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Determination of Total Anthraquinone and Free Anthraquinone in Different Processed Products of Rhubarb

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Medicinal Plant 2021年第2期12卷 17-20页

作者： Bing WU Jinhua LIU meiyuan lu Chao ZENG Tingting MO Liuzhou Hospital of Traditional Chinese Medicine Liuzhou 545001 China The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning 530023 China Guangxi Food and Drug Evaluation & Inspection Center Nanning 530029 China

[Objectives]The content of total anthraquinone and 5 free anthraquinones in different processed products of rhubarb were determined.[Methods]Using emodin as the reference,the concentration of 1%magnesium acetate methanol solution was used for color development,and the absorbance was measured at 516 nm wavelength;the mobile phase was methanol-0.1%phosphoric acid(76∶24)aqueous solution,the detection wavelength was set at 256 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the sample size was 10μL.[Results]The content of total anthraquinones in rhubarb was determined by UV *** linear range was 0.98-23.52μg/mL,R2=0.9993,the average recoveries were 100.12%,99.66%and 99.15%,with RSDs of 1.58%,0.94%and 1.41%,*** linear ranges of emodin,emodin,emodin,chrysophanol and emodin methyl ether were 0.0207-0.414(R2=0.9999),0.1721-3.442(R2=0.9996),0.0203-0.406(R2=0.9999),0.148-2.96(R2=0.9998)and 0.164-3.28(R2=0.9993),respectively.[Conclusions]The contents of total anthraquinones and free anthraquinones in different processed products of rhubarb were determined by UV spectrophotometry and *** method can be used for quality control of rhubarb and its different processed products.

关键词： Rheum L. Total anthraquinone Free anthraquinones High-performance liquid chromatography(HPLC) Ultraviolet-visible spectrophotometry(UV-Vis)

Study on the Effect of Aqueous Extract of Houttuynia cordata on Serum TNF-αand IL-6 Content in Mice Infected with Five Common Pathogenic Bacteria

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Medicinal Plant 2020年第5期11卷 68-70,75页

作者： meiyuan lu Chunli TANG Fengxian ZHAO Xiaoyu HUANG Hongxia CHEN Meifang LI Guangxi Food and Drug Evaluation and Inspection Center Nanning 530029China The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning 530023China Guangxi University of Chinese Medicine Nanning 530201China

[Objectives]To observe the effect of aqueous extract of Houttuynia cordata on the content of serum TNF-αand IL-6 in mice with abdominal infection caused by Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Proteus vulgaris and Klebsiella pneumoniae,so as to investigate the antibacterial effect of aqueous extract of Houttuynia cordata in vivo.[Methods]A total of 72 KM mice were randomly divided into six groups:model group,blank group,cefdinir dispersible tablet group(0.25 g/kg),high,medium and low dose groups of aqueous extract of Houttuynia cordata(40,20,10 g/kg crude drug).There were 12 mice in each group,half male and half female,continuously given intragastric administration for three days,once a *** blank group and the model group were intragastrically infused with normal saline,and the other groups were given corresponding drugs with a volume of 0.02 mL/*** 30 min of administration on the third day,the mice were injected intraperitoneally with 0.5 mL of bacterial suspension to make bacterial *** mice in the blank group were intraperitoneally injected with the same amount of normal saline,followed by intragastric administration 6 h after intraperitoneal injection and intragastric administration again after 24 *** mice were observed for three days,and the changes of body weight before and after infection were *** the fourth day of infection,eyeball blood was taken,serum was separated,and the concentration of inflammatory factors TNF-αand IL-6 in serum was determined.[Results]Compared with the model group,the high,medium and low dose groups of *** aqueous extract had protective effects on mice with abdominal infection caused by ***,***,***,*** and ***,and decreased the content of TNF-αand IL-6 in serum.[Conclusions]The aqueous extract of *** had a certain antibacterial effect on acute abdominal infection caused by ***,***,***,*** and ***,and its me

关键词： Aqueous extract of Houttuynia cordata TNF-α IL-6 Antibacterial activity in vivo

Determination of Polydatin in Rat Serum and Its Changes by UPLC Method

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Medicinal Plant 2021年第2期12卷 63-64,69页

作者： Haisheng ZENG Zhiqiang WANG meiyuan lu Chao ZENG Mengxia YANG Guilin YANG The Affiliated Hospital of Youjiang Medical University for Nationalities Baise 533000 China The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning 530023 China Guangxi Food and Drug Evaluation & Inspection Center Nanning 530029 China

[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the analytical column,acetonitrile-water(55∶45)was used as the mobile phase,the flow rate was 0.5 mL/min,and the column temperature was 30℃and the detection wavelength was 306 nm.[Results]The linear range of the established serum sample analysis method was 1.0-20.0μg/mL,and the correlation coefficient was r=0.9994;the intraday and interday RSD of Wistar rat serum was less than 3.0%,and the accuracy was higher than 90%.[Conclusions]This method is sensitive,accurate,and *** is suitable for monitoring the concentration of polydatin in serum after intragastric administration,and can also be used for pharmacokinetics and bioavailability studies.

关键词： Polydatin Drug concentration in serum Content determination Ultra Performance Liquid Chromatography(UPLC)

Optimization of the Extraction Process of Rutin from Flos Sophorae by Orthogonal Experiments

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Agricultural Biotechnology 2021年第4期10卷 120-122,126页

作者： Shan LI meiyuan lu Lin YAO Xinying MO Guilin YANG Haisheng ZENG Guangxi Food and Drug Evaluation&Inspection Center Nanning 530029China Youjiang Medical University for Nationalities Baise 533000China The Affiliated Hospital of Youjiang Medical University for Nationalities Baise 533000China

[Objectives]This study was conducted to optimize the extraction process of rutin in Flos Sophorae.[Methods]With the percentage content of rutin extracted from Flos Sophorae as the evaluation index and rutin as the reference substance,the content of rutin in Flos Sophorae extracted ultrasonically was determined by high performance liquid chromatography.L_(9)(3^(4))orthogonal experiments were carried out to optimize the three factors(solvent concentration,material-to-liquid ratio,extraction time)that affect the effect of ultrasonic extraction of rutin from Flos Sophorae.[Results]The extraction effect of the crude medicinal powder was best with the methanol concentration of 80%,the ratio of material to liquid at 0.1∶30,and the extraction time of 40 min.[Conclusions]This study provides a reference for optimizing the extraction process of rutin in Flos Sophorae.

关键词： Flos Sophorae Rutin Extraction process High performance liquid chromatography

Research Advances in Functional Constituents of Chickpea

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Asian Agricultural Research 2019年第4期11卷 59-61页

作者： Haitian YU Aiqing ZHENG meiyuan lu Feng YANG Chaoqin HU Xin YANG Yuhua HE Liping WANG Institute of Food Crops Yunnan Academy of Agricultural Sciences

Based on the generalization and summary of the research on the functional constituents of chickpea at home and abroad in recent years,the research advances in some rich functional constituents including isoflavones,proteins and peptides,carbohydrates,saponins and trace elements in chickpeas was reviewed in this paper. It provides a basis for the research,development and utilization of the functional constituents of chickpea in the future.

关键词： Chickpea Functional constituents Advances

肘管综合征的临床与解剖学研究（附62例分析）

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中华骨科杂志 1994年第12期14卷 755-758页

作者：马学义刘美音卢美源张明山东省千佛山医院山东省医科大学解剖教研究

本文对６２例肘管综合征手术治疗进行了分析，并对４０例尸体上肢的肘管及尺神经在肘部的解剖做了测量和观察。对三角弓状韧带做了分型和测量，以及尺神经在韧带上缘的变化，指出髁上横韧带的意义。本组病因以继发外伤最多３８例，肿瘤... 详细信息

本文对６２例肘管综合征手术治疗进行了分析，并对４０例尸体上肢的肘管及尺神经在肘部的解剖做了测量和观察。对三角弓状韧带做了分型和测量，以及尺神经在韧带上缘的变化，指出髁上横韧带的意义。本组病因以继发外伤最多３８例，肿瘤１３例，先天肘外翻４例，骨性关节炎３例等。主要病理改变在尺神经及弓状韧带。手术多行弓状韧带切开，尺神经前移，神经内外松解术。手术效果优良率９２．６％。有明显肌萎缩者虽经手术，但很难复原，其预后取决于神经受损程度。

关键词：肘管综合征三角弓状韧带肘外翻解剖学

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临床护理路径在输尿管镜气压弹道碎石术中的应用

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现代医院 2011年第12期11卷 80-81页

作者：卢媚媛东莞市石排医院广东东莞523330

目的探讨临床护理路径在输尿管镜下气压弹道碎石术患者中的应用,规范该类患者手术前后的医疗护理。方法将50例输尿管结石患者随机分为试验组和对照组,每组25例。对照组按常规护理模式管理,试验组应用临床护理路径管理,比较两组患者住院... 详细信息

目的探讨临床护理路径在输尿管镜下气压弹道碎石术患者中的应用,规范该类患者手术前后的医疗护理。方法将50例输尿管结石患者随机分为试验组和对照组,每组25例。对照组按常规护理模式管理,试验组应用临床护理路径管理,比较两组患者住院费用、住院天数、患者掌握相关知识程度和对护士的满意度及术后并发症发生率。结果试验组患者的平均住院天数、平均住院费用、术后并发症的发生率均较对照组少,对护士的满意度及疾病相关知识的掌握程度明显高于对照组患者(p<0.05),差异具有统计学意义。结论实施临床护理路径管理能显著缩短输尿管结石气压弹道碎石术患者平均住院日,降低住院费用,提高护理质量。

关键词：输尿管结石输尿管镜气压弹道碎石术临床护理路径

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黄花倒水莲3种成分含量测定及抗炎、调血脂作用研究

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药物评价研究 2024年第1期47卷 79-86页

作者：陆梅元廖国富雷虹谢臻李杉广西壮族自治区食品药品审评查验中心广西南宁530029 广西中医药大学广西南宁530201

目的建立黄花倒水莲醇Polygala fallax Hemsl.提取物中3种成分的含量测定方法;探讨黄花倒水莲醇提取物抗炎、调血脂作用。方法采用HPLC法测定黄花倒水莲醇提取物中3种成分——细叶远志皂苷、獐牙菜苦苷、芦丁的含量;建立二甲苯致小鼠... 详细信息

目的建立黄花倒水莲醇Polygala fallax Hemsl.提取物中3种成分的含量测定方法;探讨黄花倒水莲醇提取物抗炎、调血脂作用。方法采用HPLC法测定黄花倒水莲醇提取物中3种成分——细叶远志皂苷、獐牙菜苦苷、芦丁的含量;建立二甲苯致小鼠耳肿胀、冰醋酸致小鼠腹腔毛细血管通透性增加、角叉菜胶致小鼠足跖肿胀炎症模型,评价黄花倒水莲醇提取物高、中、低剂量(40、20、10 g·kg^(-1))改善急、慢性炎症的药理作用;建立二甲苯致摘除双侧肾上腺小鼠耳肿胀、角叉菜胶致摘除双侧肾上腺小鼠足跖肿胀模型,判断其抗炎作用是否依赖下丘脑-垂体-肾上腺轴(HPAA)系统,试剂盒法测定足跖炎症组织丙二醛(MDA)、一氧化氮(NO)水平和超氧化物歧化酶(SOD)活性;采用高脂饲料喂食SD大鼠建立高脂大鼠模型,考察黄花倒水莲醇提取物高、中、低剂量(40、20、10 g·kg^(-1))对高脂大鼠血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平的影响。结果 HPLC法方法学考察各项指标符合规定,细叶远志皂苷、獐牙菜苦苷、芦丁的平均加样回收率均在97.13%~101.82%,RSD值均小于3.0%;黄花倒水莲醇提取物中细叶远志皂苷、獐牙菜苦苷、芦丁的质量分数分别为121.71、84.02、52.53μg·g^(-1)。与模型组比较,黄花倒水莲醇提取物高、中剂量对小鼠耳肿胀有明显的抑制作用(P<0.05、0.01),高、中、低剂量对小鼠腹腔毛细血管通透增加均有显著抑制作用(P<0.05、0.01),高、中、低剂量对角叉菜胶造成的小鼠足跖肿胀都有明显的抑制作用(P<0.05、0.01)。与模型组比较,在摘除双侧肾上腺小鼠中,黄花倒水莲醇提取物高、中剂量能显著抑制二甲苯致该模型小鼠耳肿胀(P<0.05、0.01),显著抑制角叉菜胶致该模型小鼠足跖肿胀(P<0.05、0.01);黄花倒水莲醇提取物高、中、低剂量均能显著降低足跖中MDA、NO水平(P<0.05、0.01),均能显著提高足跖中SOD活性(P<0.05、0.01)。ig高脂饲料28 d后,与模型组比较,黄花倒水莲醇提取物高、中、低剂量能显著降低大鼠血清的TC、TG和LDL-C水平(P<0.05)。结论建立的HPLC含量测定方法专属性强、重复性好、准确度高,可用于黄花倒水莲醇提取物中细叶远志皂苷、獐牙菜苦苷、芦丁的含量测定;黄花倒水莲醇提取物具有抗炎活性,其抗炎作用的发挥不依赖HPAA系统,与减少MDA、NO水平,提高SOD活性有关;对高脂大鼠发挥明显的调血脂作用。

关键词：黄花倒水莲细叶远志皂苷獐牙菜苦苷芦丁抗炎调血脂

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健康教育对老年手术患者自我效能感的影响

现代临床护理 2013年第7期12卷 77-79页

作者：卢媚媛卢丹詹帅云东莞市石排医院广东东莞523330

目的探讨健康教育对老年手术患者自我效能感的影响。方法对120例老年手术患者实施行为干预、社会支持、疾病知识教育,比较健康教育前后患者自我效能感的差异。结果健康教育后老年患者日常生活、健康行为、药物治疗及遵医行为得分均较健... 详细信息

目的探讨健康教育对老年手术患者自我效能感的影响。方法对120例老年手术患者实施行为干预、社会支持、疾病知识教育,比较健康教育前后患者自我效能感的差异。结果健康教育后老年患者日常生活、健康行为、药物治疗及遵医行为得分均较健康教育前高(均P<0.05)。结论健康教育可增强老年手术患者的自我效能感,是提高患者治疗依从性,建立健康行为方式的有效方法。

关键词：手术患者健康教育自我效能感

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