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Characterization of hepatic progenitors from human fetal liver during second trimester

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World Journal of Gastroenterology 2008年第37期14卷 5730-5737页

作者： mekala subba rao Aleem Ahmed Khan Nyamath Parveen Mohammed Aejaz Habeeb Chittoor Mohammed Habibullah Gopal Pande Centre for Cellular and Molecular Biology Uppal Road Hyderabad 500007 India Centre for Liver Research and Diagnostics Kanchanbagh Hyderabad 500058 India

AIM： To enrich hepatic progenitors using epithelial cell adhesion molecule （EpCAM） as a marker from human fetal liver and investigate the expression of human leukocyte antigen （HLA） and their markers associated with hepatic progenitor cells. METHODS： EpCAM ＋ve cells were isolated using magnetic cell sorting （MACS） from human fetuses （n = 10） at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein （AFP）, CD29 （integrin ~1）, CD49f （integrin c^6） and CD90 （Thy 1） was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class Ⅰ （A, B, C） and class Ⅱ （DR） expression was studied by flow cytometry only. RESULTS： FACS analysis indicated that EpCAM ＋ve cells were positive for CD29, CD49f, CD90, CD34, HLA class I, albumin and AFP but negative for HLA class Ⅱ （DR） and CD45. RT PCR showed that EpCAM ＋ve cells expressed liver epithelial markers （CK18）, biliary specific marker （CK19） and hepatic markers （albumin, AFP）. On immunocytochemical staining, EpCAM ＋ve cells were shown positive signals for CK18 and albumin. CONCLUSION： Our study suggests that these EpCAM ＋ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor.

关键词： Epithelial cell adhesion molecule Human fetal liver Hepatic progenitors Human leukocyte antigen Hepatic cell therapy

Identification of circulating CD90^+ CD73^+ cells in cirrhosis of liver

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World Journal of Stem Cells 2011年第7期3卷 63-69页

作者： Mitnala Sasikala Pugazhelthi Surya Gaddipati Radhika Pondugala Pavan Kumar mekala subba rao Rathindra Mohan Mukherjee Padaki Nagaraja rao D Nageshwar Reddy Institute of Basic Sciences and TranslationalResearch Asian Health Care Foundation Asian Institute of Gastroenterology

AIM:To identify circulating CD90 + CD73 + CD45 cells and evaluate their in vitro proliferating ***:Patients with cirrhosis(n=43),and healthy volunteers(n=40)were recruited to the *** cells were isolated and cultured from the peripheral blood of controls and cirrhosis ***-like cells that appeared in cultures were analyzed for morphological features,enumerated by flow cytometry and confirmed by immunocytochemistry(ICC).Colony forming efficiency(CFE)of these cells was assessed and expressed as a ***:In comparison to healthy volunteers,cells obtained from cirrhotic patients showed a significantincrease(P<0.001)in the percentage of CD90+CD73+ CD45 cells in *** cells also showed 10 fold increases in *** cytometry and ICC confirmed that the proliferating cells expressed CD90 + CD73 + in the cultures from cirrhosis ***:These results indicate the presence of circulating CD90 + CD73 + CD45 cells in patients with liver cirrhosis that have the potential to proliferate at a higher rate.

关键词： CD90+ CD73+ CD45 cells Liver cirrhosis Proliferation Colony forming efficiency

A novel approach for speaker diarization system using TMFCC parameterization and Lion optimization

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Journal of Central South University 2017年第11期24卷 2649-2663页

作者： V.subba Ramaiah R.Rajeswara rao Mahatma Gandhi Institute of Technology KokapetHyderabadTelangana 500075India Jawaharlal Nehru Technological University Kakinada KakinadaAndhra Pradesh 535002India

In audio stream containing multiple speakers, speaker diarization aids in ascertaining "who speak when". This is an unsupervised task as there is no prior information about the speakers. It labels the speech signal conforming to the identity of the speaker, namely, input audio stream is partitioned into homogeneous segments. In this work, we present a novel speaker diarization system using the Tangent weighted Mel frequency cepstral coefficient(TMFCC) as the feature parameter and Lion algorithm for the clustering of the voice activity detected audio streams into particular speaker groups. Thus the two main tasks of the speaker indexing, i.e., speaker segmentation and speaker clustering, are improved. The TMFCC makes use of the low energy frame as well as the high energy frame with more effect, improving the performance of the proposed system. The experiments using the audio signal from the ELSDSR corpus datasets having three speakers, four speakers and five speakers are analyzed for the proposed system. The evaluation of the proposed speaker diarization system based on the tracking distance, tracking time as the evaluation metrics is done and the experimental results show that the speaker diarization system with the TMFCC parameterization and Lion based clustering is found to be superior over existing diarization systems with 95% tracking accuracy.

关键词： speaker diarization Mel frequency cepstral coefficient i-vector extraction Lion algorithm

Effectiveness of sodium silicate as gangue depressants in iron ore slimes flotation

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International Journal of Minerals,Metallurgy and Materials 2011年第5期18卷 515-522页

作者： Danda Srinivas rao Tadiparthi Venkata VijayaKumar Sripada subba rao Swarna Prabhakar Guntamadugu Bhaskar Raju National Metallurgical Laboratory-Madras Centre Council of Scientific and Industrial Research-Madras ComplexChennai 600 113India

The recovery of iron from the screw classifier overflow slimes by direct flotation was studied. The relative effectiveness of sodium silicates with different silica-to-soda mole ratios as depressants for silica and silicate bearing minerals was investigated. Silica-to-soda mole ratio and silicate dosage were found to have significant effect on the separation efficiency. The results show that an increase of Fe content in the concentrate is observed with concomitant reduction in SiO2 and A1203 levels when a particular type of sodium silicate at a proper dosage is used. The concentrate of 58.89wt% Fe, 4.68wt% SiO2, and 5.28wt% A1203 with the weight recovery of 38.74% and the metal recovery of 41.13% can be obtained from the iron ore slimes with 54.44wt% Fe, 6.72wt% SiO2, and 6.80wt% A1203, when NazSiO3 with a silica-to-soda mole ratio of 2.19 is used as a depressant at a feed rate of 0.2 kg/t.

关键词： iron ores sodium silicate depressants flotation

Implications for the lithospheric structure of Cambay rift zone,western India:Inferences from a magnetotelluric study

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Geoscience Frontiers 2020年第5期11卷 1743-1754页

作者： Nagarjuna Danda C.K.rao Amit Kumar P.Rama rao P.B.V.subba rao Dr.K.S.Krishnan Geomagnetic Research Laboratory IIGAllahabad221505India Indian Institute of Geomagnetism New PanvelNavi Mumbai410218India Department of Geophysics Andhra UniversityVisakhapatnam530003India

Broad-band and long-period magnetotelluric(MT)data were acquired along an east-west trending traverse of nearly 200 km across the Kachchh,Cambay rift basins,and Aravalli-Delhi fold belt(ADFB),western *** regional strike analysis of MT data indicated an approximate N59°E geoelectric strike direction under the traverse and it is in fair agreement with the predominant geological strike in the study *** decomposed transverse electric(TE)-and transverse magnetic(TM)-data modes were inverted using a nonlinear conjugate gradient algorithm to image the electrical lithospheric structure across the Cambay rift basin and its surrounding *** studies show a thick(~1-5 km)layer of conductive Tertiary-Mesozoic sediments beneath the Kachchh and Cambay rift *** resistive blocks indicate presence of basic/ultrabasic volcanic intrusives,depleted mantle lithosphere,and different Precambrian structural *** crustal conductor delineated within the ADFB indicates the presence of fluids within the fault zones,sulfide mineralization within polyphase metamorphic rocks,and/or Aravalli-Delhi sediments/*** observed conductive anomalies beneath the Cambay rift basin indicate the presence of basaltic underplating,volatile(CO2,H2 O)enriched melts and channelization of melt fractions/fluids into crustal depths that occurred due to plume-lithosphere *** variations in electrical resistivity observed across the profile indicate that the impact of Reunion plume on lithospheric structures of the Cambay rift basin is more dominant at western continental margin of India(WCMI)and thus support the hypothesis proposed by Campbell&Griffiths about the plume-lithosphere interactions.

关键词： Cambay rift Deccan basalts Magnetotellurics Partial melt Reunion plume

CeCl_3·7H_2O-SiO_2:Catalyst promoted microwave assisted neat synthesis,antifungal and antioxidant activities of α-diaminophosphonates

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Chinese Chemical Letters 2013年第8期24卷 759-763页

作者： subba rao Devineni Srinivasulu Doddaga Rajasekhar Donka Naga Raju Chamarthi Department of Chemistry Sri Venkateswara University

CeCl_3·7H_2O supported on silica（CeCl_3·7H_2O-SiO_2） is used as a heterogeneous,efficient and recyclable catalyst for a three component one-pot reaction of an amine,aldehydes and diethyl phosphite to synthesizeα-diaminophosphonate derivatives under microwave irradiation exploiting neat reaction ***α-diaminophosphonates（6a-j） of 4,4＇-sulfonyldianiline（Dapsone）（3） were synthesized and structural elucidation was confirmed by spectral *** and antioxidant activities were evaluated include minimum inhibitory concentrations and IC_（50） values,respectively of the titled *** 6h,6i exhibited promising antioxidant activity at lower IC_（50） values 53.7μg/mL, 53.2μg/mL,respectively as compared with standard IC_（50） value 51.6μg/mL.

关键词： α-Diaminophosphonates Green synthesis Kabachnik-Fields reaction Antifungal activity Antioxidant activity

PGE and isotopic characteristics of Shergol and Suru Valley Ophiolites,Western Ladakh:Implications for supra-subduction tectonics along Indus Suture Zone

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Geoscience Frontiers 2021年第3期12卷 473-488页

作者： Irfan Maqbool Bhat Talat Ahmad D.V.subba rao Srinivasan Balakrishnan N.V.Chalapathi rao Department of Earth Sciences University of KashmirSrinagar 190006India Vice Chancellors Office University of KashmirSrinagar 190006India Geochemistry Division CSIR-National Geophysical Research Institute(NGRI)Hyderabad 500007India Department of Earth Sciences Pondicherry UniversityPuducherry 605014India Centre of Advanced Study in Geology Institute of ScienceBanaras Hindu UniversityVaranasi 221005India

Present study reports the PGE-geochemistry of mantle peridotites and Nd-isotope geochemistry of arc related mafic rocks from the Indus Suture Zone(ISZ),western *** total PGE concentration of the Shergol and Suru Valley peridotites(∑PGE=96-180 ppb)is much higher than that of the primitive mantle and global ophiolitic mantle *** studied peridotites show concave upward PGE-distribution patterns with higher palladium-group PGE/Iridium-group PGE ratios(i.e.,0.8-2.9)suggesting that the partial melting is not the sole factor responsible for the evolution of these *** observed PGE-distribution patterns are distinct from residual/refractory mantle peridotites,which have concave downward or flat PGE-distribution *** enrichment of palladium-group PGE as well as other whole-rock incompatible elements(e.g.,LILE and LREE)and higher Pd/Ir ratio(1.1-5.9)reflects that these peridotites have experienced fluid/melt interaction in a supra-subduction zone(SSZ)tectonic ***,the Shergol mafic intrusives and Dras mafic volcanics,associated with the studied peridotites,have high^(143)Nd/^(144)Nd ratios(i.e.,0.512908-0.513078 and 0.512901-0.512977,respectively)and positiveε_(Nd)(t)(calculated for t=140 Ma)values(i.e.,+5.3 to+8.6 and+5.1 to+6.6,respectively),indicating derivation from depleted mantle sources within an intra-oceanic arc setting,similar to Spongtang and Nidar ophiolites from other parts of Ladakh *** transition from SSZ-type Shergol and Suru Valley peridotites to Early Cretaceous tholeiitic Shergol mafic intrusives followed by tholeiitic to calc-alkaline Dras mafic volcanics within the Neo-Tethys Ocean exhibit characteristics of subduction initiation mechanism analogous to the Izu-Bonin-Mariana arc system within western Pacific.

关键词： PGE Nd-isotope Mantle peridotites Ophiolites Ladakh Neo-Tethys Ocean

Beneficiation of a low grade limestone sample

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Mining Science and Technology 2011年第5期21卷 631-636页

作者： rao Danda Srinivas Vijayakumar Tadiparthi Venkata subba rao Sripada Bhaskar Raju Guntamadugu Prabhakar Swarna National Metallurgical Laboratory Madras Centre CSIR Madras Complex Chennai 600 113 India

Pilot scale column flotation studies were conducted on a low grade siliceous limestone ore. Silica content was reduced to less than 13g in the concentrate so that it became satisfactory for use in the paper or rubber industries. The limestone sample was crystalline and constituted primarily of calcite that contained quartz, feldspar, pyroxene, and biotite as gangue minerals. Quartz is the major silicate gangue whereas feldspar, pyroxene, and biotite exist in minor to trace quantities. Traces of pyrite were also observed within the sample. A reverse flotation process was adopted where the silicate gangue minerals were floated using two different commercial cationic collectors： Chem-750 F or Floatamine-D. The studies clearly suggest it is possible to produce a limestone concentrate assaying around 96-97% CaCO3 containing less than 1 % Si02. The effect of feed flow rate, percent solids, froth depth, and wash water on the grade and recovery of the CaC03 concentrate is discussed.

关键词： Low grade limestoneReverse flotationColumn flotationCationic collector

Synthesis,isolation,identification and characterization of new process-related impurity in isoproterenol hydrochloride by HPLC,LC/ESI-MS and NMR

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Journal of Pharmaceutical Analysis 2017年第6期7卷 394-400页

作者： Neeraj Kumar subba rao Devineni Prasad Reddy Gajjala Shailendra Kumar Dubey Pramod Kumar Chemical Research Department Micro Labs LtdAPI DivisionBommasandra-Jigani Link RoadKIADB INDL AreaBommasandraBangalore 560105KarnatakaIndia

One unknown impurity （Imp-II） during the analysis of laboratory batches of isoproterenol hydrochloride was detected in the level ranging from 0.04% to 0.12% by high performance liquid chromatography with UV detection. The unknown impurity structure was proposed as 4-[2-（propan-2oylamino）ethyl]benzene-l,2-diol （Imp-II） using the liquid chromatography-mass spectrophotometry （LC-MS） analysis. Imp-II was isolated by semi-preparative liquid chromatography from the impurity-enriched reaction crude sample. Its proposed structure was confirmed by nuclear magnetic spectroscopy such as 1H, 13C, DEPT （1D NMR）, HSQC （2D NMR） and infrared spectroscopy （IR）, and retention time and purity with HPLC followed by the chemical synthesis. Due to less removable nature of Imp-II during the purification, the synthetic process was optimized proficiently to control the formation of Imp-II below to the limit 〈 0.12% in the course of reaction. The new chemical route was developed for the preparation of this impurity in required quantity with purity to use as reference standard. The most probable mechanism for the formation of Imp-II was discussed in details.

关键词： Isoproterenol hydrochloride Impurities HPLC NMR