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Mechanism of interactions betweenα-conotoxin RegIIA and carbohydrates at the humanα3β4 nicotinic acetylcholine receptor

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Marine Life Science & Technology 2022年第1期4卷 98-105页

作者： meiling Zheng han-Shen Tae Liang Xue Tao Jiang Rilei Yu Molecular Synthesis Center&Key Laboratory of Marine Drugs Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao266003China Innovation Platform of Marine Drug Screening&Evaluation Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266100China Illawarra Health and Medical Research Institute(IHMRI) University of WollongongWollongongNSW2522Australia Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266003China

Conotoxins are marine peptide toxins from marine cone snails.Theα-conotoxin RegIIA can selectively act on human(h)α3β4 nicotinic acetylcholine receptor(nAChR),and is an important lead for drug development.The high-resolution cryo-electron microscopy structure of theα3β4 nAChR demonstrates several carbohydrates are located near the orthosteric binding sites,which may affectα-conotoxin binding.Oligosaccharide chains can modify the physical and chemical properties of proteins by changing the conformation,hydrophobicity,quality and size of the protein.The purpose of this study is to explore the effect of oligosaccharide chains on the binding modes and activities of RegIIA and its derivatives at hα3β4 nAChRs.Through computational simulations,we designed and synthesized RegIIA mutants at position 14 to explore the importance of residue H14 to the activity of the peptide.Molecular dynamics simulations suggest that the oligosaccharide chains affect the binding of RegIIA at the hα3β4 nAChR through direct interactions with H14 and by affecting the C-loop conformation of the binding sites.Electrophysiology studies on H14 analogues suggest that in addition to forming direct interactions with the carbohydrates,the residue might play an important role in maintaining the conformation of the peptide.Overall,this study further clarifies the structure–activity relationship ofα-conotoxin RegIIA at the hα3β4 nAChR and,also provides important experimental and theoretical basis for the development of new peptide drugs.

关键词： Oligosaccharide chains nAChR Conotoxin Action mechanism

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Effect of temperature and stress on high temperature creep behavior of Ni3Al-based single crystal superalloy

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Progress in Natural Science:Materials International 2022年第2期32卷 267-271页

作者： Cheng Chang Liwu Jiang meiling Wu Shusuo Li Yafang han National Center for Materials Service Safety University of Science and Technology Beijing Beijing Institute of Aeronautical Materials Research Institute of Aero-engine Beihang University

The creep behavior and dislocations mechanism of the Ni3Al-based single crystal alloy IC6 SX with [001] orientation were investigated under the testing conditions of 1100℃/137 MPa,1100℃/120 MPa and 1070℃/137 MPa.It was observed that the temperature and stress had a significant effect on the high temperature creep life of the single crystal alloy.As the temperature was reduced from 1100℃ to 1070 ℃,the creep life increased from65.07 h to 313.8 h.As the stress was reduced to 120 MPa,the creep life increased to 243.3 h.Under the high temperature and low stress condition the dislocations entered the γ’ phase by climbing caused by the atomic diffusion,instead of slipping.

关键词： Ni 3 Al-based Single crystal superalloy Creep properties Microstructure Dislocations configuration

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Creep behavior and dislocation mechanism of Ni3Al based single crystal alloy IC6SX at 760℃

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Progress in Natural Science:Materials International 2021年第5期31卷 755-761页

作者： Liwu Jiang Yanna Cui meiling Wu Shusuo Li Yafang han National Center for Materials Service Safety University of Science and Technology Beijing NCS Testing Technology Co. Ltd. Beijing Institute of Aeronautical Materials Research Institute of Aero-engine Beihang University

The creep behavior and dislocation mechanism of Ni3Al-based single crystal alloys IC6 SX with crystal orientation[001] which was prepared by seed crystal method under the testing conditions of 760℃/500 MPa,760℃/540 MPa and 760℃/580 MPa were investigated. The experimental results showed that the creep properties,dislocation morphology and mechanism of this alloy were different under different stress conditions. With the stress increasing from 500 MPa to 580 MPa, the creep life decreased from more than 1000 h to 32.64 h. The cubic degree of γ’phase in single crystal alloy decreased obviously and the size of γ’phase increased. The mechanism of dislocation movement also changed with the increasing stress. It was found that after the specimen was tested under the condition of 760℃/500 MPa the dislocation network prevented the movement of dislocations and it was difficult for dislocations to enter the γ’phase from the γ/γ’interface. When the stress was 540 MPa, the dislocations cut into the γ’phase by stacking fault. Furthermore, with the stress increasing to 580 MPa, the dislocation entered the γ’phase in the form of extended dislocation and Lomer-Cottrell dislocation.

关键词： Ni 3 Al-Based Single crystal alloy IC6SX Creep Dislocation mechanism

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In vitro Anthocyanin Induction and Metabolite Analysis in Malus spectabilis Leaves Under Low Nitrogen Conditions

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Horticultural Plant Journal 2020年第5期6卷 284-292页

作者： Jiaxin Meng Yan Gao Meiling Han Pengyuan Liu Chen Yang Ting Shen Houhua Li College of Landscape Architecture and Art Northwest A&F UniversityYanglingShaanxi 712100China

Anthocyanins are the most widely produced secondary metabolites in plants,and they play an important role in plant growth and reproduction.The nitrogen source is an important factor affecting anthocyanin production,but the nitrogen concentrations on metabolism and the underlying genetic basis remain unclear.In this study,in vitro anthocyanin induction was conducted on Malus spectabilis.The leaf explants were cultivated in media containing different nitrogen concentrations.The results suggested that when the nitrogen contents decreased in limit,the color of leaf explants turned from green to red,and increased anthocyanin accumulation led to a change in phenotype.Furthermore,the content of other flavonoids,such as dihydroquercetin,epicatechin,and catechin,increased under low nitrogen conditions.The transcription levels of the general flavonoid pathway genes,from phenylalanine ammonia lyase(PAL)to anthocyanidin synthase(ANS),were associated with the concentration of corresponding flavonoid compounds and phenotype changes.In particular,the expression level of ANS increased substantially under a low nitrogen treatment,which was significantly and positively correlated with the anthocyanin levels(R2=0.72,P<0.05).The increased expression patterns of anthocyanin pathway genes were similar to that of the transcription factor MYB10.We further verified MYB10 played an important role in the anthocyanin pathway in leaves of Malus spectabilis.These results suggested that we can improve the desirable ornamental plant phenotypes by controlling nitrogen content.This process may offer clues to further development of new agricultural practices.

关键词： crabapple pigment flavonoid in vitro low nitrogen stress

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Phenolic Profile, Antioxidant Activity and Anti-proliferative Activity of Crabapple Fruits

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Horticultural Plant Journal 2019年第4期5卷 155-163页

作者： Meiling Han Guo Li Xiaowei Liu Ai Li Peiqi Mao Pengyuan Liu Houhua Li College of Landscape Architecture and Art Northwest A&F UniversityYanglingShaanxi712100China Faculty of Natural Sciences Institute of Horticultural Production SystemsFloricultureLeibniz University HannoverHannover D-30419Germany Novogene Bioinformatics Technology Co.Ltd Beijing100089China

To explore the potential of crabapples as functional food, polyphenols in crabapples and ‘Fuji’ apples were extracted, and the phenolic profile,total polyphenols, antioxidant activity and anti-proliferative activity against several human cancer cells were determined. The results indicated that crabapple extracts have more abundant phenols and higher total polyphenols(from 4.46 to 46.63 mg GAE·g-1 DW) compared to ‘Fuji’ apples.Crabapple extracts possessed higher antioxidant activity than apple by DPPH and ABTS analysis. All fruit extracts exhibited inhibitory effects on proliferation in different cancer cells;however, crabapple extracts performed significantly better, with half inhibitory concentration(IC50)values varied from 48.34 μg·m L-1 to 974.81 μg·m L-1 for colon cancer cells SW480, 64.67–1 466.35 μg·m L-1 for stomach cancer cells BGC-803,78.88–910.64 μg·m L-1 for esophageal cancer cells CaEs-17. Besides, the red crabapples had higher antioxidant activity and anti-proliferative activity than yellow fruits. These results showed that crabapples, especially red crabapples, have great potential as a healthy food, as they are rich in phenolic compounds with high antioxidant and anti-proliferative activities to cancer cells.

关键词： Crabapple Phenolic profile Antioxidant activity Anti-proliferative activity Human cancer cell

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自信自强的基础工程:新时代公民道德自信的培育

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教育学术月刊 2023年第6期 98-105页

作者：钟美玲韩桥生江西中医药大学马克思主义学院南昌330004 江西师范大学马克思主义学院南昌330022

公民道德自信是中华民族实现伟大复兴的心理基础,培育公民道德自信是中华民族自信自强的基础工程。公民道德自信是公民对本国道德文化的认同、道德现状的肯定和道德未来发展的信心,是公民发自内心的在道德层面的自我肯定与相信。公民道... 详细信息

公民道德自信是中华民族实现伟大复兴的心理基础,培育公民道德自信是中华民族自信自强的基础工程。公民道德自信是公民对本国道德文化的认同、道德现状的肯定和道德未来发展的信心,是公民发自内心的在道德层面的自我肯定与相信。公民道德自信问题是一定历史语境的产物,国家的发展历程深深地影响着公民道德自信的程度。国家综合国力的提升、人民生活水平的提高和公民文明素养的进步是新时代公民道德自信培育的最大“底气”。公民道德自信的培育是一项系统工程,需要夯实公民道德自信培育的依托资源,强化公民道德自信培育的体系支撑,构建公民道德自信培育的力量系统,完善公民道德自信培育的实践机制。

关键词：公民道德道德自信培育新时代

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Characterization and Expression of Ammonium Transporter in Peach (Prunus persica) and Regulation Analysis in Response to External Ammonium Supply

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Phyton-International Journal of Experimental Botany 2020年第4期89卷 925-941页

作者： meiling Tang Yuhe Li Yahui Chen Lei han Hongxia Zhang Zhizhong Song College of Agriculture Ludong UniversityYantai264025China Yantai Academy of Agricultural Sciences Yantai264000China Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong(Ludong University) Yantai264025China Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education of China College of ForestNanjing Forest UniversityNanjing214008China

As the preferred nitrogen(N)source,ammonium(NH_(4)^(+))contributes to plant growth and development and fruit quality.In plants,NH 4+uptake is facilitated by a family of NH_(4)^(+) transporters(AMT).However,the molecular mechanisms and functional characteristics of the AMT genes in peach have not been mentioned yet.In this present study,excess NH_(4)^(+) stress severely hindered shoot growth and root elongation,accompanied with reduced mineral accumulation,decreased leaf chlorophyll concentration,and stunned photosynthetic performance.In addition,we identified 14 putative AMT genes in peach(PpeAMT).Expression analysis showed that PpeAMT genes were differently expressed in peach leaves,stems and roots,and were distinctly regulated by external NH_(4)^(+) supplies.Putative cis-elements involved in abiotic stress adaption,Ca^(2+) response,light and circadian rhythms regulation,and seed development were observed in the promoters of the PpeAMT family genes.Phosphorylation analysis of residues within the C-terminal of PpeAMT proteins revealed many conserved phosphorylation residues in both the AMT1 and AMT2 subfamily members,which could potentially play roles in controlling the NH 4+transport activities.This study provides gene resources to study the biological function of AMT proteins in peach,and reveals molecular basis for NH_(4)^(+) uptake and N nutrition mechanisms of fruit trees.

关键词： Prunus persica AMT transporters regulation by NH_(4)^(+)supply cis-elements phosphorylation site analysis

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某MW级风电变桨轴承边界条件仿真分析方法探讨

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哈尔滨轴承 2022年第4期43卷 19-21,37页

作者：韩美玲瓦轴集团有限责任公司工程中心辽宁瓦房店116300

风电变桨轴承是连接桨叶和轮毂的转盘轴承,是风力发电机组中技术含量较高的部件之一。由于风力发电机使用环境恶劣,维修成本高,所以变桨轴承要满足长寿命的使用要求,当前普遍采用有限元方法对变桨轴承进行结构整体分析与局部优化。本文... 详细信息

风电变桨轴承是连接桨叶和轮毂的转盘轴承,是风力发电机组中技术含量较高的部件之一。由于风力发电机使用环境恶劣,维修成本高,所以变桨轴承要满足长寿命的使用要求,当前普遍采用有限元方法对变桨轴承进行结构整体分析与局部优化。本文主要研究如何根据客户给定工况,对于大兆瓦级风电机组变桨轴承制定更合理的仿真分析方法,进一步提高变桨轴承仿真分析的效率和可靠性。

关键词：变桨轴承大兆瓦级有限元分析法高效性

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Potassium nutrition of maize:Uptake,transport,utilization,and role in stress tolerance

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The Crop Journal 2023年第4期11卷 1048-1058页

作者： meiling Zhang Yingying Hu Wu han Jian Chen Jinsheng Lai Yi Wang State Key Laboratory of Plant Environmental Resilience(SKLPER) College of Biological SciencesChina Agricultural UniversityBeijing 100193China National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China

Potassium(K) is an essential macronutrient for plant growth and development and influences yield and quality of agricultural crops.Maize(Zea mays) is one of the most widely distributed crops worldwide.In China,although maize consumes a large amount of K fertilizer,the K uptake/utilization efficiency(KUE)of maize cultivars is relatively low.Elucidation of KUE mechanisms and development of maize cultivars with higher KUE are needed.Maize KUE is determined by K+uptake,transport,and remobilization,which depend on a variety of K+channels and transporters.We review basic information about K+channels and transporters in maize,their functions and regulation,and the roles of K+in nitrogen transport,sugar transport,and salt tolerance.We discuss challenges and prospects for maize KUE improvement.

关键词： Maize Potassium Channel Transporter K uptake/utilization efficiency

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Copper‑Based Catalysts for Electrochemical Carbon Dioxide Reduction to Multicarbon Products

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Electrochemical Energy Reviews 2022年第3期5卷 178-212页

作者： Fangfang Chang meiling Xiao Ruifang Miao Yongpeng Liu Mengyun Ren Zhichao Jia Dandan han Yang Yuan Zhengyu Bai Lin Yang Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals Key Laboratory of Green Chemical Media and ReactionsMinistry of EducationSchool of Chemistry and Chemical EngineeringHenan Normal UniversityXinxiang 453007HenanChina Department of Chemical Engineering University of WaterlooWaterlooON N2L 3G1Canada

Electrochemical conversion of carbon dioxide into fuel and chemicals with added value represents an appealing approach to reduce the greenhouse effect and realize a carbon-neutral cycle,which has great potential in mitigating global warming and effectively storing renewable energy.The electrochemical CO_(2) reduction reaction(CO_(2)RR)usually involves multiproton coupling and multielectron transfer in aqueous electrolytes to form multicarbon products(C_(2+) products),but it competes with the hydrogen evolution reaction(HER),which results in intrinsically sluggish kinetics and a complex reaction mechanism and places higher requirements on the design of catalysts.In this review,the advantages of electrochemical CO_(2) reduction are briefly introduced,and then,different categories of Cu-based catalysts,including monometallic Cu catalysts,bimetallic catalysts,metal-organic frameworks(MOFs)along with MOF-derived catalysts and other catalysts,are summarized in terms of their synthesis method and conversion of CO_(2) to C2+products in aqueous solution.The catalytic mechanisms of these catalysts are subsequently discussed for rational design of more efficient catalysts.In response to the mechanisms,several material strategies to enhance the catalytic behaviors are proposed,including surface facet engineering,interface engineering,utilization of strong metal-support interactions and surface modification.Based on the above strategies,challenges and prospects are proposed for the future development of CO_(2)RR catalysts for industrial applications.

关键词： C_(2)products Carbon dioxide Nanocatalysts Electrochemical reduction Mechanism

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