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Genetic mutation of Tas2r104/Tas2r105/Tas2r114 cluster leads to a loss of taste perception to denatonium benzoate and cucurbitacin B

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Animal Models and Experimental Medicine 2024年第3期7卷 324-336页

作者： Bowen Niu Lingling Liu Qian Gao meng-min zhu Lixiang Chen Xiu-Hua Peng Boying Qin Xiaohui Zhou Feng Li Department of Laboratory Animal Science Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Department of Biology College of Life SciencesShanghai Normal UniversityShanghaiPeople's Republic of China

Background:Bitter taste receptors(Tas2rs)are generally considered to sense various bitter compounds to escape the intake of toxic *** taste receptors have been found to widely express in extraoral tissues and have important physiological functions outside the gustatory system in ***:To investigate the physiological functions of the bitter taste receptor cluster Tas2r106/Tas2r104/Tas2r105/Tas2r114 in lingual and extraoral tissues,multiple Tas2rs mutant mice and Gnat3 were produced using CRISPR/Cas9 gene-editing technique.A mixture containing Cas9 and sgRNA mRNAs for Tas2rs and Gnat3 gene was microinjected into the cytoplasm of the ***,T7EN1 assays and sequencing were used to screen genetic mutation at the target sites in founder *** real-time polymerase chain reaction(qRT-PCR)and immunostaining were used to study the expression level of taste signaling cascade and bitter taste receptor in taste *** to taste substance was also studied using twobottle preference ***:We successfully produced several Tas2rs and Gnat3 mutant mice using the CRISPR/Cas9 *** results showed that the expression of GNAT3 and PLCB2 was not altered in Tas2rs mutant *** qRT-PCR results revealed the changed expression profile of m Tas2rs gene in taste buds of these mutant *** two-bottle preference tests,these mutant mice eliminate responses to cycloheximide due to genetic mutation of *** addition,these mutant mice showed a loss of taste perception to quinine dihydrochloride,denatonium benzoate,and cucurbitacin B(CuB).Gnat3-mediated taste receptor and its signal pathway contribute to CuB ***:These findings implied that these mutant mice would be a valuable means to understand the biological functions of TAS2Rs in extraoral tissues and investigate bitter compound-induced responses mediated by these TAS2Rs in many extraoral tissues.

关键词： bitter taste receptor CRISPR/Cas9 genetic mutation two-bottle preference test type 2 taste receptors(Tas2rs)

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Ablation of NTPDase2+ cells inhibits the formation of filiform papillae in tongue tip

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Animal Models and Experimental Medicine 2018年第2期1卷 143-151页

作者： Feng Li Bo-Wen Niu meng-min zhu Department of Laboratory Animal Science Shanghai Public Health Clinical CenterFudan University

Background: Lingual epithelia in the tongue tip are among the most rapidly regenerating tissues, but the mechanism of cell genesis in this tissue is still unknown. Previous study has suggested the existence of multiple stem cell pools in lingual epithelia and papillae. Like K14+ and Sox2+ cells, NTPDase2+ cells have characteristics of stem ***: We employed a system using doxycycline to conditionally ablate NTPDase2+ cells in lingual epithelia and papillae by regulated expression of the diphtheria toxin A(DTA) gene. Transgenic lines, which expressed the rtTA gene in NTPDase2+ cells, were produced by pronuclear injection of zygotes from C57 BL/6 mice using the BAC clone RP23-47 P18. The NTPDase2-rtTA transgenic mice were crossed with the TetO-DTA transgenic animals. The double transgenic mice were treated with doxycycline. Doxycycline(Dox) was diluted in 5% sucrose in water to a final concentration of 0.3-0.5 mg/mL and supplied as drinking ***: After 15 days of Dox induction, the expression of NTPDase2, Sox2 and K14 was ablated from lingual epithelia. DTA expression in NTPDase2+cells did not inhibit the turnover of GNAT3+ or PLCb2+ cells in taste buds,nor the expression of S100 b beneath lingual epithelia and papillae. After35 days ablation of NTPDase2+ cells, the basic structure of lingual epithelia and papillae remained intact. However, the ratio of cell to total tissue area was decreased in lingual epithelia and circumvallate(CV) papillae. DTA expression also inhibited the regeneration of filiform papillae on the dorsal surface of the tongue ***: These studies provide important insights into the understanding of dynamic equilibrium among the multiple stem cell populations present in the lingual epithelia and papillae.

关键词： cell ablation lingual epithelia NTPDase2 papillae tissue regeneration

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A multiscale adaptive framework based on convolutional neural network: Application to fluid catalytic cracking product yield prediction

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Petroleum Science 2024年第4期21卷 2849-2869页

作者： Nan Liu Chun-meng zhu meng-Xuan Zhang Xing-Ying Lan State Key Laboratory of Heavy Oil Processing China University of Petroleum (Beijing) College of Artificial Intelligence China University of Petroleum (Beijing)

Since chemical processes are highly non-linear and multiscale, it is vital to deeply mine the multiscale coupling relationships embedded in the massive process data for the prediction and anomaly tracing of crucial process parameters and production indicators. While the integrated method of adaptive signal decomposition combined with time series models could effectively predict process variables, it does have limitations in capturing the high-frequency detail of the operation state when applied to complex chemical processes. In light of this, a novel Multiscale Multi-radius Multi-step Convolutional Neural Network(Msrt Net) is proposed for mining spatiotemporal multiscale information. First, the industrial data from the Fluid Catalytic Cracking(FCC) process decomposition using Complete Ensemble Empirical Mode Decomposition with Adaptive Noise(CEEMDAN) extract the multi-energy scale information of the feature subset. Then, convolution kernels with varying stride and padding structures are established to decouple the long-period operation process information encapsulated within the multi-energy scale data. Finally, a reconciliation network is trained to reconstruct the multiscale prediction results and obtain the final output. Msrt Net is initially assessed for its capability to untangle the spatiotemporal multiscale relationships among variables in the Tennessee Eastman Process(TEP). Subsequently, the performance of Msrt Net is evaluated in predicting product yield for a 2.80 × 10～6 t/a FCC unit, taking diesel and gasoline yield as examples. In conclusion, Msrt Net can decouple and effectively extract spatiotemporal multiscale information from chemical process data and achieve a approximately reduction of 30% in prediction error compared to other time-series models. Furthermore, its robustness and transferability underscore its promising potential for broader applications.

关键词： Fluid catalytic cracking Product yield Data-driven modeling Multiscale prediction Data decomposition Convolution neural network

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Compound Product of Hypermatrices

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Journal of Systems Science & Complexity 2024年第1期37卷 169-183页

作者： CHENG Daizhan meng min ZHANG Xiao JI Zhengping Key Laboratory of Systems and Control Academy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190China Department of Control Science and Engineering School of Electronics and Information EngineeringTongji UniversityShanghai 201210China National Center for Mathematics and Interdisciplinary Sciences Chinese Academy of SciencesBeijing 100190China School of Mathematical Sciences University of Chinese Academy of SciencesBeijing 100149China.

The semi-tensor product(STP)of matrices is generalized to multidimensional arrays,called the compound product of *** product is first defined for three-dimensional hypermatrices with compatible orders and then extended to general *** different types of hyperdeterminants are introduced and certain properties are *** Lie groups and Lie algebras corresponding to the hypermatrix products are ***,these results are applied to dynamical systems.

关键词： Compound semi-tensor product hyperdeterminants hypereigenvalues and hypereigenvectors hypergeneral linear groups hypermatrices.

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A survey of decision making in adversarial games

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Science China(Information Sciences) 2024年第4期67卷 85-112页

作者： Xiuxian LI min meng Yiguang HONG Jie CHEN College of Electronics and Information Engineering Tongji University Shanghai Research Institute for Intelligent Autonomous Systems

In many practical applications, such as poker, chess, drug interdiction, cybersecurity, and national defense, players often have adversarial stances, i.e., the selfish actions of each player inevitably or intentionally inflict loss or wreak havoc on other players. Therefore, adversarial games are important in realworld applications. However, only special adversarial games, such as Bayesian games, are reviewed in the literature. In this respect, this study aims to provide a systematic survey of three main game models widely employed in adversarial games, i.e., zero-sum normal-form and extensive-form games, Stackelberg(security) games, and zero-sum differential games, from an array of perspectives, including basic knowledge of game models,(approximate) equilibrium concepts, problem classifications, research frontiers,(approximate) optimal strategy-seeking techniques, prevailing algorithms, and practical applications. Finally, promising future research directions are also discussed for relevant adversarial games.

关键词： adversarial games zero-sum games Stackelberg games differential games Nash equilibrium correlated equilibrium regret

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Constraining the Earth-mass Primordial Black Hole Mergers Model of the Non-repeating FRBs Using the First CHIME/FRB Catalog

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Research in Astronomy and Astrophysics 2024年第9期24卷 76-82页

作者： min meng Qiu-Ju Huang Can-min Deng Guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China

In this paper,we upgrade the constraints for the Earth-mass primordial black hole mergers model based on the first Canadian Hydrogen Intensity Mapping Experiment(CHIME)/fast radio burst(FRB)*** the null hypothesis that the observed non-repeating FRBs originate from Earth-mass primordial black hole mergers,we find that how the charges were distributed in the primordial black hole population is well described by a double powerlaw function with typical charge value of law function with typical charge value of q_(c)/10^(-5)=1.60_(-0.28)^(+0.28),where the power-law index α_(1)=2.33_(-0.18)^(+0.15) for q

关键词： black hole physics (stars:)gamma ray burst:general (cosmology:)dark matter

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Cu,N codoped carbon nanosheets encapsulating ultrasmall Cu nanoparticles for enhancing selective 1,2-propanediol oxidation

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Journal of Energy Chemistry 2024年第4期91卷 27-35页

作者： Yonghai Feng min Yu minjia meng Lei Liu Dewei Rao Institute for Advanced Materials School of Materials Science and EngineeringJiangsu UniversityZhenjiang 212013JiangsuChina School of Chemistry and Chemical Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina

In the selective oxidation of biomass-based 1,2-propanediol(PDO)with oxygen as the terminal oxidant,it is challenging to improve the lactic acid(LA)selectivity for nonnoble metal nanoparticles(NPs)due to their limited oxygen reduction rate and easy C-C *** the high economic feasibility of nonnoble metals,i.e.,Cu,in this work,copper and nitrogen codoped porous carbon nanosheets encapsulating ultrafine Cu nanoparticles(Cu@Cu-N-C)were developed to realize highly selective of PDO oxidation to *** carbon-encapsulated ultrasmall Cu^(0)NPs in Cu@Cu-N-C have high PDO dehydrogenation activity while N-coordinated Cu(Cu-N)sites are responsible for the high oxygen reduction ***,the performance of catalytic PDO conversion to LA is optimized by a proposed pathway of PDO→hydroxylacetone→lactaldehyde→***,the enhanced LA selectivity is 88.5%,and the PDO conversion is up to 75.1%in an O_(2)-pressurized reaction system(1.0 MPa O_(2)),superior to other Cu-based catalysts,while in a milder nonpressurized system(O_(2)flow rate of 100 mL min-1),a remarkable LA selectivity(94.2%)is obtained with 39.8%PDO conversion,2.2 times higher than that of supported Au nanoparticles(1%Au/C).Moreover,carbon encapsulation offers Cu@Cu-N-C with strong leaching resistance for better recycling.

关键词： Selective oxidation Copper and nitrogen doped carbon 1,2-Propanediol Ultrasmall Cu nanoparticles Lactic acid

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Neural network modeling and prediction of HfO2 thin film properties tuned by thermal annealing

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High Power Laser Science and Engineering 2024年第2期12卷 79-86页

作者： min Gao Chaoyi Yin Jianda Shao Meiping zhu School of Microelectronics Shanghai UniversityShanghaiChina Laboratory of Thin Film Optics Key Laboratory of Materials for High Power LaserShanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghaiChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijingChina Hangzhou Institute for Advanced Study University of Chinese Academy of SciencesHangzhouChina

Plasma-enhanced atomic layer deposition (PEALD) is gaining interest in thin films for laser applications, and post-annealing treatments are often used to improve thin film properties. However, research to improve thin film properties is often based on an expensive and time-consuming trial-and-error process. In this study, PEALD-HfO2 thin film samples were deposited and treated under different annealing atmospheres and temperatures. The samples were characterized in terms of their refractive indices, layer thicknesses and O/Hf ratios. The collected data were split into training and validation sets and fed to multiple back-propagation neural networks with different hidden layers to determine the best way to construct the process–performance relationship. The results showed that the three-hidden-layer back-propagation neural network (THL-BPNN) achieved stable and accurate fitting. For the refractive index, layer thickness and O/Hf ratio, the THL-BPNN model achieved accuracy values of 0.99, 0.94 and 0.94, respectively, on the training set and 0.99, 0.91 and 0.90, respectively, on the validation set. The THL-BPNN model was further used to predict the laser-induced damage threshold of PEALD-HfO2 thin films and the properties of the PEALD-SiO2 thin films, both showing high accuracy. This study not only provides quantitative guidance for the improvement of thin film properties but also proposes a general model that can be applied to predict the properties of different types of laser thin films, saving experimental costs for process optimization.

关键词： laser-induced damage threshold laser thin film neural network plasma-enhanced atomic layer deposition

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Aerodynamic Performance of Three Flapping Wings with Unequal Spacing in Tandem Formation

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Journal of Bionic Engineering 2024年第4期21卷 1662-1676页

作者： min Chang Ziyi Xu Zengshuang Chen Li Li Xueguang meng Unmanned System Research Institute National Key Laboratory of Unmanned Aerial Vehicle TechnologyNorthwestern Polytechnical UniversityXi’an710072People’s Republic of China State Key Laboratory for Strength and Vibration of Mechanical Structures School of Aerospace EngineeringXi’an Jiaotong UniversityXi’an710049People’s Republic of China Xi’an Aeronautics Computing Technique Research Institute Aviation Industry Corporation of ChinaXi’an710076People’s Republic of China

To better understand the aerodynamic reasons for highly organized movements of flying organisms,the three-flapping wing system in tandem formation was studied numerically in this *** from previous relevant studies on the multiple flapping wings that are equally spaced,this study emphasizes the impact of unequal spacing between individuals on the aerodynamics of each individual wing as well as the whole *** is found that swapping the distance between the first and second wing with the distance between the second wing and the rearmost wing does not affect the overall aerodynamic performance,but significantly changes the distribution of aerodynamic benefits across each *** the whole flapping cycle,three effects are at *** narrow channel effect and the downwash effect can promote and weaken the wing lift,respectively,while the wake capture effect can boost the *** also shows that these effects could be manipulated by changing the spacing between adjacent *** findings provide a novel way for flow control in tandem formation flight and are also inspiring for designing the formation flight of bionic aircraft.

关键词： Three flapping wings Unequal spacing Aerodynamic performance Tandem formation

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Generation and expression analysis of BAC humanized mice carrying HLA-DP401 haplotype

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Animal Models and Experimental Medicine 2021年第2期4卷 116-128页

作者： Feng Li meng-min zhu Bo-wen Niu Ling-ling Liu Xiu-hua Peng Hua Yang Bo-yin Qin Meixiang Wang Xiaonan Ren Xiaohui Zhou Department of Laboratory Animal Science Shanghai Public Health Clinical CenterFudan UniveristyShanghaiChina Department of Scientific Research Shanghai Public Health Clinical CenterFudan UniveristyShanghaiChina

Background:Human leukocyte antigen(HLA)-DP is much less studied than other HLA class Ⅱ antigens,that is,HLA-DR and HLA-DQ,***,the accumulating data have suggested the important roles of DP-restricted responses in the context of cancer,allergy,and infectious *** of animal models expressing these genes as authentic cis-haplotypes blocks our understanding for the role of HLA-DP haplotypes in ***:To explore the potential cis-acting control elements involved in the tran-scriptional regulation of the HLA-DPA1/DPB1 gene,we performed the expression analysis using bacterial artificial chromosome(BAC)-based transgenic humanized mice in the C57BL/6 background,which carried the entire HLA-DP401 gene *** further developed a mouse model of Staphylococcus aureus pneumonia in HLA-DP401 humanized transgenic mice,and performed the analysis on the expres-sion pattern of HLA-DP401 and immunological responses in the ***:In this study,we screened and identified a BAC clone spanning the entire HLA-DP gene *** from this clone was analyzed for integrity by pulsed-field gel electrophoresis and then microinjected into fertilized mouse oocytes to produce transgenic founder *** sets of PCR primers for regional markers with an average distance of 15 kb between each primer were used to confirm the integrity of the transgene in the five transgenic lines carrying the HLA-DPA1/DPB1 *** copy numbers were determined by real-time PCR ***-DP401 gene expression was analyzed at the mRNA and protein *** infection with S aureus Newman did not alter the percentage of immune cells in the spleen and thymus from the HLA-DP401-H2-Aβ1 humanized *** expression of HLA-DP401 was observed in the thymus of the humanized mice infected by S ***:We generated several BAC transgenic mice,and analyzed the expres-sion of HLA-DPA1/DPB1 in those mice.A model of S aureus-induced pneumonia in the HLA-DP401-H2-Aβ1^

关键词： bacterial artificial chromosome(BAC) gene expression HLA-DP4 humanized mice Staphylococcus aureus pneumonia

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