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Naturally occurring spike mutations influence the infectivity and immunogenicity of SARS-CoV-2

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Cellular & Molecular Immunology 2022年第11期19卷 1302-1310页

作者： Qiaoli Peng Runhong Zhou Na Liu Hui Wang Haoran Xu meiqing zhao Dawei Yang Ka-Kit Au Haode Huang Li Liu Zhiwei Chen AIDS Institute Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionPR China National Clinical Research Center for Infectious Diseases HKU AIDS Institute Shenzhen Research LaboratoryThe Third People’s Hospital of Shenzhen and The Second Affiliated Hospital of Southern University of Science and TechnologyShenzhenGuangdongPR China Department of Microbiology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionPR China Centre for Virology Vaccinology and Therapeutics LimitedThe University of Hong KongHong Kong Special Administrative RegionPR China HKU AIDS Institute Joint Laboratory Department of Clinical Microbiology and Infection ControlThe University of Hong Kong-Shenzhen HospitalShenzhenGuangdongPR China State Key Laboratory of Emerging Infectious Disease The University of Hong KongHong Kong Special Administrative RegionPR China

Mutations in SARS-CoV-2 variants of concern(VOCs)have enhanced transmissibility and immune evasion with respect to current vaccines and neutralizing antibodies(NAbs).How naturally occurring spike mutations affect the infectivity and antigenicity of VOCs remains to be *** entry efficiency of individual spike mutations was determined in vitro using pseudotyped ***/c mice were immunized with 2-dose DNA vaccines encoding B.1.1.7,B.1.351,B.1.1.529 and their single *** and humoral immune responses were then compared to determine the impact of individual mutations on *** the B.1.1.7 lineage,Del69–70 and Del 144 in NTD,A570D and P681H in SD1 and S982A and D1118H in S2 significantly increased viral entry,whereas T716I resulted in a *** the B.1.351 lineage,L18F and Del 242–244 in the NTD,K417N in the RBD and A701V in S2 also increased viral entry.S982A weakened the generation of binding *** sera showed reduced cross-neutralization activity against B.1.351,B.1.617.2(Delta)and B.1.1.529(Omicron BA.1).S982A,L18F,and Del 242–244 hindered the induction of cross-NAbs,whereas Del 69–70,Del144,R246I,and K417N showed the opposite effects.B.1.351 elicited adequate broad cross-NAbs against both B.1.351 and *** immunogens tested,however,showed low neutralization against circulating *** addition,T-cell responses were unlikely affected by mutations tested in the *** conclude that individual spike mutations influence viral infectivity and vaccine *** VOC-targeted vaccines is likely necessary to overcome immune evasion from current vaccines and neutralizing antibodies.

关键词： SARS-CoV-2 Variants of Concern Single mutation neutralizing antibody T cell responses vaccine immunogenicity

A Study of Molecular Resection Margins for Esophageal Squamous Cell Carcinoma Using Large Pathologic Sections

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Chinese Journal of Clinical Oncology 2006年第5期3卷 315-321页

作者： Guowei Ma Xu Zhang JunyeWang Qiuliang Wu Hao Long Peng Lin Jianhua Fu Richard Malthaner meiqing zhao Lanjun Zhang Zhesheng Wen Tiehua Rong State Key Laboratory of Oncology in Southern China GuangzhouGuangdong 510060 China. Department of Thoracic Surgery Cancer Center Sun Yat-Sen UniversityGuangzhou Guangdong 510060 China. Pathological Department Cancer Center Sun Yat-Sen University GuangzhouGuangdong 510060 China. Division of Thoracic Surgery and Surgical Oncology London Health Sciences Centre University of Western OntarioCanada.

OBJECTIVE It has been shown that application of molecular biological techniques to surgical margins of some cancers could predict risk of local recurrence. However, the optimal length of surgical resection with tumor-free surgical margins for esophageal squamous cell carcinoma （ES- CC） is unknown. This study was conducted to evaluate the optimal length of surgical resection for ESCC with molecularly tumor-free surgical margins marked by p53 and Ki67.METHODS Surgical specimens from 70 patients with ESCC were collected for study. The lengths of the upper margin, tumor, and lower margin of every specimen were measured during the operation. Each specimen was divided into three large pathologic sections, stained with H＆E and immunohistochemically for p53 and Ki67, and examined microscopically. The lengths of the upper and lower resection ends were measured for p53 and Ki67 positive expression. The actual surgical lengths were calculated by the principle of rational shrinkage.RESULTS All surgical margins were histologically tumor-free, while the positive rates of p53 and Ki67 were 66% and 54%. The positive rates of p53 and Ki67 in the upper resecti0n end were 17% and 20%. The mean lengths of the upper resection end showing p53 and Ki67 positive expression were 1.08±1.12 cm and 1.64±1.01 cm, and the maximum lengths were 3.73 cm and 3.26 cm. The positive,rates of p53 and Ki67 in the lower resection end were 20% and 23%. The mean lengths of the lower resection end of p53 and Ki67 with positive expression were 1.11±1.15 cm and 1.34±0.94 cm, and the maximum lengths were 3.73 cm and 3.61 cm.CONCLUSION The optimal length of surgical resection with molecularly tumor-free surgical margins of ESCC is not more than 5 cm.

关键词： esophageal cancer surgery pathology molecular biology.

平衡阀对掘进机液压平衡回路稳定性的影响研究

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机床与液压 2017年第1期45卷 150-155页

作者：赵美卿王栋山西工程技术学院山西阳泉045000

以螺纹插装式平衡阀为研究对象,分析其结构参数对掘进机截割部升降过程中液压平衡回路稳定性的影响。建立截割部的静力学方程,对截割部油缸负载进行模拟仿真,运用AMESim对平衡阀动态过程进行仿真,搭建试验台并进行平衡阀动态性能试验,利... 详细信息

以螺纹插装式平衡阀为研究对象,分析其结构参数对掘进机截割部升降过程中液压平衡回路稳定性的影响。建立截割部的静力学方程,对截割部油缸负载进行模拟仿真,运用AMESim对平衡阀动态过程进行仿真,搭建试验台并进行平衡阀动态性能试验,利用FLUENT对平衡阀阀芯开口处流场进行仿真。结果表明:油缸负载随着截割头的升高逐渐减小,动态性能的仿真与试验结果基本一致,较小的负重、阻尼孔和弹簧预紧力,以及较大的弹簧系数,有利于系统的稳定;较大的阀芯锥角和较小的开口度,有利于减少和避免气蚀和低频抖动现象发生。

关键词：螺纹插装式平衡阀液压平衡回路动态特性流场模拟掘进机

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分层注水定量配水工艺技术研究与应用

石油钻采工艺 2000年第4期22卷 63-65页

作者：赵振旺王春耘赵梅庆华北油田公司第四采油厂河北廊坊065007

因压力波动等因素的影响，普通的井下偏心堵塞器暴露出调配工作量及调配难度大、单层配水量的精度难以满足要求等问题。针对这一现状，研究开发了定量注水工艺技术──井下分注定量配水及地面定量配水配套技术。该技术应用DL-1差压式定... 详细信息

因压力波动等因素的影响，普通的井下偏心堵塞器暴露出调配工作量及调配难度大、单层配水量的精度难以满足要求等问题。针对这一现状，研究开发了定量注水工艺技术──井下分注定量配水及地面定量配水配套技术。该技术应用DL-1差压式定量堵塞器实现了注水井的井下定量注水，使调配过程一次成功，大大减轻了投捞测试工作量。同时，地面自动恒流装置的应用，实现了注水井的地面分注。文中介绍了该技术的原理及现场应用情况。

关键词：分层注水堵塞器控水定量配水油田注水

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神东矿区高产高效矿井通风优化设计探索

内蒙古煤炭经济 2005年第2期 57-58页

作者：赵美清神东煤炭公司科技中心设计院内蒙古鄂尔多斯市017000

本文阐述了榆家梁煤矿通风设计优化 ,通过对总回风大巷断面优化 ,不但保证矿井的安全生产。

关键词：高产高效通风优化

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Z2880A型滚丝机液压系统的改造

河北工业科技 1999年第2期16卷 52-54页

作者：赵美卿阳泉煤炭专科学校阳泉045001

Ｚ２８８０Ａ型滚丝机的液压系统采用节流调速方式，系统功率损失大，传动速度不稳定；采用容积节流调速方式，并在系统中加装压力补偿器，则系统速度稳定性好。

关键词：节流调速回路滚丝机液压系统

基于FLUENT掘进机插装式平衡阀多物理场耦合仿真分析

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现代制造技术与装备 2021年第4期57卷 128-130页

作者：赵美卿山西工程技术学院阳泉045000

针对掘进机用螺纹插装式平衡阀易产生节流发热问题,对螺纹插装式平衡阀的内部流场特性进行液固热耦合仿真分析。根据实际使用的插装式平衡阀建立整体三维模型,包括阀芯和阀体,对流体速度场和温度场进行仿真分析,以获得固液温度变化规律... 详细信息

针对掘进机用螺纹插装式平衡阀易产生节流发热问题,对螺纹插装式平衡阀的内部流场特性进行液固热耦合仿真分析。根据实际使用的插装式平衡阀建立整体三维模型,包括阀芯和阀体,对流体速度场和温度场进行仿真分析,以获得固液温度变化规律。根据热应力场和机械应力场的共同作用机制得到综合变形规律,从而为插装式平衡阀设计提供参考依据。

关键词：螺纹插装式平衡阀液固热耦合流场模拟 FLUENT

高锰酸钾改性活性炭对Au^(3+)的吸附性能研究

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河北师范大学学报（自然科学版） 2010年第3期34卷 329-331页

作者：于化江张立艳赵梅青沧州师范学院化学系河北沧州061001 河北师范大学化学与材料科学学院河北石家庄050016

以高锰酸钾改性的颗粒活性炭(MAC)为吸附剂,研究了pH值、吸附时间、MAC投加量和Au3+浓度对Au3+吸附性能的影响,并测定了吸附等温线.结果表明,随着pH的升高MAC对Au3+的吸附率先升高后降低,在pH=2.5时吸附率达到98%;在体系25℃,pH=2.5的... 详细信息

以高锰酸钾改性的颗粒活性炭(MAC)为吸附剂,研究了pH值、吸附时间、MAC投加量和Au3+浓度对Au3+吸附性能的影响,并测定了吸附等温线.结果表明,随着pH的升高MAC对Au3+的吸附率先升高后降低,在pH=2.5时吸附率达到98%;在体系25℃,pH=2.5的条件下,MAC对Au3+的吸附率随时间的延长而增大,吸附平衡时间为90 min;Au3+的吸附量随MAC投加量和Au3+浓度的增加而增大.对Au3+的吸附符合Langmuir单分子层吸附规律,单分子层饱和吸附量为0.332 4 mmol/g.

关键词：改性活性炭高锰酸钾吸附金

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农民再教育:社会主义新农村建设的推动力

继续教育研究 2009年第2期 33-35页

作者：沈辉周西安赵梅庆江苏大学京江学院江苏镇江212013

农村人口是我国人口的主体,农民问题是"三农"问题的核心,农民的再教育问题关系到社会主义新农村建设的目标能否顺利实现。完全认识当前农民再教育的必要性、难点和重点,对塑造新农民、普及新文化、建设社会主义新农村具有一定的现实意义。

关键词：农村再教育社会主义新农村