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肝细胞生长因子及其受体基因在大鼠肝脏癌变过程中的表达

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外科理论与实践 1998年第1期3卷 33-35页

作者：罗运权吴孟超陈汉第二军医大学东方肝胆外科医院 200433

目的:了解肝细胞生长因子(HGF)及其受体基因在大鼠肝脏癌变过程中的表达变化规律,以及这些变化与肿瘤生物学行为的关系.方法:以二乙基亚硝胺(DENA)诱发的大鼠肝癌模型为对象,采用地高辛标记的HGF及其受体DNA探针,检测HGF及其受体基因在... 详细信息

目的:了解肝细胞生长因子(HGF)及其受体基因在大鼠肝脏癌变过程中的表达变化规律,以及这些变化与肿瘤生物学行为的关系.方法:以二乙基亚硝胺(DENA)诱发的大鼠肝癌模型为对象,采用地高辛标记的HGF及其受体DNA探针,检测HGF及其受体基因在大鼠肝脏癌变过程中的表达.结果:HGF及其受体基因在大鼠肝脏组织中均有表达,但在诱癌的不同时期,其表达阳性率差异明显,HGF表达阳性率在肝硬变前期达最高,肝硬变期次之,癌变期与正常对照相比差异无显著意义.HGF受体基因表达阳性率随肝组织逐渐癌变呈逐渐增加趋势,以癌变期为最高,正常肝组织中未见表达.结论:HGF及其受体基因过量表达与大鼠肝脏癌变的演化过程关系密切,HGF及其受体系统对调节肿瘤生长、转移起重要作用.

关键词：肝肿瘤肝细胞生长因子受体基因表达

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非体外静脉转流猪原位肝移植术模型的研究

苏州大学学报（医学版） 2008年第6期28卷 933-936页

作者：邵文雨朱楠张海斌杨宁高广勇钱海鑫杨广顺第二军医大学东方肝胆外科医院胆二科上海200433 苏州大学附属第一医院普外科江苏苏州215006

目的探讨非体外静脉转流猪原位肝移植术模型的可行性。方法选用太湖猪种的梅山猪行无体外静脉转流条件猪原位肝移植术,观察供/受体手术情况;分别于无肝前期、无肝期、新肝期和关腹前进行血气分析和血电解质、凝血功能和部分生化指标测定... 详细信息

目的探讨非体外静脉转流猪原位肝移植术模型的可行性。方法选用太湖猪种的梅山猪行无体外静脉转流条件猪原位肝移植术,观察供/受体手术情况;分别于无肝前期、无肝期、新肝期和关腹前进行血气分析和血电解质、凝血功能和部分生化指标测定,记录平均动脉压、中心静脉压、心率,采用前后对照的方法,明确非体外静脉转流猪原位肝移植术不同时期主要存在的病理生理改变,探讨相应的处理原则。结果顺利完成非体外静脉转流猪原位肝移植术15例(手术成功率88.2%),受体肝移植手术过程中各重要生理指标变化较大,经积极有效的针对性处理后,除关腹前APTT和Fg仍异常(P<0.05)外,其他都恢复至基础水平。结论良好的手术技术,积极有效地防止和纠正内环境改变,是稳定建立非静脉转流猪原位肝移植术模型的关键因素;该模型与临床肝移植术的方法及要求基本一致,有很强的模拟性。

关键词：体外静脉转流原位肝移植术无肝期再灌注后综合征猪

多层螺旋CT:三期薄层扫描发现微小肝癌病灶

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医学影像学杂志 2003年第4期13卷 251-254页

作者：程红岩徐爱民陈栋贾雨辰上海第二军医大学东方肝胆外科医院放射科上海200438

目的 :研究多层螺旋CT肝脏三期扫描 (Triple phasehelicalCT ,THCT)在发现微小肝癌病灶的作用 ,提高检出率 ,避免不必要的手术切除而导致的术后复发。方法 :选择原诊断为单发性肝癌并可手术者 2 0 0例 ,前瞻性薄层THCT ,即平扫、动脉期 ... 详细信息

目的 :研究多层螺旋CT肝脏三期扫描 (Triple phasehelicalCT ,THCT)在发现微小肝癌病灶的作用 ,提高检出率 ,避免不必要的手术切除而导致的术后复发。方法 :选择原诊断为单发性肝癌并可手术者 2 0 0例 ,前瞻性薄层THCT ,即平扫、动脉期 (2 4～ 2 5s)、门静脉期 (45～ 5 0s)和延迟期 (2～ 3min) ,对比剂为非离子型 (1.5ml/kg) ,注射速率为 3ml/s。发现5 4例患者 (2 7% ,5 4 / 2 0 0 )在主病灶周围或肝内有多发的微小病灶 (<10mm)。CT图像在PACS的网络工作站上 ,采用Rad worksTM5 .1版本软件 ,由 3名放射科医师分别在各自的工作站上观察分析。在CT检查后数天 ,行TACE(transcatherarterialchemoembolization ,TACE)。结果 :在 5 4例病例中 ,共发现微小病灶 2 70枚 ,平均 5枚。CT特点是平扫呈等密度或低密度 ,增强扫描动脉期呈等密度或高密度 ;门静脉期表现为高密度、等密度或低密度 ;延迟期呈等密度或低密度。敏感性分别为 :动脉期 91.85 % ,门静脉期 85 .5 6 %和延迟期 88.89% ,综合三期扫描敏感性为 93.33%。DSA上发现微小病灶的敏感性为 80 .74 %。结论 :薄层THCT扫描对发现肝内微小病灶是必须的 ,显示微小病灶以动脉期和延迟期为佳。

关键词：多层螺旋CT 三期薄层扫描微小肝癌病灶

Cancer stem cell-immune cell crosstalk in the tumor microenvironment for liver cancer progression

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Frontiers of Medicine 2024年第3期18卷 430-445页

作者： Yue Ma Hongwei Lv Fuxue Xing Wei Xiang Zixin wu Qiyu Feng Hongyang Wang Wen Yang Cancer Research Center First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei230027China National Center for Liver Cancer Naval Medical University(Second Military Medical University)Shanghai201805China International Co-operation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery HospitalNaval Medical University(Second Military Medical University)Shanghai200438China shanghai Key Laboratory of Hepato-biliary Tumor Biology Shanghai200438China Key Laboratory of Signaling Regulation and Targeting Therapy of Liver Cancer Ministry of EducationShanghai200438China

Crosstalk between cancer cells and the immune microenvironment is determinant for liver cancer progression.A tumor subpopulation called liver cancer stem cells(CSCs)significantly accounts for the initiation,metastasis,therapeutic resistance,and recurrence of liver *** evidence demonstrates that the interaction between liver CSCs and immune cells plays a crucial role in shaping an immunosuppressive microenvironment and determining immunotherapy *** review sheds light on the bidirectional crosstalk between liver CSCs and immune cells for liver cancer progression,as well as the underlying molecular mechanisms after presenting an overview of liver CSCs characteristic and their ***,we discuss the potential application of liver CSCs-targeted immunotherapy for liver cancer treatment.

关键词： liver cancer cancer stem cell immune cell immunotherapy

Protective effect of prednisolone on ischemia-induced liver injury in rats

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World Journal of Gastroenterology 2008年第27期14卷 4332-4337页

作者： meng Wang Feng Shen Le-Hua Shi Tao Xi Xi-Feng Li Xu Chen meng-chao wu eastern hepatobiliary surgery hospital Second Military Medical University 225 Changhai Road Shanghai 200438 China

AIM: To investigate the effects of prednisolone on cell membrane bleb formation, calpain μ activation and talin degradation during hepatic ischemia-reperfusion injury in rats. METHODS: The hilar area of the left lateral and median lobes of rat liver (68%) was clamped for 60 min and followed by 120 min reperfusion. Prednisolone was administered at 1.0, 3.0, or 10 mg/kg at 30 min before ischemia. In addition to biochemical and microscopic analyses, activation of calpain μ was determined using specific antibodies against the intermediate (activated) form of calpain μ. Degradation of talin was also studied by Western blotting. RESULTS: In the control and prednisolone (1.0 mg/kg) groups, serum aspartate transaminase (AST) and alanine transaminase (ALT) level were elevated, and cell membrane bleb formation was observed after 120 min of reperfusion. Moreover, calpain μ activation and talin degradation were detected. Infusion of prednisolone at 3.0 or 10 mg/kg significantly suppressed serum AST and ALT, and prevented cell membrane bleb formation. At 10 mg/kg, prednisolone markedly suppressed calpain μ activation and talin degradation. CONCLUSION: Prednisolone can suppress ischemia- reperfusion injury of the rat liver. Its cytoprotective effect is closely associated with the suppression of calpain μ activation and talin degradation.

关键词： Ischemia-reperfusion Prednisolone Cell membrane bleb Calpain μ Talin

Surgicopathological classification of hepatic space-occupying lesions:A single-center experience with literature review

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World Journal of Gastroenterology 2011年第19期17卷 2372-2378页

作者： Wen-Ming Cong Hui Dong Lu Tan Xu-Xu Sun meng-chao wu Department of Pathology Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China Department of surgery Eastern Hepatobili- ary Surgery Hospital Second Military Medical University 225 Changhai Road Shanghai 200438 China

Accompanying rapid developments in hepatic surgery,the number of surgeries and identifications of histological types of primary hepatic space-occupying lesions (PHSOLs) have increased *** has led to many changes in the surgicopathological spectrum of PHSOLs,and has contributed to a theoretical basis for modern hepatic surgery and oncological *** 1982 and 2009 at the eastern hepatobiliary surgery hospital (EHBH) in shanghai,31 901 patients underwent surgery and were diagnosed as having a *** this paper,we present an analysis of the PHSOL cases at the EHBH for this time period,along with results from a systematic literature *** describe a surgicopathological spectrum comprising more than 100 types of PHSOLs that can be stratified into three types:tumor-like,benign,and *** also stratified the PHSOLs into six subtypes derived from hepatocytes;cholangiocytes;vascular,lymphoid and hemopoietic tissues;muscular,fibrous and adipose tissues;neural and neuroendocrine tissues;and miscellaneous *** present study provides a new classification system that can be used as a current reference for clinicians and pathologists to make correct diagnoses and differential diagnoses among various PHSOLs.

关键词： Liver tumors Tumor-like lesions Pathology Immunohistochemistry Classification

Precautions in caudate lobe resection: Report of 11 cases

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World Journal of Gastroenterology 2008年第17期14卷 2767-2770页

作者： Zeng-Qing Wen Yi-Qun Yan Jia-Mei Yang meng-chao wu First Department of Hepatic surgery Eastern HepatobiliarySurgery Hospital Second Military Medical University Shanghai200438 China

AIM： To find the precautions against the safety in caudate lobe resection. METHODS： The clinical data obtained from 11 cases of primary liver cancer in caudate lobe who received hepatectomy successfully were retrospectively analyzed. Four safe procedures were used in resection of primary liver cancer in caudate lobe：（1） selection of appropriate skin incision to obtain excellent exposure of operative field; （2） adequate mobilization of the liver to allow the liver to be displaced upwards to the left or to the right; （3） preparatory placement of tapes for total hepatic vascular exclusion, so that this procedure could be used to control the fatal bleeding of the liver when necessary; （4） selection of the ideal route for hepatectomy based on the condition of the tumor and the combined removal of multiple lobes if necessary. Among the 11 cases, simple occlusion of vessels of porta hepatis was used in caudate Iobectomy for 6 cases, while in the other cases, the vessels were intermittently occluded several times or total hepatic vascular isolation was used in the caudate Iobectomy. Combined partial right hepatectomy was done for 3 cases, combined left lateral Iobectomy for 2 cases and caudate Iobectomy alone for 6 cases. RESULTS： Operation was successful for all the 11 cases. Intermittent inflow occlusion was performed for all patients for 15 min at 5-min intervals. Blockade was performed twice in 3 patients and total hepatic vascular exclusion was performed in one of the three patients. Blockade was performed three times in one patient, including a total hepatic vascular exclusion. Total hepatic vascular exclusion was performed only in one patient. The mean blood loss was 300 mL. Ascites and pleural effusion occurred in 4 patients, jaundice in 1 patient. Six patients died of tumor recurrence in 6, 11, 12, 13, 15, 19 mo after operation, respectively. The other 5 patients have survived more than 16 mo since the operation. CONCLUSION： Caudate Iobectomy for liver cance

关键词： Caudate lobe Primary liver cancer Hepatectomy Porta hepatis Vascular exclusion

Identification of a novel ankylosing spondylitis risk allele (-857T) of the tumor necrosis factor-alpha in Chinese Han population

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Identification of a novel ankylosing spondylitis risk allele...

Predictors and clinical outcomes for spontaneous rupture of hepatocellular carcinoma

中国遗传学会七届一次青年研讨会暨上海高校模式生物E——研究院第一届模式生物学术研讨会

作者： Chen Rui-Wen, Duan Shi-Wei. Cai Qing, wu Dan, Lin Jin-An, Lv Ke, Hu Jian-Da, Shi Ke , Zhang Gong-Liang , Han Xing-Hai, Chen Qi, Feng Guo-Yin, He Lin, and Sun Shu-Han (Department of medical Genetics, College of Basic medical Sciences and Department of Rheumatology and Immunology, Changhai hospital, second military medical university, shanghai 200433, china,Bio-X Life Science Research Center, shanghai Jiao Tong university, shanghai 200030, china Institute of Nutrition Science, Chinese Academy of Sciences, shanghai 200031, china,Center of research,First Affiliated hospital and Fujian Institute of Hematology, Union hospital of Fujian medical university, Fuzhou 350005, china,Department of Immunogenetics, shanghai (Red Cross) Blood Center, shanghai 200051, china,shanghai Institute of Mental Health, 600 South Wan Ping Road, shanghai 200030, china)

The location of tumor necrosis factor-alpha gene (TNFα) at a susceptibility locus for ankylosing spondylitis (AS) on chromosome 6p21.3, has been identified by several linkage studies across multiple population groups. Long-term data on continuous anti-TNF therapy has confirmed that it is a safe and efficacious treatment for AS. In the light of these findings, we have carried out case-control studies in two independent Chinese AS cohorts (eastern Chinese, Southeastern Chinese). Both have revealed a novel risk allele (-857T) with AS (best p= 5.12E-21 and 2.32E-11 for eastern Chinese and Southeastern Chinese, respectively). Further association studies have suggested that -857T significantly increases the susceptibility to AS in both the HLA-B27-positive and HLA-B27-negative groups (global p = 0.0062 and 0.0065, respectively). The genotype-relative-risk analyses have indicated that the minor allele-containing (TT+TC) genotypes increase AS susceptibility to a greater degree than the homozygous-major-allele (CC) genotype frequency in cases of-857C>T (TT + CT vs. CC, p=0.0092 and 0.0025 respectively, in the HLA-B27 positive and negative groups). Single-SNP analyses as well as haplotype analyses have revealed that -857C>T appears to be the most likely variants underlying the association of the TNFa region with AS independently of HLA-B27.

关键词： Ankylosing Spondylitis TNFα polymorphism promoter HLA-B27 independent association

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World Journal of Gastroenterology 2012年第48期18卷 7302-7307页

作者： Qian Zhu Jing Li Jian-Jun Yan Liang Huang meng-chao wu Yi-Qun Yan Department of hepatobiliary surgery Jingmen First People's Hospital First Department of Hepatic surgery Eastern Hepatobiliary Surgery HospitalSecond Military Medical University

AIM:To determine the risk factors for hepatocellular carcinoma(HCC) rupture,and report the management and long-term survival results of patients with spontaneous rupture of ***:Among 4209 patients with HCC who were diagnosed at eastern hepatobiliary surgery hospital from April 2002 to November 2006,200(4.8%) patients with ruptured HCC(case group) were studied retrospectively in term of their clinical characteristics and prognostic *** one-stage therapeutic approach to manage ruptured HCC consisted of initial management by conservative treatment,transarterial embolization(TACE) or hepatic *** of various treatments in the case group were evaluated and compared with the control group(202 patients) without ruptured HCC during the same study *** data were expressed as mean ± SD or me-dian(range) where appropriate and compared using the unpaired t *** variables were compared using the Chi-square test with Yates correction or the Fisher exact test where *** overall survival rate in each group was determined using the Kaplan-Meier method and a log-rank ***:Compared with the control group,more patients in the case group had underlying diseases of hypertension(7.5% vs 3.0%,P =0.041) and liver cirrhosis(87.5% vs 56.4%,P 5 cm(83.0% vs 57.4%,P 5 cm(P < 0.001),vascular thrombus(P = 0.002) and extrahepatic invasion(P < 0.001) were predictive for spontaneous rupture of *** the 200 patients with spontaneous rupture of HCC,105 patients underwent hepatic resection,33 received TACE,and 62 were managed with conservative *** median survival time(MST) of all patients with spo

关键词： Hepatocellular carcinoma Spontaneous rupture Predictors Hepatectomy Overall survival Disease-free survival