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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： mei-qing xing,hong-wei xue~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200032 people’s republic of china

A proteome study based on multi-dimensional non-gel shotgun approach was performed in order to analyze the auxin-induced protein degradation via ubiquitin-proteasome pathway of Arabidopsis *** emphasis was to monitor the overall changes in the protein complexes after auxin *** and 1uM IAA were used to treat 7-day-old seedlings of col-0 and tir1-1 at three time points--6,12,24 hours,more than

关键词： shotgun proteomics auxin protein degradation label-free quantification Arabidopsis

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Identification of ABA-responsive genes in rice shoots via cDNA macroar- ray

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Cell Research 2003年第1期13卷 59-68页

作者： FANG LIN, sHOU LING XU, wei MIN NI, ZHAO qing CHU, ZHI hong XU, hong wei xuenational laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological science (sIBs), chinese academy of sciences and Partner Group of Max-Planck-institute of molecular plant physiology (MPI-MP) on "plant molecular physiology and signal Transduc-tion", 300 Fenglin Road, 200032 shanghai, china national laboratory of plant molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-institute of molecular plant physiology (MPI-MP) on plant molecular physiology and signal Transduction Shanghai China

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In add

关键词： Oryza saliva abscisic acid (ABA) cDNA macroarray responsive genes signal transduc- tion

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Heterotrimeric G-protein controls chloroplast development via FtsH protease

Heterotrimeric G-protein controls chloroplast development vi...

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2006年拟南芥研究学术研讨会

作者： qing wei, Wenjuan Wu, Guangzhen Hu, Fenhong Hu, Jirong Huang national key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china

＜正＞Chloroplasts not only endow higher plants with a distinct ability of photosynthesis but also hold many metabolic pathways that are essential for biosynthesis of amino acids, lipids, pigments and hormones, and thus play an important role in crop yield and stress resistance. For sake of a long period of symbiotic evolution, chloroplast development including number, volume and composition is dynamically coordinated with cell sizes and types, and ever changing environmental conditions. Although a marked progress has

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Activation of GPA1, the Heterotrimeric G-protein-subunit, suppresses the ftsh-Mediated Inhibition of Chloroplast Development in Arabidopsis

Activation of GPA1, the Heterotrimeric G-protein-Subunit, Su...

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纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Lingang Zhang, qing wei, Wenjuan Wu, Yuxiang Cheng, Guangzhen Hu, Fenhong Hu, Ying Zhu, Jirong Huang national key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china

＜正＞The involvement of the heterotrimeric G-protein signaling pathway in chloroplast development has been demonstrated only by pharmaceutical evidence. In contrast, G-protein knockout mutants have no phenotypic defe... 详细信息

关键词： GPA1 heterotrimeric G-protein-subunit ftsh-Mediated inhibition Chloroplast development Arabidopsis

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OXP1,a Vulnerable Protein to Oxidative Modification,Plays a Role in starch Accumulation and Development in Arabidopsis

OXP1,a Vulnerable Protein to Oxidative Modification,Plays a ...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Xun-Liang Liu~1,Hai-Dong Yu~1,Yuan Guan,Fang-qing Guo~* The national key laboratory of plant molecular genetics,institute of plant physiology & ecology, shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Road, shanghai200032,china

Carbonylation,an oxidative modification of proteins,is most commonly occurring during abiotic *** is thought to be an irreversible protein damage process and can be used as an overall protein oxidation *** modification usually leads to the loss of function of target proteins,which undergo proteolytic degradation or form

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studies on the Rice seed Development Regulation

Studies on the Rice Seed Development Regulation

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2009整合植物生物学研讨会

作者： Bi-qing Wen,Liang-Jiao xue,Lin-Lin Yin,Jing-Jing Zhang and hong-wei xue~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences(sIBs),chinese academy of sciences, shanghai 20032,china.

Rice provides the staple food for more than half of the world’s population and people are increasingly demanding high-yield and high-quality *** into the seed development of rice are important from both a basic devel...

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Ubiquitination of CONsTANs by COP1 in Cryptochrome Regulation of Photoperiodic Flowering in Arabidopsis

Ubiquitination of CONSTANS by COP1 in Cryptochrome Regulatio...

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中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： Li-Jun Liu~1,Yan-Chun Zhang~2,qing-Hua Li~1,Yi sang~1,Jian Mao~1,hong-Li Lian~2,Long Wang~1,and hong-Quan Yang~(1,2*) 1 national key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200032,china 2 school of Agriculture and Biology,shanghai Jiaotong University,shanghai 200240,china

In many plants,the transition from vegetative to reproductive development is controlled by the daily duration of light and/or darkness(i.e.,the photoperiod).In Arabidopsis thaliana, long days(LDs) accelerate flowering... 详细信息

关键词： Arabidopsis CONsTANs cryptochrome flowering FLOWERING LOCUs T ubiquitination

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Rice Homeobox Transcription Factor HOX1a Positively Regulates Gibberellin Responses by Directly suppressing EL1

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Journal of Integrative plant Biology 2011年第11期53卷 869-878页

作者： Bi-qing Wen mei-qing xing Hua Zhang Cheng Dai hong-wei xue national key laboratory of plant-molecular genetics Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China

Homeobox transcription factors are involved in various aspects of plant development, including maintenance of the biosynthesis and signaling pathways of different hormones. However, few direct targets of homeobox proteins have been identified. We here show that overexpression of rice homeobox gene HOXla resulted in enhanced gibberellin （GA） response, indicating a positive effect of HOXla in GA signaling. HOXla is induced by GA and encodes a homeobox transcription factor with transcription repression activity. In addition, HOXla suppresses the transcription of early flowering1 （EL1）, a negative regulator of GA signaling, and further electrophoretic mobility shift assay and chromatin immunoprecipitation analysis revealed that HOXla directly bound to the promoter region of EL1 to suppress its expression and stimulate GA signaling. These results demonstrate that HOXla functions as a positive regulator of GA signaling by suppressing EL1, providing informative hints on the study of GA signaling.

关键词： EL1 giberellin giberellin signaling HOXla homeobox.

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studies on the Rice LEAF INCLINATION1 （LC1）, an IAA-amido synthetase, Reveal the Effects of Auxin in Leaf Inclination Control

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molecular plant 2013年第1期6卷 174-187页

作者： shu-qing Zhao Jing-Jing Xiang hong-wei xue national key laboratory of plant molecular genetics institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences ChineseAcademy of Sciences Shanghai 200032 China

The angle of rice leaf inclination is an important agronomic trait and closely related to the yields and archi- tecture of crops. Although few mutants with altered leaf angles have been reported, the molecular mechanism remains to be elucidated, especially whether hormones are involved in this process. Through genetic screening, a rice gain-of- function mutant leaf inclination1, Icl-D, was identified from the shanghai T-DNA Insertion Population （sHIP）. Phenotypic analysis confirmed the exaggerated leaf angles of Icl-D due to the stimulated cell elongation at the lamina joint. LC1 is transcribed in various tissues and encodes OsGH3-1, an indole-3-acetic acid （IAA） amido synthetase, whose homolog of Arabidopsis functions in maintaining the auxin homeostasis by conjugating excess IAA to various amino acids. Indeed, recombinant LC1 can catalyze the conjugation of IAA to Ala, Asp, and Asn in vitro, which is consistent with the decreased free IAA amount in Icl-D mutant. Icl-D is insensitive to IAA and hypersensitive to exogenous BR, in agreement with the microarray analysis that reveals the altered transcriptions of genes involved in auxin signaling and BR biosynthesis. These results indicate the crucial roles of auxin homeostasis in the leaf inclination control.

关键词： rice leaf inclination auxin GH3.

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A Proteomics study of Auxin-Induced Protein Degradation in Arabidopsis thaliana

A Proteomics Study of Auxin-Induced Protein Degradation in A...

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2010全国植物生物学研讨会

作者： mei-qing xing hong-wei xue national key laboratory of plant molecular genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological Sciences Chinese Academy of Sciences

关键词： shotgun proteomics auxin protein degradation label-free quantification Arabidopsis

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