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Electron transfer in protein modifications:from detection to imaging

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Science China Chemistry 2023年第2期66卷 388-405页

作者： Lei Zhang mengge chu Cailing Ji Wenjie Wang Jie Tan Quan Yuan Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha410082China

Signal pathways participate in vital biological processes and regulate complex life activities through protein modifications.Protein modifications in signal pathways are accompanied by electron transfer.The study of electronic behavior helps to explore the physical and chemical processes in signal pathways,receiving extensive attention.There are some excellent reviews that have summarized methods for signal pathway detection,while few discussions are from an electron transfer perspective.This review describes the relationship between signal pathways and electron transfer in protein modification.Subsequently,we summarize the electron transfer-based detection methods,such as electrochemical,photoelectrochemical and electrochemiluminescence methods.Additionally,the applications of signal pathway detection in mechanism study and imaging are also reviewed.Finally,a comprehensive discussion of the summary and outlooks in this field is presented,aiming to provide valuable guidance for the molecular mechanism of life processes and the development of new analytical techniques.

关键词： protein modification electron transfer signal pathway mechanism imaging

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我国农业全产业链标准化转型路径研究

中国工程科学 2024年第2期26卷 142-149页

作者：燕艳华云振宇初侨奚经龙席兴军任梦格张晓芳王宇彤中国标准化研究院农业食品标准化研究所北京100191 浙江省标准化研究院(浙江省标准化智库) 杭州310063 中国农业大学人文与发展学院北京100083

推进农业全产业链标准化,既是保证我国农业产业安全稳定的内在要求,也是推进农业产业转型升级的必要选择。当前,我国农业全产业链标准化转型主要面临标准供给结构仍需持续优化,产前、产中、产后标准间协同性亟待增强,标准化的联动障碍... 详细信息

推进农业全产业链标准化,既是保证我国农业产业安全稳定的内在要求,也是推进农业产业转型升级的必要选择。当前,我国农业全产业链标准化转型主要面临标准供给结构仍需持续优化,产前、产中、产后标准间协同性亟待增强,标准化的联动障碍依然突出等问题与挑战。本文从农业全产业链标准化转型对供应链稳定与安全、各环节要素流动与畅通、生产经营方式的变革与创新、品牌内核力与竞争力提升、绿色化发展与低碳转型等五方面出发,系统剖析了其在标准结构、标准协同、标准联动等方面面临的挑战,提出了农业全产业链标准化转型应以农业产业综合性和复杂性问题为导向,以产前、产中、产后标准协同为核心,以高质量标准有效供给为前提,以各方利益主体共同参与为保障四方面的总体方略。研究建议,着力提升高质量标准的有效供给、推进全产业链标准的协同化治理、创新地方标准“互联互通”机制、强化标准实施机制创新和主体参与。

关键词：农业全产业链标准化高质量标准标准协同

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Associative learning of a three-terminal memristor network for digits recognition

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Science China(Information Sciences) 2023年第2期66卷 209-218页

作者： Yiming REN Bobo TIAN mengge YAN Guangdi FENG Bin GAO Fangyu YUE Hui PENG Xiaodong TANG Qiuxiang ZHU Junhao chu chungang DUAN Key Laboratory of Polar Materials and Devices (MOE) Ministry of Education Department of ElectronicsEast China Normal University Zhejiang Lab Institute of Microelectronics Beijing Innovation Center for Future Chips (ICFC) Tsinghua University Collaborative Innovation Center of Extreme Optics Shanxi University

Imitating the associative intelligence of the biological brain is attractive but is poorly achieved in hardware because the complex tunable connection in neural networks is difficult to reproduce. We develop a circuit composed of a three-terminal memristor network to reproduce the biological conditioning process artificially. The synaptic weight between co-firing neurons is strengthened simultaneously by generating a feedback signal from the integrate-and-fire neuron to the gate of the synaptic memristor. The network allows the multi-associative capacity of recalling more than one digit in one circuit. Both single and multi-associative learning for recalling digital images are achieved. Furthermore, all 10 digital images from “0” to “9” are successfully recalled in an associative network with such paralleling circuits. Assisted by this associative layer, a typical classification network effectively improves the recognition rate for fragmentary digital images.Our work sheds light on brain-inspired artificial associative memory and provides a strategy for applications,such as object recognition with partial features and similar scenes.

关键词： artificial intelligence associative learning memristor classification network

果糖二磷酸醛缩酶C在结肠癌组织和细胞中的表达及其对生物学行为的影响

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实用医学杂志 2023年第15期39卷 1893-1900页

作者：程多褚菲菲张楠王晶晶陈梦阁岳文莉郜辉梁芳郑州大学附属郑州中心医院肿瘤康复科病区郑州450000 郑州大学附属郑州中心医院消化内科郑州450000

目的观察果糖二磷酸醛缩酶C(fructose-bisphosphate aldolase C,ALDOC)在结肠癌组织和细胞中的表达及其对生物学行为的影响。方法利用GEPIA数据库分析结肠癌组织ALDOC的表达水平。以患者癌旁组织或正常结肠上皮细胞FHC为对照,RT-qPCR... 详细信息

目的观察果糖二磷酸醛缩酶C(fructose-bisphosphate aldolase C,ALDOC)在结肠癌组织和细胞中的表达及其对生物学行为的影响。方法利用GEPIA数据库分析结肠癌组织ALDOC的表达水平。以患者癌旁组织或正常结肠上皮细胞FHC为对照,RT-qPCR及免疫组化检测结肠癌组织和癌细胞SW620、SW480、HT-29、HCT-116中ALDOC mRNA和蛋白的表达,并分析ALDOC mRNA表达与患者临床病理因素的关系。将结肠癌细胞SW620分为对照组、NC inhibitor组和ALDOC inhibitor组;通过Lipofectamine2000将ALDOC inhibitor或阴性对照转染各组SW620细胞;MTT法及癌细胞单克隆形成检测细胞增殖能力;划痕实验检测细胞迁移能力;Transwell小室实验检测细胞侵袭能力;RT-qPCR检测细胞ALDOC mRNA的表达;Western blot检测细胞ALDOC、金属基质蛋白酶-2/9(MMP-2/9)的表达。结果 GEPIA数据库显示结肠癌组织ALDOC的表达水平高于正常组织(P <0.05);与癌旁组织或FHC相比,结肠癌组织和细胞SW620、SW480、HT-29、HCT-116中ALDOC mRNA和蛋白上调,且ALDOC mRNA水平与患者肿瘤分化程度、TNM分期、淋巴结转移有明显的相关性(P <0.05);下调ALDOC mRNA能够下调MMP-2、MMP-9蛋白水平,抑制细胞生长,减少划痕愈合和侵袭细胞数(P <0.05)。结论 ALDOC水平在结肠癌组织和细胞中表达上调,且与结肠癌患者的不良预后紧密相关。ALDOC通过调节MMP-2/9调控结肠癌细胞的侵袭和迁移能力。

关键词：果糖二磷酸醛缩酶C 结肠癌侵袭迁移增殖

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