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Single-atom electrocatalysis: a new approach to in vivo electrochemical biosensing

Science China Chemistry 2019年第12期62卷 1720-1724页

作者： Hanfeng Hou Junjie Mao Yunhu Han Fei Wu meining zhang Dingsheng Wang Lanqun Mao Yadong Li Department of Chemistry Renmin University of ChinaBeijing 100872China Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living BiosystemsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China Department of Chemistry Tsinghua UniversityBeijing 100084China Key Laboratory of Functional Molecular Solids Ministry of EducationAnhui Key Laboratory of Molecule-Based MaterialsCollege of Chemistry and Materials ScienceAnhui Normal UniversityWuhu 241000China

Modulation of interfacial electron transfer has been proven to pave a new approach to in vivo electrochemical monitoring of brain chemistry;however,designing and establishing highly efficient electrocatalytic scheme towards neurochemicals remain a longstanding ***,we find that recently established single-atom catalyst(SAC)can be used for catalyzing the electrochemical process of physiologically relevant chemicals and thus offers a new avenue to in vivo electrochemical *** prove this new concept,we used Co single-atom catalyst(Co-SAC),in which the atomic active sites are dispersed in ordered porous N-doping carbon matrix at atomic level,as an example of SACs for analyzing glucose as the physiologically relevant model *** found that Co-SAC catalyzes the electrochemical oxidation of hydrogen peroxide(H2O2)at a low potential of ca.+0.05 V(***/AgCl).This property was further used for developing an oxidase-based glucose biosensor that was used subsequently as a selective detector of an online electrochemical system(OECS)for continuous monitoring of microdialysate glucose in rat *** OECS with Co-SAC-based glucose biosensor as the online detector was well responsive to glucose without interference from other electroactive species in brain *** study essentially offers a new approach to in vivo electrochemical analysis with SACs as electrocatalysts to modulate interfacial electron transfer.

关键词： single-atom catalyst in vivo analysis electrocatalysis biosensor

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Decreasing Pin1 suppressed the properties of migratory and invasive in colorectal carcinoma SW620 cells

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The Chinese-German Journal of Clinical Oncology 2010年第4期9卷 216-220页

作者： Liyuan Qin Hua Hao meining Li Dong zhang Jianlin zhang Niuliang Cheng Department of Biochemistry and Molecular Biology Shanxi Medical University Taiyuan 030001 China

Objective:The aim of our study was to investigate the effect of Pin1 on the expression of MMP-2 and MMP-9 in human colorectal carcinoma SW620 cells. Methods: We constructed a eukaryotic expression vector of RNA interfering (shRNA) for Pin1 gene (pGenesil-1-Pin1), and then observed its expression in SW620 cells by Western blotting. The cells motility were tested by wound healing assay and Boyden chamber assay. The protein levels and activity of MMP-2 and MMP-9 were tested by Western blotting and Gelatin zymography in SW620 cells after transfected with pGenesil-1-PIN1. Results: pGenesil-1-PIN1 was successfully constructed, which was confirmed by sequencing. Silencing the Pin1 by RNAi significantly decreased the cells motility from 96.4±3.9 per field (×10 objective) to 52.7±4.4 per field (P<0.05, Student's t-test) for SW620 cells transfected with pGenesil-1-PIN1 (SW620/p-shRNA) in Boyden chamber assay, and reduced the MMP-2 and MMP-9 expressions and activity in SW620 cells. The protein relative levels of MMP-2 were 0.32±0.04 for SW620/p-shRNA, and 0.76±0.03 for SW620/p-Con; MMP-9 were 0.41±0.09 for SW620/p-shRNA, and 0.94±0.07 for SW620/p-Con (p<0.05). Conclusion: Inhibited Pin1 expression may contribute to the suppression of the invasive and metastatic capacity of colon cancer cells in vitro.

关键词： colorectal cancer RNAi Pin1 MMPs metastasis and invasion

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Pin1 expression affects cell proliferation and apoptosis of SW620 cells in colorectal carcinoma

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The Chinese-German Journal of Clinical Oncology 2011年第2期10卷 100-104页

作者： Yan Zhu Liyuan Qin meining Li Dong zhang Yuehong zhang Niuliang Cheng Department of Biochemistry and Molecular Biology Shanxi Medical University Taiyuan 030001 China

Objective:The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal ***:We constructed a plasmid of RNA interfering(shRNA) for Pin1 gene(pGenesl-1-Pin1),then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome *** protein expression of Pin1 was tested by Western *** proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow *** order to explain further the effect of Pin1 in SW620 cells,the protein level of Bcl-2 was analyzed by Western ***:pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by *** protein relative levels of Pin1 were 0.06 ± 0.04 for the P-shRNA/SW620 cells,and 0.32 ± 0.09 for the P-Con/SW620 *** cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid,and the apoptotic rate was 12.38% ± 1.55% for the P-shRNA/SW620 *** the same time,we found that the protein expression of Bcl-2 was also *** results were 0.13 ± 0.04 for the P-shRNA/SW620 cells,and 0.36 ± 0.08 for the P-Con/SW620 ***:Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro.

关键词： RNAi Pin1 colorectal carcinoma(CRC) proliferation apoptosis

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NEUROELECTROANALYTICAL CHEMISTRY

NEUROELECTROANALYTICAL CHEMISTRY

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第十二届北京分析测试学术报告会暨展览会(BCEIA 2007)

作者： Kun LIU Yuqing LIN Ling XIANG meining zhang Lanqun MAO Beijing National Laboratory for Molecular Sciences Institute of Chemistry the Chinese Academy of Sciences (CAS)

Understanding of chemical essence in physiological and pathological events has been a long-standing *** becomes widely accepted that knowledge of the change in the level of chemical species in cerebral extracellular f...

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山地城市步行系统规划设计——以重庆渝中半岛为例

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规划师 2016年第5期32卷 136-143页

作者：韩列松余军张妹凝王梅重庆市规划设计研究院

随着人们环保意识的增强及生活观念的改变,步行交通越来越受到人们的青睐。而山地城市因高低起伏的地形,步行更是市民出行的主要方式之一。但是,其特殊的地形条件在为步行人群提供多样的景观和丰富的步行体验的同时,对步行系统的构建造... 详细信息

随着人们环保意识的增强及生活观念的改变,步行交通越来越受到人们的青睐。而山地城市因高低起伏的地形,步行更是市民出行的主要方式之一。但是,其特殊的地形条件在为步行人群提供多样的景观和丰富的步行体验的同时,对步行系统的构建造成了一定的阻碍(如山体隔绝、地形起伏等)。基于此,文章以重庆渝中半岛为例,分析其特殊的地形条件,确立了无缝对接城市公共交通、串联各类城市"吸引元"、增强步行系统设计指引的策略,以期构建"安全、公平、便捷、连续、舒适、优美"的步行交通系统,引导人们建立绿色健康的出行方式。

关键词：山地城市步行系统规划渝中半岛

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基于电位法的活体原位电化学分析研究进展

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延安大学学报（自然科学版） 2022年第3期41卷 24-33页

作者：刘冉彤张美宁中国人民大学理学院化学系北京100872 延安大学石油工程与环境工程学院陕西延安716000

活体层次探究脑神经化学物质有助于理解人类认知、情感等复杂生理过程的本质以及神经系统疾病的形成和发展规律,对预防和治疗神经系统疾病具有重要意义。电化学方法具有灵敏度高、选择性好、时空分辨高等优势,且检测电极较易微型化,在... 详细信息

活体层次探究脑神经化学物质有助于理解人类认知、情感等复杂生理过程的本质以及神经系统疾病的形成和发展规律,对预防和治疗神经系统疾病具有重要意义。电化学方法具有灵敏度高、选择性好、时空分辨高等优势,且检测电极较易微型化,在活体原位分析测定中得到广泛关注和应用。电位法不需要额外施加电位,能在接近生理情况下实现物质的检测,避免引起氧化应激反应,在活体原位电化学分析中逐渐引起青睐。文章从神经化学物质的不同类型入手,分别综述了电位法在离子等非电化学活性物质以及在具有电活性的神经化学物质分析检测中的基本原理及研究进展。

关键词：电位法活体分析离子选择性电极原电池型氧化还原电位分析法

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雷火灸对气虚证肺癌化疗病人疲乏及睡眠质量的影响

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护理研究 2021年第5期35卷 892-896页

作者：杨恒卢幻真张妹宁李黎丁利萍于嘉翔广西中医药大学附属瑞康医院广西530011

目的:探究雷火灸对气虚证肺癌化疗病人的疲乏及睡眠质量的影响。方法:将南宁市某三级甲等医院肿瘤科气虚证肺癌化疗住院病人84例随机分为观察组和对照组各42例,对照组采用常规护理,观察组在常规护理的基础上加用雷火灸,在干预前、干预... 详细信息

目的:探究雷火灸对气虚证肺癌化疗病人的疲乏及睡眠质量的影响。方法:将南宁市某三级甲等医院肿瘤科气虚证肺癌化疗住院病人84例随机分为观察组和对照组各42例,对照组采用常规护理,观察组在常规护理的基础上加用雷火灸,在干预前、干预完成时和干预后2周比较两组Piper疲乏修订量表评分、匹兹堡睡眠质量指数及安全性。结果:两组疲乏、睡眠质量总得分及各维度得分的组间效应、时间效应及交互效应均具有统计学意义(P<0.05)。结论:雷火灸应用于气虚证肺癌化疗病人可改善疲乏状况,提高睡眠质量,且较安全。

关键词：雷火灸肺癌化疗癌因性疲乏睡眠质量中医护理

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Synthesis Study toward Mayamycin

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Chinese Journal of Chemistry 2013年第1期31卷 93-99页

作者： Kui Wu meining Wang Qizheng Yao Ao zhang Department of Medicinal Chemistry China Pharmaceutical University Nanjing Jiangsu 210009 China Synthetic Organic & Medicinal Chemistry Laboratory Shanghai Institute ofMateria Medica Chinese Academy of Sciences Shanghai 201203 China

Natural product mayamycin is the first example in the angucycline class featuring a C-glycoside linkage at the C5-position of the benz[a]anthracenone core with remarkable biological activities. We successfully synthesized the two retrosynthetic fragments, but found that the final C-glycosylation did not occur. Alternatively, an A-ring satu- rated aglycon was prepared, but the proposed C-glycosylation still did not proceed. Finally, a simplified substrate was used and the subsequent C-glycosylation went through smoothly, giving a two-ring less analogue of mayamy- cin.

关键词： natural product angucycline mayamycin total synthesis C-glycosilation

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Glucocorticoid receptor signaling in the brain and its involvement in cognitive function

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Neural Regeneration Research 2025年第9期20卷 2520-2537页

作者： Chonglin Su Taiqi Huang Meiyu zhang Yanyu zhang Yan Zeng Xingxing Chen Brain Science and Advanced Technology Institute Hubei Province Key Laboratory of Occupational Hazard Identification and ControlSchool of MedicineWuhan University of Science and TechnologyWuhanHubei ProvinceChina

The hypothalamic-pituitary-adrenal axis regulates the secretion of glucoco rticoids in response to environmental *** the brain,a nuclear receptor transcription fa ctor,the glucocorticoid recepto r,is an important component of the hypothalamicpituitary-a d renal axis's negative feedback loop and plays a key role in regulating cognitive equilibrium and *** glucoco rticoid receptor influences cognitive processes,including glutamate neurotransmission,calcium signaling,and the activation of brain-derived neurotrophic factor-mediated pathways,through a combination of genomic and non-genomic *** interactions within the central nervous system can alter the expression and activity of the glucocorticoid receptor,there by affecting the hypothalamic-pituitary-a d renal axis and stress-related cognitive *** appropriate level of glucocorticoid receptor expression can improve cognitive function,while excessive glucocorticoid receptors or long-term exposure to glucoco rticoids may lead to cognitive *** with cognitive impairment-associated diseases,such as Alzheimer's disease,aging,depression,Parkinson's disease,Huntington's disease,stroke,and addiction,often present with dysregulation of the hypothalamic-pituitary-adrenal axis and glucocorticoid receptor *** review provides a comprehensive overview of the functions of the glucoco rticoid receptor in the hypothalamic-pituitary-a d renal axis and cognitive *** emphasizes that appropriate glucocorticoid receptor signaling fa cilitates learning and memory,while its dysregulation can lead to cognitive *** provides clues about how glucocorticoid receptor signaling can be targeted to ove rcome cognitive disability-related disorders.

关键词： brain-derived neurotrophic factor calcium signaling glucocorticoid receptor glucocorticoid glutamate transmission hypothalamic-pituitary-adrenal axis long-term potentiation neurocognitive disorders neuroplasticity stress

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Alectinib: a novel second generation anaplastic lymphoma kinase(ALK) inhibitor for overcoming clinically-acquired resistance

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Acta Pharmaceutica Sinica B 2015年第1期5卷 34-37页

作者： Zilan Song meining Wang Ao zhang CAS Key Laboratory of Receptor Research Synthetic Organic & Medicinal Chemistry LaboratoryShanghai Institute of Materia Medica (SIMM) Chinese Academy of Sciences

The development of inhibitors for the tyrosine anaplastic lymphoma kinase (ALK) has advanced rapidly, driven by biology and medicinal chemistry. The first generation ALK inhibitor crizotinib was granted US FDA approval with only four years of preclinical and clinical testing. Although this drug offers significant clinical benefit to the ALK-positive patients, resistance has been developed through a variety of mechanisms. In addition to ceritinib, alectinib is another second-generation ALK inhibitor launched in 2014 in Japan. This drug has a unique chemical structure bearing a 5H-benzo[b] carbazol-11(611)-one structural scaffold with an IC50 value of 1.9 nmol/L, and is highly potent against ALK bearing the gatekeeper mutation L1196M with an IC50 of 1.56 nmoL/L. In the clinic, alectinib is highly efficacious in treatment of ALK-positive non-small cell lung cancer (NSCLC), and retains potency to combat crizotinib-resistant ALK mutations L11.96.M, F1174L, R1275Q and C1156Y. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. All rights reserved.

关键词： Tyrosine anaplastic lymphoma kinase Alectinib Crizotinib Non-suaall cell lung cancer Resistance ALK mutations EML4-ALK

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