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长根菇静置培养液体菌种培养基的优化

安徽农业科学 2008年第5期36卷 1926-1928页

作者：胡梅河南科技大学林学院河南洛阳471003

[目的]为更好地开发和利用长根菇这一珍稀食、药用菌资源。[方法]以菌丝体生物量为测定指标,采用静置培养的方法探索长根菇液体菌种的最佳培养基配方。[结果]通过单因素试验和正交试验,确定静置培养长根菇液体菌种的最佳培养基配方为:4... 详细信息

[目的]为更好地开发和利用长根菇这一珍稀食、药用菌资源。[方法]以菌丝体生物量为测定指标,采用静置培养的方法探索长根菇液体菌种的最佳培养基配方。[结果]通过单因素试验和正交试验,确定静置培养长根菇液体菌种的最佳培养基配方为:4.0%玉米淀粉、0.2%黄豆饼粉、2.0%葡萄糖、0.2%蛋白胨、0.2%KH2PO4、0.1%MgSO4、0.1%CaSO4和60.0 mg/L VB1。在此培养基中,在25～26℃静置培养8 d后,长根菇菌丝体生物量可达8.75 g/L。[结论]由于优化的培养基中主要原料价格较低,因此将此培养基配方用于生产将会降低生产成本。

关键词：长根菇静置培养液体菌种培养基生物量

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基于工效学的机车前窗刮雨器尺寸参数的设计

铁道学报 2005年第6期27卷 34-38页

作者：胡清梅方卫宁李伏京北京交通大学机械与电子控制工程学院北京100044

从工效学角度,对机车前窗刮雨器尺寸参数的设计方法进行了深入的探讨。结合SAE(美国汽车工程师协会)标准和我国铁路行业标准,确定了适合于我国机车特点的前窗刮扫视野范围。然后,以眼椭圆理论为依据,提出了一种基于工效学的前窗刮雨器... 详细信息

从工效学角度,对机车前窗刮雨器尺寸参数的设计方法进行了深入的探讨。结合SAE(美国汽车工程师协会)标准和我国铁路行业标准,确定了适合于我国机车特点的前窗刮扫视野范围。然后,以眼椭圆理论为依据,提出了一种基于工效学的前窗刮雨器尺寸参数及安装位置的设计方法,该方法能有效满足机车司机前方视野瞭望的要求。

关键词：工效学机车刮雨器尺寸参数

Multi-dimensional Central Limit Theorems and Laws of Large Numbers under Sublinear Expectations

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Acta Mathematica Sinica,English Series 2015年第2期31卷 305-318页

作者： Ze Chun hu Ling ZHOU Department of Mathematics Nanjing University

In this paper, we present some multi-dimensional central limit theorems and laws of large numbers under sublinear expectations, which extend some previous results.

关键词： Central limit theorem law of large numbers sublinear expectation

Reentry Trajectory Optimization for Hypersonic Vehicles Using Fuzzy Satisfactory Goal Programming Method

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International Journal of Automation and computing 2015年第2期12卷 171-181页

作者： Chao-Fang hu Yue Xin School of Electrical Engineering and Automation Tianjin University

Constrained reentry trajectory optimization for hypersonic vehicles is a challenging job. In particular, this problem becomes more difficult when several objectives with preemptive priorities are expected for different purposes. In this paper, a fuzzy satisfactory goal programming method is proposed to solve the multi-objective reentry trajectory optimization problem. Firstly, direct collocation approach is used to discretize the reentry trajectory optimal-control problem with nonlinear constraints into nonlinear multiobjective programming problem with preemptive priorities, where attack angles and bank angles at nodes and collocation nodes are selected as control variables. Secondly, the preemptive priorities are transformed into the relaxed order of satisfactory degrees according to the principle that the objective with higher priority has higher satisfactory degree. Then the fuzzy satisfactory goal programming model is proposed. The balance between optimization and priorities is realized by regulating parameter λ, such that the satisfactory reentry trajectory can be acquired. The simulation demonstrates that the proposed method is effective for the multi-objective reentry trajectory optimization of hypersonic vehicles.

关键词： Reentry trajectory optimization direct collocation method multi-objective optimization fuzzy satisfactory method goal programmin

One-Pot Synthesis of Co-Based Coordination Polymer Nanowire for Li-Ion Batteries with Great Capacity and Stable Cycling Stability

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Nano-Micro Letters 2018年第2期10卷 3-11页

作者： Peng Wang Xiaobing Lou Chao Li Xiaoshi hu Qi Yang Bingwen hu State Key Laboratory of Precision Spectroscopy Shanghai Key Laboratory of Magnetic Resonance School of Physics and Materials Science East China Normal University

Nanowire coordination polymer cobalt–terephthalonitrile(Co-BDCN) was successfully synthesized using a simple solvothermal method and applied as anode material for lithium-ion batteries(LIBs). A reversible capacity of 1132 mAh g^(-1) was retained after 100 cycles at a rate of 100 mAg^(-1), which should be one of the best LIBs performances among metal organic frameworks and coordination polymers-based anode materials at such a rate. On the basis of the comprehensive structural and morphology characterizations including fourier transform infrared spectroscopy,~1 H NMR,^(13)C NMR, and scanning electron microscopy, we demonstrated that the great electrochemical performance of the as-synthesized Co-BDCN coordination polymer can be attributed to the synergistic effect of metal centers and organic ligands, as well as the stability of the nanowire morphology during cycling.

关键词： Nanowire Coordination polymer Lithiumion battery Anode Ultra-high capacity

Improving Baculovirus Transduction of Mammalian Cells by Incorporation of Thogotovirus Glycoproteins

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Virologica Sinica 2019年第4期34卷 454-466页

作者： Liangbo hu Yimeng Li Fei Deng Zhihong hu hualin Wang Manli Wang State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of the Chinese Academy of Sciences Beijing 100049China

Baculovirus can transduce a wide range of mammalian cells and is considered a promising gene therapy vector. However,the low transduction efficiency of baculovirus into many mammalian cells limits its practical application. Co-expressing heterologous viral glycoproteins(GPs), such as vesicular stomatitis virus G protein(VSV G), with baculovirus native envelope protein GP64 is one of the feasible strategies for improving virus transduction. Tick-borne thogotoviruses infect mammals and their GPs share sequence/structure homology and common evolutionary origins with baculovirus ***, we tested whether thogotovirus GPs could facilitate the entry of the prototype baculovirus Autographa californica multiple multiple nucleopolyhedrovirus(AcMNPV) into mammalian cells. The gp genes of two thogotoviruses, Thogoto virus and Dhori virus, were inserted into the AcMNPV genome. Both GPs were properly expressed and incorporated into the envelope of the recombinant AcMNPVs. The transduction rates of recombinant AcMNPVs expressing the two thogotovirus GPs increased for approximately 4–12 fold compared to the wild type AcMNPV in six of the 12 tested mammalian cell lines. It seemed that thogotovirus GPs provide the recombinant AcMNPVs with different cell tropisms and showed better performance in several mammalian cells compared to VSV G incorporated AcMNPV. Further studies showed that the improved transduction was a result of augmented virus-endosome fusion and endosome escaping, rather than increased cell binding or internalization. We found the AcMNPV envelope protein GP64-mediated fusion was enhanced by the thogotovirus GPs at relatively higher p H conditions. Therefore, the thogotovirus GPs represent novel candidates to improve baculovirus-based gene delivery vectors.

关键词： Autographa californica multiple nucleopolyhedrovirus(AcMNPV) Baculovirus Thogotovirus Glycoprotein Transduction Mammalian cells

Treatment of multiple sclerosis by transplantation of neural stem cells derived from induced pluripotent stem cells

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Science China(Life Sciences) 2016年第9期59卷 950-957页

作者： Chao Zhang Jiani Cao Xiaoyan Li Haoyu Xu weixu Wang Libin Wang Xiaoyang Zhao wei Li Jianwei Jiao Baoyang hu Qi Zhou Tongbiao Zhao State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Multiple sclerosis(MS) is an autoimmune disease of the central nervous system(CNS), with focal T lymphocytic infiltration and damage of myelin and axons. The underlying mechanism of pathogenesis remains unclear and there are currently no effective treatments. The development of neural stem cell(NSC) transplantation provides a promising strategy to treat neurodegenerative disease. However, the limited availability of NSCs prevents their application in neural disease therapy. In this study, we generated NSCs from induced pluripotent stem cells(iPSCs) and transplanted these cells into mice with experimental autoimmune encephalomyelitis(EAE), a model of MS. The results showed that transplantation of iPSC-derived NSCs dramatically reduced T cell infiltration and ameliorated white matter damage in the treated EAE mice. Correspondingly, the disease symptom score was greatly decreased, and motor ability was dramatically rescued in the iPSC-NSC-treated EAE mice, indicating the effectiveness of using iPSC-NSCs to treat MS. Our study provides pre-clinical evidence to support the feasibility of treating MS by transplantation of iPSC-derived NSCs.

关键词： induced pluripotent stem cell multiple sclerosis neural stem cell regenerative medicine transplantation

RAPD analysis on the four kinds of color-flowered Salvia splendens Ker-Gawl

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Journal of Northeast Agricultural University(English Edition) 2007年第2期14卷 97-102页

作者： hu Guofu YUAN Qiang LI Fenglan wei Qi hu Baozhong College of Life Sciences Northeast Agricultural University Harbin 1500300 China

In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture （25 μL total volume） that contained 2.0 μL 10×buffer, 0.45 mmol·L^-1 dNTPs, 2.0 mmol· L^-1 Mg^2＋, 2 U Taq DNA polymerase, 0.30 umol·L^-2 primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style.

关键词： Salvia splendens Ker-Gawl color RAPD analysis cluster group analysis

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电子公共服务战略规划方法研究及实证

管理科学学报 2008年第3期11卷 35-48页

作者：胡广伟仲伟俊梅姝娥东南大学经济管理学院南京210096 南京大学信息管理系南京210093

从业务层与职能层的角度研究了地方政府电子公共服务战略规划问题.首先,提出一个包括4个阶段15步的战略规划方法,详细讨论了电子公共服务的战略规划过程,并提出研究的理论假设.然后,以问卷调查及年鉴数据为基础,对与战略环境相关的理论... 详细信息

从业务层与职能层的角度研究了地方政府电子公共服务战略规划问题.首先,提出一个包括4个阶段15步的战略规划方法,详细讨论了电子公共服务的战略规划过程,并提出研究的理论假设.然后,以问卷调查及年鉴数据为基础,对与战略环境相关的理论假设进行了实证研究.实证研究验证了战略规划方法中所讨论环境影响因素的合理性,支持了文中所提出规划方法的有效性.该方法提出后的应用情况表明,它是科学、合理与可操作的.

关键词：电子政务公共服务战略规划行政环境议题分析