遗传学改变特点,分析其与MDS的分子联系以及在疾病转化中的作用。AA中最常见的体细胞性改变是PIGA和人类白细胞抗原(HLA)等位基因的缺失,它们及8三体、del(13q)与AA免疫发病机制相关。AA最常见的5个突变中,PIGA和BCOR/BCORL1突变预后更好,而DNMT3A和ASXL1突变与克隆演变和预后不良相关。AA继发MDS的危险因素包括预后不良的SM和细胞遗传学改变[如del(7q)]、疾病持续时间较长、AA发病年龄、端粒磨损等。因SM动态变化及意义未确定,其在疾病进展中的意义仍不明,但监测SM并结合临床评估预后仍可能指导治疗。

关键词：获得性再生障碍性贫血骨髓异常增生综合征体细胞突变细胞遗传学改变分子发病机制疾病转化

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骨髓增生异常综合征和急性髓系白血病的基因突变比较

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中华血液学杂志 2021年第2期42卷 171-176页

作者：陈婉淑张梦露韩冰中国医学科学院北京协和医院血液科 100730

骨髓增生异常综合征(MDS)是一种起源于造血干细胞的异质性恶性克隆性疾病,以造血功能衰竭、病态造血、高风险向急性髓系白血病(AML)转化为特点。约30%的MDS会转化为AML,24%的患者死于白血病转化[1]。MDS主要突变靶点是与DNA甲基化、染... 详细信息

骨髓增生异常综合征(MDS)是一种起源于造血干细胞的异质性恶性克隆性疾病,以造血功能衰竭、病态造血、高风险向急性髓系白血病(AML)转化为特点。约30%的MDS会转化为AML,24%的患者死于白血病转化[1]。MDS主要突变靶点是与DNA甲基化、染色质修饰、RNA剪接、转录因子、信号转导、黏连蛋白和DNA修复有关的基因,有些突变与AML的驱动突变重叠,提示两者可能拥有共同的克隆起源;另一些突变则在疾病进展时出现,与疾病的最终转归密切相关[2]。因此,比较MDS和AML基因突变谱的异同,将有助于我们在基因水平上了解MDS和AML的发病机制及转化过程,对临床的诊断、治疗有重要意义。本文我们复习了近期国内外多篇探讨MDS和AML基因突变谱的文献,总结MDS和AML基因突变谱在诊断、治疗和预后评估中的意义。

关键词：骨髓增生异常综合征急性髓系白血病病态造血恶性克隆性疾病疾病进展造血干细胞基因突变 DNA甲基化

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基于因子分析法的湖北省制造业技术创新能力评价研究

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科技进步与对策 2011年第18期28卷 123-125页

作者：张梦露张忠家武汉理工大学湖北武汉430070

制造业是一个国家工业经济的基础,其技术创新能力反映了一个地区的经济综合实力。从技术创新的资金投入、人力投入、创新产出等方面构建了湖北省制造业技术创新能力评价指标体系,并用因子分析法对湖北省制造业的技术创新能力进行了实证... 详细信息

制造业是一个国家工业经济的基础,其技术创新能力反映了一个地区的经济综合实力。从技术创新的资金投入、人力投入、创新产出等方面构建了湖北省制造业技术创新能力评价指标体系,并用因子分析法对湖北省制造业的技术创新能力进行了实证研究,最后提出了相关建议。

关键词：制造业技术创新能力因子分析评价研究

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环孢素联合重组人促红细胞生成素治疗慢性再生障碍性贫血的疗效评价

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中国医学科学院学报 2021年第5期43卷 736-742页

作者：陈婉淑张梦露韩冰中国医学科学院北京协和医学院北京协和医院血液科北京100730

目的比较环孢素(CsA)联合重组人促红细胞生成素(rhEPO)和单用CsA治疗慢性再生障碍性贫血(CAA)的疗效和安全性。方法回顾性分析2016年1月至2018年6月北京协和医院血液科收治的79例初治CAA患者的临床资料,其中45例采用CsA+rhEPO治疗,34例... 详细信息

目的比较环孢素(CsA)联合重组人促红细胞生成素(rhEPO)和单用CsA治疗慢性再生障碍性贫血(CAA)的疗效和安全性。方法回顾性分析2016年1月至2018年6月北京协和医院血液科收治的79例初治CAA患者的临床资料,其中45例采用CsA+rhEPO治疗,34例仅采用CsA治疗。入选患者使用CsA至少1.5~2.0年,并随访至少1.0年。比较CsA+rhEPO组和CsA组患者的疗效、不良反应和长期转归,分析可能的疗效影响因素。结果CsA+rhEPO组45例患者中,男14例,女31例,中位年龄43(19,73)岁;CsA使用的中位时间为26(12,38)个月,rhEPO使用的中位时间为4(3,6)个月,中位随访时间为24(12,42)个月。CsA组34例患者中,男16例,女18例,中位年龄36(16,85)岁;CsA使用的中位时间为24(12,40)个月,中位随访时间为25(12,40)个月。两组患者治疗前各临床特征比较差异均无统计学意义(P均>0.05)。治疗后6个月,CsA+rhEPO组患者的部分缓解(PR)(53.3%比25.0%;χ^(2)=6.181,P=0.019)和总反应(OR)(55.6%比31.3%;χ^(2)=4.456,P=0.040)均明显高于CsA组,两组的完全缓解(CR)差异无统计学意义(2.2%比6.3%;χ^(2)=0.810,P=0.567);CsA+rhEPO组和CsA组患者在治疗后3、12个月和随访期末的CR、PR及OR差异均无统计学意义(P均>0.05)。CsA+rhEPO组患者在治疗6个月时的血红蛋白水平明显高于CsA组[(102.6±24.0)g/L比(90.3±29.1)g/L;t=2.017,P=0.047],在治疗3、12个月及随访期末与CsA组差异均无统计学意义(P均>0.05)。rhEPO组11.1%(5/45)的患者出现注射部位疼痛,两组患者在肌酐升高、胆红素轻度升高、转氨酶升高、牙龈轻中度增生、胃肠道反应和肌颤等不良反应方面差异均无统计学意义(P均>0.05)。在随访期内,两组患者的克隆演变率相仿,均无死亡病例。未发现影响rhEPO疗效的临床预测因素。结论与CsA单药治疗相比,CsA联合rhEPO治疗CAA在6个月时有更高的有效率及血红蛋白水平,两组不良反应无明显差别。

关键词：慢性再生障碍性贫血重组人促红细胞生成素环孢素疗效不良反应

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