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Investigation of Biobutanol Efficiency of Chlorella sp. Cultivated in Municipal Wastewater

Journal of Geoscience and Environment Protection 2018年第10期6卷 40-50页

作者： melih onay Computational & Experimental Biochemistry Lab. Department of Environmental Engineering Van Yuzuncu Yil University Van Turkey

Many strains of microalgae can grow in wastewaters through their ability to utilize inorganic nitrogen and phosphorus in wastewater. The content of municipal wastewater changes from a location to others. Biofuel production from municipal wastewater has gained huge importance due to progresses in cultivation of microalgae in wastewaters. Biobutanol is produced by the acetone-butanol-ethanol (ABE) fermentation. In this study, we examined the biobutanol production efficiency of Chlorella sp. DEE006 which is cultivated in the municipal wastewater in flat-photobioreactor. Growth of microalgae was monitored at 680 nm using spectrophotometer and the biomass was also pre-treated with acidic hydrolysis (1 M H2SO4). Total carbohydrate and protein contents were measured. Fermented microalgae samples were taken for calculation of biobutanol concentration. We obtained both high biobutanol content (6.23 ± 0.19 g·L−1) and high bioethanol yield 0.16 ± 0.005 g (g sugar)−1. 50% wastewater had the highest biomass concentration (1930 ± 11 mg/L) among the wastewaters with five various concentrations. It had the highest biomass productivity with 0.28 ± 0.001 g L−1d−1. Also, it obtained the highest carbohydrate and protein concentration with 0.80 ± 0.02 gL−1 and 0.95 ± 0.01 gL−1, respectively. According to our results, Chlorella sp. DEE006 can be used for large scale biobutanol production in the future.

关键词： Microalgae Chlorella Biobutanol Biofuel Municipal Wastewater

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Evaluation of Various Extraction Techniques for Efficient Lipid Recovery from Thermo-Resistant Microalgae, Hindakia, Scenedesmus and Micractinium Species—Comparison of Lipid Extraction Methods from Microalgae

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American Journal of Analytical Chemistry 2016年第2期7卷 141-150页

作者： melih onay Cagla Sonmez Huseyin A. Oktem Meral Yucel Department of Chemistry Yuzuncu Yil University Van Turkey Department of Biology Middle East Technical University Ankara Turkey Department of Biotechnology Middle East Technical University Ankara Turkey

In recent years, photosynthetic microalgae regained attention for biodiesel production. For efficient utilization of microalgae, a number of criteria including a strain with high biomass and lipid productivities and employment of effective and reliable methods for oil extraction from the obtained biomass should be met. Recently, we have isolated and identified three thermo-resistant green microalgae strains, namely;Scenedesmus sp. ME02, Hindakia tetrachotoma ME03 and Mic-ractinium sp. ME05. In this study, we compared percent lipid content of thermos-tolerant mic-roalgal strains using the following solvent extraction methods: Soxhlet, Bligh and Dyer and Folch methods with or without assisted cell disruption techniques including lyophilization, homogenization, ultrasonication, bead and microwave-assisted. The highest increase in lipid yield was obtained with a combination of lyophilization and ultrasonication techniques together with Soxhlet method: 27% of total dry weight for Micractinium sp. ME05. We conclude that lyophilization and ultrasonication are effective assistance methods for lipid extraction from thermo-resistant microalgae.

关键词： Lipid Extraction Thermo-Resistant Green Microalgae Cell Disruption Techniques Ultrasonication Biodiesel

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