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Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA

World Journal of Pediatrics 2019年第5期15卷 499-505页

作者： Duo Zhou meng-ying zhu Yi-Long Wang Xiao-Qiang Hao Dong-Ming Zhou Rong-Xian Liu Chu-Di Zhang Chu-Fan Qu Zheng-Yan Zhao Zhejiang University School of Medicine HangzhouChina Children's Hospital Zhejiang University School of MedicineHangzhou 310052China Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310052China

Background Mumps is a common type of respiratory infectious disease caused by mumps virus(MuV),and can be effectively prevented by *** this study,a reverse genetic system of MuV that can facilitate the rational design of safer,more efficient mumps vaccine candidates is *** MuV-S79 cDNA clone was assembled into a full-length plasmid by means of the GeneArtTM High-Order Genetic Assembly System,and was rescued via reverse genetic ***-PCR,sequencing,and immunofluorescence assays were used for rMuV-S79 *** replication kinetics and in vivo experimental models were used to evaluate the replication,safety,and immunogenicity of *** A full-length cDNA clone of MuV-S79 in the assembly process was generated by a novel plasmid assemble strategy,and a robust reverse genetic system of MuV-S79 was successfully *** established rMuV-S79 strain could reach a high virus titer in *** average viral titer of rMuV-S79 in the lung tissues was 2.68±0.14 log10PFU/g lung tissue,and rMuV-S79 group did not induce inflammation in the lung tissues in cotton *** antibody titers induced by rMuV-S79 were high,long-lasting and could provide complete protection against MuV wild strain *** We have established a robust reverse genetic system of MuV-S79 which can facilitate the optimization of mumps ***-S79 rescued could reach a high virus titer and the safety was proven in *** could also provide complete protection against MuV wild strain challenge.

关键词： Mumps virus Reverse genetics Plasmid Safety Immunogenicity

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Attenuated MuV-S79 as vector stably expressing foreign gene

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World Journal of Pediatrics 2019年第5期15卷 511-515页

作者： Duo Zhou meng-ying zhu Yi-Long Wang Xiao-Qiang Hao Dong-Ming Zhou Rong-Xian Liu Chu-Di Zhang Chu-Fan Qu Zheng-Yan Zhao Zhejiang University School of Medicine HangzhouZhejiangChina Children's Hospital Zhejiang University School of MedicineHangzhou 310052China Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310052China

Background To describe mumps virus(MuV)used as a vector to express enhanced green fluorescent protein(EGFP)or red fluorescent protein(RFP)*** Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses(rMuVs).rMuVs were recovered based on our reverse genetic system of *** properties of rMuVs were determined by growth curve,plaque assay,fluorescent microscopy and determination of fluorescent *** Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79,expressed heterologous genes in high levels,and were genetically stable in at least 15 *** rMuV-S79 is a promising platform to accommodate foreign genes like marker genes,other antigens and immunomodulators for addressing various diseases.

关键词： Mumps virus Reverse genetic system Foreign genes

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FinBrain: when finance meets AI 2.0

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Frontiers of Information Technology & Electronic Engineering 2019年第7期20卷 914-925页

作者： Xiao-lin ZHENG meng-ying zhu Qi-bing LI Chao-chao CHEN Yan-chao TAN Department of Computer Science Zhejiang UniversityHangzhou310027China AI Department Ant Financial Services GroupHangzhou310027China

Artificial intelligence(AI)is the core technology of technological revolution and industrial *** one of the new intelligent needs in the AI 2.0 era,financial intelligence has elicited much attention from the academia and *** our current dynamic capital market,financial intelligence demonstrates a fast and accurate machine learning capability to handle complex data and has gradually acquired the potential to become a'financial brain.'In this paper,we survey existing studies on financial ***,we describe the concept of financial intelligence and elaborate on its position in the financial technology ***,we introduce the development of financial intelligence and review state-of-the-art techniques in wealth management,risk management,financial security,financial consulting,and ***,we propose a research framework called FinBrain and summarize four open issues,namely,explainable financial agents and causality,perception and prediction under uncertainty,risk-sensitive and robust decision-making,and multi-agent game and mechanism *** believe that these research directions can lay the foundation for the development of AI 2.0 in the finance field.

关键词： Artificial intelligence Financial intelligence

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Proteomic profiling of cerebrospinal fluid in pediatric myelin oligodendrocyte glycoprotein antibody-associated disease

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World Journal of Pediatrics 2024年第3期20卷 259-271页

作者： Yi-Long Wang meng-ying zhu Zhe-Feng Yuan Xiao-Yan Ren Xiao-Tong Guo Yi Hua Lu Xu Cong-ying Zhao Li-Hua Jiang Xin Zhang Guo-Xia Sheng Pei-Fang Jiang Zheng-Yan Zhao Feng Gao Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310052China Children's Hospital Zhejiang University School of MedicineHangzhou 310052China Children's Hospital National Clinical Research Center for Child HealthZhejiang University School of MedicineHangzhou 310052China

Background Myelin oligodendrocyte glycoprotein(MOG)antibody-associated disease(MOGAD)is an autoimmune demy-elinating disorder of the central nervous *** Extracted proteins from 34 cerebrospinal fluid(CSF)samples[patients with MOGAD(MOG group,n=12);healthy controls(HC group,n=12);patients with MOG seronegative and metagenomics next-generation sequencing-negative inflammatory neurological diseases(IND group,n=10)]were processed and subjected to label-free quantitative *** partial least squares-discriminant analysis(PLS-DA)and orthogonal PLS-DA(O-PLS-DA)models were also performed based on proteomics *** analysis of differentially expressed proteins(DEPs)was performed using Gene Ontology,InterPro,and Kyoto Encyclopedia Genes and *** enzyme-linked immunosorbent assay was used to determine the complement levels in serum from patients with *** Four hundred and twenty-nine DEPs(149 upregulated and 280 downregulated proteins)were identified in the MOG group compared to the HC group according to the P value and fold change(FC).Using the O-PLS-DA model,872 differentially abundant proteins were identified with variable importance projection(VIP)scores>*** proteins(gamma-glutamyl hydrolase,cathepsin F,interalpha-trypsin inhibitor heavy chain 5,latent transforming growth factor beta-binding protein 4 and leukocyte-associated immunoglobulin-like receptor 1)overlapping between the top 30 DEPs with top-ranked P value and FC and top 30 proteins in PLS-DA VIP lists were *** analysis revealed that the dysregulated proteins in the MOG group were primarily involved in complement and coagulation cascades,cell adhesion,axon guidance,and glycosphingolipid biosynthesis compared to the HC *** The proteomic alterations in CSF samples from children with MOGAD identified in the current study might provide opportunities for developing novel biomarker candidates.

关键词： Acute disseminated encephalomyelitis Cerebrospinal fluid Complement cascades Myelin oligodendrocyte glycoprotein-associated disease Proteomics

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古化石与陨石的元素对比分析

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光谱学与光谱分析 2017年第1期37卷 247-249页

作者：祁丽杰王莹孟庆竹赵震沈阳师范大学化学化工学院辽宁沈阳110034

古化石是古代的动植物经过漫长地质时期和地质作用形成的化石,它是地球历史的鉴证,记载着地球生态环境演变的信息;长时间遨游于太阳系中的陨石,经过宇宙间的核反应和大气的磨蚀,形成了独特的纹理与造型,记载着外太空形成演化的原始信息... 详细信息

古化石是古代的动植物经过漫长地质时期和地质作用形成的化石,它是地球历史的鉴证,记载着地球生态环境演变的信息;长时间遨游于太阳系中的陨石,经过宇宙间的核反应和大气的磨蚀,形成了独特的纹理与造型,记载着外太空形成演化的原始信息。古化石成分的分析是研究地球生物起源和进化的科学依据;而陨石是大自然馈赠的来自外太空的岩石样品,极为珍贵和稀少,对陨石成分的分析可以为我国外星球探测和天体化学的发展提供更好的基础。对样品的溶解方法进行选择和优化,最终以硝酸、氢氟酸、高氯酸为混合试剂,采用微波消解法溶解古化石样品;以王水、硝酸、氢氟酸、高氯酸为混合试剂,采用微波消解与湿法消解相结合溶解陨石样品,应用电感耦合等离子体原子发射光谱法(ICP-AES)测定了3种古化石样品、3种陨石样品中多种元素的含量,并将古化石与陨石中相同元素的含量进行对比。分析结果表明,古化石和陨石中都含有Pb,Hg,Ge,Fe,Ni,Cu,Co和Mn等重金属元素,以及部分稀土元素。该方法加标回收率在98.2%~106%之间,相对标准偏差均小于2.12%。方法的灵敏度高,准确度和精密度好。

关键词：陨石古化石 ICP-AES

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Near-perfect replication on amorphous alloys through active force modulation based on machine learning/neural network parameter prediction

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 181-191页

作者： Senkuan meng Zheng Wang Ruisong zhu Ruijie Liu Jiang Ma Lina Hu Weihua Wang Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education) School of Materials Science and Engineering Shandong University College of Mechatronics and Control Engineering Shenzhen University Songshan Lake Materials Laboratory Institute of Physics Chinese Academy of Sciences

As a microforming technique, micro/nano-structural replication possesses advantages of high precision and efficiency. With the remarkable superplasticity in the supercooled liquid region, amorphous alloys or metallic glasses(MGs) are regarded as ideal materials for miniature fabrication. However, due to the intrinsic metastable nature of supercooled liquids, the design of imprinting processes for MGs poses a challenge. In the past, process parameters have largely relied on trial-and-error *** this work, a low-frequency active force modulation system is employed to apply a stable, precise, and real-time feedback stress field for imprinting of MG samples. Low-frequency vibrations can facilitate the filling of microstructures on the template surface by reducing the effective viscosity of the supercooled liquid. With the dataset composed of orthogonal experiments, a machine learning strategy based on back-propagation(BP) neural networks was utilized to construct a 3D parameter space for temperature, stress, and time, and to predict the corresponding filling ratio. Furthermore, the optimal combination of imprinting process parameters was identified, and its filling ratio was experimentally validated to reach as high as 0.94. The near-perfect replication of microstructures confirms the superiority of the active force modulation system and the data-driven strategy of machine learning-assisted parameter design. At the same time, this one-step microforming process provides a new approach to addressing the accuracy-cost trade-off dilemma in precision manufacturing.

关键词： metallic glass amorphous alloy microforming imprinting machine learning

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Dip2a regulates stress susceptibility in the basolateral amygdala

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Neural Regeneration Research 2025年第6期20卷 1735-1748页

作者： Jing Li Zixuan He Weitai Chai meng Tian Huali Yu Xiaoxiao He Xiaojuan zhu Key Laboratory of Molecular Epigenetics Ministry of Education Institute of Genetics and CytologyNortheast Normal UniversityChangchunJilin ProvinceChina

Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. Our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.

关键词： 5-hydroxytryptamine acute restraint stress basolateral amygdala CaMKII neurons DIP2A metabolomics neurotransmitters principal component analysis stress susceptibility tryptophan

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以产业转型规划为先促进城镇化新市建设

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科技创新与生产力 2011年第7期 38-40页

作者：孟竹英晋中市城乡规划设计研究院山西晋中030600

在分析晋中市城镇化发展现状的基础上,指出面对区域"边缘化"带来的挑战,晋中市要想走出资源依赖困境,必须结合晋中市的特点和资源条件,坚定不移地以构建"一核一圈三群"为战略目标,主动融入、主动转型、集约发展,积极推进产业转型,从而... 详细信息

在分析晋中市城镇化发展现状的基础上,指出面对区域"边缘化"带来的挑战,晋中市要想走出资源依赖困境,必须结合晋中市的特点和资源条件,坚定不移地以构建"一核一圈三群"为战略目标,主动融入、主动转型、集约发展,积极推进产业转型,从而促进晋中城镇化建设的可持续发展。

关键词：区域边缘化资源依赖一核一圈三群产业转型晋中

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EZH2-dependent myelination following sciatic nerve injury

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Neural Regeneration Research 2025年第8期20卷 2382-2394页

作者： Hui zhu Li Mu Xi Xu Tianyi Huang ying Wang Siyuan Xu Yiting Wang Wencong Wang Zhiping Wang Hongkui Wang Chengbin Xue Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityKey Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Department of Neurology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Critical Care Medicine Nantong Fourth People’s HospitalNantongJiangsu ProvinceChina

Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve ***,the gene regulatory network of regenerated myelin differs from that of native *** of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in *** further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were *** results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain *** highlights the crucial role of Ezh2 in initiating Schwann cell ***,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type *** suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and *** conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve *** EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.

关键词： demyelination EZH2 myelination peripheral nerve injury PRC2 remyelination Schwann cells sciatic nerve crush sciatic nerve transection

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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： meng Cong Jing-Jing Hu Yan Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie zhu Xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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