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8-bromo-7-methoxychrysin inhibits properties of liver cancer stem cells via downregulation of β-catenin

World Journal of Gastroenterology 2013年第43期19卷 7680-7695页

作者： mei-fang quan Li-Hong Xiao Zhi-Hong Liu Hui Guo Kai-Qun Ren Fei Liu Jian-Guo Cao Xi-Yun Deng Medical College Hunan Normal University Department of pathology Hunan Provincial Tumor Hospital

AIM:To evaluate whether 8-bromo-7-methoxychrysin(BrMC),a synthetic analogue of chrysin,inhibits the properties of cancer stem cells derived from the human liver cancer MHCC97 cell line and to determine the potential ***:CD133+cells were sorted from the MHCC97 cell line by magnetic activated cell sorting,and amplified in stem cell-conditioned medium to obtain the enriched CD133+sphere forming cells(SFCs).The stem cell properties of CD133+SFCs were validated by the tumorsphere formation assay in vitro and the xenograft nude mouse model in vivo,and termed liver cancer stem cells(LCSCs).The effects of BrMC on LCSCs in vitro were evaluated by MTT assay,tumorsphere formation assay and transwell chamber *** effects of BrMC on LCSCs in vivo were determined using a primary and secondary xenograft model in Balb/c-nu *** of the stem cell markers,epithelialmesenchymal transition(EMT)markers andβ-catenin protein were analyzed by western blotting or immunohistochemical ***:CD133+SFCs exhibited stem-like cell properties of tumorsphere formation and tumorigenesis capacity in contrast to the parental MHCC97 *** found that BrMC preferentially inhibited proliferation and self-renewal of LCSCs(P<0.05).Furthermore,BrMC significantly suppressed EMT and invasion of ***,BrMC could efficaciously eliminate LCSCs in ***,we showed that BrMC decreased the expression ofβ-catenin in *** ofβ-catenin by small interfering RNA could synergize the inhibition of self-renewal of LCSCs induced by BrMC,while Wnt3a treatment antagonized the inhibitory effects of ***:BrMC can inhibit the functions and characteristics of LCSCs derived from the liver cancer MHCC97 cell line through downregulation ofβ-catenin expression.

关键词： Liver cancer Cancer stem cell 8-bromo7-methoxychrysin Self-renewal β-catenin

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7-difluoromethoxyl-5,4'-di-n-octylgenistein inhibits growth of gastric cancer cells through downregulating forkhead box M1

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World Journal of Gastroenterology 2012年第33期18卷 4618-4626页

作者： Hong-Lin Xiang Fei Liu mei-fang quan Jian-Guo Cao Yuan Lv Laboratory of Medical Engineering Medical College Hunan Normal University Changsha 410013 Hunan Province China

AIM: To investigate whether the 7-difluoromethoxyl-5, 4'-di-n-octylgenistein (DFOG), a novel synthetic genistein analogue, affects the growth of gastric cancer cells and its mechanisms. METHODS: A series of genistein analogues were prepared by difluoromethylation and alkylation, and human gastric cancer cell lines AGS and SGC-7901 cultured in vitro were treated with various concentrations of genistein and genistein analogues. The cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were incubated by DFOG at different concentrations. The growth inhibitory effects were evaluated using MTT and clonogenic assay. The distribution of the phase in cell cycle was analyzed using flow cytometric analysis with propidium iodide staining. The expression of the transcription factor forkhead box M1 (FOXM1) was analyzed by reverse transcription-polymerase chain reaction and Western blotting. The expression levelsof CDK1, Cdc25B, cyclin B and p27KIP1 protein were detected using Western blotting. RESULTS: Nine of the genistein analogues had more effective antitumor activity than genistein. Among the tested analogues, DFOG possessed the strongest activity against AGS and SGC-7901 cells in vitro. DFOG significantly inhibited the cell viability and colony formation of AGS and SGC-7901 cells. Moreover, DFOG efficaciously arrested the cell cycle in G2/M phase. DFOG decreased the expression of FOXM1 and its downstream genes, such as CDK1, Cdc25B, cyclin B, and increased p27KIP1 at protein levels. Knockdown of FOXM1 by small interfering RNA before DFOG treatment resulted in enhanced cell growth inhibition in AGS cells. Up-regulation of FOXM1 by cDNA transfection attenuated DFOG-induced cell growth inhibition in AGS cells. CONCLUSION: DFOG inhibits the growth of human gastric cancer cells by down-regulating the FOXM1 expression.

关键词： Gastric cancer 7-difluoromethoxyl-5 4'-din-octylgenistein Genistein Forkhead box M1 Therapeutic action

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“外出”矿工的子女教育存在问题研究——以江西安源G矿为例

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广西民族学院学报（哲学社会科学版） 2005年第3期27卷 63-66页

作者：梅方权中山大学人类学系

通过对江西安源G矿的田野调查,发现爷爷奶奶隔代抚养的模式越来越多,家庭教育与学校教育互动不够,一些学生出校门后变成“街角青年”,呼吁全社会关注并提出了相关建议。

关键词：外出矿工子女教育输出地

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综合农业现代化与湖南乡村都市化

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吉首大学学报（社会科学版） 2005年第2期26卷 74-78页

作者：周大鸣梅方权中山大学人类学系广东广州510275

在湖南推进工业化城市化的同时不能忽略农村的发展。根据湖南的实际情况,提出农村发展走综合农业现代化和乡村都市化的思路;同时讨论了推进综合农业现代化和乡村都市化的内涵和问题。

关键词：三农综合农业现代化乡村都市化湖南

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Computed tomography enterography-based radiomics for assessing mucosal healing in patients with small bowel Crohn's disease

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World Journal of Gastroenterology 2025年第3期31卷 62-72页

作者： Hao Ding Yuan-Yuan fang Wen-Jie Fan Chen-Yu Zhang Shao-Fei Wang Jing Hu Wei Han Qiao mei Department of Gastroenterology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Radiation Oncology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Gastroenterology Suzhou First People's HospitalSuzhou 234099Anhui ProvinceChina

BACKGROUND Mucosal healing(MH)is the major therapeutic target for Crohn's disease(CD).As the most commonly involved intestinal segment,small bowel(SB)assessment is crucial for CD ***,it poses a significant challenge due to its limited accessibility through conventional endoscopic *** To establish a noninvasive radiomic model based on computed tomography enterography(CTE)for MH assessment in SBCD *** Seventy-three patients diagnosed with SBCD were included and divided into a training cohort(n=55)and a test cohort(n=18).Radiomic features were obtained from CTE images to establish a radiomic *** demographics were analysed to establish a clinical model.A radiomic-clinical nomogram was constructed by combining significant clinical and radiomic *** diagnostic efficacy and clinical benefit were evaluated via receiver operating characteristic(ROC)curve analysis and decision curve analysis(DCA),*** Of the 73 patients enrolled,25 patients achieved *** radiomic-clinical nomogram had an area under the ROC curve of 0.961(95%confidence interval:0.886-1.000)in the training cohort and 0.958(0.877-1.000)in the test cohort and provided superior clinical benefit to either the clinical or radiomic models alone,as demonstrated by *** These results indicate that the CTE-based radiomic-clinical nomogram is a promising imaging biomarker for MH and serves as a potential noninvasive alternative to enteroscopy for MH assessment in SBCD patients.

关键词： Crohn’s disease Computed tomography enterography Mucosal healing Nomogram Radiomics

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中国海绵天然产物的研究

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天然产物研究与开发 2003年第4期15卷 359-363,368页

作者：薛松赵权宇张卫虞星炬金美芳袁权中国科学院大连化学物理研究所大连116023

据统计 ,从海洋生物的天然产物中获得药物或药物先导化合物的几率要比陆源生物高 ,海绵是海洋中除珊瑚以外的第二大生物资源。在海绵体内已发现许多高活性的化合物。中国海绵的研究主要集中在南海。南海海绵种类多、数量大 ,样品采集比... 详细信息

据统计 ,从海洋生物的天然产物中获得药物或药物先导化合物的几率要比陆源生物高 ,海绵是海洋中除珊瑚以外的第二大生物资源。在海绵体内已发现许多高活性的化合物。中国海绵的研究主要集中在南海。南海海绵种类多、数量大 ,样品采集比较容易。本文统计调查了国内外海绵研究的现状 ,重点综述中国已研究的海绵种类、所获得的天然化合物以及相关产物的药理研究结果 ,对上述研究的特点与存在的不足进行了分析、讨论。

关键词：中国海绵天然产物活性药理

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Ubiquitination in diabetes and its complications:A perspective from bibliometrics

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World Journal of Diabetes 2025年第1期16卷 187-203页

作者： Li-Yuan Xiong Wei Zhao Fa-quan Hu Xue-mei Zhou Yu-Jiao Zheng College of Traditional Chinese Medicine Anhui University of Chinese MedicineHefei 230012Anhui ProvinceChina

BACKGROUND Diabetes has a substantial impact on public health,highlighting the need for novel ***,an intracellular protein modification process,is emerging as a promising strategy for regulating pathological *** hypothesize that ubiquitination plays a critical role in the development and progression of diabetes and its complications,and that understanding these mechanisms can lead to new therapeutic *** To uncover the research trends and advances in diabetes ubiquitination and its complications,we conducted a bibliometric *** Studies on ubiquitination in diabetes mellitus and its complications were retrieved from the Web of Science Core *** mapping analysis was conducted using the CiteSpace *** We gathered 791 articles published over the past 23 years,focusing on ubiquitination in diabetes and its associated *** articles originated from 54 countries and 386 institutions,with China as the leading *** Jiao Tong University has the highest number of publications in this *** most prominent authors contributing to this research area include Wei-Hua Zhang,with Zhang Y being the most frequently cited ***,The Journal of Biological Chemistry is noted as the most cited in this *** predominant keywords included expression,activation,oxidative stress,phosphorylation,ubiquitination,degradation,and insulin *** The role of ubiquitination in diabetes and its complications,such as diabetic nephropathy and cardiomyopathy,is a key research ***,these areas require further investigations.

关键词： Diabetes mellitus Ubiquitination Bibliometric analysis CiteSpace Research trends

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Prevalence of Helicobacter pylori infection in Chinese military personnel:A cross-sectional,multicenter-based study

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World Journal of Gastroenterology 2025年第3期31卷 42-49页

作者： Han-Chen Min Chun-Yan Zhang fang-Yu Wang Xiao-Hui Yu Shan-Hong Tang Hong-Wu Zhu Ya-Gang Zhao Ji-Luo Liu Jian Wang Jing-Han Guo Xiao-mei Zhang Yun-Sheng Yang Chinese People’s Liberation Army Medical School Chinese People’s Liberation Army General HospitalBeijing 100853China Department of Gastroenterology and Hepatology The First Medical CenterChinese People’s Liberation Army General HospitalBeijing 100853China Sixth Healthcare Department The Second Medical CenterChinese People’s Liberation Army General HospitalBeijing 100853China Department of Gastroenterology The 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation ArmyLanzhou 730050Gansu ProvinceChina Department of Gastroenterology General Hospital of Western Theater CommandChengdu 610011Sichuan ProvinceChina Department of Gastroenterology General Hospital of Southern Theater CommandGuangzhou 510010Guangdong ProvinceChina Department of Epidemiology Naval Medical UniversityShanghai 200433China School of Medicine Nankai UniversityTianjin 300071China National Clinical Research Center for Geriatric Diseases Chinese People’s Liberation Army General HospitalBeijing 100853China

BACKGROUND Helicobacter pylori(***)infection is a prevalent disease encountered in military internal medicine and recognized as the main cause of dyspepsia,gastritis,and peptic ulcer,which are common diseases in military *** guidelines in China state all patients with evidence of active infection with *** are offered ***,the prevalence of *** infection and its regional distribution in the military population remain unclear,which hinders effective prevention and treatment *** the prevalence of *** infection in the military population will aid in the development of customized strategies to better manage this infectious *** To investigate the prevalence of *** infection in the Chinese military population in different geographic *** This multicenter,retrospective study included 22421 individuals from five tertiary hospitals located in north,east,southwest,and northwest cities of *** infection was identified using the urea breath test,which had been performed between January 2020 and December *** Of the 22421 military service members,7416(33.1%)were urea breath *** highest prevalence of *** was in the 30-39 years age group for military personnel,with an infection rate of 34.9%.The majority of infected subjects were younger than 40-years-old,accounting for 70.4%of the infected *** individuals serviced in Lanzhou and Chengdu showed a higher infection prevalence than those in Beijing,Nanjing,and Guangzhou,with prevalence rates of 44.3%,37.9%,29.0%,31.1%,and 32.3%,*** *** infection remains a common infectious disease among military personnel in China and has a relatively high prevalence rate in northwest China.

关键词： Helicobacter pylori Prevalence Military personnel Urea breath test Multicenter study

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Chronic toxicity and cytotoxicity of synthetic pyrethroid insecticide cis-bifenthrin

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Journal of Environmental Sciences 2009年第12期21卷 1710-1715页

作者： WANG Cui CHEN fang ZHANG quan fang Zhuo College of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310032 China College of Environmental Science and Engineering Zhejiang Gongshang University Hangzhou 310035 China

With the increasing use of synthetic pyrethroids （SPs）, the significance of ecological safety and health risk is an emerging concern, In this study, we evaluated the chronic aquatic toxicity of eis-bifenthrin （cis-BF） in Daphnia magna and its cytotoxicity in Chinese hamster ovary （CHO） cells as well as human cervical carcinoma （Hela） ceils. Chronic aquatic toxicity tests showed that cis-BF could significantly affect the reproduction of D. magna. The lowest observed effective concentration and the non-observed effective concentration of cis-BF to D. magna were 0.02 and 0.01 μg/L, respectively, and the chronic value was 0.014 μg/L. The intrinsic rate of natural increase was significantly decreased （p 〈 0.05） to 0.02 μg/L. The cytotoxicity assay demonstrated that cis-BF decreased cell viability in CHO and Hela cells in a concentration- and time-dependent manner. The IC50 values for Hela and CHO cells were 4.0 × 10^-5 and 3.2 × 10^-5 mol/L, respectively. Together, these results indicated that cis-BF induced chronic toxicity in both aquatic invertebrate animals and mammalian cells. These findings assist in understanding the impact of SPs on health and environmental safety. Considering the wide spectrum of SPs, a more comprehensive understanding of the negative effects is indispensible for planning future application and regulation of these pesticides.

关键词： cis-bifenthrin Daphnia magna chronic toxicity cytotoxicity

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Prediction and quantification of effective gas source rocks in a lacustrine basin:Western Depression in the Liaohe Subbasin,China

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Petroleum Science 2024年第4期21卷 2218-2239页

作者： Si-Bo Yang mei-Jun Li Hong Xiao fang-Zheng Wang Guo-Gang Cai Shuang-quan Huang State Key Laboratory of Petroleum Resources and Prospecting College of GeosciencesChina University of PetroleumBeijing102249China China University of Petroleum-Beijing at Karamay College of PetroleumKaramayXinjiang834000China Research Institute of Petroleum Exploration and Development Liaohe Oilfield CompanyPetroChinaPanjin124010LiaoningChina

Due to limited data on the geochemical properties of natural gas,estimations are needed for the effective gas source rock in evaluating gas ***,the pronounced heterogeneity of mudstones in lacustrine successions complicates the prediction of the presence and geochemical characteristics of gas source *** this paper,the Liaohe Subbasin of Northeast China is used as an example to construct a practical methodology for locating effective gas source rocks in typical lacustrine *** types of gas source rocks,microbial,oil-type,and coal-type,were distinguished according to the different genetic types of their natural gas.A practical three-dimensional geological model was developed,refined,and applied to determine the spatial distribution of the mudstones in the Western Depression of the Liaohe Subbasin and to describe the geochemical characteristics(the abundance,type,and maturation levels of the organic matter).Application of the model in the subbasin indicates that the sedimentary facies have led to heterogeneity in the mudstones,particularly with respect to organic matter *** effective gas source rock model constructed for the Western Depression shows that the upper sequence(SQ2)of the Fourth member(Mbr 4)of the Eocene Shahejie Formation(Fm)and the lower and middle sequences(SQ3 and SQ4)of the Third member(Mbr 3)form the principal gas-generating *** total volume of effective gas source rocks is estimated to be 586 km^(3).The effective microbial,oil-type,and coal-type gas source rocks are primarily found in the shallow western slope,the central sags,and the eastern slope of the Western Depression,*** study provides a practical approach for more accurately identifying the occurrence and geochemical characteristics of effective natural gas source rocks,enabling a precise quantitative estimation of natural gas reserves.

关键词： Effective gas source rock 3D geological modeling Spatial distribution Geochemical characteristics Lacustrine rift basin

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