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Fourier-domain optical coherence tomography-guided phototherapeutic keratectomy for the treatment of anterior corneal scarring

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International Journal of Ophthalmology(English edition) 2020年第11期13卷 1720-1726页

作者： Yu-Li Yang Qian Jian Bo Liu Ke Wang Yu-Juan Chen Lian Tan mei-Jun pu Yong Liu Southwest Hospital/Southwest Eye Hospital Third Military Medical University(Anny Medical University)Chongqing 400038China Chongqing Aier Mega Eye Hospital Aier Eye Hospital GroupChongqing 400060China

AIM:To evaluate the safety,visual and anatomic outcomes of fourier-domain optical coherence tomography(FD-OCT)-guided excimer laser phototherapeutic keratectomy(PTK)combined with photorefractive keratectomy(PRK)surgery in treating anterior corneal ***:Clinical data of 23 eyes of 21 patients with anterior corneal scarring underwent FD-OCT-guided PTK and PRK from Dec.2014 to Jul.2016 were *** were assessed for preoperative and postoperative uncorrected visual acuity(UCVA),best spectacle-corrected visual acuity(BSCVA),contrast sensitivity(CS),FD-OCT,corneal topography and colour figures of anterior ***:The preoperative corneal pathologic conditions included viral keratitis(7 patients,7 eyes),band keratopathy(2 patients,4 eyes),corneal dystrophy(4 patients,4 eyes),traumatic corneal disease(2 patients,2 eyes)and corneal chemical injury(6 patients,6 eyes).Mean follow-up time was 10.65(range,3-19)***(in IogMAR)improved from a mean of 0.79(95%Cl,0.281.29)preoperatively to a mean of 0.45(95%Cl,0.29-0.62)postoperatively(P=0.021).BSCVA(in IogMAR)improved from 0.57(95%Cl,0.27-0.88)preoperatively to a mean of 0.28(95%Cl,0.15-0.41)postoperatively(P=0.001).Corneal topographic indices postoperatively showed significant improvement in corneal cylinder(P=0.009),the surface regularity index(P=0.007)and surface asymmetry index(P=0.00).Postoperative spherical equivalent averaged-0.53 diopters(-1.49 to 0.42).No complications were associated with the ***:FD-OCT-guided PTK combined with PRK is safe and effective for the treatment of anterior corneal scarring by eliminating or reducing corneal opacities.

关键词： corneal scar phototherapeutic keratectomy fourier-domain optical coherence tomography corneal opacity corneal topographic indices

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Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits

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Journal of Integrative Plant Biology 2020年第8期62卷 1213-1226页

作者： Shi-Xin Zhou Yong Zhu Liang-Fang Wang Ya-Ping Zheng Jin-Feng Chen Ting-Ting Li Xue-mei Yang He Wang Xu-pu Li Xiao-Chun Ma Ji-Qun Zhao mei pu Hui Feng Yan Li Jing Fan Ji-Wei Zhang Yan-Yan Huang Wen-Ming Wang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China Rice Research Institute and Key Lab for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China

MicroRNAs(miRNAs)are known to fine-tune growth,development,and stress-induced ***-miR1873 is a rice-specific miRNA targeting LOC_***,we show that Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits via LOC_***-miR1873 was significantly upregulated in a susceptible accession but downregulated in a resistance accession at 24 h post-inoculation(hpi)of *** Osa-miR1873 enhanced susceptibility to *** and compromised induction of defense *** contrast,blocking Osa-miR1873 through target mimicry compromised susceptibility to *** and enhanced induction of defense *** expression of Osa-miR1873 also resulted in some defects in yield traits,including grain numbers and seed setting ***,overexpression of the target gene LOC_Os05g01790 increased rice blast disease resistance but severely penalized growth and *** together,we demonstrate that Osa-miR1873 fine-tunes the rice immunity-growth trade-off via LOC_Os05g01790,and blocking Osa-miR1873 could improve blast disease resistance without significant yield ***,the Osa-miR1873-LOC_Os05g01790 regulatory module is valuable in balancing yield traits and blast resistance.

关键词： miR187 immunity traits

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Osa-miR167d facilitates infection of Magnaporthe oryzae in rice

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Journal of Integrative Plant Biology 2020年第5期62卷 702-715页

作者： Zhi-Xue Zhao Qin Feng Xiao-Long Cao Yong Zhu He Wang Viswanathan Chandran Jing Fan Ji-Qun Zhao mei pu Yan Li Wen-Ming Wang Rice Research Institute and Key Lab for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China Department of Biotechnology Sree Narayana Guru CollegeCoimbatoreTamilnaduIndia

MicroRNAs(miRNAs)play important roles in rice response to Magnaporthe oryzae,the causative agent of rice blast *** the roles of rice miRNAs is of great significance for the disease ***-miR167d belongs to a conserved miRNA family targeting auxin responsive factor(ARF)genes that act in developmental and stress-induced ***,we show that OsamiR167d plays a negative role in rice immunity against *** by suppressing its target *** expression of Osa-miR167d was significantly suppressed in a resistant accession at and after 24 h post inoculation(hpi),however,its expression was significantly increased at 24 hpi in the susceptible accession upon *** *** rice lines over-expressing Osa-miR167d were highly susceptible to multiple blast fungal *** contrast,transgenic lines expressing a target mimicry to block OsamiR167d enhanced resistance to rice blast *** addition,knocking out the target gene ARF12 led to hypersusceptibility to multiple blast fungal *** together,our results indicate that Osa-miR167d negatively regulate rice immunity to facilitate the infection of *** by downregulating ***,Osa-miR167d-ARF12 regulatory module could be valuable in improvement of blast-disease resistance.

关键词： immunity expressing infection

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Effects of Non-specific and Specific Solvation on Adsorption of BPTI on Au Surface： Insight from Molecular Dynamics Simulation

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Chinese Journal of Chemical Physics 2013年第5期26卷 558-568,I0003,I0004页

作者： Wei Yang Li-yun Zhang Meng-long Li Xue-mei pu Nan-rong Zhao Faculty of Chemistry Sichuan University Chengdu 610064 China

Proteins adsorption at solid surfaces are of paramount important for many natural processes. However, the role of specific water in influencing the adsorption process has not been well understood. We used molecular dynamics simulation to study the adsorption of BPTI on Au surface in three water environments （dielectric constant model, partial and full solvation models）. The result shows that a fast and strong adsorption can occur in the dielectric environment, which leads to significant structure changes, as confirmed by great deviation from the crystal structure, largely spreading along the Au surface, rapid lose in all secondary structures and the great number of atoms in contact with the surface. Compared to the dielectric model, slower adsorption and fewer changes in the calculated properties above are observed in the partial solvation system since the specific water layer weakens the adsorption effects. However, in the partial solvation system, the adsorption of polar Au surface causes a significant decrease in the specific hydration around the protein, which still results in large structure changes similar to the dielectric system, but with much less adsorption extent. Enough water molecules in the full solvation system could allow the protein to rotate, and to large extent preserve the protein native structure, thus leading to the slowest and weakest adsorption. On the whole, the effects of non-specific and specific solvation on the protein structure and adsorption dynamics are significantly different, highlighting the importance of the specific water molecule in the protein adsorption.

关键词： Adsorption Au surface Implicit water Partial solvation Full solvation

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A K-means Optimized Clustering Algorithm Based on Improved Genetic Algorithm

A K-means Optimized Clustering Algorithm Based on Improved G...

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2021中国智能自动化大会(CIAC2021)

作者： Qiu-mei pu Qiong Wu Qian Li Minzu University of China

The K-means algorithm is highly sensitive to the initial clustering centers and easily get trapped in a local optimum. To avoid such problems, this paper proposes an improved crossover operator of chromosomes in the genetic algorithm, redefines the calculation method of genetic probability and the natural selection rules, introduces different individual selection mechanisms for the two adjacent generations of chromosomes, and integrates the K-means algorithm into the improved genetic algorithm. Experiment results demonstrate that the improved K-Means algorithm is better than the original genetic algorithm and KMeans algorithm in clustering performance, far better than the bisecting K-means algorithm.

关键词： Clustering K-means algorithm Genetic algorithm

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建立在固定样地上的林分生长线性联立方程组模型研究

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北京林业大学学报 2007年第1期29卷 37-41页

作者：郎璞玫中国林业科学研究院资源信息研究所林业遥感与信息技术实验室

为描述森林资源连续清查中两期固定样地因子之间的关系,该文分析了两期林分生长变量间的关系,以固定样地的两期(1990和1994年)观测变量为资料,在Borders模型基础上,引入标准地位指数和变量的线性变换,分优势树种构造出间隔期为5年的林... 详细信息

为描述森林资源连续清查中两期固定样地因子之间的关系,该文分析了两期林分生长变量间的关系,以固定样地的两期(1990和1994年)观测变量为资料,在Borders模型基础上,引入标准地位指数和变量的线性变换,分优势树种构造出间隔期为5年的林分生长线性联立方程组模型簇YB+xΓ=ε,并获得该模型待估参数矩阵B、Γ的无偏估计.该模型是可识别且误差结构矩阵不是对角矩阵的联立方程组模型.它表述了样地期末断面积和蓄积是林分标准地位指数、年龄、期初断面积的隐函数,较深入地描述了两期固定样地林分因子相互依存、从属的关系.通过模型的外检验,蓄积平均精度可达90%以上,林分纯生长量的估计精度可达80%以上.该模型具有较好的适应性,并可在今后若干个离散的时间点上预估同类林分的收获量,为研究在固定样地上两期林分自然生长规律提供较科学的理论和方法.

关键词：林分生长标准地位指数联立方程组离散化估计

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Osa-miR439 Negatively Regulates Rice Immunity Against Magnaporthe oryzae

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Rice science 2021年第2期28卷 156-165页

作者： LU Junhua YANG Xuemei CHEN Jinfeng LI Tingting HU Zijin XIE Ying LI Jinlu ZHAO Jiqun pu mei FENG Hui FAN Jing HUANG Yanyan ZHANG Jiwei WANG Wenming LI Yan State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China Rice Research Institute and Key Laboratory for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China

Osa-miR439 is a rice-specific microRNA family. Here we showed that Osa-miR439 acted as anegative regulator in rice immunity against blast fungus Magnaporthe oryzae. Osa-miR439 differentiallyresponded to M. oryzae between susceptible and resistant rice accessions. The accumulation ofOsa-miR439 was constitutively more in the susceptible accession than in the resistant one. Transgeniclines overexpressing Osa-miR439a (OX439a) showed higher susceptibility associating with lower inductionof defense-related genes and less hydrogen peroxide (H2O2) accumulation at the infection sites than thecontrol plants. In contrast, transgenic lines expressing a target mimic of Osa-miR439 (MIM439) displayedcompromised susceptibility associating with increased H2O2 accumulation. Furthermore, we found thatthe expression of three predicted target genes was decreased in OX439a but increased in MIM439 incomparison to control plants, and this expression was differential in susceptible and resistant accessionsupon M. oryzae infection, indicating that Osa-miR439a may regulate rice blast resistance via these *** results unveiled the role of Osa-miR439a in rice blast resistance and provided the potentiality toimprove the blast resistance via miRNA.

关键词： Osa-miR439 rice blast resistance target gene

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The rice miR171b–SCL6-IIs module controls blast resistance,grain yield,and flowering

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The Crop Journal 2022年第1期10卷 117-127页

作者： Yan Li Ying Tong Xiaorong He Yong Zhu Tingting Li Xiaoyu Lin Wei Mao Zeeshan Ghulam Nabi Gishkori Zhixue Zhao Jiwei Zhang Yanyan Huang mei pu Jing Fan Jing Wang Wenming Wang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina Rice Research Institute and Key Lab for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130SichuanChina

Micro RNAs(mi RNAs)act as regulators of plant development and multiple stress *** we demonstrate that the rice mi R171 b-SCL6-IIs module regulates the balance between blast resistance,grain yield,and *** R171 b-overexpressing rice plants(OX171 b)displayed increased rice blast resistance accompanied with enhanced defense responses and late heading,whereas blocking mi R171 b expression in rice(MIM171)led to greater susceptibility to blast disease,associated with compromised defense responses and early *** overexpressing or silencing of mi R171 b significantly affected plant height and number of filled seeds per panicle(seed-setting rate),resulting in decreased grain *** R171 b targets SCL6-IIa,SCL6-IIb,and SCL6-IIc,whose expression was suppressed in OX171 b but increased in *** of SCL6-IIa,SCL6-IIb,and SCL6-IIc all displayed phenotypes like that of OX171 b,including markedly increased blast disease resistance,slightly decreased grain yield,and delayed *** of mi R171 b increased gradually in leaves during the vegetative stage but decreased gradually in panicles during the reproductive stage,whereas SCL6-IIs displayed the reverse expression ***,these results suggest that the expression of mi R171 b was time-and space-dependent during the rice growth period and regulated the balance between rice blast disease resistance,grain yield,and flowering via SCL6-IIs,and that appropriate accumulation of mi R171 b is essential for rice development.

关键词： miR171b SCL6-IIs Blast disease resistance Yield trait Flowering

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0421号热带风暴“海马”登陆前后环境场对其移向和强度影响的分析

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台湾海峡 2005年第3期24卷 370-376页

作者：赵凯濮梅娟江苏省气象局江苏南京210008

0421号热带风暴“海马(Haima)”于2004年9月13日12:00在浙江省温州市沿海登陆,登陆后迅速减弱为热带低压,20:00在浙江境内消失.本研究采用物理量诊断分析法,探讨该热带风暴登陆前后涡度场、湿焓场变化对其移向和强度的影响.结果表明,该... 详细信息

0421号热带风暴“海马(Haima)”于2004年9月13日12:00在浙江省温州市沿海登陆,登陆后迅速减弱为热带低压,20:00在浙江境内消失.本研究采用物理量诊断分析法,探讨该热带风暴登陆前后涡度场、湿焓场变化对其移向和强度的影响.结果表明,该热带风暴登陆后,涡度(ξ)明显减弱.涡度局地变化(ξ/t)值分布显示,风暴移动方向的后方,涡度减弱的趋势比前方涡度增大的趋势更剧烈;风暴有向其外围(ξ/t)正值中心(即涡度增幅最大)移动的趋势.该热带风暴登陆前、后,湿焓都增大,但登陆后湿焓增大趋势已大大减弱;风暴有向湿焓局地变化(E/t)正值中心移动的趋势,即向能量增强最大的方向移动.

关键词：气象学移向和强度过程分析热带风暴涡度局地变化湿焓局地变化

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铼的弹性剪切参数C_(44)对温度与压力响应的原位拉曼光谱研究

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光谱学与光谱分析 2018年第12期38卷 3764-3768页

作者：戚磊雷力冯雷豪蒲梅芳四川大学原子与分子物理研究所四川成都610065

铼具有高体积弹性模量、高熔点、优良的抗蠕变性能,是一种重要科学研究与工程应用材料。铼的弹性剪切系数C_(44)对温度和压力的响应特性对铼及其合金材料的设计与工程应用具有重要意义。激光拉曼散射技术在研究不同温度和压力条件下六... 详细信息

铼具有高体积弹性模量、高熔点、优良的抗蠕变性能,是一种重要科学研究与工程应用材料。铼的弹性剪切系数C_(44)对温度和压力的响应特性对铼及其合金材料的设计与工程应用具有重要意义。激光拉曼散射技术在研究不同温度和压力条件下六方密堆积结构(hcp)金属的弹性剪切系数C_(44)上具有独特的优势。但由于金属的强反射作用和浅的穿透深度,hcp金属的低波数拉曼散射信号往往很难获取,在一定的温度和压力加载下拉曼信号的获取尤为困难。利用侧向激发拉曼散射技术,有效降低了金属强反射对拉曼光谱采集的影响,成功测量到多晶铼在不同压力与温度条件下的E2g拉曼振动模,获得铼在常温常压条件的弹性剪切系数(C_(44)=133 GPa)以及其弹性剪切系数C_(44)对温度和压力的响应特性。研究结果表明,多晶铼的弹性剪切系数C_(44)模量随压强的增加而增大,随温度的增加而减小。这也为用光散射方法研究金属材料剪切模量提供了良好研究方法。

关键词：铼拉曼光谱高温高压弹性剪切系数C44

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