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Science China Life Sciences 2024年

作者： Xiaoyu Wang Chuchu chen chenggan Li Xiaochang chen Rong Xu meilin chen Yongpeng Li Yihao Liu Xiaohong Liu Yaosheng chen Delin Mo State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-Sen University Shaanxi Basic and Clinical Translational Research Team for Atherosclerotic Cardiovascular Disease Shaanxi Key Laboratory of Ischemic Cardiovascular Disease Institute of Basic and Translational MedicineXi'an Medical University

Intramuscular fat (IMF) is a complex adipose tissue within skeletal muscle, appearing specially tissue heterogeneous, and the factors influencing its formation remain unclear. In conditions such as diabetes, aging, and muscle wasting, IMF was deposited in abnormal locations in skeletal muscle, damaged the normal physiological functions of skeletal muscle. Here, we used Longissimus dorsi muscles from pigs with different IMF contents as samples and adopted a method combining spatial transcriptome (ST) and single-nucleus RNA-seq to identify the spatial heterogeneity of IMF. ST revealed that genes involved in TGF-β signaling pathways were specifically highly enriched in IMF. In lean pigs, IMF autocrine produces more TGF-β2, while in obese pigs, IMF received more endothelial-derived TGF-β1. In vitro experiments have proven that porcine endothelial cells in a simulated high-fat environment released more TGF-β1 than TGF-β2. Moreover,under obesity mice, the addition of TGF-β after muscle injury abolished IMF production and slowed muscle repair, whereas TGF-β inhibition accelerated muscle repair. Our findings demonstrate that the TGF-β pathway specifically regulates these processes, suggesting it as a potential therapeutic target for managing muscle atrophy in obese patients and enhancing muscle repair while reducing IMF deposition.

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数字经济驱动了居民消费结构升级吗——基于调节效应和门槛效应的分析

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调研世界 2024年第6期 52-61页

作者：包振山陈美琳翁梅新潟大学(日本) 南京大学经济学院盐城师范学院商学院中韩(盐城)产业园创新发展研究院

本文基于2012—2021年我国省级面板数据,实证分析数字经济发展对居民消费结构升级的影响效应及作用机制。研究发现:数字经济发展能显著促进居民消费结构升级,且对东部和中部地区的促进作用更为明显;产业升级和进口贸易是数字经济影响居... 详细信息

本文基于2012—2021年我国省级面板数据,实证分析数字经济发展对居民消费结构升级的影响效应及作用机制。研究发现:数字经济发展能显著促进居民消费结构升级,且对东部和中部地区的促进作用更为明显;产业升级和进口贸易是数字经济影响居民消费结构的传导路径;居民人均可支配收入、城乡收入差距、教育水平、政策支持、通货膨胀是数字经济影响居民消费结构的重要作用机制。进一步分析表明,数字经济发展对居民消费结构升级的促进作用随产业升级呈“边际效应递增”,随进口贸易额的增加呈“边际效应递减”,且推动产业不断升级是数字经济优化居民消费结构的长效作用机制。据此提出推动居民消费结构升级的政策建议。

关键词：数字经济居民消费结构进口贸易产业升级

基于斑马鱼模型及网络药理学的晕痛定胶囊镇痛活性及作用机制探讨

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药物评价研究 2024年第2期47卷 261-276页

作者：陈祺陈林珍陈美琳王亚峰张雪于雪戴胜云马志强赵崇军北京中医药大学中药品质评价北京市重点实验室北京102488 北京中医药大学中医学院北京100029 中国食品药品检定研究院北京102629 世界中医药学会联合会北京100101

目的基于斑马鱼模型探究晕痛定胶囊的镇痛活性,采用网络药理学方法预测晕痛定胶囊的镇痛作用机制。方法采用佛波酯构建斑马鱼疼痛模型,以阿司匹林为阳性对照,利用行为轨迹分析系统监测斑马鱼的运动轨迹,以运动次数、运动时间、运动距离... 详细信息

目的基于斑马鱼模型探究晕痛定胶囊的镇痛活性,采用网络药理学方法预测晕痛定胶囊的镇痛作用机制。方法采用佛波酯构建斑马鱼疼痛模型,以阿司匹林为阳性对照,利用行为轨迹分析系统监测斑马鱼的运动轨迹,以运动次数、运动时间、运动距离、运动速度等运动状态为指标评价晕痛定胶囊及其组方药材的镇痛活性;同时运用网络药理学及分子对接技术预测晕痛定胶囊镇痛核心成分及潜在靶点;选取网络药理学预测的核心成分法卡林二醇和金色酰胺醇酯为实验药物,利用斑马鱼疼痛模型验证镇痛活性。结果斑马鱼疼痛模型镇痛活性评价实验中,晕痛定胶囊及其组方药材川芎、蜜环菌均能显著降低模型斑马鱼的快速运动和慢速运动(P<0.01),且晕痛定胶囊作用强于川芎和蜜环菌。网络药理学筛选得到晕痛定胶囊镇痛活性成分87个及其作用靶点159个,利用蛋白质-蛋白质相互作用(PPI)网络分析得到法卡林二醇和金色酰胺醇酯等核心成分和MAPK1等核心靶点,分子对接结果显示核心成分及核心靶点间具有较好的结合活性。法卡林二醇和金色酰胺醇酯镇痛活性实验结果表明单独给药法卡林二醇和金色酰胺醇酯可以达到良好的镇痛效果,与网络药理学预测的核心成分结果相符。结论晕痛定胶囊能够缓解暴露于佛波酯中斑马鱼的疼痛状态,具有较明显的镇痛作用;预测其作用机制可能与减少神经损伤及其炎症反应和促进细胞凋亡等过程有关;且法卡林二醇和金色酰胺醇酯为其发挥镇痛作用的核心成分。

关键词：晕痛定胶囊镇痛活性斑马鱼网络药理学分子对接法卡林二醇金色酰胺醇酯

Influence of freeze tube deviation on the development of frozen wall during long cross-passage construction

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Research in Cold and Arid Regions 2022年第4期14卷 250-257页

作者： JunHao chen JianLin Wang LeXiao Wang Han Li meilin chen School of Civil Engineering Fujian University of TechnologyFuzhouFujian350118China Key Laboratory of Underground Engineering Fujian Province UniversityFuzhouFujian350118China

This paper investigates the influence of the deviation in freeze pipe installation on the development of the frozen wall in long cross passages by numerical simulation with ANSYS *** study case is from the artificial ground freezing project along the Fuzhou Metro Line 2 between Ziyang Station and Wuliting *** freezepipe configurations,i.e.,one with perfectly aligned pipes without any deviation from design and another with randomly distributed deviation,are included for *** effect of the random deviation in the freeze pipes on frozen wall interconnection time,the thickness of the frozen wall and the development of the temperature field is *** the characteristic section of the numerical model at a depth of 25 m,it is found that the frozen wall interconnection time under the random deviation case and no deviation case is 24 days and 18 days,*** difference in the thickness of the thinnest frozen wall segment between the random deviation and no deviation cases is the largest in the early freezing stage(up to 0.75 m),which decreases with time to 0.31 m in the late freezing *** effects of random deviation are more significant in the early freezing stage and diminish as the freezing time increases.

关键词： Long cross passages Artificial ground freezing Random deflection Numerical simulation

Emerging roles of activating transcription factor (ATF) family members in tumourigenesis and immunity: Implications in cancer immunotherapy

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Genes & Diseases 2022年第4期9卷 981-999页

作者： meilin chen Yijun Liu Yuqin Yang Yanbing Qiu Zhicheng Wang Xiaoxu Li Wenling Zhang Department of Medical Laboratory Science The Third Xiangya HospitalCentral South UniversityChangshaHunan 410013PR China

Activating transcription factors, ATFs, are a group of bZIP transcription factors that act as homodimers or heterodimers with a range of other bZIP factors. In general, ATFs respond to extracellular signals, indicating their important roles in maintaining homeostasis. The ATF family includes ATF1, ATF2, ATF3, ATF4, ATF5, ATF6, and ATF7. Consistent with the diversity of cellular processes reported to be regulated by ATFs, the functions of ATFs are also diverse. ATFs play an important role in cell proliferation, apoptosis, differentiation and inflammation-related pathological processes. The expression and phosphorylation status of ATFs are also related to neurodegenerative diseases and polycystic kidney disease. Various miRNAs target ATFs to regulate cancer proliferation, apoptosis, autophagy, sensitivity and resistance to radiotherapy and chemotherapy. Moreover, ATFs are necessary to maintain cell redox homeostasis. Therefore, deepening our understanding of the regulation and function of ATFs will provide insights into the basic regulatory mechanisms that influence how cells integrate extracellular and intracellular signals into genomic responses through transcription factors. Under pathological conditions, especially in cancer biology and response to treatment, the characterization of ATF dysfunction is important for understanding how to therapeutically utilize ATF2 or other pathways controlled by transcription factors. In this review, we will demonstrate how ATF1, ATF2, ATF3, ATF4, ATF5, ATF6, and ATF7 function in promoting or suppressing cancer development and identify their roles in tumour immunotherapy.

关键词： Anti-tumor effect ATF bZIP Cancer Immunity Tumorigenesis

Dynamic Event-Triggered Consensus Control for Input Constrained Multi-Agent Systems With a Designable Minimum Inter-Event Time

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IEEE/CAA Journal of Automatica Sinica 2024年第3期11卷 649-660页

作者： meilin Li Yue Long Tieshan Li Hongjing Liang C.L.Philip chen the School of Automation Engineering University of Electronic Science and Technology of ChinaChengdu 611731China IEEE the Yangtze Delta Region Institute University of Electronic Science and Technology of ChinaHuzhou 313000 the Laboratory of Electromagnetic Space Cognition and Intelligent Control Beijing 100089China the School of Computer Science and Engineering South China University of TechnologyGuangzhou 510006

This paper investigates the consensus control of multi-agent systems(MASs) with constrained input using the dynamic event-triggered mechanism(ETM).Consider the MASs with small-scale networks where a centralized dynamic ETM with global information of the MASs is first ***,a distributed dynamic ETM which only uses local information is developed for the MASs with large-scale *** is shown that the semi-global consensus of the MASs can be achieved by the designed bounded control protocol where the Zeno phenomenon is eliminated by a designable minimum inter-event *** addition,it is easier to find a trade-off between the convergence rate and the minimum inter-event time by an adjustable ***,the results are extended to regional consensus of the MASs with the bounded control *** simulations show the effectiveness of the proposed approach.

关键词： Constrained input designable minimum inter-event time directed communication topology dynamic event-triggered mechanism MASs consensus control

基于滑膜控制的农用智能无人机自动导航控制系统

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自动化应用 2024年第15期65卷 28-30页

作者：石峰峥陈美琳王雨欣张泽中崔云昊河南工业大学电气工程学院河南郑州450000

针对农业环境的动态性和复杂性,提出了一种基于滑膜控制的农用无人机自动导航控制系统。首先,设计了一种面向动态环境的滑模控制器,确保了无人机面对外界干扰时的飞行稳定性;然后,引入强化学习算法对控制参数进行在线调整,提高了系统的... 详细信息

针对农业环境的动态性和复杂性,提出了一种基于滑膜控制的农用无人机自动导航控制系统。首先,设计了一种面向动态环境的滑模控制器,确保了无人机面对外界干扰时的飞行稳定性;然后,引入强化学习算法对控制参数进行在线调整,提高了系统的适应能力;最后,搭建了硬件在环仿真平台,并在实际农田环境下进行飞行测试。结果表明,该控制系统有效提升了农用智能无人机的自动导航性能,为智慧农业的发展提供了技术支撑。

关键词：农用无人机滑模控制强化学习

The interaction between polyphyllin I and SQLE protein induces hepatotoxicity through SREBP-2/HMGCR/SQLE/LSS pathway

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Journal of Pharmaceutical Analysis 2023年第1期13卷 39-54页

作者： Zhiqi Li Qiqi Fan meilin chen Ying Dong Farong Li Mingshuang Wang Yulin Gu Simin Guo Xianwen Ye Jiarui Wu Shengyun Dai Ruichao Lin Chongjun Zhao Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing100029China Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of ChinaShaanxi Normal UniversityXi'an710119China National Institutes for Food and Drug Control Beijing102629China

Polyphyllin I(PPI)and polyphyllin II(PII)are the main active substances in the Paris ***,liver toxicity of these compounds has impeded their clinical application and the potential hepatotoxicity mechanisms remain to be *** this work,we found that PPI and PII exposure could induce significant hepatotoxicity in human liver cell line L-02 and zebrafish in a dose-dependent *** results of the proteomic analysis in L-02 cells and transcriptome in zebrafish indicated that the hepatotoxicity of PPI and PII was associated with the cholesterol biosynthetic pathway disorders,which were alleviated by the cholesterol biosynthesis inhibitor ***,3-hydroxy-3-methy-lglutaryl CoA reductase(HMGCR)and squalene epoxidase(SQLE),the two rate-limiting enzymes in the cholesterol synthesis,selected as the potential targets,were confirmed by the molecular docking,the overexpression,and knockdown of HMGCR or SQLE with ***,the pull-down and surface plasmon resonance technology revealed that PPI could directly bind with SQLE but not with ***,these data demonstrated that PPI-induced hepatotoxicity resulted from the direct binding with SQLE protein and impaired the sterol-regulatory element binding protein 2/HMGCR/SQLE/lanosterol synthase pathways,thus disturbing the cholesterol biosynthesis *** findings of this research can contribute to a better understanding of the key role of SQLE as a potential target in drug-induced hepatotoxicity and provide a therapeutic strategy for the prevention of drug toxic effects with similar structures in the future.

关键词： Polyphyllin I Polyphyllin II Zebrafish Hepatotoxicity SQLE

Characteristics of genetic basis copy number variation in production and adaptation traits of Chinese indigenous sheep

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Journal of Genetics and Genomics 2024年第5期51卷 566-569页

作者： meilin Jin Gang Liu Jian Lu Zhiqiang chen Huihua Wang Taotao Li Caihong Wei State Key Laboratory of Animal Biotech Breeding Institute of Animal SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100193China College of Animal Science and Technology China Agricultural UniversityBeijing100193China National Animal Husbandry Service National Center of Preservation&Utilization of Animal Genetic ResourcesBeijing 100125China Glbizzia Biosciences Beijing 102629China

Copy number variations(CNVs)range in size from 50 base pairs(bp)to several megabases(Mb)and encompass various types of structural variations,such as insertions,deletions,duplications,and complex multiallelic events(MacDonald et al.,2014).These genomic alterations modulate phenotype by altering gene dosage and transcriptional regulation,and are key drivers of biological evolution and determinants of genetic diversity and phenotypic *** recent years,CNVs have been detected in the genomes of various livestock species(Yuan et al.,2021;chen et al.,2022).Upadhyay et al.(2017)conducted a comprehensive analysis of CNV distribution in the European cattle genome and determined that the Kit gene is associated with color-sidedness in English longhorn *** study presented extensive genome-wide information on CNVs in wild,commercial,and local chicken breeds to gain an integrated understanding of the domestication process and exclusively identified the genes involved(chen et al.,2022).

关键词： sided number dosage

公立医院窗口服务质量评价指标体系构建与实证研究

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中国医院 2024年第3期28卷 23-25页

作者：金建芳安妮周肖燕刘彤彤陈美林苏州大学附属第二医院江苏省苏州市215004 无锡市第九人民医院江苏省无锡市214000 东南大学附属中大医院江苏省南京市210009

目的:构建公立医院窗口服务质量评价指标体系并进行实证研究,为更好地评价公立医院窗口服务质量提供依据。方法:通过访谈法和文献研究法,梳理国家、省、市、区(县)级等相关政策文件,运用Nvivo12.0软件初步构建医院窗口服务质量指标体系... 详细信息

目的:构建公立医院窗口服务质量评价指标体系并进行实证研究,为更好地评价公立医院窗口服务质量提供依据。方法:通过访谈法和文献研究法,梳理国家、省、市、区(县)级等相关政策文件,运用Nvivo12.0软件初步构建医院窗口服务质量指标体系。采用德尔菲专家咨询法确定评价指标,评价指标权重利用层次分析法以及变异系数法进行计算,设计评分问卷;选取江苏省某公立医院9个窗口进行实证研究,从而完善该评价指标体系。结果:最终确定指标体系包含3项一级指标、10项二级指标和37项三级指标,并与窗口管理部门进行求证,掌握各窗口日常工作运行情况,与问卷调查结果基本相符。结论:构建的窗口服务质量评价指标体系专家意见协调性较好,评价指标及评估结果可信度较高;通过实证研究证实所建立的评价指标体系具有实践性,可靠性强。

关键词：公立医院窗口服务服务质量评价指标体系实证研究

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