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New developments in Biotechnology-an overview

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食品科学 2002年第11期23卷 156-159页

作者： J.W.vanderKamp meiwang InternationalAssociationforCerealScienceandTechnologyandTNONutritionandFoodResearch ZeistNetherlands DepartmentofAppliedPlantSciences TNOLeidenNetherlands

人类运用生物技术已经几千年了。直到上个世纪,发酵方法生产啤酒、白酒、面包、酱油以及其它食品都是在农产品领域的主要应用。自从50年前DNA和RNA结构和作用被揭示以来,一场生物技术的革命便产生了,并导致了两项关键技术的发展:1）通... 详细信息

人类运用生物技术已经几千年了。直到上个世纪,发酵方法生产啤酒、白酒、面包、酱油以及其它食品都是在农产品领域的主要应用。自从50年前DNA和RNA结构和作用被揭示以来,一场生物技术的革命便产生了,并导致了两项关键技术的发展:1）通过基因技术进行遣传因素的修改;2）新型快速多样分析工具;最新的发展都基于遗传学。这篇文章我将探讨以下问题:遗传因子修改的进展和公众认可的争论;基因学技术在农产品领域的应用;我的TNO研究所与中国伙伴联合的生物技术项目的例子;谷物科技方面的生物技术。

关键词：生物技术基因学技术遗传因子修改应用农产品

The Analysis of Pedigree GZ（Guangzhou）.1 with Primary Open Angle Glaucoma

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眼科学报 2002年第1期18卷 53-55页

作者： YehongZhuo meiwang ZhongshanOphthalmicCenter SunYet－senUniversityofMedicalSciencesGuangzhou510060China SunYet－senHospital SunYet－senUniversityofMedicalSciencesGuangzhou510060China

Purpose:To analyze the hereditary modality of primary open-angle glaucoma in China.Methods:The genetic form of Pedigree GZ.1 was analyzed using Mandalian hereditary rules.Results:Pedigree GZ.1 had following characteristics:1)The pedigree had four gene-rations,and there existed POAG patients in each generation;2) Each patient had a parent with POAG.If the parents didn't suffer from the disease,their children would not.3)The incidence of POAG in the relatives of the patients was 1/2 .In addition,The age of onset,intraocular pressure,fundus and prognosis was different from each other in the patients.Conclusions:1)Pedigree GZ.1 is inherited as an autosomal dominant trait.2) There exists individual differences of clinical manifestations in POAG patients.Eye Science 2000;16:53-55.

关键词：原发性开角型青光眼广州市家系分析连锁遗传

Pulse radiolysis study on aqueous solution of nicotine

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Nuclear Science and Techniques 2004年第1期15卷 35-38页

作者： WANGShi-Long meiwang NIYa-Ming YAOSi-De, WANGWen-Feng AnalysisandResearchCentreofTongjiUniversityShanghai200092 ShanghaiInstituteofAppliedPhysics theChineseAcademyofSciencesShanghai201800

Nicotine has been studied for the first time by pulse radiolysis techniques. It has been found that hy- drated electrons, hydrogen radicals and hydroxyl radicals can react with nicotine to produce anion radicals and n... 详细信息

关键词：脉冲射解烟碱直线加速器 N-甲-2-5-吡咯烷酮

Effects of Soil Texture on the Growth of Young Apple Trees and Soil Microbial Community Structure Under Replanted Conditions

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Horticultural Plant Journal 2020年第3期6卷 123-131页

作者： Yuefan Sheng Haiyan Wang meiwang Hanhao Li Li Xiang Fengbing Pan Xuesen Chen Xiang Shen Chengmiao Yin Zhiquan Mao College of Horticultural Science and Engineering/State Key Laboratory of Crop Biology Shandong Agricultural UniversityTai’anShandong 271018China College of Resources and Environment Shandong Agricultural UniversityNational Engineering Laboratory for Efficient Utilization of Soil and Fertilizer ResourcesTai’anShandong 271018China

A two-year field experiment was carried out in order to study the occurrence degree and mechanism of apple replant disease(ARD)in the apple orchards with different soil textures.So we can adopt appropriate controlmeasures according to the severity of ARD.Healthy two-year-old seedlings with consistent growth were selected,of which the root stock was T337 and the scion was Yanfu 3.There were significant differences in biomass between methyl bromide fumigation and replanted treatments,and the difference was the largest in clay loam,followed by sandy loam,and loam,which verified ARD in clay loam was most serious,followed by sandy loam and loam.Based on high-throughput sequencing of fungi in soil samples,fungal richness and diversity were the highest in clay loam,followed by sandy loam,and loam.The relative abundance of Fusarium in SX,SL,FX,FL,WX and WL was 7.33%,19.32%,2.70%,4.24%,10.71%and 23.87%,respectively.Based on Real-time quantitative analysis,there were significant differences in the number of Fusarium oxysporum and Fusarium solani between methyl bromide fumigation and replanted treatments,i.e.,clay loam>sandy loam>loam.Fusarium was the main pathogen causing ARD.This shows that ARD is the most serious under replanted clay loam condition.High-throughput sequencing technology was used to prove the difference in Fusarium was one of the important reasons for ARD under different soil textures.This technology provides a new idea for the prevention and control of ARD.

关键词： Apple Apple replant disease Fungi community structure Soil texture High-throughput sequencing

转录组和蛋白组联合揭示甜瓜响应涝害胁迫的分子机制

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核农学报 2024年第4期38卷 662-673页

作者：张焕欣闫承璞李国权张新龙曹娜乐美旺朱方红刘文革江西省农业科学院园艺研究所江西南昌330200 中国农业科学院郑州果树研究所河南郑州450009

为了探析甜瓜(Cucumis melo L.)响应涝害胁迫的分子机制,采用转录组和蛋白组学方法研究了甜瓜耐涝型材料L45和涝敏感型材料L39在正常培养和涝害胁迫处理条件下的差异表达基因和蛋白。结果表明,L39-W vs L39-C的差异表达基因数为3532个,... 详细信息

为了探析甜瓜(Cucumis melo L.)响应涝害胁迫的分子机制,采用转录组和蛋白组学方法研究了甜瓜耐涝型材料L45和涝敏感型材料L39在正常培养和涝害胁迫处理条件下的差异表达基因和蛋白。结果表明,L39-W vs L39-C的差异表达基因数为3532个,差异表达蛋白数为105个;L45-W vs L45-C的差异表达基因数为1842个,差异表达蛋白数为38个。转录组与蛋白组联合分析发现,L39-W vs L39-C中38个基因在mRNA和蛋白质水平上联合上调表达,12个基因联合下调表达;L45-W vs L45-C中7个基因联合上调表达,3个基因联合下调表达。KEGG显著性富集分析结果发现,L39-W vs L39-C中联合上调表达的基因显著富集在类黄酮生物合成、苯丙烷生物合成、苯丙氨酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成、精氨酸和脯氨酸代谢等;而L45-W vs L45-C中联合上调表达的基因显著富集在α-亚麻酸代谢、类黄酮生物合成、苯丙烷生物合成、亚油酸代谢等。本研究联合转录组与蛋白组数据进行了差异表达基因与蛋白分析,为进一步挖掘甜瓜响应涝害胁迫的关键基因及通路奠定了一定基础。

关键词：甜瓜涝害胁迫转录组蛋白组

SMOOTHING BY CONVEX QUADRATIC PROGRAMMING

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Journal of Computational Mathematics 2005年第2期23卷 211-216页

作者： Bing-shengHe Yu-meiwang DepartmentofMathematics NanjingUniversityNanjing210093China

In this paper, we study the relaxed smoothing problems with general closed convex constraints. It is pointed out that such problems can be converted to a convex quadratic minimization problem for which there are good ... 详细信息

关键词： Relaxed smoothing Convex quadratic Programming

A Real-world Prospective Study of Mother-to-child Transmission of HBV in China Using a Mobile Health Application (Shield 01)

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Journal of Clinical and Translational Hepatology 2020年第1期8卷 1-8页

作者： Xueru Yin Guorong Han Hua Zhang meiwang Wenjun Zhang Yunfei Gao Mei Zhong Xiaolan Wang Xiaozhu Zhong Guojun Shen Chuangguo Yang Huiyuan Liu Zhihong Liu Po-Lin Chan Marc Bulterys Fuqiang Cui Hui Zhuang Zhihua Liu Jinlin Hou Department of Infectious Diseases Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongChina Department of Gynecology and Obstetrics The Second Affiliated Hospital of the Southeast UniversityNanjingJiangsuChina Department of Obstetrics and Gynecology Beijing Youan HospitalCapital Medical UniversityBeijingChina Department of Obstetrics and Gynecology The Fifth Medical CentreChinese People’s Liberation Army General HospitalBeijingChina Department of Obstetrics and Gynecology The Fifth Affiliated Hospital of Southern Medical UniversityGuangzhouGuangdongChina Department of Obstetrics and Gynecology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongChina Department of Obstetrics Jiujiang Maternal and Child Care CenterJiujiangJiangxiChina Department of Infectious Diseases Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdongChina Department of Infectious Diseases Third People’s Hospital of JiujiangJiujiangJiangxiChina Department of Infectious Diseases The Third Affiliated Hospital of Southern Medical universityGuangzhouGuangdongChina Department of Severe Liver Diseases Guangzhou Eighth People’s HospitalGuangzhouGuangdongChina World Health Organization/Western Pacific Regional Office(WPRO) ManilaPhilippines Global Hepatitis Programme World Health OrganizationGenevaSwitzerland School of Public Health Peking UniversityBeijingChina Department of Microbiology and Center of Infectious Diseases School of Basic Medical SciencesPeking University Health Science CenterBeijingChina

Background and Aims:The World Health Organization(WHO)Western Pacific Region set a target of eliminating mother-to-child transmission(MTCT)of hepatitis B virus(HBV)by 2030.To assess the feasibility of this target in China,we carried out an epidemiological study to investigate the status quo of MTCT in the real-world setting.Methods:One thousand and eight hepatitis B surface antigen-positive preg-nant women were enrolled at 10 hospitals.Immunoprophy-laxis was administered to infants.In addition,mothers with HBV DNA level>2,000,000 IU/mL were advised to initiate antiviral therapy during late pregnancy.A health application called SHIELD was used to manage the study.Results:Nine hundred and five of the enrolled mothers,with 924 infants,completed the follow-up.Birth-dose hepatitis B vaccine and hepatitis B immunoglobulin were received by 99.7%and 99.7%of infants,respectively,within 24 h after birth.There ;were 446 mothers who received antiviral therapy,including 72.3%of the mothers with HBV DNA level>2,000,000 IU/mL and 21.0%of the mothers with HBV DNA level<2,000,000 IU/mL.Eight infants were infected with HBV.The overall rate of MTCT was 0.9%.Birth defects were rare(0.5%among in-fants with maternal antiviral exposure versus 0.7%among infants without exposure;p=1.00).Conclusions:The MTCT rate was lower than the WHO Western Pacific Region elimina-tion MTCT target in this real-world study,indicating that a comprehensive management composed of immunoprophy-laxis to infants and antiviral prophylaxis to mothers may be a feasible strategy to achieve the 2030 WHO elimination goal.

关键词： Mother-to-child transmission Hepatitis B virus Antiviral therapy Immunoprophylaxis Shield Project

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0201封装元件批量组装过程质量鉴定

中国电子商情（空调与冷冻） 2003年第7期 24-28页

作者： meiwang DongkaiShangguan M.T.Ong FredrikMattsson DavidGeiger SammyYi KIC公司 Flextronics公司

Indomethacin suppresses growth of colon cancer via inhibition of angiogenesis in vivo

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World Journal of Gastroenterology 2005年第3期11卷 340-343页

作者： Hong-meiwang Gui-YingZhang DepartmentofGastroenterology XiangyaHospitalofCentralSouthUniversityChangsha410078HunanProvinceChina

AIM: It has been reported that regular consumption of nonsteroidal anti-inflammatory drugs like indomethacin decreases the incidence and mortality rate of a number of gastrointestinal cancers. We aimed to explore the efficacy and possible mechanisms of indomethacin on tumor growth and tumor angiogenesis of human colon cancer xenografts in nude mice,METHODS: MTT (thiazolyl blue) assay was used to assess the effect of indomethacin on cultured human colorectal cancer cell line HCT116. HCT116 cells were inoculated subcutaneously into BALB/c-nu/nu mice. After oral administration of indomethacin, 3 mg/kg·d for 4 wk, animals were sacrificed by cervical dislocation. Immunohistochemical staining was employed to determine the microvessel density (MVD) and vascular endothelial growth factor (VEGF)expression in tumor tissues.RESULTS: Indomethacin, a non-selective COX inhibitor,significantly decreased the viability of HCT116 cells in a dose-dependent manner (P＜0.05) with 50% inhibition at approximately 318.2±12.7 μmol/L. Growth of HCT116 cell tumor was significantly suppressed by indomethacin. The tumor volume was significantly decreased in the treated group (458.89±32.07 mm3) compared to the control group (828.21±31.59 mm3) (P＜0.05). The MVD of the treated group (19.50±5.32) was markedly decreased compared to the control group (37.40±4.93) (P＜0.001). The VEGF expression of the treated group (1.19±0.17) was obviously reduced as compared to the control group (1.90±0.48)(P＜0.01). The decrease in MVD was positively correlated with the decrease of VEGF expression (rs = 0.714, P＜0.05).We did not see gastrointestinal complications in the treated group and no differences were noted in the body weight of the mice between the two groups throughout the study (P＞0.05).CONCLUSION: Indomethacin can significantly decrease the viability of cultured HCT116 cells and retard human colorectal HCT116 cell tumor growth via inhibiting tumor angiogenesis, which might be through reduction of VEGF expression.

关键词：消炎痛生长抑制作用结肠癌肿瘤血管生成 VEGF 微脉管密度