检索结果-南通市图书馆

Activation of metabotropic glutamate receptor 1 regulates hippocampal CA1 region excitability in rats with status epilepticus by suppressing the HCN1 channel

引用

Neural Regeneration Research 2023年第3期18卷 594-602页

作者： Xiao-Dan Luo Tao Xiang Si-Jun Li mei-Gang Ma mei-ling chen Yuan Wu Department of Neurology First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi Zhuang Autonomous RegionChina Department of Neurology First Affiliated Hospital of University of South ChinaHengyangHunan ProvinceChina Department of Neurology Affiliated Hospital of Guilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina

Dysregulation of hyperpolarization-activated cyclic nucleotide-gated cation(HCN)channels alters neuronal ***,the role of HCN channels in status epilepticus is not fully *** this study,we established rat models of pentylenetetrazole-induced status *** performed western blot assays and immunofluorescence *** results showed that HCN1 channel protein expression,particularly HCN1 surface protein,was significantly decreased in the hippocampal CA1 region,whereas the expression of HCN2 channel protein was ***,metabolic glutamate receptor 1(mGluR1)protein expression was increased after status *** mGluR1 agonist(RS)-3,5-dihydroxyphenylglycine injected intracerebroventricularly increased the sensitivity and severity of pentylenetetrazole-induced status epilepticus,whereas application of the mGluR1 antagonist(+)-2-methyl-4-carboxyphenylglycine(LY367385)alleviated the severity of pentylenetetrazole-induced status *** results from double immunofluorescence labeling revealed that mGluR1 and HCN1 were co-localized in the CA1 ***,a protein kinase A inhibitor(H89)administered intraperitoneally successfully reversed HCN1 channel inhibition,thereby suppressing the severity and prolonging the latency of pentylenetetrazole-induced status ***,H89 reduced the level of mGluR1,downregulated cyclic adenosine monophosphate(cAMP)/protein kinase A expression,significantly increased tetratricopeptide repeat-containing Rab8b-interacting protein(TRIP8b)(1a-4)expression,and restored TRIP8b(1b-2)***8b(1a-4)and TRIP8b(1b-2)are subunits of Rab8b interacting protein that regulate HCN1 surface protein.

关键词： (RS)-3,5-dihydroxyphenylglycine CA1 region excitability H89 HCN1 channel LY367385 mGluR1 pentylenetetrazole status epilepticus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Artesunate inhibits proliferation and migration of RPE cells and TGF-β2 mediated epithelial mesenchymal transition by suppressing PI3K/AKT pathway

引用

International Journal of Ophthalmology(English edition) 2022年第2期15卷 197-204页

作者： Zi-Yi Wang Yu Zhang ling-Dan Wu Jie chen mei-ling chen Ci-Min chen Qi-Hua Xu The Affiliated Eye Hospital of Nanchang University Jiangxi Clinical Research Center for Ophthalmic DiseaseNanchang 330000Jiangxi ProvinceChina

AIM:To study the braking effectiveness of artesunate on transforming growth factor(TGF)-β2 mediated epithelial-mesenchymal transition(EMT)in retinal pigment epithelium(RPE)in ***:The fostered ARPE-19 cells were processed with artesunate alone or combined with the TGF-β*** CCK-8 examination was utilized to test the cell *** migration was detected by scratch as well as the Transwell *** EMT characters and activation of PI3K/Akt signal channel were estimated by Western blotting and *** Western blotting was utilized in order to confirm the vitreous of controls as well as patients with proliferative vitreoretinopathy(PVR)were collected and the levels of PI3K,phospho-PI3K,***:Disposal of ARPE-19 cells with artesunate(50-150μmol/L)obviously suppressed their propagation and immigration,which dependent on the concentration and *** suppressed the EMT which was induced by TGF-β2 in ARPE-19 cells through sustaining the expression of vimentin andα-SMA through the suppression of PI3K,phospho-PI3K,phospho-Akt and *** of PI3K,phospho-PI3K,AKT and phospho-Akt was increased in the vitreous in PVR(P<0.05).CONCLUSION:Such findings indicate that PI3K/Akt signal channel is highly activated in vitreous of *** is an operative depressor of the propagation,immigration and TGF-β2-mediated EMT of ARPE-19 cells by reduced the expression of PI3K/Akt channel.

关键词： proliferative vitreoretinopathy artesunate epithelial-mesenchymal transition migration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第7期12卷 545-551页

作者： mei-ling chen Qin GUO Rui-zhi WANG Juan XU chen-wei ZHOU Hui RUAN Guo-qing HE Department of Food Science and Nutrition Zhejiang University Hangzhou 310029 China School of Food and Biological Engineering Jiangsu University Zhenjiang 212013 China

Surface display is effectively utilized to construct a whole-cell *** optimization has been proven to be effective in maximizing production of heterologous proteins in ***,the cDNA sequence of Rhizopus oryzae lipase (ROL) was optimized and synthesized according to the codon bias of Saccharomyces cerevisiae,and based on the Saccharomyces cerevisiae cell surface display system with α-agglutinin as an anchor,recombinant yeast displaying fully codon-optimized ROL with high activity was successfully *** with the wild-type ROL-displaying yeast,the activity of the codon-optimized ROL yeast whole-cell biocatalyst (25 U/g dried cells) was 12.8-fold higher in a hydrolysis reaction using p-nitrophenyl palmitate (pNPP) as the *** our knowledge,this was the first attempt to combine the techniques of yeast surface display and codon optimization for whole-cell biocatalyst ***,the yeast whole-cell ROL biocatalyst was constructed with high *** optimum pH and temperature for the yeast whole-cell ROL biocatalyst were pH 7.0 and 40 °***,this whole-cell biocatalyst was applied to the hydrolysis of tributyrin and the resulted conversion of butyric acid reached 96.91% after 144 h.

关键词： Rhizopus oryzae lipase (ROL) Yeast surface display Codon optimization Whole-cell biocatalyst

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Global research trends in cardiac arrest research:a visual analysis of the literature based on CiteSpace

引用

World Journal of Emergency Medicine 2022年第4期13卷 290-296页

作者： Shi-jiao Yan mei chen Jing Wen Wen-ning Fu Xing-yue Song Huan-jun chen Ri-xing Wang mei-ling chen Xiao-tong Han Chuan-zhu Lyu School of Public Health Hainan Medical UniversityHaikou 570100China Research Unit of Island Emergency Medicine Chinese Academy of Medical Sciences(No.2019RU013)Hainan Medical UniversityHaikou 570100China Guizhou Center for Disease Control and Prevention Guiyang 550004China School of Nursing Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430000China Key Laboratory of Emergency and Trauma Ministry of EducationCollege of Emergency and TraumaHainan Medical UniversityHaikou 571199China Department of Emergency Hainan Clinical Research Center for Acute and Critical Diseasesthe Second Affiliated Hospital of Hainan Medical UniversityHaikou 570100China Emergency and Trauma College Hainan Medical UniversityHaikou 570100China Department of Emergency Medicine Hunan Provincial Institute of Emergency MedicineHunan Provincial Key Laboratory of Emergency and Critical Care MetabolomicsHunan Provincial People's Hospital/the First Affiliated HospitalHunan Normal UniversityChangsha 410000China Emergency Medicine Center Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China

BACKGROUND:The high morbidity,high mortality and low survival rate of cardiac arrest(CA)cause a heavy global *** aimed to analyze the changes in scientific output related to CA over the past two ***:We analyzed the scientific output related to CA from 2000 to 2020 via the Web of *** data were analyzed using CiteSpace ***:In total,28,312 articles relating to CA were identified in the Web of *** volume of scientific research output in the field of global CA research was mainly distributed in the Americas,Europe and Asia,covering a wide *** the 28,312 articles,the research content of the highly cited literature mainly focused on CA,mild hypothermia treatment,and prognosis of CA ***:Various scientific methods were applied to reveal scientific productivity,collaboration,and research hotspots in the CA research *** resuscitation(CPR),extracorporeal membrane oxygenation(ECMO),survival and target temperature management are research *** research on CA will continue to focus on its treatment and prognosis to improve the survival rate of CA patients.

关键词： Cardiac arrest Bibliometrics CiteSpace Research hotspots Cardiopulmonary resuscitation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Strengthening of HT250 by modified ultra-fine ceramic powders

引用

China Foundry 2015年第6期12卷 418-424页

作者： Bo Yuan mei-ling chen Jun Yang Gui-lin Liu Zhi-ming Yan Collage of Materials Science and Engineering Dalian Jiaotong University

In this study, three kinds of modified ultra-fine ceramic powders marked A, B and C, which were prepared by each of three different modifiers mixing with a commercial SiC, were added to HT250 cast iron, respectively, and the effects of the modified ultra-fine ceramic powders on microstructure, mechanical properties and wear resistance were studied. Metallographic examination, tensile test, scanning electron microscopy, and three-dimensional surface topography were applied to analyze and compare the samples containing modified powder with the original samples. The results showed that the most obvious modification effect among the powders was seen in the sample containing powder A, with the graphite and eutectic cells being refined, the tensile strength being increased by 36.9%, and the wear resistance being improved by 45.5% and 47.2% under loads of 150 N and 300 N, respectively. The improvements of mechanical properties and wear resistance in the HT250 cast iron with the modified ultra-fine ceramic powders were attributed to the synergistic effect of the grain refinement with the powder acting as a hard particle phase and the lubrication by the graphite.

关键词： modified ultra-fine ceramic powders HT250 microstructure mechanical properties wear resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A case of mimicking angioedema： chin silicone granulomatous reaction spreading all over the face after receiving liquid silicone injection forty years previously

引用

Chinese Medical Journal 2011年第11期124卷 1747-1750页

作者： Yu-cheng chen mei-ling chen Ying-ming Chiu Division of EndOcrinology and Metabolism Department ofInternal Medicine Department of Pathology Division of Rheumatology Department of Internal MedicineChanghua Christian I~Iospital Changhua TaiwanChina

Liquid injectable silicone has been used for soft tissue augmentation for five decades. Many complications following liquid silicone injection have been reported. To diagnose and manage silicone granuloma remains difficult. Silicone granuloma must be diagnosed with the history of liquid silicone injection and the histology of tissue biopsy. We presented a case of granulomatous reaction after the injection of liquid silicone for chin augmentation forty years ago, causing total facial swelling, which mimicking angioedema initially. We administered methylprednisolone to the patient. Initial response to methylprednisolone was favorable.

关键词： silicone granuloma methylprednisolone angioedema liquid silicone

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

T3098C and T53C Mutations of HBV Genotype C Is Associated with HBV Infection Progress

引用

Biomedical and Environmental Sciences 2009年第6期22卷 511-517页

作者： SU-ZHEN JIANG ZHI-YONG GAO TONG LI ,YA-JUAN LI XIANO-mei chen ling WANG FENG-MIN LU HUI ZHUANG Department of Microbiology Peking University Health Science Center Beijing 100191 China

Objective To analyze the association between mutation（s） in preS region of HBV and hepatitis B disease progress in Chinese patients with genotype C chronic HBV infection. Methods Ninety-three patients with chronic genotype C HBV infection, including 24 asymptomatic carriers （ASC）, 26 patients with chronic hepatitis B （CHB）, 22 patients with liver cirrhosis （LC） and 21 HCC patients were investigated. Levels of HBV DNA, HBeAg, alanine aminotransferase （ALT）, asparate transaminase （AST） were measured. HBV preS region was analyzed by PCR direct sequencing. Results The prevalence of preS T3098C and T53C mutations ofgenotype C HBV was significantly higher in LC and HCC patients than ASC and CHB patients. The rate ofT3098C mutation in ASC, CHB, LC, and HCC patients were 0.00% （0/24）, 3.85% （1/26）, 9.09% （2/22）, and 30.77% （8/22）, respectively （P=0.0015）, while the rate of T53C mutation was I2.50% （3/24）, 3.85% （1/26）, 40.91% （9/22）, and 42.31% （11/26）, respectively （P=0.0012）. Conclusion The frequency of genotype C HBV preS T3098C and T53C mutations is associated with hepatitis B infection progression.

关键词： Hepatitis B virus (HBV) Genotype C pre S Mutation Hepatocellular carcinoma (HCC)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Withaferin A inhibits ferroptosis and protects against intracerebral hemorrhage

引用

Neural Regeneration Research 2023年第6期18卷 1308-1315页

作者： Zi-Xian Zhou Qi Cui Ying-mei Zhang Jia-Xin Yang Wen-Jing Xiang Ning Tian Yan-Lin Jiang mei-ling chen Bin Yang Qing-Hua Li Ru-Jia Liao Laboratory of Neuroscience Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina Department of Neurology Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina Guangxi Clinical Research Center for Neurological Diseases Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina Department of Pharmacology Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina

Recent studies have indicated that suppressing oxidative stress and ferroptosis can considerably improve the prognosis of intracerebral hemorrhage(ICH).Withaferin A(WFA),a natural compound,exhibits a positive effect on a number of neurological ***,the effects of WFA on oxidative stress and ferroptosis-mediated signaling pathways to ICH remain *** this study,we investigated the neuroprotective effects and underlying mechanism for WFA in the regulation of ICH-induced oxidative stress and *** established a mouse model of ICH by injection of autologous tail artery blood into the caudate nucleus and an in vitro cell model of hemin-induced *** was injected intracerebroventricularly at 0.1,1 or 5μg/kg once daily for 7 days,starting immediately after ICH *** markedly reduced brain tissue injury and iron deposition and improved neurological function in a dose-dependent manner 7 days after cerebral *** in vitro experiments,cell viability test showed that WFA protected SH-SY5Y neuronal cells against hemin-induced cell ***-linked immunosorbent assays in vitro and in vivo showed that WFA markedly decreased the level of malondialdehyde,an oxidative stress marker,and increased the activities of anti-oxidative stress markers superoxide dismutase and glutathione peroxidase after *** blot assay,quantitative polymerase chain reaction and immunofluorescence results demonstrated that WFA activated the nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)signaling axis,promoted translocation of Nrf2 from the cytoplasm to nucleus,and increased HO-1 *** Nrf2 with siRNA completely reversed HO-1 expression,oxidative stress and protective effects of ***,WFA reduced hemin-induced ***,after treatment with an HO-1 inhibitor,the neuroprotective effects of WFA against hemin-induced ferroptosis were *** test results showed that WFA combined with ferrostatin-1

关键词： behavior brain injuries hemorrhagic stroke ferroptosis heme oxygenase-1 neuroprotection nuclear factor E2-related factor 2 nuclear translocator oxidative stress stroke

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Treating aplasia cutis congenita in a newborn with the combination of ionic silver dressing and moist exposed burn ointment: A case report

引用

World Journal of Clinical Cases 2019年第17期7卷 2611-2616页

作者： Guo-Feng Lei Jun-Ping Zhang Xiao-Bing Wang Xiao-Li You Jin-Ya Gao Xiao-mei Li mei-ling chen Xiu-Qin Ning Jiang-Li Sun Department of Neonatal Intensive Care Unit Sanmenxia Central HospitalSanmenxia 472000Henan ProvinceChina Department of Intensive Care Unit Sanmenxia Central HospitalSanmenxia 472000Henan ProvinceChina Reproductive endocrine center Sanmenxia Central HospitalSanmenxia 472000Henan ProvinceChina

BACKGROUND Aplasia cutis congenita (ACC) in newborns is a condition in which congenital defects or hypoplasia is present in part of the epidermis,dermis and even subcutaneous tissue (including muscle and bones).First reported by Cordon in 1767,ACC is a rare disease with a low incidence of 1/100000 to 3/***,there are 500 cases reported *** can be accompanied by other *** onset mechanism of the disease remains unknown but is thought to be correlated to factors such as genetics,narrow uterus,foetal skin and amniotic membrane adhesion,use of teratogenic drugs in early pregnancy and viral *** SUMMARY In August 2018,we treated a newborn with ACC on the left lower limbs using a combination of ionic silver dressing and moist exposed burn ointment (MEBO) and achieved a satisfactory treatment *** skin defects were observed on the external genitals and on areas from the left foot to 3/4 of the upper left *** tissue and blood vessels were observed in the regions with skin *** following treatments were ***,the wound was rinsed with 0.9% sodium chloride solution followed by disinfection with povidone-iodine *** then MEBO was applied to the wound at a thickness of approximately 1 *** applying ionic silver dressing,the wound was covered with sterile *** wound dressing was replaced every 2-3 *** the 4-mo follow-up,the treatment outcome was *** was minimal scar tissue formation,and limb function was not *** The combination of ionic silver dressing and MEBO to ACC is helpful.

关键词： Aplasia cutis congenita Newborns Ionic silver dressing Moist exposed burn ointment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preparation of Low Ratio Magnesium Ccement by Acid Leaching Treatment of Boron Mud

引用

Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 120-129页

作者： YANG mei ZHANG Mingzhe chen Hao School of Materials Science and Engineering Shenyang Jianzhu UniversityShenyang 110168China School of Physical Science and Technology Shenyang Normal UniversityShenyang 110168China

The effects of liquid-solid ratio and reaction time on the leaching rate of magnesium at room temperature were investigated,as well as the effects of the molar ratio of MgO/MgCl_(2),the amount of water added,and the amount of acid-impregnated slag dosed on the compressive strength and water resistance of *** results showed that the magnesium element in the boron mud could be maximally leached under the conditions of 1:1 concentration of hydrochloric acid at room temperature,liquid-solid ratio of 2.5 mL·g^(-1),and reaction time of 5 h,and the main products were amorphous SiO_(2) as well as a small amount of magnesium olivine which had not been completely *** LR-MOC prepared using the acid-soaked mixture could reach a softening coefficient of 0.85 for 28 d of water immersion when the molar ratio of MgO/MgCl_(2) was 2.2,the amount of water added was 0 g,and the acid-soaked slag dosing was 40 wt%,which also led to an appreciable late-strength,with an increase of 19.4%in compressive strength at 28 d compared to that at 7 *** previous studies,LR-MOC prepared in this way has a final strength phase that is not the more easily hydrolysed 3-phase but the lath-like *** this phenomenon,we analyzed the mechanism and found that,during the acid leaching process,a part of amorphous SiO_(2) dissolved in the acid leaching solution formed a silica sol,in which Mg^(2+)played a bridging role to make the silica sol more *** the addition and hydrolysis of MgO,the silica sol gel coagulation slows down,providing a capping layer to inhibit the hydrolysis of the 5-phase crystals and providing some strength after *** amorphous SiO_(2) in the other part of the acid-impregnated slag generated M-S-H gel with Mg^(2+)and OH-,which synergised with the dense structure composed of interlocking crystals to improve the water resistance of LR-MOC.

关键词： boron mud magnesium chloride-oxygen cement acid immersion water resistance silica sol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：