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Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

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Journal of Integrative Agriculture 2018年第10期17卷 2281-2291页

作者： LIU Wen-wen XIN Min CAO meng-ji qin meng LIU Hui ZHAO Shou-qi WANG Xi-feng State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China National Citrus Engineering Research Center Citrus Research Institute Southwest University Chongqing 400712 P.R. China National Agricultural Technology Extension and Service Center Beijing 100125 P.R. China

To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA（dsRNA） molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus（HaEV）. Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5′ untranslated region（UTR）, 3＇ UTR ending with the unique sequence CCCCCCCC and lacking a poly（A） tail. An open reading frame（ORF） that encodes a deduced 4 867 amino acids（aa） polyprotein with three domains： RdRP, Hel and UGT（UDP-glycosyltransferase）. HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization（FISH） experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.

关键词： common sunflower (Helianthus annuus) next-generation sequencing (NGS) double-stranded RNA Endornaviridae

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A Tri-mode of Mock-Walker Cells

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Advances in Atmospheric Sciences 2024年第4期41卷 671-679页

作者： Han qin Ji NIE Zhiyong meng Laboratory for Climate and Ocean-Atmosphere Studies Department of Atmospheric and Oceanic SciencesSchool of PhysicsPeking UniversityBeijing 100871China China Meteorological Administration Tornado Key Laboratory Foshan 528061China

This work uses cloud-resolving simulations to study mock-Walker cells driven by a specified sea surface temperature(SST).The associated precipitation in the mock-Walker cells exhibits three different modes,including a single peak of precipitation over the SST maximum(mode 1),symmetric double peaks of precipitation straddling the SST maximum(mode 2),and a single peak of precipitation on one side of the SST maximum(mode 3).The three modes are caused by three distinct convective activity center migration *** indicate that the virtual effect of water vapor plays an important role in differentiating the three *** the SST gradient is large,the virtual effect may be strong enough to overcome the temperature effect,generating a low-level low-pressure anomaly below the ascending branch of the Walker cell off the *** results here highlight the importance of the virtual effect of water vapor and its interaction with convection and large-scale circulation in the Walker circulation.

关键词： Walker circulation cloud-resolving simulation virtual effect

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Reducing CO_(2)emissions from the rebalancing operation of the bike-sharing system in Beijing

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Frontiers of Engineering Management 2023年第2期10卷 262-284页

作者： meng qin Jiayu WANG Wei-Ming CHEN Ke WANG Center for Energy and Environmental Policy Research Beijing Institute of TechnologyBeijing 100081China School of Management and Economics Beijing Institute of TechnologyBeijing 100081China Sustainable Development Research Institute for Economy and Society of Beijing Beijing 100081China Beijing Key Lab of Energy Economics and Environmental Management Beijing 100081China

With the development of the bike-sharing system(BSS)and the introduction of green and low carbon development,the environmental impacts of BSS had received increasing attention in recent ***,the emissions from the rebalancing of BSS,where fossil-fueled vehicles are commonly used,are usually neglected,which goes against the idea of green travel in a sharing *** studies on the bike-sharing rebalancing problem(BRP),which is considered NP-hard,have mainly focused on algorithm innovation instead of improving the solution model,thereby hindering the application of many existing models in large-scale *** study then proposes a method for optimizing the CO_(2)emissions from BRP and takes the BSS of Beijing as a *** initially analyze the spatial and temporal characteristics of BSS,especially the flow between districts,and find that each district can be independently ***,we develop a rebalancing optimization model based on a partitioning strategy to avoid deciding the number of bikes being loaded or unloaded at each parking *** then employ the tabu search algorithm to solve the *** show that(i)due to over launch and lack of planning in rebalancing,the BSS in Beijing shows great potential for optimization,such as by reducing the number of vehicle routes,CO_(2)emissions,and unmet demands;(ii)the CO_(2)emissions of BSS in Beijing can be reduced by 57.5%by forming balanced parking nodes at the end of the day and decreasing the repetition of vehicle routes and the loads of vehicles;and(iii)the launch amounts of bikes in specific districts,such as Shijingshan and Mentougou,should be increased.

关键词： bike-sharing CO_(2)emissions environmental benefit partitioning strategy rebalancing problem

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系统运动训练对青少年人格发展的影响:一项来自射击项目的3年纵向跟踪研究

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体育科研 2024年第5期45卷 72-78页

作者：黄志剑何施孟勤童欣郑轩湖北大学体育学院湖北武汉430062 湖北商贸学院湖北武汉430079 湖北省射击运动管理中心湖北武汉430205

目的:考察3年时间前后长期系统参与射击项目训练比赛的青少年人格发展变化情况,以探讨内外部因素对人格发展的影响。方法:采用NEO-FFI五因素人格问卷中文版于2019年8月对120名参加省级射击比赛的青少年运动员进行测试,并于2022年进行第... 详细信息

目的:考察3年时间前后长期系统参与射击项目训练比赛的青少年人格发展变化情况,以探讨内外部因素对人格发展的影响。方法:采用NEO-FFI五因素人格问卷中文版于2019年8月对120名参加省级射击比赛的青少年运动员进行测试,并于2022年进行第二次测试,共获得46份有效样本,参与者总体保留率为38.3%。对2次调查的数据进行以重复测量方差分析为主的统计分析。结果:时间(前/后)×射击训练(是/否)的重复测量方差分析结果显示,时间因素在外向性、开放性和尽责性等人格因素得分上的变化主效应显著;射击训练因素主效应不显著;时间和射击训练的交互作用在尽责性因素得分上显著。结论:①与系统射击训练相比,时间所代表的主体内部(遗传)因素对人格发展变化的影响更为显著。②坚持从事射击训练的青少年与未坚持训练的同龄人相比,在3年间的人格变化幅度更小,说明系统的运动训练对于人格发展与变化而言是一种稳定性因素。今后研究应增加样本量和时间跨度,并将更多不同类型的运动项目纳入考察的范围。

关键词：人格系统运动训练射击纵向研究青少年

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基于STM32单片机的太阳能自动跟踪锂电池充电系统设计

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现代信息科技 2024年第9期8卷 34-38,42页

作者：秦蒙范锐赵娟郎芮王艺龙重庆电力高等专科学校信息工程学院重庆400053

随着经济全球化和世界经济的迅猛发展,人们对煤炭、石油等传统能源的需求越来越高,但该类资源不仅储藏有限,而且容易污染环境。与此同时,以太阳能为代表的新能源逐渐进入人们的视野。在此背景下,为进一步提高太阳能的利用率,文章提出一... 详细信息

随着经济全球化和世界经济的迅猛发展,人们对煤炭、石油等传统能源的需求越来越高,但该类资源不仅储藏有限,而且容易污染环境。与此同时,以太阳能为代表的新能源逐渐进入人们的视野。在此背景下,为进一步提高太阳能的利用率,文章提出一种基于STM32单片机的太阳能自动跟踪锂电池充电系统的设计方案,利用光照传感器采集四个方向的光照强度将其转换为电信号,通过AD转换将电信号转化为数字信号,再由STM32单片机进行数据处理分析,并根据四个方向的光照强度驱动电机旋转,使太阳能电池板与太阳光尽量保持垂直,最大限度提高太阳能的吸收效率。

关键词： STM32单片机自动跟踪锂电池充电

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Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1M

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第9期8卷 1514-1520页

作者： ZHU Yong GAN Jun-jiang ZHANG Guo-liang YAO Bin ZHU Wen-jie meng qin Shool of Materials Science and Chemical Engineering Zhejiang University Hangzhou 310027 China School of Biological and Chemical Engineering Zhejiang Uiversity of Science and Technology Hangzhou 310032 China Institute of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310014 China

In this work,rhamnolipid production was investigated using waste frying oil as the sole carbon source. By culture in shaking flasks,a naturally isolated strain synthesized rhamnolipid at concentration of 12.47 g/L and its mutant after treatment by UV light increased this productivity to 24.61 g/L. Fermentation was also conducted in a 50 L bioreactor and the productivity reached over 20 g/L. Hence,with a stable and high productive mutant strain,it could be feasible to reuse waste frying oil for rhamnolipid production on industrial scale.

关键词： Rhamnolipid Pseudomonas aeruginosa Biosurfactant Waste frying oil

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Effects of Labeling Thiophilic FRET Dyes on the Stability and Dimerization Process of β-Lactoglobulin

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Chinese Physics Letters 2011年第11期28卷 271-274页

作者： PAN Hai XIE Jin-Bing CAO Yi qin meng WANG Wei National Laboratory of Solid State Microstructure and Department of Physics Nanjing UniversityNanjing 210093

The stability and dimeric state of𝛽β-lactoglobulin(β-lg)can be dramatically affected by labeling the thiophilic agent to Cys121,whereas the underlining mechanism of such an effect is still *** label a fluorescenceresonance-energy-βtransfer(FRET)pair of donor(1,5-IAEDANS)and acceptor(5-IAF)dyes to Cys121 of𝛽β-lg monomers to investigate the effect of bulky thiophilic modification on the structure and stability of𝛽β-*** is found that the modification dramatically destroys the native structure ofβ-lg and results in an obvious increase of the𝛼-helical content,coincident with the accumulation of non-native𝛼α-helical intermediates during its folding ***,the dimeric state of𝛽β-lg can still be reached whereas its dimerization rate decreases dramatically,allowing us to characterize the dimerization process using the FRET method based on a stoppedflow *** results reveal that the dimerization process occurs before the completely folding of individual monomers,providing direct evidence on the cooperativity of folding and binding processes.

关键词： process helical stability

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L_(1)-Smooth SVM with Distributed Adaptive Proximal Stochastic Gradient Descent with Momentum for Fast Brain Tumor Detection

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Computers, Materials & Continua 2024年第5期79卷 1975-1994页

作者： Chuandong qin Yu Cao Liqun meng School of Mathematics and Information Science North Minzu UniversityYinchuan750021China Ningxia Key Laboratory of Intelligent Information and Big Data Processing Yinchuan750021China

Brain tumors come in various types,each with distinct characteristics and treatment approaches,making manual detection a time-consuming and potentially ambiguous *** tumor detection is a valuable tool for gaining a deeper understanding of tumors and improving treatment *** learning models have become key players in automating brain tumor *** descent methods are the mainstream algorithms for solving machine learning *** this paper,we propose a novel distributed proximal stochastic gradient descent approach to solve the L_(1)-Smooth Support Vector Machine(SVM)classifier for brain tumor ***,the smooth hinge loss is introduced to be used as the loss function of *** avoids the issue of nondifferentiability at the zero point encountered by the traditional hinge loss function during gradient descent ***,the L_(1) regularization method is employed to sparsify features and enhance the robustness of the ***,adaptive proximal stochastic gradient descent(PGD)with momentum,and distributed adaptive PGDwithmomentum(DPGD)are proposed and applied to the L_(1)-Smooth *** computing is crucial in large-scale data analysis,with its value manifested in extending algorithms to distributed clusters,thus enabling more efficient processing ofmassive amounts of *** DPGD algorithm leverages Spark,enabling full utilization of the computer’s multi-core *** to its sparsity induced by L_(1) regularization on parameters,it exhibits significantly accelerated convergence *** the perspective of loss reduction,DPGD converges faster than *** experimental results show that adaptive PGD withmomentumand its variants have achieved cutting-edge accuracy and efficiency in brain tumor ***-trained models,both the PGD andDPGD outperform other models,boasting an accuracy of 95.21%.

关键词： Support vector machine proximal stochastic gradient descent brain tumor detection distributed computing

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Strong and Injectable Hydrogels Based on Multivalent Metal Ion-Peptide Cross-linking

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Chemical Research in Chinese Universities 2020年第5期36卷 962-969页

作者： YU Wenting XUE Bin ZHU Zhenshu SHEN Ziqin qin meng WANG Wei CAO Yi Collaborative Innovation Center of Advanced Microstructures National Laboratory of Solid State MicrostnictureDepartment of PhysicsNanjing UniversityNanjing 210093P.R.China Shenzhen Research Institute of Nanjing University Shenzhen 518057P.R.China International Center Nanjing Foreign Language SchoolNanjing210000P.R.China

Injectable hydrogels are ideal biomaterials tor delivering cells,growth tactors and dmgs specifically to localized lesions and subsequent controlled *** factors can a fleet the efficacy of injectable *** avoid potential damage to encapsulated cells or dmgs,injectable hydrogels should be highly dynamic so that they can undergo shear-thinning at low strain rates and rapidly refonn after ***,dynamic hydrogels are often mechanically weak,leading to the leakage of encapsulated cells or *** we demonstrated a convenient method to improve the mechanical strength without jeopardizing the dynamic properties of hydrogels by using metal ion-peptide crosslinkers containing multiple metal ion-ligand *** showed that the dynamic properties of the hydrogels correlated with the intrinsic dynamics of the metal-ligand bonds and were not atlected by the fonnation of multivalent ***,the mechanical stability of the hydrogels was significantly improved due to the increased themiodynamic stability of the *** turthcr showed that the drug release rates were slowed down by the formation of multivalent *** results highlight the importance of ligand valency to themechanical response of hydrogels and provide a universal route to rationally tune the dynamic and mechanical properties of injectable hydrogels.

关键词： Peptide-ion coordination Ligand cooperativity Tnjectable hydrogek Mechanical response

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从“胆”探讨脑小血管病病机

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中医康复 2024年第5期1卷 43-46页

作者：李梦琴蔡昭莲刘光辉福建中医药大学福建福州350122 福建中医药大学附属人民医院福建福州350004

基于脑小血管病的影像学表现及临床证候等特点分析,本文依据“病厥阴风木者,肝将取决于胆”“胆主决断”“情志各异,必资胆气”“髓之虚实,肝胆应之”等古籍论述,结合当代各医家学者关于脑小血管病的辨证论治认识,进行了“胆”与脑小血... 详细信息

基于脑小血管病的影像学表现及临床证候等特点分析,本文依据“病厥阴风木者,肝将取决于胆”“胆主决断”“情志各异,必资胆气”“髓之虚实,肝胆应之”等古籍论述,结合当代各医家学者关于脑小血管病的辨证论治认识,进行了“胆”与脑小血管病病机相关性的初步理论探讨。笔者以为,围绕“胆木风气”“胆主决断”及“五脏情志成用”三方面的病理、生理特性阐述,能够为脑小血管病病机提供新的理论依据,为脑小血管病临床治疗提供从胆论治之新思路。

关键词：脑小血管病执行功能胆主决断胆木风气神志

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