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Changes in the epigenome and transcriptome of rice in response to Magnaporthe oryzae infection

The Crop Journal 2021年第4期9卷 843-853页

作者： Na Cui Xiaofeng Chen Yan Shi meirong chi Jiantuan Hu Kunlong Lai Zonghua Wang Haifeng Wang State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops College of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Marine and Agricultural Biotechnology Laboratory Institute of OceanographyMinjiang UniversityFuzhou 350108FujianChina Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Key Lab of Sugarcane BiologyCollege of AgricultureGuangxi UniversityNanning 530004GuangxiChina

DNA methylation participates in regulating the expression of coding and non-coding regions in plants. To investigate the association between DNA methylation and pathogen infection, we used whole-genome bisulfite sequencing to survey temporal DNA methylation changes in rice after infection with the rice blast fungus Magnaporthe oryzae. In contrast to previous findings in Arabidopsis, global DNA methylation levels in rice increased slightly after rice blast infection. We identified over 38,000 differentially methylated regions(DMRs), and hypermethylated DMRs far outnumbered hypomethylated DMRs. Most DMRs were located in transposable element regions. Using transcriptome analysis, we identified 8830 differentially expressed genes(DEGs) after 1, 3, and 5 days of infection. Over one-third of DEGs, most of which were CHH-type DMRs, were associated with DMRs. Functional analysis of the CHH DMR-DEGs indicated their involvement in many important biological processes, including cell communication and response to external stimulus. The transcription of many NBS-LRR family genes was affected by changes in DNA methylation, suggesting that DNA methylation plays essential roles in the response of rice to M. oryzae infection. More broadly, the DNA methylation analysis presented here sheds light on epigenomic involvement in plant defense against fungal pathogens.

关键词： DNA methylation Epigenome Magnaporthe oryzae Rice Transcriptome

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Epigenetic modification mechanisms of chloroplasts mutants in pineapple leaves during somatic regeneration

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Horticultural Plant Journal 2023年第3期9卷 509-522页

作者： Yanhui Liu S.V.G.N.Priyadarshani meirong chi Maokai Yan Mohammad Aqa Mohammadi Man Zhang Qiao Zhou Lulu Wang Tiantian Luo Myat Hnin Wai Xiaomei Wang Hanyang Cai Haifeng Wang Yuan Qin College of Life Sciences Key Lab of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyCenter for Genomics and BiotechnologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China College of Life Science Longyan UniversityLongyanFujian 364012China Guangxi Key Laboratory of Sugarcane Biology State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureGuangxi UniversityNanningGuangxi 530004China Horticulture Research Institute Guangxi Academy of Agricultural SciencesNanning Investigation Station of South Subtropical Fruit TreesMinistry of AgricultureNanningGuangxi 530007China Pingtan Science and Technology Research Institute of Fujian Agriculture and Forestry University FuzhouFujian 350002China National Education Commission Sri Lanka

Somaclonal variation in tissue culture is a common phenomenon induced by various external or internal environmental conditions,resulting in heritable or non-heritable alterations in gene expression. One crucial mechanism involved in plant growth and development is epigenetic regulation. A highly dynamic epigenome can respond to environmental changes by regulating gene expression. DNA methylation is one of these epigenetic modifications that can alter gene expression in tissue-cultured pineapple plants. The underlying mechanism of such somaclonal variations in pineapple and the epigenetic regulation involvement in somaclonal variations has not been studied. This study performed DNA methylome and transcriptome sequencing of wild-type(WT) and mutant pineapple plants(WS, HW, and TW). We observed altered DNA methylation patterns in chlorophyll development in the mutants. Specifically, we noticed that the methylation levels in the CHG and CHH contexts were lower in the gene body regions compared to the upstream and downstream regions. We identified several thousand differentially methylated regions(DMRs) located at the gene body regions, some of which overlapped with the differentially expressed genes(DEGs). Functional enrichment analyses suggested that these genes were involved in chlorophyll metabolism. Thus, our results revealed that the transcriptional regulation of many chlorophyll metabolic essential genes could be regulated by DNA methylation caused by somaclonal variations and provided insights into epigenetic mechanisms underlying the regulation of chlorosis in pineapple plants.

关键词： Pineapple DNA methylation Transcriptome Chlorophyll metabolism

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Application of biofl oc technology in recirculation A rtemia culture system

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Journal of Oceanology and Limnology 2022年第4期40卷 1669-1677页

作者： Xuejiao LIANG chi ZHANG Dongdong DU meirong GAO Liying SUI Key Laboratory of Marine Resource Chemistry and Food Technology(TUST) Ministry of EducationCollege of Marine and Environmental SciencesTianjin University of Science and TechnologyTianjin 300457China Institute of Fisheries Science Tibet Academy of Agricultural and Animal Husbandry SciencesLhasa 850000China

Biofl oc technology(BFT)improves water quality,and productivity of the farmed species through converting ammonium nitrogen to microbial protein,stabilizing microbial community,and reducing the production *** this study,a small-scale biofl oc development unit was designed in combination of recirculation system(RAS)for Artemia *** growth,water quality,and microbial composition of biofl ocs in RAS were studied in comparison with in-situ batch culture(Glu).Glucose was added in RAS and Glu at C/N ratio of *** cultures without glucose addition,but with 50%daily water renewal(WRe)and without water renewal(NWRe)were considered as the *** mia were cultured at 25℃ and salinity 30 for 24 days and fed formulated *** results showed that compared to the controls,Glu signifi cantly improved the Artemia biomass,increased the biofl oc volume,and reduced the content of total ammonia nitrogen(TAN),nitrite nitrogen(NO_(2)-N)and nitrate nitrogen(NO_(3)-N)in water column(P<0.05).In addition,RAS had similar results with *** throughput sequencing analysis on biofl oc microbial composition demonstrated that glucose supplement shaped the microbial community structure,and increased proportion of potential probiotic bacteria and suppressed pathogenic bacteria ***,we analyzed the relationship between the microbial composition of biofl oc and environmental *** correspondence analysis(CCA)indicated that inorganic nitrogen in culture water had great impact on biofl oc microbial composition in NWRe and WRe,whilst the dissolved organic carbon(DOC)modifi ed the microbial community in Glu and *** study shows the advantages of BFT in Artemia culture and provides practical information for applying BFT-RAS in indoor Artemia culture.

关键词： biofloc technology Artemia water quality microbial community structure

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全凭静脉全身麻醉与静脉复合七氟烷吸入全身麻醉在小儿麻醉中的安全性比较

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中国当代医药 2024年第26期31卷 84-88页

作者：迟庆胜冯伟徐志秀刘美蓉山东省日照市中医医院麻醉科山东日照276800 山东省日照市中医医院胃肠外科山东日照276800 山东省日照市中医医院手术室山东日照276800 山东省日照市中医医院超声三科山东日照276800

目的探讨小儿麻醉中实施全凭静脉全身麻醉与静脉复合七氟烷吸入全身麻醉的效果。方法选取2022年9月至2023年9月日照市中医医院收治的60例手术患儿作为研究对象,采用随机数字表法将其分为对照组和观察组,各30例。对照组患儿采用丙泊酚全... 详细信息

目的探讨小儿麻醉中实施全凭静脉全身麻醉与静脉复合七氟烷吸入全身麻醉的效果。方法选取2022年9月至2023年9月日照市中医医院收治的60例手术患儿作为研究对象,采用随机数字表法将其分为对照组和观察组,各30例。对照组患儿采用丙泊酚全凭静脉全身麻醉,观察组患儿采用静脉复合七氟烷吸入全身麻醉。比较两组患儿的血流动力学指标、术后苏醒情况、应激反应指标、谵妄发生情况和不良反应发生情况。结果观察组术中的平均动脉压(MAP)、心率(HR)以及术中、术后20 min的血氧饱和度(SpO_(2))高于对照组,差异有统计学意义(P<0.05)。观察组的拔管时间、定向力恢复时间、苏醒时间短于对照组,插管时间、意识消失时间长于对照组,差异有统计学意义(P<0.05)。观察组术后20 min、气管拔管后的血清去甲肾上腺素(NE)、肾上腺素(E)、皮质醇(Cor)高于对照组,差异有统计学意义(P<0.05)。观察组术后3 d的儿科麻醉后苏醒期谵妄(PAED)量表评分低于对照组,差异有统计学意义(P<0.05)。观察组的不良反应总发生率低于对照组,差异有统计学意义(P<0.05)。结论小儿麻醉中实施静脉复合七氟烷吸入全身麻醉方式,可更好地稳定术中血流动力学,术后快速苏醒及恢复,且减少术后谵妄的发生,安全性有保障,但丙泊酚全凭静脉麻醉可减轻围手术期的应激反应。

关键词：全凭静脉全身麻醉静脉复合麻醉七氟烷吸入小儿麻醉

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Microbial activity was greater in soils added with herb residue vermicompost than chemical fertilizer

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Soil Ecology Letters 2020年第3期2卷 209-219页

作者： meirong Lv Jingjuan Li Weixin Zhang Bo Zhou Jun Dai chi Zhang The College of Natural Resources and Environment South China Agricultural UniversityGuangzhou 510650China Institute of Oceanographic Instrumentation Qilu University of Technology(Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Marine Monitoring Instrument Equipment TechnologyNational Engineering and Technological Research Center of Marine Monitoring EquipmentQingdao 260000China Agricultural Technology Promotion Center of Huicheng District Huizhou 516008China Key Laboratory of Geospatial Technology for Middle and Lower Yellow River Regions Ministry of Education/College of Environment and PlanningHenan UniversityKaifeng 475004China Tea Research Institute Guangdong Academy of Agricultural SciencesGuangzhou 510650China

Herb residue vermicompost is thought to have high agriculture value,while its effects on soil microbial activities have not been fully ***,soil microbial biomass,respiration and enzyme activities in soil planted with maize were compared among treatments amended with herb residue vermicompost at rates of 25,50,75 and 100 g kg^(-1),chemical NPK fertilizer and no fertilizer(the control).Our results showed that soil microbial biomass carbon,respiration,and alkaline phosphatase,urease,and invertase activities were greater in soil amended with herb residue vermicompost than the unfertilized control(P<0.05).Compared with chemical fertilizer,herb residue vermicompost increased soil urease and alkaline phosphatase activities at each application rate,promoted soil respiration and microbial biomass carbon at the application rates of 50,75 and 100 g kg^(-1),and increased soil inverse activity at the application rates of 75 and 100 g kg^(-1).In conclusion,herb residue vermicompost supported greater soil microbial biomass,respiration and enzyme activities than conventional NPK fertilizer,and the effect was larger when higher rates of herb residue vermicompost were added.

关键词： Soil Herb residue vermicompost Microbial biomass Respiration Enzyme activity

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保元汤合桃红四物汤治疗对气虚血瘀型急性STEMI患者PCI术后左心室血流动力学的影响

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江苏医药 2024年第9期50卷 939-941页

作者：刘美蓉迟庆胜茹翱王新飞李艳君日照市中医医院超声科山东276800 日照市中医医院麻醉手术室山东276800 日照市中医医院心内科山东276800 湖州市第一人民医院超声科浙江

目的探讨保元汤合桃红四物汤治疗对气虚血瘀型急性ST段抬高型心肌梗死(STEMI)患者冠状动脉介入(PCI)术后左心室血流动力学的影响。方法气虚血瘀型急性STEMI患者60例PCI术后随机分为两组,每组30例。观察组在标准化常规药物治疗基础上,... 详细信息

目的探讨保元汤合桃红四物汤治疗对气虚血瘀型急性ST段抬高型心肌梗死(STEMI)患者冠状动脉介入(PCI)术后左心室血流动力学的影响。方法气虚血瘀型急性STEMI患者60例PCI术后随机分为两组,每组30例。观察组在标准化常规药物治疗基础上,口服保元汤合桃红四物汤治疗1周。对照组仅给予标准化常规药物治疗1周。采用超声心动图检测两组治疗前后左心室血流动力学指标。结果与治疗前和对照组治疗后比较,观察组治疗后左心室射血分数、短轴缩短率、每搏输出量、主动脉瓣口血流速度、二尖瓣血流峰速度(E)和二尖瓣环速度(e′)均升高,而E/e′比值降低(P<0.05或P<0.01)。结论保元汤合桃红四物汤可改善气虚血瘀型急性STEMI患者PCI术后左心室血流动力学。

关键词：保元汤桃红四物汤急性ST段抬高型心肌梗死

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The“terminal line”:a novel sign for the identification of distal mesorectum end during TME for rectal cancer

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Gastroenterology Report 2022年第1期10卷 468-475页

作者： Waleed M.Ghareeb Xiaojie Wang Xiaozhen Zhao meirong Xie Sameh H.Emile Sherief Shawki Pan chi Department of Colon and Rectal Surgery Union Hospital of Fujian Medical UniversityFuzhouFujianP.R.China Department of Surgery Faculty of MedicineSuez Canal UniversityIsmailiaEgypt Department of Colon and Rectal Surgery Mayo ClinicRochesterMNUSA Laboratory of Clinical Applied Anatomy Fujian Medical UniversityFuzhouFujianP.R.China Basic Medical College Fujian Medical UniversityFuzhouFujianP.R.China Department of Colorectal Surgery Cleveland Clinic FloridaWestonFLUSA Colorectal Surgery Unit General Surgery DepartmentMansoura University HospitalsMansouraEgypt

Background:Although the clinical importance of complete,intact total mesorectal excision(TME)is the widely accepted standard for decreasing local recurrence of rectal cancer,the residual mesorectum still represents a significant component of resection margin *** study aimed to use a visible intraoperative sign to detect the distal mesorectal end to ensure complete inclusion of the mesorectum and avoid unnecessary ***:The distal mesorectum end was investigated retrospectively through a review of 124 operative videos at the Union Hospital of Fujian Medical University(Fujian,china)and Cleveland Clinic(Ohio,USA)by two independent surgeons who were blinded to each ***,28 cadavers and 44 post-operative specimens were prospectively examined by hematoxylin and eosin(H&E)staining and Masson’s staining to validate and confirm the findings of the retrospective *** and multivariate analyses were carried out to detect the independent factors that can affect the visualization of the distal mesorectal ***:The terminal line(TL)is the distal mesorectal end of the transabdominal and transanal TME(taTME)and appears as a remarkable pearly white fascial structure extending posteriorly from 2 to 10 o’*** examination revealed that the fascia propria of the rectum merges with the presacral fascia at the TL,beyond which the mesorectum ends,with no further downward *** the retrospective observation,the TL was seen in 56.6%of transabdominal TME and 56.0%of taTME *** approach and tumor distance from the anal verge were the independent variables that directly influenced the detection of the TL(P=0.03 and P=0.01).Conclusion: The TL is a visible sign where the transabdominal TME should end and the taTME should begin. Recognitionof the mesorectal end may impact the certainty of complete mesorectum inclusion. Further clinical trials are needed toconfirm the preliminary findings.

关键词： rectal cancer total mesorectal excision taTME TAMIS laparoscopy robotic

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常规化学指标结合卡方概率密度函数的烟叶质量评价模型构建与应用

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烟草科技 2024年第4期57卷 18-23页

作者：马翔王萝萍钱颖颖田栾栾殷美荣刘虎斌李军会云南中烟工业有限责任公司原料中心昆明市五华区龙泉路548号650231 红塔集团玉溪卷烟厂云南省玉溪市红塔区红塔大道118号653100 中国农业大学信息与电气工程学院北京市海淀区学院路100083

为了更加科学、系统、高效地实现烟叶相对精准、快速的分级,以还原糖总氮比(Kovalenko指数)、总氮烟碱比和烟碱质量分数作为烟叶质量的主要评价指标,基于具有非线性、非单调、非对称特点的卡方函数,以及不同外观和感官质量等级的复烤模... 详细信息

为了更加科学、系统、高效地实现烟叶相对精准、快速的分级,以还原糖总氮比(Kovalenko指数)、总氮烟碱比和烟碱质量分数作为烟叶质量的主要评价指标,基于具有非线性、非单调、非对称特点的卡方函数,以及不同外观和感官质量等级的复烤模块化学指标的分布规律,建立了针对云南产区烟叶内在质量的评价模型。基于建立的评价模型和近红外光谱检测数据,对红塔集团不同复烤模块烟叶的内在质量进行评价。结果表明:①质量评价结果与烟叶外观以及感官质量分类的趋势高度相关。②将化学指标优劣互补(例如,烟碱质量分数高的上部烟和烟碱质量分数低的下部烟)且感官评价分数较低的烟叶以一定比例均匀混合之后评价分数均有不同程度的提升,其中,BO与XL等级烟叶配伍可最大程度地提高烟叶的内在质量。③所建立的评价模型能够通过少数关键化学指标实现烟叶内在质量的快速、高效分级。应用工业分级烟叶的内在质量评价结果可为复烤模块配方提供指导。

关键词：烟叶近红外光谱法内在质量化学指标卡方函数评价模型

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chitosan/LiCl composite scaffolds promote skin regeneration in full-thickness loss

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Science china(Life Sciences) 2020年第4期63卷 552-562页

作者： Jifang Yuan Qian Hou Deyun Chen Lingzhi Zhong Xin Dai Ziying Zhu meirong Li Xiaobing Fu Wound Healing and Cell Biology Laboratory Institute of Basic Medical ScienceChinese PLA General HospitalBeijing 100853China Laboratory Animal Center Chinese PLA General HospitalBeijing 100853China Trauma Treatment Center Central LaboratoryChinese PLA General Hospital Hainan BranchSanya 572014China

Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin *** can modulate various biologic processes,promoting proliferation,angiogenesis,and decreasing ***,its role in skin repair is rarely *** this study,we loaded lithium chloride(LiCl)into the chitosan(chi)hydrogel and develop a sterile and biocompatible sponge scaffold through ***-vitro assessment demonstrated that the chi-LiCl composite scaffolds(CLiS)possessed favorable cytocompatibility,swelling and *** created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of *** with the wounds of control and chi scaffold(CS)groups,the wounds in the CLiS-treated group showed reduced inflammation,improved angiogenesis,accelerated re-epithelialization,sustained high expression ofβ-catenin with a small amount of regenerated hair ***,CLiS may be a promising therapeutic dressing for skin wound repair and regeneration.

关键词： wound healing chitosan LiCl small molecule skin regeneration

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基于“一气周流”理论探讨干眼的病因病机及证治

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中国中医眼科杂志 2024年第8期34卷 762-765页

作者：邱惠泉朱晓林陈美荣王聪敏仇梓璇尹荣娟山东中医药大学济南250014 山东中医药大学附属医院济南250011

干眼作为一种发病率持续上升的眼科疾病,严重影响着患者的视觉及生活质量。西医认为其病因及发病机制复杂,疗效欠佳。本文基于“一气周流”理论,结合“五轮学说”,认为干眼的病因病机主要在于脾土之不升、肺金失于清降、肝木郁而不达、... 详细信息

干眼作为一种发病率持续上升的眼科疾病,严重影响着患者的视觉及生活质量。西医认为其病因及发病机制复杂,疗效欠佳。本文基于“一气周流”理论,结合“五轮学说”,认为干眼的病因病机主要在于脾土之不升、肺金失于清降、肝木郁而不达、心火化热灼津、肾中坎阳失藏,治疗宜从健脾、润肺、疏肝、养心及滋肾入手,兼顾脏腑间的相关性,从而恢复一气周流的生理模式,可为中医治疗干眼提供参考。

关键词：干眼一气周流五轮学说病因病机证治

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