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Distinguished biological adaptation architecture aggravated population differentiation of Tibeto-Burman-speaking people

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Journal of Genetics and Genomics 2024年第5期51卷 517-530页

作者： Yuntao Sun mengge wang Qiuxia Sun Yan Liu Shuhan Duan Zhiyong wang Yunyu Zhou Jun Zhong Yuguo Huang Xinyu Huang Qingxin Yang Xiangping Li Haoran Su Yan Cai Xiucheng Jiang Jing Chen Jiangwei Yan Shengjie Nie Liping Hu Junbao Yang Renkuan Tang Chuan-Chao wang Chao Liu Xiaohui Deng Libing Yun Guanglin He West China School of Basic Science&Forensic Medicine Sichuan UniversityChengduSichuan 610041China Institute of Rare Diseases West China Hospital of Sichuan UniversitySichuan UniversityChengduSichuan 610000China Center for Archaeological Science Sichuan UniversityChengduSichuan 610000China Faculty of Forensic Medicine Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdong 510275China Guangzhou Forensic Science Institute GuangzhouGuangdong 510055China Department of Forensic Medicine College of Basic MedicineChongqing Medical UniversityChongqing 400331China School of Clinical Medical Sciences North Sichuan Medical CollegeNanchongSichuan 637100China School of Basic Medical Sciences North Sichuan Medical CollegeNanchongSichuan 637100China School of Forensic Medicine Kunming Medical UniversityKunmingYunnan 650500China School of Stomatology North Sichuan Medical CollegeNanchongSichuan 637100China Department of Medical Laboratory North Sichuan Medical CollegeNanchongSichuan 637007China School of Life Sciences Xiamen UniversityXiamenFujian 361005China Anti-Drug Technology Center of Guangdong Province GuangzhouGuangdong 510230China School of Forensic Medicine Shanxi Medical UniversityJinzhongShanxi030600China

Tibeto-Burman(TB)people have endeavored to adapt to the hypoxic,cold,and high-UV high-altitude environments in the Tibetan Plateau and complex disease exposures in lowland rainforests since the late Paleolithic period.However,the full landscape of genetic history and biological adaptation of geographically diverse TB-speaking people,as well as their interaction mechanism,remain unknown.Here,we generate a whole-genome meta-database of 500 individuals from 39 TB-speaking populations and present a comprehensive landscape of genetic diversity,admixture history,and differentiated adaptative features of geographically different TB-speaking people.We identify genetic differentiation related to geography and language among TB-speaking people,consistent with their differentiated admixture process with incoming or indigenous ancestral source populations.A robust genetic connection between the Tibetan-Yi corridor and the ancient Yellow River people supports their Northern China origin hypothesis.We finally report substructure-related differentiated biological adaptative signatures between highland Tibetans and Loloish speakers.Adaptative signatures associated with the physical pigmentation(EDAR and SLC24A5)and metabolism(ALDH9A1)are identified in Loloish people,which differed from the high-altitude adaptative genetic architecture in Tibetan.TB-related genomic resources provide new insights into the genetic basis of biological adaptation and better reference for the anthropologically informed sampling design in biomedical and genomic cohort research.

关键词： Tibeto-Burman people Geneticprofile Natural selection Geneticdiversity Biological adaptation

An engineered Cas12i nuclease that is an efficient genome editing tool in animals and plants

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The Innovation 2024年第2期5卷 61-69页

作者： Zhiqiang Duan Yafeng Liang Jialei Sun Hongjin Zheng Tong Lin Pengyu Luo mengge wang Ruiheng Liu Ying Chen Shuhua Guo Nannan Jia Hongtao Xie Meili Zhou Minghui Xia Kaijun Zhao Shuhui wang Na Liu Yongling Jia Wei Si Qitong Chen Yechun Hong Ruilin Tian Jian-Kang Zhu Institute of Advanced Biotechnology and School of Medicine Southern University of Science and TechnologyShenzhen 518055China Bellagen Biotechnology Jinan 250000China Department of Medical Neuroscience School of MedicineKey University Laboratory of Metabolism and Health of GuangdongSouthern University of Science and TechnologyShenzhenGuangdong Province 518055China

The type V-I CRISPR-Cas system is becoming increasingly more attractive for genome editing.However,natural nucleases of this system often exhibit low efficiency,limiting their application.Here,we used structure-guided rational design and protein engineering to optimize an uncharacterized Cas12i nuclease,Cas12i3.As a result,we developed Cas-SF01,a Cas12i3 variant that exhibits significantly improved gene editing activity in mammalian cells.Cas-SF01 shows comparable or superior editing performance compared to SpCas9 and other Cas12 nucleases.Compared to natural Cas12i3,Cas-SF01 has an expanded PAM range and effectively recognizes NTTN and noncanonical NATN and TTVN PAMs.In addition,we identified an amino acid substitution,D876R,that markedly reduced the off-target effect while maintaining high on-target activity,leading to the development of CasSF01^(HiFi)(high-fidelity Cas-SF01).Finally,we show that Cas-SF01 has high gene editing activities in mice and plants.Our results suggest that CasSF01 can serve as a robust gene editing platform with high efficiency and specificity for genome editing applications in various organisms.

关键词： system attractive rational

Genetic diversity and phylogenetic analysis of Chinese Han and Li ethnic populations from Hainan Island by 30 autosomal insertion/deletion polymorphisms

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Forensic Sciences Research 2022年第2期7卷 189-195页

作者： Jing Liu Ziwei Ye Zheng wang Xing Zou Guanglin He mengge wang Shouyu wang Yiping Hou Institute of Forensic Medicine West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengduChina

With the characteristics of low mutation rate,length variation and short amplicon size,insertion/deletion polymorphisms(InDels)have the advantages of both short tandem repeats(STRs)and single nucleotide polymorphisms(SNPs).Herein,people of two ethnicities from Hainan Island were genotyped for the first time using the Investigator DIPplex kit.We investigated the forensic parameters of the 30 InDels and the phylogenetic relationships among different populations.The accumulated powers of discrimination and powers of exclusion were 0.9999999999646 and 0.9897 in the Hainan Han population and 0.9999999999292 and 0.9861 in the Hainan Li population,respectively.Additionally,population comparisons among geographically,ethnically and linguistically diverse populations via cluster heatmap,multidimensional scaling,principal component analysis,phylogenetic tree and STRUCTURE analyses demonstrated that the Hainan Han population had genetic similarities to the other Han,She and Tujia populations,while the Hainan Li population had close genetic relationships to the Zhuang and Miao groups;both populations had a high degree of genetic differentiation from most Turkic-speaking populations.Aforementioned results suggested that the 30 autosomal InDels are highly polymorphic and informative,which are suitable for human identification and population genetics.

关键词： Forensic sciences forensic genetics InDels Investigator DIPplex kit Hainan Han Hainan Li

Functional Lactiplantibacillus plantarum P10 screened from Chinese traditional pickle showing application potential in steamed bread for in-situ bio-preservation and GABA-enrichment

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Food Science and Human Wellness 2024年

作者： mengge Ma Yajuan Zhang Dongchun Zhang Yi Chen Zhixin wang Yingmin Jia Yawei Ning Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Technology and Business University College of Food and Biology Hebei University of Science and Technology

Mold spoilage and nutrition enrichment are major concerns of Chinese steamed bread， and using functional lactic acid bacteria as starter to play the role of in-situ preservation and nutritional fortification was a promising alternative. Hence， Lactiplantibacillus plantarum P10 capable of inhibiting spoilage microorganisms and producing GABA (380 mg/L) was screened from Chinese pickle. Metabolomics analysis showed that P10 produced key antimicrobial metabolites mainly including ten organic acids， aldehyde， alkaloid. Besides， P10 might exhibit health beneficial effects such as degrading cholesterol， lowering blood glucose and reducing uric acid due to the production of deoxycholic acid and Ile-Pro-Ile， and the degradation of purine. Finally， the promising application potential of P10 was evidenced in steamed bread with extended shelf life， excellent anti-aging effect， and 24.5-fold enrichment of GABA. Conclusively， this investigation could provide scientific basis for the application of P10 as a functional starter in steamed bread.

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Population genetics and forensic efficiency of 30 InDel markers in four Chinese ethnic groups residing in Sichuan

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Forensic Sciences Research 2022年第3期7卷 498-502页

作者： Fei wang Guanglin He Zheng wang mengge wang Jing Liu Xing Zou Shouyu wang Mengyuan Song Ziwei Ye Mingkun Xie Yiping Hou Institute of Forensic Medicine West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengduChina

Sichuan Province is located at the transitional junction regions of the Qinghai-Tibet Plateau and the low-altitude plains.It also serves as the corridor of Sino Tibetan-speaking population migration and expansion since neolithic expansion of Proto-Tibeto-Burman populations from Middle/Upper Yellow River during Majiayao period(3300-2000 BC).However,the population structure and the corresponding genetic diversity of forensic-related markers in this region remain unclear.Thus,we genotyped 30 insertion-deletion(InDel)markers in 444 samples from four ethnic groups(Han,Tibetan,Hui and Yi)from Sichuan Province using the Investigator■ DIPplex kit to explore the characteristics of population genetics and forensic genetic focuses.All the loci were found to be in Hardy-Weinberg Equlibrium(HWE)after applying a Bonferroni correction and no pairwise loci showed prominent linkage disequilibrium.The combined matching probability(CMP)and the combined power of discrimination(CPD)are larger than 1.8089×10^(11)and 0.9999999995,respectively.Principal component analysis,multi-dimen-sional scaling plots and Neighbour-Joining tree among 65 worldwide populations indicated that Sichuan Hui and Han are genetically close to Hmong-Mien and Tai-Kadai-speaking popula-tions,and Sichuan Tibetan and Yi bear a strong genetic affinity with Tibeto-Burman-speaking populations.The model-based genetic structure further supports the genetic affinity between the studied populations and linguistically close populations.

关键词： Forensic sciences InDel population genetics Sichuan Han Sichuan Yi Sichuan Tibetan Sichuan Hui

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双机器人协作轴孔装配力柔顺控制技术研究

机械工程学报 2024年第3期60卷 20-33页

作者：王战玺张益铭张邦海罗子彦史梦格西北工业大学机电学院西安710072 南京电子技术研究所南京210012

轴孔精密装配是加工制造业中常见的装配形式,其完成过程同时存在位置约束和力约束关系,因此采用工业机器人实现轴孔自动装配一直是具有挑战性的热点问题。首先分析了轴孔装配过程插孔阶段的典型的单点接触和双点接触受力模型,并针对不... 详细信息

轴孔精密装配是加工制造业中常见的装配形式,其完成过程同时存在位置约束和力约束关系,因此采用工业机器人实现轴孔自动装配一直是具有挑战性的热点问题。首先分析了轴孔装配过程插孔阶段的典型的单点接触和双点接触受力模型,并针对不同的接触状态提出了机器人整体力调控策略;其次在基于位置的阻抗模型基础上,建立对环境位置和刚度具有自适应效果的力柔顺控制模型,通过仿真验证了控制模型可以消除力跟踪过程的系统稳态误差;然后建立了主从式双机器人协作轴孔装配模型,并进行轴孔装配仿真实验,在不同的机器人位置和姿态误差下验证双机器人协作装配的力位控制效果;最后搭建主从式双机器人装配实验平台,验证自适应力柔顺控制模型和主从式协作模型的可行性。

关键词：轴孔装配力柔顺控制阻抗控制主从式协作

CT/MRI图像融合技术与CT引导三叉神经半月节射频热凝术的疗效对比

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安徽医科大学学报 2024年第3期59卷 538-541,546页

作者：沈静王梦格宋阳任振华安徽医科大学第一附属医院疼痛科合肥230022 安徽医科大学人体解剖学教研室合肥230032

目的对比CT/MRI图像融合技术和传统CT引导经皮三叉神经半月节射频热凝术的疗效。方法收集88例原发性三叉神经痛患者医疗信息,按不同影像引导方式随机平均分为对照组(CT引导三叉神经半月节射频热凝术)和融合组(CT/MRI图像融合技术辅助三... 详细信息

目的对比CT/MRI图像融合技术和传统CT引导经皮三叉神经半月节射频热凝术的疗效。方法收集88例原发性三叉神经痛患者医疗信息,按不同影像引导方式随机平均分为对照组(CT引导三叉神经半月节射频热凝术)和融合组(CT/MRI图像融合技术辅助三叉神经半月节射频热凝术);对穿刺操作时间、术中不适发生率以及术前、术中和术后的视觉模拟评分法(VAS)评分、巴罗神经学研究所(BNI)疼痛评分及术后并发症发生率进行比较。结果融合组穿刺操作耗时短于对照组(P<0.05);融合组术中和术后的VAS及BNI评分、术中不适、术后并发症发生率均低于对照组(P<0.05)。结论在提高疗效、减少术中不适、术后并发症方面,CT/MRI图像融合技术较CT引导更具优越性。

关键词： CT/MRI图像融合三叉神经痛射频热凝术半月神经节 CT引导术后并发症

基于SNA的煤矿工人安全信息认知影响因素研究

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煤矿安全 2024年第3期55卷 244-248页

作者：李磊折亚亚马梦格王帆西安科技大学安全科学与工程学院陕西西安710054 西部矿井开采及灾害防治教育部重点实验室陕西西安710054

大多数煤矿事故是由人的不安全行为导致的,而人的不安全行为是煤矿工人安全信息认知水平力低下造成的。为预防和控制煤矿工人在安全信息认知失效下引发的煤矿安全事故,通过分析我国近年典型的煤矿事故案例,确定影响煤矿工人安全信息认知... 详细信息

大多数煤矿事故是由人的不安全行为导致的,而人的不安全行为是煤矿工人安全信息认知水平力低下造成的。为预防和控制煤矿工人在安全信息认知失效下引发的煤矿安全事故,通过分析我国近年典型的煤矿事故案例,确定影响煤矿工人安全信息认知的3个一级指标和15个二级指标,并利用社会网络分析法构建煤矿工人安全信息认知网络;通过对安全信息认知因素进行整体网络密度、个体网络密度、节点的度数中心度、节点的中间中心度、关键影响因素和重要影响路径,得出辨识理解能力、安全知识等5个关键影响因子,并识别出关键影响路径。

关键词：煤矿工人安全信息认知社会网络分析影响因素关键影响路径

耕作和秸秆还田对球囊霉素和土壤生态化学计量特征的影响

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山东农业科学 2024年第2期56卷 118-123页

作者：李扬张梦歌王震石兆勇河南科技大学农学院/河南省乡村人居环境工程中心/洛阳市共生微生物与绿色发展重点实验室河南洛阳471000

为探究耕作与秸秆还田对土壤球囊霉素相关蛋白含量、生态化学计量特征以及两者之间关系的影响,本研究选择长期进行作物生产的农场土壤,采集免耕、耕作和耕作秸秆还田三种处理下的0~20 cm土层土壤样品进行分析。结果表明,免耕土壤总球囊... 详细信息

为探究耕作与秸秆还田对土壤球囊霉素相关蛋白含量、生态化学计量特征以及两者之间关系的影响,本研究选择长期进行作物生产的农场土壤,采集免耕、耕作和耕作秸秆还田三种处理下的0~20 cm土层土壤样品进行分析。结果表明,免耕土壤总球囊霉素含量为4.1 mg/g,较耕作土壤显著提高5.1%,耕作秸秆还田土壤较耕作土壤提高3.6%,免耕和耕作秸秆还田土壤易提取球囊霉素含量分别较耕作土壤提高4.8%和6.1%。免耕和耕作秸秆还田土壤有机碳含量分别为17.58、17.89 mg/g,较耕作土壤显著提高14.1%、16.1%;免耕土壤全氮含量为1.49 mg/g,较耕作和耕作秸秆还田土壤分别显著提高26.3%、17.3%。耕作秸秆还田土壤碳氮比达到14.47,显著高出免耕土壤33.2%。方差分解分析及线性相关分析表明,土壤有机碳、全氮含量影响球囊霉素的分泌和释放,免耕和耕作秸秆还田有利于提高土壤有机碳、全氮含量,从而促进球囊霉素的分泌,而长期耕作对丛枝菌根真菌分泌土壤球囊霉素具有限制作用。

关键词：耕作秸秆还田球囊霉素土壤有机碳全氮全磷