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mgr-mir-9 implicates Meloidogyne graminicola infection in rice by targeting the effector MgPDI

Journal of Integrative Agriculture 2023年第5期22卷 1445-1454页

作者： TIAN Zhong-ling ZHOU Jia-yan ZHENG Jing-wu HAN shao-jie Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province Interdisciplinary Research Academy(IRA)Zhejiang Shuren UniversityHangzhou 310015P.R.China Laboratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture&BiotechnologyZhejiang UniversityHangzhou 310058P.R.China Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests Zhejiang UniversityHangzhou 310058P.R.China

MicroRNAs (miRNAs),a class of small non-coding RNAs,are crucial endogenous gene regulators in a range of animals,including plant-parasitic *** graminicola is an obligate sedentary endoparasite of rice and causes significant yield losses.A number of studies focused on the roles of *** effectors during the parasitic process;however,how nematode miRNAs regulate its effectors needs *** this research,we analyzed a cluster of *** miRNAs obtained at the second-stage juveniles (J2s) stage that are closely linked to the regulation of *** *** are 49 767 105 total clean reads obtained from three libraries.A total of 233 known miRNAs and 21 novel miRNAs were *** the known miRNAs,mgr-lin-4,mgr-mir-1,mgr-mir-100,mgrmir-86,mgr-mir-279,mgr-mir-87,mgr-mir-71,mgr-mir-9,mgr-mir-50,mgr-mir-72,and mgr-mir-34 are the most abundant11 miRNAs ***,the expression levels of selected miRNAs were validated by real-time quantitative *** hypothesized that these miRNAs might regulate the expression of secreted effectors during the J2s stage to facilitate its *** with this,we found that mgr-mir-9 targets MgPDI,an important *** effector *** addition to that,J2s treated with mgr-mir-9 mimics showed down-regulation of MgPDI expression and reduced reproductive ability,alluding mgr-mir-9 is involved in nematode *** results provide novel insight into the regulatory functions of *** miRNAs during the infection and identify miRNAs and their effector targets as potential key management targets to limit parasite survival during the early stages of infection.

关键词： microRNA function Meloidogyne graminicola deep sequencing Dual-Luciferase Reporter Assay System proteindisulfideisomerase

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Slime coating of kaolinite on chalcopyrite in saline water flotation

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International Journal of Minerals,Metallurgy and Materials 2018年第5期25卷 481-488页

作者： Zhi-li Li Feng Rao shao-xian Song Yan-mei Li Wen-biao Liu CONACYT Instituto de Investigación en Metalurgiay Materiales Universidad Michoacana de San Nicolás de Hidalgo School of Resources and Environmental Engineering Wuhan University of Technology Departamento de Ingeniería en Minas Metalurgia y GeologíaUniversidad de Guanajuato Faculty of Land and Resource Engineering Kunming University of Science and Technology

In saline water flotation, the salinity can cause a distinguishable slime coating of clay minerals on chalcopyrite particles through its effect on their electrical double layers in aqueous solutions. In this work, kaolinite was used as a representative clay mineral for studying slime coating during chalcopyrite flotation. The flotation of chalcopyrite in the presence and absence of kaolinite in tap water, seawater, and gypsum-saturated water and the stability of chalcopyrite and kaolinite particles in slurries are presented. Zeta-potential distributions and scanning electron microscopy images were used to characterize and explain the different slime coating degrees and the different flotation performances. Kaolinite particles induced slime coating on chalcopyrite surfaces and reduced chalcopyrite floatability to the greatest extent when the p H value was in the alkaline range. At 0.24 wt% of kaolinite, the chalcopyrite floatability was depressed by more than 10% at alkaline p H levels in tap water. Salinity in seawater and gypsum-saturated water compressed the electrical double layers and resulted in extensive slime coating.

关键词： chalcopyrite clay minerals saline water flotation slime coating stability

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Deep-detection of formation boundary using transient multicomponent electromagnetic logging measurements

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Petroleum Science 2022年第3期19卷 1085-1098页

作者： Xi-Yong Yuan shao-Gui Deng Zhi-Qiang Li Xiao-mei Han Xu-Fei Hu Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)QingdaoShandong266580China Sinopec Matrix Corporation QingdaoShandong266000China China Research Institute of Radiowave Propagation XinxiangHenan453000China China University of Petroleum—Beijing at Karamay KaramayXinjiang834000China

The maximum distance at which an electromagnetic(EM)logging while drilling(LWD)tool detects an approaching boundary is defined as the depth of detection(DOD).Ultra-deep detection capability of the transient multicomponent EM logging measurement is investigated ***,we adopt sine and cosine transform to calculate the transient multicomponent *** to the cosine transform,sine transform is more accurate in solving late-time ***,a time-domain geosignal is introduced to sense the *** show that DOD of this transient EM measurement can be up to tens of meters,including directionally ***,by studying the decay characteristics of different components with time,cross component is confirmed to decay much faster than the coaxial/coplanar components in the formation coordinate system.A pseudo-inversion is thereby proposed to determine the dip angle of anisotropic stratified *** simulation results indicate that this algebraic method can determine the true dip at some particular *** is still stable and valid even when considering random measurement ***,we establish the linear relationship between the time at which the half-point of geosignal curve appears and the distance to boundary(DTB),which would assist in the quick determination of DTB.

关键词： Time-domain Logging while drilling Formation boundary Dip angle Distance to boundary

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Face Detection under Complex Background and Illumination

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Journal of Electronic Science and Technology 2015年第1期13卷 78-82页

作者： shao-Dong Lv Yong-Duan Song mei Xu Cong-Ying Huang School of Energy Science and Engineering University of Electronic Science and Technology

For face detection under complex background and illumination, a detection method that combines the skin color segmentation and cost-sensitive Adaboost algorithm is proposed in this paper. First, by using the characteristic of human skin color clustering in the color space, the skin color area in YC b C r color space is extracted and a large number of irrelevant backgrounds are excluded; then for remedying the deficiencies of Adaboost algorithm, the cost-sensitive function is introduced into the Adaboost algorithm; finally the skin color segmentation and cost-sensitive Adaboost algorithm are combined for the face detection. Experimental results show that the proposed detection method has a higher detection rate and detection speed, which can more adapt to the actual field environment.

关键词： Adaboost cost-sensitive learning face detection skin color segmentation

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Phasing analysis of the transcriptome and epigenome in a rice hybrid reveals the inheritance and difference in DNA methylation and allelic transcription regulation

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Plant Communications 2021年第4期2卷 52-65页

作者： Jia-Wu Feng Yue Lu Lin shao Jianwei Zhang Huan Li ling-ling Chen National Key Laboratory of Crop Genetic Improvement College of InformaticsHuazhong Agricultural UniversityWuhan 430070China College of Life Science and Technology Guangxi UniversityNanning 530004China Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou UniversityYangzhou 225009China

Hybrids are always a focus of botanical research and have a high practical value in agricultural *** better understand allele regulation and differences in DNA methylation in hybrids,we developed a phasing pipeline for hybrid rice based on two parental genomes(PP2PG),which is applicable for Iso-Seq,RNA-Seq,and Bisulfite sequencing(BS-Seq).Using PP2PG,we analyzed differences in gene transcription,alternative splicing,and DNA methylation in an allele-specific manner between parents and progeny or different progeny *** phasing of Iso-Seq data provided a great advantage in separating the whole gene structure and producing a significantly higher separation ratio than *** interaction of hybrid alleles was studied by constructing an allele co-expression network that revealed the dominant allele effect in the *** expression variation between parents and the parental alleles in progeny showed tissue-or environment-specific patterns,which implied a preference for trans-acting regulation under different *** addition,by comparing allele-specific DNA methylation,we found that CG methylation was more likely to be inherited than CHG and CHHmethylation,and its enrichment in genic regions was connected to gene *** addition to an effective phasing pipeline,we also identified differentiation in OsWAK38 gene structure that may have led to the expansion of allele functions in *** summary,we developed a phasing pipeline and provided valuable insights into alternative splicing,interaction networks,trans-acting regulation,and the inheritance of DNA methylation in hybrid rice.

关键词： phasing hybrid trans-acting regulation allele co-expression network allele-specific expression allele-specific DNA methylation

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Effect of Surface State on Nodular Corrosion Resistance of Zircaloy-4 Alloy

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Acta Metallurgica Sinica(English Letters) 2015年第2期28卷 254-260页

作者： Chuan-Ming Chen Bang-Xin Zhou shao-Qiu Gou Jiao Huang mei-Yi Yao Laboratory for Microstructures Shanghai University Institute of Materials Shanghai University

The effect of surface state on the nodular corrosion resistance of Zircaloy-4 alloy was investigated in super- heated steam at 500 ℃/10.3 MPa by autoclave tests. The microstructures of oxide films on the corroded specimens were observed by TEM and SEM. The results indicate that surface strained layer delays the appearance of nodular spots on the specimen surfaces and improves the nodular corrosion resistance. The columnar grains orientation of the oxide films formed on the specimens with surface strained layer was more consistent than that on the specimens without surface strained layer when a comparison was made on the same orientation of the grain surfaces. Such a kind of oxide micro- structure formed on the specimens with surface strained layer can hinder the diffusion of oxygen ions along the grain boundaries and delay the growth of oxide films, therefore retard the formation process of nodular spots. This indicates that the microstructure of the initial oxide films has an important influence on the subsequent growth of the oxide films.

关键词： Zircaloy-4 Surface strained layer Nodular corrosion resistance Microstructures

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Fifty-five cases of hepatic alveolar echinococcosis combined with lymph node metastasis:A retrospective study

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World Journal of Gastroenterology 2024年第23期30卷 2981-2990页

作者： Yilizhati Aimaitijiang Tie-Min Jiang Ying-mei shao Tuerganaili Aji State Key Laboratory on Pathogenesis Prevention and Treatment of High Incidence Diseases in Central AsiaThe First Clinical CollegeXinjiang Medical UniversityUrumqi 830011Xinjiang Uygur Autonomous RegionChina The First Ward of Hepatobiliary and Pancreatic Surgery Tumor Hospital Affiliated to Xinjiang Medical UniversityUrumqi 830011Xinjiang Uygur Autonomous RegionChina Department of Hepatobiliary&Hydatid Diseases Digestive&Vascular Surgery CenterThe First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054Xinjiang Uygur Autonomous RegionChina Xinjiang Clinical Research Center for Echinococcosis and Hepatobiliary Diseases The First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054Xinjiang Uygur Autonomous RegionChina

BACKGROUND Lymph node metastasis is a specific type of metastasis in hepatic alveolar echinococcosis(AE).Currently,there is a scarcity of describing the clinical characteristics and lymph node metastasis rules of patients with hepatic AE combined with lymph node metastasis and its mechanism and management are still *** hepatectomy combined with regional lymph node dissection is a better *** To analyse the clinical features of hepatic AE combined with lymph node metastasis to explore its treatment and *** A total of 623 patients with hepatic AE admitted to the First Affiliated Hospital of Xinjiang Medical University from 1 January 2012 to 1 January 2022 were retrospectively ***-five patients with combined lymph node metastasis were analysed for their clinical data,diagnosis and treatment methods,follow-up efficacy,and characteristics of lymph node ***,we comparatively analysed the lymph node metastasis rates at different *** variables are expressed as frequencies and percentages,and the analysis of difference was performed using theχ2 *** Bonferroni method was used for pairwise comparisons when statistical differences existed between multiple categorical *** A lymph node metastasis rate of 8.8%(55/623)was reported in patients with hepatic AE,with a female predilection(69.1%)and a statistically significant sex difference(χ2=8.018,P=0.005).Of the 55 patients with lymph node metastasis,72.7%had a parasite lesion,neighbouring organ invasion,and metastasis stage of P3N1M0 and above,of which 67.3%,78.2%,and 34.5%of hepatic AE lesions invaded the bile ducts,blood vessels,and distant metastases,*** rates of lymph node metastasis of 16.4%,21.7%,and 34.2%were reported for a preoperative abdominal ultrasound,magnetic resonance imaging,and computed tomography *** patients were intraoperatively suspected with enlarged lymph nodes and underwent radica

关键词： Echinococcosis Radical hepatectomy Lymph node metastasis Lymph node dissection Alveolar echinococcosis Hepatic

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Oxide Inclusions in Ferromanganese and Its Influence on the Quality of Clean Steels

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Journal of Iron and Steel Research International 2014年第S1期21卷 23-27页

作者： Pei-wei HAN shao-jun CHU Ping mei Yi-fei LIN School of Metallurgical and Ecological Engineering University of Science and Technology Beijing Jiangsu Jiangnan Ferroalloy Co. Ltd.

Low and medium carbon ferromanganese produced by oxygen decarburization process and electric silicothermic process was briefly introduced, and the quality of products by these two processes was analyzed. Results showed that the total oxygen content in medium carbon ferromanganese by electric silicothermic process in China, which ranged from 0.039% to 0.171%, was between those of the common and refined products by oxygen decarburization process outside of China. The increments of total oxygen content in liquid steel were estimated when ferromanganese was added for the purpose of Mn element adjustment at the end of smelting. Refined low and medium carbon ferromanganese, which had low total oxygen content, was recommended for composition adjustment of clean steels during final stage of a heat. It is possible that the inclusions in the ferromanganese alloy greatly influenced the quality of clean steel indirectly by affecting the amount, size and composition of inclusions in steel.

关键词： low carbon ferromanganese medium carbon ferromanganese oxide inclusions clean steels

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Are bowel symptoms and psychosocial features different in irritable bowel syndrome patients with abdominal discomfort compared to abdominal pain?

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World Journal of Gastroenterology 2022年第33期28卷 4861-4874页

作者： Xiu-Cai Fang Wen-Juan Fan Douglas D Drossman shao-mei Han mei-Yun Ke Department of Gastroenterology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Gastroenterology Tongji HospitalTongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430030Hubei ProvinceChina Center of Functional GI and Motility Disorders University of North CarolinaChapel HillNC 27517United States Center for Education and Practice of Biopsychosocial Care Drossman GastroenterologyDurhamNC 27713United States Rome Foundation Rome FoundationRaleighNC 27614United States Department of Epidemiology and Statistics Institute of Basic Medical SciencesChinese Academy of Medical Sciences&School of Basic MedicinePeking Union Medical CollegeBeijing 100005China

BACKGROUND The Rome IV criteria eliminated abdominal discomfort for irritable bowel syndrome(IBS), which was previously included in Rome III. There are questions as to whether IBS patients with abdominal discomfort(seen in Rome III but not Rome IV) are different from those with abdominal pain(Rome IV).AIM To compare bowel symptoms and psychosocial features in IBS patients diagnosed with Rome III criteria with abdominal discomfort, abdominal pain, and pain &*** We studied IBS patients meeting Rome III criteria. We administered the IBS symptom questionnaire, psychological status, and IBS quality of life. Patients were classified according to the predominant abdominal symptom associated with defecation into an only pain group, only discomfort group, and pain & discomfort group. We compared bowel symptoms, extraintestinal symptoms, IBS quality of life, psychological status and healthcare-seeking behaviors, and efficacy among the three groups. Finally, we tested risk factors for symptom reporting in IBS *** Of the 367 Rome III IBS patients enrolled, 33.8%(124 cases) failed to meet Rome IV criteria for an IBS diagnosis. There were no meaningful differences between the pain group(n = 233) and the discomfort group(n = 83) for the following:(1) Frequency of defecatory abdominal pain or discomfort;(2) Bowel habits;(3) Coexisting extragastrointestinal pain;(4) Comorbid anxiety and depression;and(5) IBS quality of life scores except more patients in the discomfort group reported mild symptom than the pain group(22.9% vs 9.0%). There is a significant tendency for patients to report their defecatory and non-defecatory abdominal symptom as pain alone, or discomfort alone, or pain & discomfort(all P < 0.001).CONCLUSION IBS patients with abdominal discomfort have similar bowel symptoms and psychosocial features to those with abdominal pain. IBS symptoms manifesting abdominal pain or discomfort may primarily be due to different sensation and reporting expe

关键词： Irritable bowel syndrome Abdominal pain Abdominal discomfort Diagnosis Psychosocial distress Quality of life

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An experimental study on the velocity fluctuations generated by the flow past fixed spheres

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Journal of Hydrodynamics 2022年第2期34卷 214-225页

作者： ling-xin Zhang Xin-sheng Cheng Ze-cai Zhou Qi Gao Xue-ming shao State Key Laboratory of Fluid Power and Mechatronic Systems Department of MechanicsZhejiang UniversityHangzhou310027China

We perform a series of experiments to study the velocity fluctuations generated by the flow past fixed *** particle image velocimetry(PIV)is carried out to characterize the properties of the liquid *** fluctuation induced by the spheres can be decomposed into the temporal fluctuation and the spatial fluctuation,which represent the contribution of flow instability and spatial inhomogeneity,*** particularly,we focus on the contributions of temporal fluctuations and spatial fluctuations between low and high Reynolds *** low Reynolds number(20200,but not for Re<*** ending scale is aroundλ=0.5d and decreases slightly as Re increases.

关键词： Multiphase flow turbulence modulation particle image velocimetry(PIV)

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