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阿兹夫定体外抗登革病毒活性研究

药学学报 2018年第6期53卷 944-949页

作者：张春涛罗荣华陈欢刘光明常俊标杨柳萌郑永唐大理大学药学与化学学院云南大理671000 中国科学院昆明动物研究所云南省活性多肽研究与利用重点实验室/中国科学院动物模型与人类疾病重点实验室云南昆明650223 河南师范大学河南新乡453007

本文通过检测化合物对登革Ⅱ型重组病毒(DENV-ⅡLuc+)表达海肾荧光素酶活性的影响,进行化合物筛选,发现阿兹夫定(azvudine,FNC)、盐酸阿兹夫定(hydrochloride salt of azvudine,FNC-HCl)和三磷酸酯阿兹夫定(triphosphate azvudine,FNC-TP)能够抑制DENV-ⅡLuc+的复制,并研究其体外抗登革病毒(dengue virus,DENV)活性。首先通过噬斑法检测FNC、FNC-HCl和FNC-TP对DENV的抑制活性;并通过蛋白印迹实验检测3种化合物对DENV-Ⅱ囊膜蛋白E(envelope protein E)表达的影响,最后通过实时定量PCR检测化合物对DENV-Ⅱ病毒RNA的抑制作用。结果显示,FNC、FNC-HCl和FNC-TP抑制DENV复制的半数有效浓度(EC50)在0.54~2..42.mol·L^(-1)之间,阳性药物利巴韦林抑制DENV-Ⅱ的EC50为40.78±1.02.mol·L^(-1)。蛋白印迹实验及定量结果显示,3种化合物均能抑制DENV-Ⅱ囊膜蛋白E的表达,并且显著地抑制DENV-Ⅱ病毒RNA的复制。通过MTT法检测发现FNC、FNC-HCl和FNC-TP的细胞毒性很小,半数细胞毒性浓度(CC50)均大于3 000.00μmol·L^(-1)。本研究表明FNC、FNC-HCl和FNC-TP在细胞水平、蛋白水平及RNA水平均能抑制DENV的复制,且抑制作用优于利巴韦林,有望成为抗登革病毒的候选药物。

关键词：阿兹夫定登革病毒抗病毒活性体外

Species-specific and generalized allometric biomass models for eight Fagaceae species in the understory of evergreen broadleaved forests in subtropical China

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Journal of Forestry Research 2024年第4期35卷 156-170页

作者： Shengwang meng Qianyanzhou Ecological Research Station Key Laboratory of Ecosystem Network Observation and ModelingInstitute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101People’s Republic of China

Quantifying the biomass of saplings in the regeneration component is critical for understanding biogeochemical processes of forest ***,accurate allometric equations have yet to be developed in sufficient *** develop species-specific and generalized allometric equations,154 saplings of eight Fagaceae tree species in subtropical China’s evergreen broadleaved forests were *** dendrometric variables,root collar diameter(d),height(h),and crown area(ca)were applied in the model by the weighted nonlinear seemingly unrelated regression *** only d as an input variable,the species-specific and generalized allometric equations estimated the aboveground biomass reasonably,with R _(adj)^(2. values generally>*** h and/or ca improved the fitting of some biomass components to a certain *** equations showed a relatively large coefficient of variation but comparable bias to species-specific *** in the absence of species-specific equations at a given location are generalized equations for mixed species *** developed regression equations can be used to accurately calculate the aboveground biomass of understory Fagaceae regeneration trees in China’s subtropical evergreen broadleaved forests.

关键词： Allometry Aboveground biomass Additivity Regeneration Subtropical China

A vertical track nonlinear energy sink

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Applied Mathematics and Mechanics(English Edition) 2024年第6期45卷 931-946页

作者： meng LI Hu DING Shanghai Key Laboratory of Mechanics in Energy Engineering School of Mechanics and Engineering ScienceShanghai Frontier Science Center of MechanoinformaticsShanghai UniversityShanghai 200444China

Eliminating the effects of gravity and designing nonlinear energy sinks(NESs)that suppress vibration in the vertical direction is a challenging task with numerous damping *** this paper,the dynamic design of a vertical track nonlinear energy sink(VTNES)with zero linear stiffness in the vertical direction is proposed and realized for the first *** motion differential equations of the VTNES coupled with a linear oscillator(LO)are *** the strong nonlinearity considered of the VTNES,the steady-state response of the system is analyzed with the harmonic balance method(HBM),and the accuracy of the HBM is verified *** this basis,the VTNES prototype is manufactured,and its nonlinear stiffness is *** damping effect and dynamic characteristics of the VTNES are studied theoretically and *** results show that the VTNES has better damping effects when strong modulation responses(SMRs)***,even for small-amplitude vibration,the VTNES also has a good vibration suppression *** sum up,in order to suppress the vertical vibration,an NES is designed and developed,which can suppress the vertical vibration within certain ranges of the resonance frequency and the vibration intensity.

关键词： track nonlinear energy sink(TNES) vertical direction gravity nonlinear stiffness harmonic balance method(HBM) vibration control

Nested Saturated Control of Uncertain Complex Cascade Systems Using Mixed Saturation Levels

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1163-1174页

作者： meng Li Zhigang Zeng Wuhan National Laboratory for Optoelectronics Wuhan 430074 School of Artificial Intelligence and Automation Huazhong University of Science and TechnologyWuhan 430074China School of Artificial Intelligence and Automation Huazhong University of Science and TechnologyWuhan 430074 Key Laboratory of Image Processing and Intelligent Control of Education Ministry of China Wuhan 430074China

This study addresses the problem of global asymptotic stability for uncertain complex cascade systems composed of multiple integrator systems and non-strict feedforward nonlinear systems. To tackle the complexity inherent in such structures, a novel nested saturated control design is proposed that incorporates both constant saturation levels and state-dependent saturation levels. Specifically, a modified differentiable saturation function is proposed to facilitate the saturation reduction analysis of the uncertain complex cascade systems under the presence of mixed saturation levels. In addition, the design of modified differentiable saturation function will help to construct a hierarchical global convergence strategy to improve the robustness of control design scheme. Through calculation of relevant inequalities, time derivative of boundary surface and simple Lyapunov function,saturation reduction analysis and convergence analysis are carried out, and then a set of explicit parameter conditions are provided to ensure global asymptotic stability in the closed-loop systems. Finally, a simplified system of the mechanical model is presented to validate the effectiveness of the proposed method.

关键词： method saturation differentiable

Adaptive admittance tracking control for interactive robot with prescribed performance

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Journal of Systems Engineering and Electronics 2024年第2期35卷 444-450页

作者： meng Qingrui LIN Yan School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100190China College of Electrical Engineering and Automation Shandong University of Science and TechnologyQingdao 266590China

An adaptive control approach is presented in this paper for tracking desired trajectories in interactive manipulators. The controller design incorporates prescribed performance functions (PPFs) to improve dynamic performance. Notably, the performance of the output error is confined in an envelope characterized by exponential convergence, leading to convergence to zero. This feature ensures a prompt response from admittance control and establishes a reliable safety framework for interactions. Simulation results provide practical insights,demonstrating the viability of the control scheme proposed in this paper.

关键词： prescribed performance admittance control adaptive control robots

Cooperative User-Scheduling and Resource Allocation Optimization for Intelligent Reflecting Surface Enhanced LEO Satellite Communication

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China Communications 2024年第2期21卷 227-244页

作者： meng meng Bo Hu Shanzhi Chen Jianyin Zhang State Key Lab of Networking and Switching Technology Beijing University of Posts and TelecommunicationsBeijing 100876China State Key Lab of Wireless Mobile Communication China Academy of Telecommunication TechnologyBeijing 100191China Research Institute of China Mobile Beijing 100053China

Lower Earth Orbit(LEO) satellite becomes an important part of complementing terrestrial communication due to its lower orbital altitude and smaller propagation delay than Geostationary satellite. However, the LEO satellite communication system cannot meet the requirements of users when the satellite-terrestrial link is blocked by obstacles. To solve this problem, we introduce Intelligent reflect surface(IRS) for improving the achievable rate of terrestrial users in LEO satellite communication. We investigated joint IRS scheduling, user scheduling, power and bandwidth allocation(JIRPB) optimization algorithm for improving LEO satellite system *** optimization problem of joint user scheduling and resource allocation is formulated as a non-convex optimization problem. To cope with this problem, the nonconvex optimization problem is divided into resource allocation optimization sub-problem and scheduling optimization sub-problem firstly. Second, we optimize the resource allocation sub-problem via alternating direction multiplier method(ADMM) and scheduling sub-problem via Lagrangian dual method ***, we prove that the proposed resource allocation algorithm based ADMM approaches sublinear convergence theoretically. Finally, we demonstrate that the proposed JIRPB optimization algorithm improves the LEO satellite communication system throughput.

关键词： convex optimization intelligent reflecting surface LEO satellite communication OFDM

Identifying Comprehensive Genomic Alterations and Potential Neoantigens for Cervical Cancer Immunotherapy in a Cohort of Chinese Squamous Cell Carcinoma of the Cervix

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Biomedical and Environmental Sciences 2024年第6期37卷 565-580页

作者： meng Wu Jialu Zhou Zhe Zhang Yuanguang meng School of Medicine Nankai UniversityTianjin 300071China Department of Obstetrics and Gynecology The First Medical Center of Chinese PLA General HospitalBeijing 100853China Department of Obstetrics and Gynecology Chinese PLA Medical SchoolBeijing 100853China Department of Obstetrics and Gynecology The Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China

Objective Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma(CSCC).Methods Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC *** Sequencing was performed to analyze neoantigen *** Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in *** mutations were the most frequent types of somatic mutation in the coding sequence *** signature analysis detected signature 2.signature 6,and signature 7 in CSCC ***3CA,FBXW7,and BICRA were identified as potential driver genes,with BICRA as a newly reported *** variation profiling identified 4,960 potential neoantigens,of which 114 were listed in two neoantigen-related *** The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.

关键词： Cervical squamous cell carcinoma Genome alteration Neoantigens Immunotherapy

LC-MS/MS法测定人血浆中抗辐射新药E0703和代谢物E0703-tone的质量浓度及药动学研究

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沈阳药科大学学报 2018年第9期35卷 763-768页

作者：褚利娟甘慧高月窦桂芳孟志云邸欣沈阳药科大学药学院辽宁沈阳110016 中国人民解放军军事医学科学院野战输血研究所北京100850 中国人民解放军军事医学科学院放射与辐射医学研究所北京100850

目的建立同时测定人血浆中E0703及其代谢物E0703-tone的LC-MS/MS分析方法,并应用于E0703在人体内的药动学研究。方法以5-雄烯二醇为内标,血浆样品用乙腈沉淀蛋白后,以乙腈-体积分数0.1%甲酸水溶液(体积比85∶15)为流动相经CAPCELL PAK C... 详细信息

目的建立同时测定人血浆中E0703及其代谢物E0703-tone的LC-MS/MS分析方法,并应用于E0703在人体内的药动学研究。方法以5-雄烯二醇为内标,血浆样品用乙腈沉淀蛋白后,以乙腈-体积分数0.1%甲酸水溶液(体积比85∶15)为流动相经CAPCELL PAK C_(18)M GIII色谱柱分离,质谱检测采用电喷雾离子源(ESI)正离子化方式,以多反应监测(MRM)方式分别监测离子对m/z 339.2.2.3.1(E0703)、m/z 337.4→2.9.1(E0703-tone)和m/z 2.3.2.159.2.内标)。8名健康受试者单次口服E0703 2. mg,在不同时刻采集血浆样品进行LC-MS/MS分析,采用Phoenix 64(Win Nonlin6.4)的非房室模型计算药动学参数。结果 E0703和E0703-tone标准曲线的线性范围均为1~500μg·L^(-1)(r≥0.99),批内精密度分别≤8.0%和9.1%,批间精密度分别≤14.4%和13.3%;准确度分别为-10.0%~6.0%和-5.1%^-1.4%。E0703进入人体内迅速代谢为E0703-tone,E0703-tone的达峰时间为(1.2.±0.76)h,达峰质量浓度为(2..9±19.8)μg·L^(-1),血浆消除半衰期为(2.61±0.44)h。结论本方法适用于E0703人体药动学研究。

关键词：雌激素药药代动力学液相色谱-质谱法

A multiscale adaptive framework based on convolutional neural network: Application to fluid catalytic cracking product yield prediction

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Petroleum Science 2024年第4期21卷 2849-2869页

作者： Nan Liu Chun-meng Zhu meng-Xuan Zhang Xing-Ying Lan State Key Laboratory of Heavy Oil Processing China University of Petroleum (Beijing) College of Artificial Intelligence China University of Petroleum (Beijing)

Since chemical processes are highly non-linear and multiscale, it is vital to deeply mine the multiscale coupling relationships embedded in the massive process data for the prediction and anomaly tracing of crucial process parameters and production indicators. While the integrated method of adaptive signal decomposition combined with time series models could effectively predict process variables, it does have limitations in capturing the high-frequency detail of the operation state when applied to complex chemical processes. In light of this, a novel Multiscale Multi-radius Multi-step Convolutional Neural Network(Msrt Net) is proposed for mining spatiotemporal multiscale information. First, the industrial data from the Fluid Catalytic Cracking(FCC) process decomposition using Complete Ensemble Empirical Mode Decomposition with Adaptive Noise(CEEMDAN) extract the multi-energy scale information of the feature subset. Then, convolution kernels with varying stride and padding structures are established to decouple the long-period operation process information encapsulated within the multi-energy scale data. Finally, a reconciliation network is trained to reconstruct the multiscale prediction results and obtain the final output. Msrt Net is initially assessed for its capability to untangle the spatiotemporal multiscale relationships among variables in the Tennessee Eastman Process(TEP). Subsequently, the performance of Msrt Net is evaluated in predicting product yield for a 2.80 × 10～6 t/a FCC unit, taking diesel and gasoline yield as examples. In conclusion, Msrt Net can decouple and effectively extract spatiotemporal multiscale information from chemical process data and achieve a approximately reduction of 30% in prediction error compared to other time-series models. Furthermore, its robustness and transferability underscore its promising potential for broader applications.

关键词： Fluid catalytic cracking Product yield Data-driven modeling Multiscale prediction Data decomposition Convolution neural network