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Freezing-directed construction of enzyme/nano interfaces:Reagentless conjugation,superior activity,and better stability

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Chinese Chemical Letters 2024年第1期35卷 471-474页

作者： Ke Quan Jiajie Tong lifang Chen Shuyao Fang mengjiao li linlin Wu Zhihe Qing Hunan Provincial Key Laboratory of Cytochemistry School of Food and BioengineeringSchool of Chemistry and Chemical EngineeringChangsha University of Science and TechnologyChangsha 410114China Department of Oncology Tengzhou Central People’s Hospital Affiliated Xuzhou Medical UniversityTengzhou 277500China

Immobilizing enzyme to nano interfaces has demonstrated to be a favorable strategy for prompting the industrialized application of enzyme.Despite tremendous endeavor has been devoted to using gold nanoparticles(AuNPs)as conjugation matrix due to its fascinating physico-chemical properties,maintaining enzymatic activity while circumventing cumbersome modification remains a formidable challenge.Herein,the freezing-directed conjugation of enzyme/nano interfaces was constructed without extra reagent.As the proof of concept,glucose oxidase(GOx)was chosen as model enzyme.The one-pot conjugation process can be facilely completed at−20°C under aqueous solution.Moreover,with the loading of GOx on AuNP at freezing,the enzyme exhibited superior catalytic activity and stability upon thermal and pH perturbation.The mechanism of boosted activity was then discussed in detail.It was found that higher loading density under freezing condition and more enzyme tending to bind AuNPs via Au-S bond were the main factors for the superior activity.More importantly,this methodology was universal and can also be applied to other enzyme which contains natural cysteine,such as horseradish peroxidase(HRP)and papain.This facile conjugation strategy accompanied by remarkable bioactivity expand the possibilities for enzymatic biosensing,microdevice and even drug delivery.

关键词： Enzyme conjugation Freezing AuNPs Catalytic activity

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The evolution and diurnal expression patterns of photosynthetic pathway genes of the invasive alien weed,Mikania micrantha

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Journal of Integrative Agriculture 2024年第2期23卷 590-604页

作者： Kangkang Wang mengjiao Jin Jingjing li Yesong Ren Zaiyuan li Xinghai Ren Cong Huang Fanghao Wan Wanqiang Qian Bo liu Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agriculture/Genome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China Shenzhen Research Institute of Henan University Shenzhen 518000China

Mikania micrantha is a fast-growing global invasive weed species that causes severe damage to natural ecosystems and very large economic losses of forest and crop production.It has advantages in photosynthesis,including a similar net photosynthetic rate as C4 plants and a higher carbon fixation capacity.We used a combination of genomics and transcriptomics approaches to study the evolutionary mechanisms and circadian expression patterns of M.micrantha.In M.micrantha,16 positive selection genes focused on photoreaction and utilization of photoassimilates.In different tissues,98.1%of the genes associated with photoresponse had high expression in stems,and more than half of the genes of the C4 cycle had higher expression in stems than in leaves.In stomatal opening and closing,2 genes of carbonic anhydrase(CAs)had higher expression at 18:00 than at 8:00,and the slow anion channel 1(SLAC1)and high-leaf-temperature 1 kinase(HT1)genes were expressed at low levels at 18:00.In addition,genes associated with photosynthesis had higher expression levels at 7:00 and 17:00.We hypothesized that M.micrantha may undergo photosynthesis in the stem and flower organs and that some stomata of the leaves were opening at night by CO_(2)signals.In addition,its evolution may attenuate photoinhibition at high light intensities,and enhance more efficient of photosynthesis during low light intensity.And the tissue-specific photosynthetic types and different diurnal pattern of photosynthetic-related genes may contribute to its rapid colonization of new habitats of M.micrantha.

关键词： invasion plant Mikania micrantha photosynthesis stomata stem

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Sequential pyrolysis for understanding specific influence of cellulose- and lignin-derived volatiles on properties of counterpart char

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Green Chemical Engineering 2024年第2期5卷 222-235页

作者： Yuewen Shao Chao li mengjiao Fan Guoming Gao Stelgen Inkoua lijun Zhang Shu Zhang Jun Xiang Song Hu Xun Hu School of Material Science and Engineering University of JinanJinan250022China Joint International Research Laboratory of Biomass Energy and Materials College of Materials Science and EngineeringNanjing Forestry UniversityNanjing210037China State Key Laboratory of Coal Combustion Huazhong University of Science and Technology430074China

Interactions of cellulose-and lignin-derived intermediates have been well documented during pyrolysis of lignocellulosic biomass.The reaction network for the interactions is rather complex,as cellulose-derived volatiles could interact/react with not only lignin-derived volatiles but also lignin-derived char and vice versa.To probe specifically the impacts of cellulose-derived volatiles on the lignin-derived char or the opposite,herein the sequential pyrolysis was performed by arranging cellulose in the upper bed with lignin in the lower bed or lignin above with cellulose below at 350 and 650℃,respectively.The results indicated that the sequential pyrolysis of cellulose→lignin or lignin→cellulose at 350℃induced increased char yield from formation of carbonaceous deposits via volatiles-char interactions.Compared with the lignin-derived volatiles,the cellulose-derived volatiles,especially aldehyde fractions,were more reactive towards the lignin-derived char at 350℃,forming oxygen-rich lignin-derived char with a higher yield,an abundance of aliphatic structures and consequently lower thermal stability.In sequential pyrolysis of lignin→cellulose,more aromatics-rich species were deposited on cellulose-derived char,but the lignin-derived volatiles were less reactive for enhancing the char yield.At 650℃,instead of polymerisation of the volatiles on the char,either the cellulose-or lignin-derived char catalyzed the cracking of the counterpart volatiles to remove the aliphatic functionalities,which made the char more aromatic and thermally more stable.

关键词： Cellulose lignin Sequential pyrolysis Char Interaction

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Role-Based Network Embedding via Quantum Walk with Weighted Features Fusion

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Computers, Materials & Continua 2023年第8期76卷 2443-2460页

作者： Mingqiang Zhou mengjiao li Zhiyuan Qian Kunpeng li College of Computer Science Chongqing UniversityChongqing400044China

Role-based network embedding aims to embed role-similar nodes into a similar embedding space,which is widely used in graph mining tasks such as role classification and detection.Roles are sets of nodes in graph networks with similar structural patterns and functions.However,the rolesimilar nodes may be far away or even disconnected from each other.Meanwhile,the neighborhood node features and noise also affect the result of the role-based network embedding,which are also challenges of current network embedding work.In this paper,we propose a Role-based network Embedding via Quantum walk with weighted Features fusion(REQF),which simultaneously considers the influence of global and local role information,node features,and noise.Firstly,we capture the global role information of nodes via quantum walk based on its superposition property which emphasizes the local role information via biased quantum walk.Secondly,we utilize the quantum walkweighted characteristic function to extract and fuse features of nodes and their neighborhood by different distributions which contain role information implicitly.Finally,we leverage the Variational Auto-Encoder(VAE)to reduce the effect of noise.We conduct extensive experiments on seven real-world datasets,and the results show that REQF is more effective at capturing role information in the network,which outperforms the best baseline by up to 14.6% in role classification,and 23% in role detection on average.

关键词： Role-based network embedding quantum walk quantum walk weighted characteristic function complex networks

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Salt-assisted vapor-liquid-solid growth of high-quality ultrathin nickel oxide flakes for artificial synapses in image recognition applications

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Nano Research 2024年第5期17卷 4622-4630页

作者： Hui Zhang Zongjie Shen Alei li lin Wang Qinan Wang Yunfei li Yunlei Zhong Juntong Zhu Yong Zhang mengjiao Han Dan Tian Chun Zhao lixing Kang Qingwen li School of Physical Science and Technology Shanghai Tech UniversityShanghai 201210China Division of Advanced Materials Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123China College of Energy Soochow Institute for Energy and Materials Innovationsand Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu ProvinceSoochow UniversitySuzhou 215123China Songshan Lake Materials Laboratory Dongguan 523808China School of Advanced Technology Xi’an Jiaotong-Liverpool UniversitySuzhou 215123China College of Materials Science and Engineering Co-Innovation Center of Efficient Processing and Utilization of Forest ResourcesNanjing Forestry UniversityNanjing 210037China

Transition metal oxides have attracted intense interest owing to their abundant physical and chemical properties.The controlled preparation of large-area,high-quality two-dimensional crystals is essential for revealing their inherent properties and realizing high-performance devices.However,fabricating two-dimensional(2D)transition metal oxides using a general approach still presents substantial challenges.Herein,we successfully achieve highly crystalline nickel oxide(NiO)flakes with a thickness as thin as 3.3 nm through the salt-assisted vapor-liquid-solid(VLS)growth method,which demonstrated exceptional stability under ambient conditions.To explore the great potential of the NiO crystal in this work,an artificial synapse based on the NiO-flake resistive switching(RS)layer is investigated.Short-term and long-term synaptic behaviors are obtained with external stimuli.The artificial synaptic performance provides the foundation of the neuromorphic application,including handwriting number recognition based on artificial neuron network(ANN)and the virtually unsupervised learning capability based on generative adversarial network(GAN).This pioneering work not only paves new paths for the synthesis of 2D oxides in the future but also demonstrates the substantial potential of oxides in the field of neuromorphic computing.

关键词： nickel oxide(NiO)flakes vapor-liquid-solid(VLS)growth memristor synapse generative adversarial network(GAN)

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Enriched electrophilic oxygen species facilitate acidic oxygen evolution on Ru-Mo binary oxide catalysts

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Nano Research 2024年第3期17卷 1165-1172页

作者： Yaya Wang Yunzhou Wen Yumeng Cheng Xinhong Chen mengjiao Zhuansun Tongbao Wang Jun li Debora Meira Huarui Sun Jun Wei Jia Zhou Yuhang Wang Sisi He Shenzhen Key Laboratory of Flexible Printed Electronics Technology Harbin Institute of Technology(Shenzhen)University TownShenzhen 518055China School of Science Harbin Institute of Technology(Shenzhen)University TownShenzhen 518055China Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory of Advanced Negative Carbon TechnologiesSoochow UniversitySuzhou 215123China Frontiers Science Center for Transformative Molecules Shanghai Jiao Tong UniversityShanghai 200240China Science Division Canadian Light SourceSaskatoonSKS7N 2V3Canada Photon Science Division Argonne National LaboratoryLemontIL60439USA School of Materials Science and Engineering Harbin Institute of Technology(Shenzhen)University TownShenzhen 518055China

The polymer electrolyte membrane(PEM)electrolyzers are burdened with costly iridium(Ir)-based catalysts and high operation overpotentials for the oxygen evolution reaction(OER).The development of earth-abundant,highly active,and durable electrocatalysts to replace Ir is a critical step in reducing the cost of green hydrogen production.Here we develop a Ru5Mo4Ox binary oxide catalyst that exhibits high activity and stability in acidic OER.The electron-withdrawing property of Mo enriches the electrophilic surface oxygen species,which promotes acidic OER to proceed via the adsorbate evolution pathway.As a result,we achieve a 189 mV overpotential at 10 mA·cm^(-2) and a Tafel slope of 48.8 mV·dec^(-1).Our catalyst demonstrates a substantial 18-fold increase in intrinsic activity,as evaluated by turnover frequency,compared to commercially available RuO_(2) and IrO_(2) catalysts.Moreover,we report a stable OER operation at 10 mA·cm^(-2) for 100 h with a low degradation rate of 2.05 mV·h^(-1).

关键词： electrocatalysis oxygen evolution reaction(OER) ruthenium(Ru)-based oxide molybdenum electrophilic oxygen

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TaNRT2.1-6B is a dual-affinity nitrate transporter contributing to nitrogen uptake in bread wheat under both nitrogen deficiency and sufficiency

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The Crop Journal 2022年第4期10卷 993-1005页

作者： mengjiao li Tian Wang Hui Zhang Shuo liu Wenhu li Salah F.Abou Elwafa Hui Tian Key Laboratory of Plant Nutrition and Agri-environment in Northwest China Ministry of Agriculture and Rural AffairsCollege of Natural Resources and EnvironmentNorthwest A&F UniversityYangling 712100ShaanxiChina Agronomy Department Faculty of AgricultureAssiut UniversityAssiut 71526Egypt

Multiple nitrate transporter(NRT)genes exist in the genome of bread wheat,and it is of great importance to identify the elite NRT genes for N-efficient wheat cultivar breeding.A candidate gene association study(CGAS)of six N use efficiency(NUE)related traits(grain N concentration(GNC),straw N concentration(SNC),grain yield(GY),grain N accumulation(GNA),shoot total N accumulation(STN)and N harvest index(NHI))was performed based on SNPs in 46 NRT2 genes using a panel composed of 286 wheat cultivars.CGAS identified TaNRT2.1-6B as an elite NRT gene that is significantly associated with four(NHI,SNC,GNA and GY)of the six NUE-related traits simultaneously.TaNRT2.1-6B is located on the plasma membrane and acts as a dual-affinity NRT.The overexpression of TaNRT2.1-6B increased the N influx and root growth of wheat,whereas gene silence lines resulted in the opposite effects.The overexpression of TaNRT2.1-6B also improved GY and N accumulation of wheat under either limited or sufficient N conditions.The data provide the TaNRT2.1-6B gene and the two associated SNP markers as promising powerful tools for breeding wheat cultivars with high N uptake ability and NUE.

关键词： Candidate gene association study Wheat TaNRT2.1-6B Dual-affinity transporter Nitrogen use efficiency

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Potential benefit of high-dose intravenous vitamin C for coronavirus disease 2019 pneumonia

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Chinese Medical Journal 2022年第1期135卷 23-25页

作者： Bing Zhao mengjiao li Yun ling Yibing Peng Jun Huang Hongping Qu Yuan Gao Yingchuan li Bijie Hu Shuihua Lu Hongzhou Lu Wenhong Zhang Enqiang Mao Department of Emergency Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Infectious Disease Shanghai Public Health Clinical CenterShanghai 201508China Department of Laboratory Medicine Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 200025China Shanghai Institute of Hypertension Shanghai 200025China Department of Critical Care Medicine Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Critical Care Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200217China Department of Critical Care Medicine The Sixth People's HospitalShanghai Jiao Tong UniversityShanghai 200233China Department of Infectious Disease Zhongshan HospitalFudan UniversityShanghai 200032China Tuberculosis Department of Shanghai Public Health Clinical Center Shanghai 201508China Department of Infectious Disease of Shanghai Huashan Hospital Fudan UniversityShanghai 200040China

The coronavirus disease 2019(COVID-19)has been declared as a pandemic by the World Health Organization.[1]Most COVID-19 patients exhibit mild to moderate symptoms,while approximately 15%progress rapidly to severe pneumonia,and about 5%eventually develop acute respiratory distress syndrome(ARDS),[2]which requires mechanical ventilation(MV)and even extracorporeal membrane oxygenation.The mortality of COVID-19 patients who received MV was reported to be as high as 66%.[3]Therefore,the treatments aiming to improve mortality should focus on two aspects:first,prevention of the aggravation of the disease in mild and moderate COVID-19 patients;second,the rescue therapy for patients in serious conditions.We have been applying high-dose intravenous vitamin C(HDIVC)in the treatment of critical illnesses for almost 10 years in our center.Our previous in vivo research showed that HDIVC protected hemorrhagic shock-related multiple organ failure(MOF)by inhibiting inflammatory cytokines and oxidative indicators through activating Sirtuin1 pathway.

关键词： vitamin pneumonia prevention

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论先秦文献中时间概念的多重隐喻

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河南师范大学学报（哲学社会科学版） 2024年第3期51卷 129-135页

作者：钱建成李梦姣郑州大学外国语与国际关系学院河南郑州450001 郑州大学文学院河南郑州450001

中国人自古以来就善于从生活中的事物入手,用具体普遍的事物来表达不可言说的抽象概念。在认知能力有限的条件下,中国早期先民借助空间方位、客观实体实现了对抽象时间概念的具体化表达,并逐渐内化为人们对时间的自觉认知,这一实现过程... 详细信息

中国人自古以来就善于从生活中的事物入手,用具体普遍的事物来表达不可言说的抽象概念。在认知能力有限的条件下,中国早期先民借助空间方位、客观实体实现了对抽象时间概念的具体化表达,并逐渐内化为人们对时间的自觉认知,这一实现过程正是隐喻作为思维方式的结果。先秦文献中对时间的隐喻主要包含对动态时间推移的表达,以及对静态历法、时令、四季节气的描述,形成“时间是空间”与“时间是客观实体”两个根本性隐喻。

关键词：概念隐喻空间方位客观实体隐喻思维

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Applications of laboratory findings in the prevention,diagnosis,treatment,and monitoring of COVID-19

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Signal Transduction and Targeted Therapy 2021年第9期6卷 2697-2722页

作者： Zirui Meng Shuo Guo Yanbing Zhou mengjiao li Minjin Wang Binwu Ying Department of Laboratory Medicine West China HospitalSichuan UniversityChengduSichuan ProvinceChina

The worldwide pandemic of coronavirus disease 2019(COVID-19)presents us with a serious public health crisis.To combat the virus and slow its spread,wider testing is essential.There is a need for more sensitive,specific,and convenient detection methods of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Advanced detection can greatly improve the ability and accuracy of the clinical diagnosis of COVID-19,which is conducive to the early suitable treatment and supports precise prophylaxis.In this article,we combine and present the latest laboratory diagnostic technologies and methods for SARS-CoV-2 to identify the technical characteristics,considerations,biosafety requirements,common problems with testing and interpretation of results,and coping strategies of commonly used testing methods.We highlight the gaps in current diagnostic capacity and propose potential solutions to provide cutting-edge technical support to achieve a more precise diagnosis,treatment,and prevention of COVID-19 and to overcome the difficulties with the normalization of epidemic prevention and control.

关键词： prevention diagnosis treatment

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