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Improvement in Resolution of Multiphoton Scanning Structured Illumination Microscopy via Harmonics

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Engineering 2022年第9期16卷 65-72页

作者： Lei wang Xiaomin Zheng Jie Zhou meiting wang Jiajie Chen Youjun Zeng Gaixia Xu Ying wang Haixia Qiu Yonghong Shao Junle Qu Bruce Zhi Gao Ying Gu College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceShenzhen UniversityShenzhen 518060China Department of Laser Medicine First Medical Center of PLA General HospitalBeijing 100853China Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA

We describe a multiphoton(mP)-structured illumination microscopy(SIM)technique,which demonstrates substantial improvement in image resolution compared with linear SIM due to the nonlinear response of *** nonlinear response is caused by the effect of nonsinusoidal structured illumination created by scanning a sinusoidally modulated illumination to excite an mP fluorescence *** harmonics of the structured fluorescence illumination are utilised to improve *** present an mP-SIM theory for reconstructing the super-resolution image of the ***,the resolution of our m P-SIM is unlimited if all the high-order harmonics of the nonlinear response of fluorescence are ***,we demonstrate an 86 nm lateral resolution for two-photon(2P)-SIM and a 72 nm lateral resolution for second-harmonic-generation(SHG)-*** further demonstrate their application by imaging cells stained with F-actin and collagen fibres in mouse-tail *** method can be directly used in commercial mP microscopes and requires no specific fluorophores or high-intensity laser.

关键词： Super-resolution microscopy Structured illumination microscopy Multiphoton-structured illumination microscopy(SIM) SIM Second-harmonic-generation(SHG)-SIM

Comparison of point detection and area detection for point-scanning structured illumination microscopy

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Journal of Innovative Optical Health Sciences 2023年第4期16卷 134-149页

作者： Wenshuai Wu Jiajie Chen meiting wang Lei wang Xiaomin Zheng Jia Li Junle Qu Bruce Zhi Gao Yonghong Shao College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060GuangdongP.R.China Department of Bioengineering and COMSET Clemson University ClemsonSC 29634 USA

Structured illumination microscopy(SIM)is suitable for biological samples because of its relatively low-peak illumination intensity requirement and high imaging *** system resolution is affected by two typical detection modes:Point detection and area ***,a systematic analysis of the imaging performance of the different detection modes of the system has rarely been *** this study,we compared laser point scanning point detection(PS-PD)and point scanning area detection(PS-AD)imaging in nonconfocal microscopy through theoretical analysis and simulated *** results revealed that the imaging resolutions of PSPD and PS-AD depend on excitation and emission point spread functions(PSFs),***,we combined the second harmonic generation(SHG)of point detection(P-SHG)and area detection(A-SHG)with SIM to realize a nonlinear SIM-imaging technique that improves the imaging ***,we analytically and experimentally compared the nonlinear SIM performance of P-SHG with that of A-SHG.

关键词： Super-resolution structured illumination microscopy second harmonic generation

Case Retrieval Method of Multi-parameter for Customized Product

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武汉理工大学学报 2006年第S2期28卷 543-546页

作者： DING Junjian TAN Shili SONG Xiaofeng wang meiting (School of Mechanical&Electronic Engineering and Automation,Shanghai University,Shanghai 200072,China School of Mechanical&Electronic Engineering and Automation Shanghai UniversityShanghai 200072China

The CBR (Case-Based Reasoning) usually is been used to accomplish customized products by variant design or reusable *** the CBR,retrieve is very important.A simple case retrieval method is been brought forward to retrieve a opti- mal prototype based on using inputted multi-parameters,it can be programmed *** example has been proved this method can find optimal prototype for new design task efficiently.

关键词： case-based reasoning retrieve similar distance multi-parameter

Computing Free Energy of States and Potential of Mean Force at High Levels from Low Level Simulations

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Computing Free Energy of States and Potential of Mean Force ...

Comparison of Accuracy, Convergence and Efficiency between Nonequilibrium and Equilibrium Alchemical Transformations in the Relative Binding Free Energy Calculations

第十五届全国化学动力学会议

作者： Pengfei Li meiting wang Xiangyu Jia 梅晔 State Key Laboratory of Precision Spectroscopy School of Physics and Materials ScienceEast China Normal University CNYU-ECNU Center for Computational Chemistry at NYU Shanghai

Free energy calculations at high levels such as QM（ai）/MM level are very expensive, due that extensive phase space sampling is always indispensable before the convergence can be reached and for most cases many intermediate states are required to make the conversion between two states smooth enough. We proposed an efficient method for the calculations of free energies at QM/MM levels. In this method, the QM/MM free energies are computed indirectly via a series of MM states and the MM-to-QM corrections were computed at two end points using thermodynamics perturbation（TP）. This method provides the minimal variance for the results if expensive QM/MM simulations are avoided. We also proposed a quantity called reweighting entropy, which measures the reliability of TP calculations. Similarly, calculations of potentials of mean force（PMF）, which are essential to the understanding of reaction mechanisms, are also expensive. We also proposed an efficient method for the calculations of PMF at high QM/MM levels using phase space sampling under low level Hamiltonians followed by free energy corrections.

关键词： free energy potential of mean force QM/MM WHAM TP

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Comparison of Accuracy, Convergence and Efficiency between N...

疫情下中国宏观杠杆率的新变化、风险与应对策略

第十三届全国量子化学会议

作者： Pengfei Li Xiangyu Jia meiting wang Ye Mei State Key Laboratory of Precision Spectroscopy School of Physics and Materials ScienceEast China Normal University NYU-ECNU Center for Computational Chemistry at NYU Shanghai

In the past few decades, much effort has been devoted to estimate accurate protein-ligand binding affinity, which is a central challenge in structure-based drug *** the equilibrium free energy estimators, such as free energy perturbation（FEP） method, thermodynamic integration（TI） method,Bennett’s acceptance ratio（BAR） and so on, require that molecular dynamic simulations at each states including many immediate states reach equilibrium and the structure sampling is uncorrelated. These need a large computational cost to get the converged free energy calculations. Rather than spending large computational costs on long equilibrium sampling, in 1997,Jarzynski put forward an equality to calculate the free energy difference between the two states, which needed to carry out many shorter nonequilibrium simulations from state A to state ***, in contrast to equilibrium free energy calculations, how the performance of the nonequilibrium free energy calculations in the relative binding free energy calculations is not clear *** known complex systems with respective different ligands are chosen to calculate the relative binding affinity for comparing the performance of nonequilibrium and equilibrium *** is T4-lysozyme mutant L99A（L99A） system. Another one is cyclooxygenase-2（COX-2） system.

关键词： Free energy Relative binding free energy Drug design Nonequilibrium method Alchemical transformation

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新金融 2021年第3期 25-31页

作者：王梅婷北京大学经济学院博士后流动站中国银行股份有限公司博士后工作站

2020年在新冠疫情冲击下,逆周期调控政策推动宏观杠杆率快速攀升,表明自2015年起的去杠杆进程告一段落。与2008-2014年加杠杆阶段相比,2020年各部门加杠杆的结构、渠道、期限出现新变化。非金融企业部门加杠杆的主体发生变化,国企杠杆... 详细信息

2020年在新冠疫情冲击下,逆周期调控政策推动宏观杠杆率快速攀升,表明自2015年起的去杠杆进程告一段落。与2008-2014年加杠杆阶段相比,2020年各部门加杠杆的结构、渠道、期限出现新变化。非金融企业部门加杠杆的主体发生变化,国企杠杆率趋于下降,私营企业负债快速增加。"房住不炒"下居民平稳加杠杆,贷款增速有所回落。政府部门对宏观杠杆率增幅贡献率上升,中央政府承担支出责任的主动性提高,地方政府市场化融资水平提升。总的来看,宏观杠杆率快速上升缓冲了疫情负面影响,促进金融服务实体经济,但也提高了企业、居民债务风险,加重了地方财政收支平衡的压力。当前,要处理好稳增长和防风险的关系,稳定宏观杠杆率,有序处置各部门债务风险,健全多层次资本市场,提高直接融资占比。

关键词：宏观杠杆率新变化杠杆结构债务风险

冠突散囊菌胞内提取物抑制恶臭假单胞菌的作用机制

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食品科学 2023年第7期44卷 57-64页

作者：张雯王梅婷王明钰倪莉福州大学食品科学技术研究所福建福州350108

本实验对冠突散囊菌FS-1菌丝体中抑菌活性物质进行提取和鉴定,并以恶臭假单胞菌LP-3为抑菌模型,利用冠突散囊菌FS-1菌丝体提取物对其进行处理,通过抑菌活性测定、微观结构观察、恶臭假单胞菌LP-3细胞内容物分析以及代谢组学分析,研究冠... 详细信息

本实验对冠突散囊菌FS-1菌丝体中抑菌活性物质进行提取和鉴定,并以恶臭假单胞菌LP-3为抑菌模型,利用冠突散囊菌FS-1菌丝体提取物对其进行处理,通过抑菌活性测定、微观结构观察、恶臭假单胞菌LP-3细胞内容物分析以及代谢组学分析,研究冠突散囊菌FS-1胞内提取物的抑菌机理。结果表明,冠突散囊菌FS-1菌丝体的乙酸乙酯提取物(FS-1 ethyl acetate extract,FSEAE)对1×10^(4) CFU/mL恶臭假单胞菌LP-3的半抑制浓度为8 mg/mL。扫描电子显微镜观察结果表明,FSEAE破坏了LP-3的细胞膜;对LP-3上清液中钾(K^(+))含量、细胞代谢途径中的氧化应激酶系(超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶)活性和丙二醛含量测定结果显示,FSEAE的抑菌作用主要发生在LP-3的对数生长期(8~16 h)。基于非靶向代谢组学分析FSEAE处理组和对照组(二甲基亚砜处理)样本,得到124种代谢产物,其中30种具有显著差异的代谢产物,经京都基因与基因组百科全书富集分析得出FSEAE主要通过干扰三羧酸循环影响恶臭假单胞菌LP-3的生长和代谢。本研究可为冠突散囊菌及其代谢产物的开发利用提供理论依据。

关键词：冠突散囊菌抑菌机制恶臭假单胞菌有机提取物代谢组学分析

光纤Bragg光栅应变测量中啁啾特性的研究

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光子学报 2006年第6期35卷 877-880页

作者：童凯汪梅婷李志全燕山大学电气工程学院秦皇岛066004 燕山大学继续教育学院秦皇岛066004

用光纤Bragg光栅进行应变(静态、动态)测量时,由于光栅轴向各部分受到的应变不同,因而产生布喇格中心波长漂移量发生变化·实际测量中往往忽略此扰动对光纤光栅反射谱中心波长的影响,则必然带来测量误差·采用耦合模理论,分析并研究了Br... 详细信息

用光纤Bragg光栅进行应变(静态、动态)测量时,由于光栅轴向各部分受到的应变不同,因而产生布喇格中心波长漂移量发生变化·实际测量中往往忽略此扰动对光纤光栅反射谱中心波长的影响,则必然带来测量误差·采用耦合模理论,分析并研究了Bragg光栅受轴向线性应变、高次应变和非线性周期应变波等情况下的啁啾特性·根据各自啁啾特性的不同,给出了对光纤光栅反射谱中心的影响,以及测量各种应变时的测量条件和范围·

关键词：光纤光栅啁啾光栅应变反射谱

An Efficient Strategy for the Calculations of Free Energies at QM/MM Level

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An Efficient Strategy for the Calculations of Free Energies ...