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Effects of exogenous paclobutrazol and sampling time on the efficiency of in vitro embryo rescue in the breeding of new seedless grape varieties

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Journal of Integrative Agriculture 2022年第6期21卷 1633-1644页

作者： XU Teng-fei GUO yu-rui wang Wen-yuan yuAN Xiao-jian CHU Yan-nan wang Xiao-wei HAN yu-lei wang yue-jin SONG Rui FANG yu-lin wang Lu-jun XU Yan State Key Laboratory of Crop Stress Biology for Arid Areas College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China College of Plant Science Tibet Agricultural and Animal Husbandry UniversityNyingchi 860000P.R.China Institute for China Wine Industry Technology Yinchuan 750000P.R.China Institute for Weinan Grape Research Weinan 714099P.R.China

Embryo rescue technology plays an important role in seedless grape ***,the efficiency of embryo rescue,including the embryo formation,germination,and seedling rates,is closely related to the parental genotypes,degree of abortion,growth medium,and plant growth *** this study,we investigated the effects of different concentrations of paclobutrazol(PAC),a plant growth regulator,and embryo collection times on the embryo formation,germination,and seedling rates for different hybrid combinations of grape breeding varieties used for their aroma and cold-resistance *** results showed that the different PAC concentrations had varying impacts on the development of ovules and embryos from the different grape *** embryo formation rates of the‘Sultanina Rose’×‘Beibinghong’and‘Kunxiang Seedless’×‘Taishan-2’crosses were the highest under the 5.1μmol L^(-1)PAC *** 1.0μmol L^(-1)PAC treatment was optimal for the germination and seedling development of the‘Sultanina Rose’×‘Beibinghong’embryos,whereas the 0.2μmol L^(-1)PAC treatment induced the highest germination rate for the‘Sultanina Rose’×‘Kunxiang Seedless’*** optimal sampling times for each cross varied as 39 d after pollination(DAP)for the‘Flame Seedless’×‘Muscat Hamburg’cross,46 DAP for the‘Kunxiang Seedless’×‘Beibinghong’cross,and 41 DAP for the‘Ruby Seedless’×‘Beibinghong’and‘Fantasy Seedless’×‘Shuangyou’***,the medium modified with 0.5 g L^(-1)of indole-3-butyric acid allowed the malformed seedlings to develop into plantlets and achieve larger *** study provides a useful basis for further studies into grape embryo rescue and could improve breeding efforts for new seedless grape varieties.

关键词： Vitis grape embryo rescue paclobutrazol malformed seedlings

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Elucidating the interaction of kansui and licorice by comparative plasma/tissue metabolomics and a heatmap with relative fold change

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Journal of Pharmaceutical Analysis 2019年第5期9卷 312-323页

作者： Yan-Yan Chen Juan Shen yu-Ping Tang Jin-Gao yu Jing wang Shi-Jun yue Jie Yang Jia-Qian Chen Li-mei Feng Zhen-Hua Zhu Wei-Wei Tao Li Zhang Jin-Ao Duan Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation)Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Researchand Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources IndustrializationShaanxi University of Chinese MedicineXi’an 712046Shaanxi ProvinceChina Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicineand Jiangsu Key Laboratory for High Technology Research of TCM FormulaeNanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina

Although compatibility is highly advocated in traditional Chinese medicine (TCM), inappropriate combination of some herbs may reduce the therapeutic action and even produce toxic effects. Kansui and licorice, one of TCM “Eighteen Incompatible Medicaments”, are the most representative cases of improper herbal combination, which may still be applied simultaneously under given conditions. However, the potential mechanism of their compatibility and incompatibility is unclear. In the present study, two different ratios of kansui and licorice, representing their compatibility and incompatibility respectively, were designed to elucidate their interaction by comparative plasma/tissue metabolomics and a heatmap with relative fold change. As a result, glycocholic acid, prostaglandin F2a, dihydroceramide and sphinganine were screened out as the principal alternative biomarkers of compatibility group;sphinganine, dihydroceramide, arachidonic acid, leukotriene B4, acetoacetic acid and linoleic acid were those of incompatibility group. Based on the values of biomarkers in each tissue, the liver was identified as the compatible target organ, while the heart, liver, and kidney were the incompatible target organs. Furthermore, important pathways for compatibility and incompatibility were also constructed. These results help us to better understand and utilize the two herbs, and the study was the first to reveal some innate characters of herbs related to TCM “Eighteen Incompatible Medicaments”.

关键词： Comparative metabolomics Kansui Licorice Incompatibility Heatmap

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杨凌糖丝菌蛋白激发子HSyp1和HSyp2诱导植物抗病功能

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微生物学报 2025年第1期65卷 268-282页

作者：于洪佳张家阳王平平崔传斌颜霞黄丽丽西北农林科技大学生命科学学院陕西杨凌712100 作物抗逆与高效生产全国重点实验室陕西杨凌712100 中国烟草总公司陕西省烟草公司陕西西安710000 西北农林科技大学植物保护学院陕西杨凌712100

【目的】前期研究从杨凌糖丝菌(Saccharothrix yanglingensis)Hhs.015全基因组中初步筛选出4种能够诱导烟草发生过敏反应(hypersensitiveresponse)的蛋白激发子,本研究进一步探讨引发明显HR的2个激发子及其相关的免疫机理,为深入研究Hhs... 详细信息

【目的】前期研究从杨凌糖丝菌(Saccharothrix yanglingensis)Hhs.015全基因组中初步筛选出4种能够诱导烟草发生过敏反应(hypersensitiveresponse)的蛋白激发子,本研究进一步探讨引发明显HR的2个激发子及其相关的免疫机理,为深入研究Hhs.015诱导植物抗病性的分子机制提供依据。【方法】通过过敏反应和活性氧检测等方法筛选能够诱发植物免疫反应的蛋白激发子。使用原核表达系统纯化蛋白,通过观察病斑表型、测定防御相关酶活性及分析防御基因表达,验证这些激发子在诱导植物抗病性方面的功能。同时,将蛋白激发子应用于苗期拟南芥(Arabidopsis thaliana),以观察其促生效果。【结果】从Hhs.015中筛选出能够引起烟草(Nicotianabenthamiana)过敏反应、促进胼胝质和活性氧沉积、防御基因上调,且能够以可溶性形式表达的蛋白激发子HSyp1和HSyp2。植物抗病性检测结果表明,HSyp1和HSyp2能够提高不同植物对核盘菌(Sclerotinia sclerotiorum)、黑杆病菌(Phytophthora capsica)以及黑腐皮壳菌(Valsa mali)的抗病性。此外,研究还发现HSyp1和HSyp2对苗期拟南芥的子叶和根部发育具有一定的促生作用,其中HSyp2的促生效果比HSyp1更为显著。【结论】本研究筛选出2个蛋白激发子HSyp1和HSyp2,能够激发植物免疫反应,提高植物抗病能力,并具有促生作用。这些发现为开发新型生物农药提供了理论基础,也为其未来的田间应用提供了实验依据。

关键词：原核表达蛋白激发子过敏反应植物抗病性促生作用

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1973—2023年慢性心力衰竭中医证候特征演变规律文献研究

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世界科学技术-中医药现代化 2025年第1期27卷 121-131页

作者：张赤道王永霞朱明军邢作英邱伯雍王新陆于瑞河南中医药大学第一附属医院心脏中心河南中医药大学

目的通过文献挖掘1973-2023年慢性心力衰竭中医证候，总结其中医证候特征演变规律。方法检索1973年1月1日-2023年10月1日中国知网等3个数据库中有关慢性心力衰竭中医证候临床研究的相关文献，使用SPSS 21.0和Rstudio软件对最终纳入文... 详细信息

目的通过文献挖掘1973-2023年慢性心力衰竭中医证候，总结其中医证候特征演变规律。方法检索1973年1月1日-2023年10月1日中国知网等3个数据库中有关慢性心力衰竭中医证候临床研究的相关文献，使用SPSS 21.0和Rstudio软件对最终纳入文献的中医证候类型和证候要素分布特征进行描述性统计分析、关联规则分析和聚类分析。结果最终纳入218篇文献，包括41604例患者，覆盖全国29个省、市、自治区，其中179篇文献明确报道中医复合证型，共包含31123例患者，常见中医证候类型主要包括气虚血瘀证等；累计提取证候要素71996例次，常见病性证候要素主要包括气虚、血瘀、痰浊等；病位证候要素主要包括心、肝、脾等。不同时期中医证候特征呈现一定演变规律，1973-2023年间，气虚血瘀证、痰瘀互阻证占比呈上升趋势；单纯虚证总体占比1994-2003年较1973-1993年升高，但在2004-2023年均降低，且低于1973-1993年；阳虚水泛证占比呈下降趋势；不同地区证候类型整体由单纯虚证转变为虚实夹杂证，如频数占比最高的华北地区由心肺气虚证转变为气虚血瘀证等；关联规则分析及聚类分析结果显示，各时间段证候要素均存在气虚、血瘀、痰浊、水饮组合，但各个时期兼夹不同，存在一定差异。近年来，慢性心力衰竭病位证候要素心、肝、脾占比总体呈上升趋势，本虚病性证候要素占比总体呈下降趋势，标实病性证候要素占比总体呈上升趋势。结论慢性心力衰竭证候特征总属本虚标实之证，“虚气留滞”为其核心病机，病位主要为心、肝、脾，以气虚、痰浊、血瘀、水饮为主要构成的证候要素逐渐占据主导地位，未来慢性心力衰竭中医证候类型可能呈现“虚痰瘀水”兼夹趋势。

关键词：慢性心力衰竭中医证候证候要素虚气留滞演变规律

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Adsorption of uranium(Ⅵ) onto amidoxime-functionalized ultrahigh molecular weight polyethylene fibers from aqueous solution

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Nuclear Science and Techniques 2017年第7期28卷 97-104页

作者： Chun-yu Xie Shi-Pei Jing yu wang Xiao Lin Hong-Liang Bao Cheng-Zhi Guan Chan Jin Jian-Qiang wang Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China University of Chinese Academy of Sciences Beijing 100049China

Amidoxime-functionalized ultra-high molecular weight polyethylene fibers(UHMWPEF-AO) were used to absorb uranium U(VI) from aqueous *** this paper,we study effects of pH,initial U(VI) concentration,contact time,and temperature on U(VI) adsorption by *** adsorption process agrees well with pseudo-second-order and Langmuir *** exhibits excellent adsorptive performance for U(VI)with a maximum adsorption capacity of 176.12 mg/g at pH4 and 298 *** structures of UHMWPEF-AO and U(VI)-loaded UHMWPEF-AO are characterized by FT-IR and nano-CT.U(VI)-loaded UHMWPEF-AO is sintered after adsorption process to recycle absorbed U(VI).Powders collected after sintering process are examined by scanning electron microscopy and X-ray *** results indicate that UHMWPEF-AO is a promising candidate to remove U(VI) from uranium aqueous solutions.

关键词：超高分子量聚乙烯纤维吸附性能偕胺肟基铀(Ⅵ) 水溶液扫描电子显微镜烧结过程 FT-IR

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Single Production of Charged Gauge Boson W_H^-from Left-Right Twin Higgs Model in Association with Top Quark at LHC

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Communications in Theoretical Physics 2008年第4期49卷 977-980页

作者： LIU Yao-Bei wang Xue-Lei HAN Hong-mei CAO Yong-Hua Henan Institute of Science and Technology Xinxiang 453003 China College of Physics and Information Engineering Henan Normal University Xinxiang 453007 China

The twin Higgs mechanism has recently been proposed to solve the little hierarchy problem. In the context of the left-right twin Higgs （LRTH） model, we discuss single production of the new charged gauge boson WH^- , which is predicted by the left-right twin Higgs model, in association with top quark at the CERN Large Hadron Collider （LHC）. It is found that, for a typical nonzero value of mass mixing parameter M = 150 GeV in the LRTH model, the production cross section is in the range of 3 ×10^-2 - 6.07×10^3 fb at the LHC. As long as the WH^- is not too heavy, the possible signatures of the heavy charged gauge boson might be detected at the LHC experiments.

关键词： left-right twin Higgs model cross section new charged gauge boson

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In-situ self-templating synthesis of 3D hierarchical porous carbonsfrom oxygen-bridged porous organic polymers for highperformance supercapacitors

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Nano Research 2022年第9期15卷 7759-7768页

作者： Qi Xiong Bei Liu Yijiang Liu Pu wang Hua Cheng Huaming Li Zhouguang Lu mei Yang College of Chemistry Xiangtan UniversityXiangtan 411105China Key Laboratory of Polymeric Materials&Application Technology of Hunan Province Key Laboratory of Advanced Functional Polymeric Materialsof College of Hunan Provinceand Key Lab of Environment-Friendly Chemistry and Application in Ministry of EducationXiangtan UniversityXiangtan 411105China Department of Materials Science&Engineering Shenzhen Key Laboratory of Interfacial Science and Engineering of MaterialsSouthern Universityof Science and TechnologyShenzhen 518055China

It is a big challenge to well control the porous structure of carbon materials for supercapacitor ***,a simple in-situ self-templating strategy is developed to prepare three-dimensional(3D)hierarchical porous carbons with good combination of micro and meso-porous architecture derived from a new oxygen-bridged porous organic polymer(OPOP).The OPOP is produced by the condensation polymerization of cyanuric chloride and hydroquinone in NaOH ethanol solution and NaCl is in-situ formed as by-product that will serve as template to construct an interconnected 3D hierarchical porous architecture upon *** large interface pore architecture,and rich doping of N and O heteroatoms effectively promote the electrolyte accessibility and electronic conductivity,and provide abundant active sites for energy ***,the supercapacitors based on the optimized OPOP-800 sample display an energy density of 8.44 and 27.28 Wh·kg^(−1)in 6.0 M KOH and 1.0 M Na2SO4 electrolytes,*** capacitance retention is more than 94%after 10,000 ***,density functional theory(DFT)calculations have been employed to unveil the charge storage mechanism in the *** results presented in this job are inspiring in finely tuning the porous structure to optimize the supercapacitive performance of carbon materials.

关键词： in-situ self-templating porous organic polymer three-dimensional(3D)hierarchical porous carbons supercapacitors

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Diffusion in Fast-Ion Conductor Calcia-Stabilized Zirconia: a Molecular Dynamics Study

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Chinese Physics Letters 1998年第10期15卷 727-729页

作者： SUN Jing-ze wang yu-ming Department of Materials Science Jilin UniversityChangchun 130023

Molecular dynamics simulation technique is used to investigate the ionic conduction property of the fast-ion conductor calcia-stabilized zirconia(CSZ).The theoretical results coincide with experimental measurements *** is suggested that the dopant cation distribution is a key factor controlling the ionic conduction property of the CSZ electrolytes,which can be used as a rule in the microstructure designing and controlling in material processing.

关键词： Molecular conduction controlling

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Prefrontal Nectin3 Reduction Mediates Adolescent Stress-Induced Deficits of Social Memory,Spatial Working Memory,and Dendritic Structure in Mice

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Neuroscience Bulletin 2020年第8期36卷 860-874页

作者： Hong-Li wang Ji-Tao Li Han wang Ya-Xin Sun Rui Liu Xiao-Dong wang yun-Ai Su Tian-mei Si National Clinical Research Center for Mental Disorders(Peking University Sixth Hospital/Institute of Mental Health)and the Key Laboratory of Mental Health Ministry of Health(Peking University Sixth Hospital)Beijing 100191China The National Clinical Research Center for Mental Disorders&Beijing Key Laboratory of Mental Disorders Beijing Anding HospitalCapital Medical University&the Advanced Innovation Center for Human Brain ProtectionCapital Medical UniversityBeijing 100088China Department of Neurobiology Key Laboratory of Medical Neurobiology of The Ministry of Health of ChinaZhejiang Province Key Laboratory of NeurobiologyZhejiang University School of MedicineHangzhou 310003China

Chronic stress may disrupt the normal neurodevelopmental trajectory of the adolescent brain(especially the prefrontal cortex) and contribute to the pathophysiology of stress-related mental illnesses,but the underlying molecular mechanisms remain ***,we investigated how synaptic cell adhesion molecules(e.g.,nectin3)are involved in the effects of adolescent chronic stress on mouse medial prefrontal cortex(mPFC).Male C57BL/6N mice were subjected to chronic social instability stress from postnatal days 29 to *** week later,the mice exposed to chronic stress exhibited impaired social recognition and spatial working memory,simplified dendritic structure,and reduced spine density in the *** localization of nectin3 was also altered,and was significantly correlated with behavioral ***,knocking down mPFC nectin3 expression by adeno-associated virus in adolescent mice reproduced the stress-induced changes in behavior and mPFC *** results support the hypothesis that nectin3 is a potential mediator of the effects of adolescent chronic stress on prefrontal structural and functional abnormalities.

关键词： Adolescence Chronic stress Cell adhesion molecule Prefrontal cortex Social memory

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Systematic insertion mutagenesis of GntR family tran-scriptional regulator genes in Sinorhizobium meliloti

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Chinese Science Bulletin 2008年第2期53卷 215-226页

作者： yu Aiyuan CHEN AiMin wang Yi LUO Li ZHU JiaBi yu GuanQiao wang YanZhang National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Biological Sciences Department Lehman College The City University of New York New York 104681 USA

GntR-type transcriptional regulators regulate the most diverse biological processes in bacteria. Al- though GntR-type transcriptional regulators consist of the second largest family of transcriptional regulators in Sinorhizobium meliloti, little is known about their functions. In this study, we investigated 54 putative genes encoding GntR family of transcriptional regulators in S. meliloti Rm1021. Secondary structure analysis of the C-terminal domain of these putative transcriptional regulators indicated that thirty-seven were members of the FadR subfamily, ten of the HutC subfamily and five of the MocR subfamily. The remaining two did not fall into any specific subfamily category, and may form two new subfamilies. The 54 gntR genes were mutagenized by plasmid insertion mutagenesis to investigate their roles. We found that, of the 54 mutants, only the gtrA1 and gtrB1 mutants had slower growth rates and cell maximal yields on both rich medium and minimal medium, and lower cell motility on swarming plate than wild type Rm1021. All mutants, with the exception of gtrA1 and gtrB1, can establish effective symbioses with alfalfa. Plants inoculated with gtrA1 and gtrB1 mutants grew shorter than those in- oculated with wild type, and formed relatively smaller, round and light pink nodules, which were mainly located on lateral roots. And there was an abnormal increase in the number of nodules induced by both mutants. These results suggested that the gtrA1 and gtrB1 mutants were symbiotically deficient. Our work presents a global overview of GntR-like transcriptional regulators involved in symbiosis in S. meliloti, and provides new insight into the functions of GntR-like transcriptional regulators.

关键词：细菌诱变质粒插入调节剂

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