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Knocking-Out OsPDR7 Triggers Up-Regulation of OsZIP9 Expression and Enhances Zinc Accumulation in Rice

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Rice science 2023年第1期30卷 36-49页

作者： meng Lu tanG Mingfeng ZHU Yuxing tan Longtao Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of Sciences Beijing 100049China Institute of Agricultural Quality Standard and Testing Technology Chongqing Academy of Agricultural SciencesChongqing 401329China

Zinc(Zn) is an essential trace mineral that is required for plant growth and development. A number of protein transporters, which are involved in Zn uptake, translocation and distribution, are finely regulated to maintain Zn homeostasis in plant. In this study, we functionally characterized an ATP-binding cassette(ABC) transporter gene, OsPDR7, which is involved in Zn homeostasis. Os PDR7 encodes a plasma membrane-localized protein that is expressed mainly in the exodermis and xylem in the rice ***7 mutants resulted in higher Zn accumulation compared with the wild type. Heterogeneous expression of OsPDR7 in a yeast mutant rescued the Zn-deficiency phenotype, implying transport activity of OsPDR7 to Zn in yeast. However, no ZIP genes except for OsZIP9 showed change in expression profile in the ospdr7 mutants, which suggested that OsPDR7 maintains cellular Zn homeostasis through regulating Os ZIP9 expression. RNA-Seq analysis further revealed a set of differentially expressed genes between the wild type and ospdr7 mutants that allowed us to propose a possible OsPDR7-associated signaling network involving transporters, hormone responsive genes, and transcription factors. Our results revealed a novel transporter involved in the regulation of Zn homeostasis and will pave the way toward a better understanding of the fine-tuning of gene expression in the network of transporter genes.

关键词： OsPDR7 OsZIP9 zinc metal accumulation rice ATP-binding cassette transporter

A novel two-stage Dissolved Gas Analysis fault diagnosis system based semi-supervised learning

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High Voltage 2022年第4期7卷 676-691页

作者： Xuemin tan chao Guo Ke Wang Fu Wan College of Control Engineering Chengdu University of Information TechnologyChengduChina State Grid Chengdu Power Supply Company ChengduChina China Electric Power Research Institute BeijingChina State Key Laboratory of Power Transmission Equipment and System Security and New Technology Chongqing UniversityChongqingChina

Dissolved Gas Analysis(DGA)is an important method for oil-immersed transformer fault ***,collecting labelled DGA data is difficult because the determi-nation of the transformer fault is time-consuming and expensive in the transformer substation,but DGA data without labels is easier to ***,the paper pro-posed a semi-supervised two-stage diagnostic system based DGA by using less labelled *** two-stage system includes a novel semi-supervised feature selection based Genetic Algorithm(GA)and Support Vector Machine(SVM)model(SSL-FS-GASVM)for selecting optimal features and a novel semi-supervised transformer fault diagnosis model based improved Artificial Fish Swarm Algorithm(AFSA)and SVM(SSL-IAFSA-SVM)for optimising the SVM ***,the performances of SSL-FS-GASVM and SSL-IAFSA-SVM models are tested and compared with traditional supervised diagnostic models combined with other optimisation methods,*** results show that the proposed two-stage system works in optimising features and parameters and has strong robustness in solving small sample classification problems.

关键词： fault diagnosis system

Synthesis, Characterization and Cytotoxicity of Ammine/Methylamine Platinum(Ⅱ) Complexes with Carboxylates

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Chemical Research in Chinese Universities 2006年第3期22卷 278-282页

作者： ZHANG Jin-chao SHEN Yong YANG meng-su Department of Chemistry College of Chemistry ＆ Environmental Science Hebei University Baoding 071002 P. R. China Department of Biology and Chemistry City University of Hong Kong Hong Kong P. R. China

Seven novel platinum （Ⅱ） complexes [ Pt（Ⅱ）（NH3）（CH3NH2）X2] （Ⅰ-Ⅶ ）（ X： CH3COO^-, CH2CICOO^- , CHCI2COO^- , C6H5-COO^- , p-CH3O--C6H4-COO^- , p-NH2-C6H4-COO^- , p-NO2--C6H4- COO^-） were prepared and characterized by means of elemental analysis, molar conductivity, thermal analysis, IR, UV, and ^1H NMR spectrometries. The cytotoxicity against HCT-8, BGC-823, MCF-7, EJ, and HL-60 cell lines increases in the following sequence： cisplatin 〉 Ⅳ 〉 Ⅴ 〉 Ⅵ 〉 Ⅶ 〉 Ⅰ 〉 Ⅱ 〉 Ⅲ. Moreover, the complexes （ Ⅰ --Ⅻ） display substantially greater activities agaist EJ and HL-60 cell lines than those against the cell lines from other carcinomas. They can induce a concentration-dependent accumulation of HL-60 cells in the G2/M phase of the cell cycle as cisplatin. There is no significant correlation between total DNA platination levels and cytotoxicity of the complexes.

关键词： Ammine/methylamine platinum(Ⅱ) complex Synthesis Cytotoxicity Cell cycle: DNA-binding

Microglia in neurodegenerative diseases: mechanism and potential therapeutic targets

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Signal Transduction and Targeted Therapy 2023年第10期8卷 4746-4782页

作者： chao Gao Jingwen Jiang Yuyan tan Shengdi Chen Department of Neurology Rujin HospitalShanghai Jiao Tong University School of Medicine200025 ShanghaiChina Lab for Translational Research of Neurodegenerative Diseases Shanghai Institute for Advanced Immunochemical Studies(SIAIS)Shanghai Tech University201210 ShanghaiChina

Microglia activation is observed in various neurodegenerative *** advances in single-cell technologies have revealed that these reactive microglia were with high spatial and temporal *** identified microglia in specif... 详细信息

关键词： degenerative heterogeneity functions

Geochemical speciation and spatial distributions of phosphorus in surface sediments from the basin of the Marcus-Wake seamounts in the western Pacific Ocean

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Acta Oceanologica Sinica 2022年第4期41卷 80-90页

作者： chao Yuan Fansheng meng Xuying Yao Jianyu Ni Key Laboratory of Submarine Geosciences Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China

The concentrations of five forms of phosphorus(P)including exchangeable or loosely adsorbed P(Ex-P),Febound P(Fe-P),authigenic P(Auth-P),detrital P(Det-P),and organic P(Org-P)from the basin among the Marcus-Wake seamounts(19.4°–24°N,156.5°–161.5°E)in the western Pacific Ocean were quantified using a sequential extraction method(SEDEX)to investigate the distribution and sources of different P *** of total P(TP)varied from 14.0μmol/g to 44.1μmol/g,with an average of(32.4±7.7)μmol/*** phosphorus,which was the major chemical form of sedimentary P,ranged from 12.6μmol/g to 40.6μmol/g,while the concentration of Org-P varied between 1.38μmol/g and 5.18μmol/g,accounting for 83.4%–93.4%and 6.6%–16.6%of the TP,*** relative proportions of the five P species followed the order of Det-P>Auth-P>Org-P>Fe-P>*** average,Det-P was the major P sink resulted from the atmospheric input and accounted for approximately 58.9%±12.4%of the ***-P and Org-P comprised 22.8%±11.4%and11.5%±3.0%of the TP,respectively,while Fe-P accounted for 5.1%±2.6%.Lastly,Ex-P comprised 1.6%±0.3%of the ***-P exhibited a negative correlation with Fe-P and Auth-P,while Fe-P showed a positive correlation with *** indicated that the formation of Fe-P and Auth-P was at the expense of the regeneration or remineralization of Org-P during early *** concentrations of Det-P and Auth-P as well as a low ratio of total organic C to reactive P(TOC/Rea-P)suggested that the aeolian input may play a significant role in sedimentary P budget in the study ***,well-oxygenated bottom water and low sedimentation rate could be responsible for the low TOC/Org-P ratio in the sediment.

关键词： phosphorus speciation SEDEX sediments western Pacific Ocean

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法国英语教材价值观呈现研究

外语学刊 2025年第1期 78-85页

作者：谈佳北京外国语大学法语语言文化学院北京100089

本文梳理了法国阿歇特出版社中学英语教材Teen Time的价值观呈现方式。研究发现,教材选择呈现的价值元素,不仅对应欧洲价值观调查揭示的法国人坚持的价值观,同时也是英语素材所承载的目的语国家所认同的价值观,反复出现的“社团活动、... 详细信息

本文梳理了法国阿歇特出版社中学英语教材Teen Time的价值观呈现方式。研究发现,教材选择呈现的价值元素,不仅对应欧洲价值观调查揭示的法国人坚持的价值观,同时也是英语素材所承载的目的语国家所认同的价值观,反复出现的“社团活动、规则意识、文化多样性和生态保护”,反映出在与他人、与社会以及与自然关系上法国与英语国家的共同态度和期许。Teen Time通过标题引领、图文呼应和文化板块拓展深入的呈现方式,传递价值观,并设置层层任务,让学习者在行动中体验,将价值观内化于心、外化于行,实现外语教学的育人功能,其价值观的选取角度和呈现方式对我国中学外语教材的编写具有一定的借鉴意义。

关键词：价值观呈现方式法国英语教材中学行动中体验

Solid oxide fuel cells in combination with biomass gasification for electric power generation

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Chinese Journal of Chemical Engineering 2020年第4期28卷 1156-1161页

作者： Huangang Shi Qianjun Li Wenyi tan Hao Qiu chao Su School of Environmental Engineering Nanjing Institute of TechnologyNanjing 211167PR China School of Energy and Power Engineering Jiangsu University of Science and TechnologyZhenjiang 212003PR China WA School of Mines MineralsEnergy and Chemical Engineering(WASM-MECE)Curtin UniversityGPO Box U1987PerthWA 6845Australia

Biomass,a source of renewable energy,represents an effective substitute to fossil *** is a process that organics are thermochemically converted into valuable gaseous products(***).In this work,the catalytic test demonstrated that the biogas produced from biomass gasification mainly consists of H2,CH4,CO,and CO2,which were then be used as the fuel for solid oxide fuel cell(SOFC).Planar SOFCs were fabricated and *** steam reforming of biogas was carried out at the anode of a SOFC to obtain a hydrogen-rich *** performance of the SOFCs operating on generated biogas was investigated by I-V polarization and electrochemical impedance spectra *** excellent cell performance was obtained,for example,the peak power density of the SOFC reached 1391 mW·cm-2 at 750℃when the generated biogas was used as the ***,the SOFC fuelled by simulated biogas delivered a very stable operation.

关键词： Biomass gasification Biogas Solid oxide fuel cell Steam reforming

A review of bird-like flapping wing with high aspect ratio

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Chinese Journal of Aeronautics 2023年第1期36卷 22-44页

作者： Changchuan XIE Nongyue GAO Yang meng Yue WU chao YANG School of Aeronautic Science and Engineering Beihang UniversityBeijing 100191China

Bird-like flapping-wing vehicles with a high aspect ratio have the potential to fulfill missions given to micro air vehicles,such as high-altitude reconnaissance,surveillance,rescue,and bird group guidance,due to their good loading and long endurance *** and aeronautical researchers have explored the mystery of avian flight and made efforts to reproduce flapping flight in bioinspired aircraft for ***,the cognitive depth from theory to practice is still very *** mechanism of generating sufficient lift and thrust during avian flight is still not fully *** wings with unique biological structures such as feathers make modeling,simulation,experimentation,and analysis much more *** paper reviews the research progress on bird-like flapping wings from flight mechanisms to *** used numerical computing methods are briefly *** aeroelastic problems are also *** results of the investigation show that a leading-edge vortex can be found during avian *** induction and maintenance may have a close relationship with wing configuration,kinematics and *** present models of flapping wings are mainly two-dimensional airfoils or three-dimensional single root-jointed geometric plates,which still exhibit large differences from real bird *** is encouraged to consider the nonignorable effect on aerodynamic performance due to large-scale nonlinear *** appropriate flexibility can improve the peak values and efficiencies of lift and thrust,but the detailed conclusions always have strong background dependence.

关键词： Aeroelasticity Bird-like flapping wing Flapping motion High aspect ratio Modeling Nonlinear

A deep learning-based binocular perception system

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Journal of Systems Engineering and Electronics 2021年第1期32卷 7-20页

作者： SUN Zhao MA chao WANG Liang meng Ran PEI Shanshan Faculty of Information Technology Macao University of Science and TechnologyMacao 999078China Baidu’s Intelligent Driving Group Beijing 100193China Beijing Smarter Eye Technology Co. Ltd.Beijing 100023China

An obstacle perception system for intelligent vehicle is *** proposed system combines the stereo version technique and the deep learning network model,and is applied to obstacle perception tasks in complex *** this paper,we provide a complete system design project,which includes the hardware parameters,software framework,algorithm principle,and optimization *** addition,special experiments are designed to demonstrate that the performance of the proposed system meets the requirements of actual *** experiment results show that the proposed system is valid to both standard obstacles and non-standard obstacles,and suitable for different weather and lighting conditions in complex *** announces that the proposed system is flexible and robust to the intelligent vehicle.

关键词： intelligent vehicle stereo matching deep learning environment perception