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Risk assessment and genomic characterization of Zika virus in China and its surrounding areas

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Chinese Medical Journal 2019年第14期132卷 1645-1653页

作者： Rong-Fei Liu Zhen-Jian He peng mei Jia-Cheng Xi Xu-Dong Cao Li-Hong Yuan Jia-Hai Lu School of Public Health Sun Yat-sen UniversityGuangzhouGuangdong 510080China Key Laboratory of Tropical Disease Control(Sun Yat-sen University) Ministry of EducationGuangzhouGuangdong 510080China Department of Chemical and Biological Engineering University of OttawaOttawaOntarioCanada School of Life Sciences and Biopharmaceutics Guangdong Pharmaceutical UniversityGuangzhouGuangdong 510006China One Health Center of Excellence for Research & Training Sun Yat-sen UniversityGuangzhouGuangdong 510080China

Background:Zika virus (ZIKV) has emerged as a global pathogen causing significant public health *** has reported several imported cases where ZIKV were carried by travelers who frequently travel between China and ZIKV-endemic *** fully characterize the ZIKV strains isolated from the cases reported in China and assess the risk of ZIKV transmission in China,comprehensive phylogenetic and genetic analyses were performed both on all ZIKV sequences of China and on a group of scientifically selected ZIKV sequences reported in some of the top interested destinations for Chinese ***:ZIKV genomic sequences were retrieved from the National Center for Biotechnology Information database through stratified *** event detection,maximum likelihood (ML) phylogenetic analysis,molecular clock analysis,selection pressure analysis,and amino acid substitution analysis were used to reconstruct the epidemiology and molecular transmission of ***:The present study investigated 18 ZIKV sequences from China and 70 sequences from 16 selected *** events rarely happens in all ZIKV Asian *** genomes were generally undergone episodic positive selection (17 sites),and only one site was under pervasive positive *** ZIKV imported into China were Asian lineage and were assigned into two clusters:Venezuela-origin (cluster A) and Samoa-origin cluster (cluster B) with common ancestor from French *** time of most recent common ancestors of Cluster A dated to approximately 2013/11 (95% highest posterior density [HPD] 2013/06,2014/ 03) and cluster B dated to 2014/08 (95% HPD 2014/02,2015/01).Cluster B is more variable than Cluster A in comparison with other clusters,but no varied site of biological significance was *** strains in Southeast Asia countries are independent from strains in America ***:The genetic evolution of ZIKV is *** are two independent intro

关键词： Zika virus China Phylogeny Genetic evolution Risk assessment

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PHC桩的新型碗形端头开发及研制

水运工程 2009年第1期 143-148页

作者：史美鹏中交第三航务工程局有限公司宁波分公司浙江宁波315200

传统的PHC桩端头存在焊接质量控制困难,缺少质量检测方法;在焊接热影响区内会造成管桩混凝土的损伤;接头处刚度相对桩身来说较低等缺陷。阐述了新型碗形端头选型和理论值确定过程,分析了用碗形端头PHC桩的结构试验数据,并介绍工程实践情... 详细信息

传统的PHC桩端头存在焊接质量控制困难,缺少质量检测方法;在焊接热影响区内会造成管桩混凝土的损伤;接头处刚度相对桩身来说较低等缺陷。阐述了新型碗形端头选型和理论值确定过程,分析了用碗形端头PHC桩的结构试验数据,并介绍工程实践情况,证实了它的诸多优势。

关键词： PHC管桩端头焊接

Prognostic Factors for Patients with FIGO Stage-IB Cervical Squamous Cell Carcinoma:Does the Tumor Size(≤4 cm or>4 cm)Really Matter?

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Chinese Journal of Clinical Oncology 2007年第2期4卷 115-120页

作者： Ying Xiong Lizhi Liang Xiaoping peng mei Wei Yang Shen Department of Gynecologic Oncology Cancer Center Sun Yat-sen University Guangzhou 510060 China

OBJECTIVE To investigate the factors that can accurately predict the prognosis for patients with FIGO stage-IB cervical squamous cell carcinoma treated with radical surgery. METHODS A retrospective analysis of clinical data from 174 cases of FIGO Stage-IB cervical squamous cell carcinoma treated in our institute was conducted. RESULTS The 5-year overall disease-free survival of the patients was 79.4% and the recurrence rate was 16.7%. Seventy-five percent of the 60 patients with a tumor 〉 4 cm and 28.1% of the 114 patients with a tumor ≤ 4 cm received preoperative radiotherapy, resuting in a significant difference between the two groups （P 〈 0.001）. The 5-year disease-free survival rate for the groups with a tumor ≤ 4 cm without and with preoperative radiotherapy, and with a tumor 〉 4 cm without and with preoperative radiation therapy were 80.5%, 85.2%, 69.3% and 77.1%, respectively. There was no significant difference between any of the groups （P 〉 0.05）. A univariate analysis showed that pelvic node metastasis, a positive parametrial surgical margin and postoperative adjuvant therapy were all significantly correlated with the 5-year disease-free survivals （P 〈 0.05）. Multivariate analysis revealed that pelvic node metastasis （P = 0.004） and a positive parametrial surgical margin （P = 0.040） were independent factors that influenced the prognosis. The 5-year disease-free survivals for the cases with a tumor ≤ 4 cm and 〉 4 cm were 57.4% and 44.7% respectively in the high-risk group （patients with pelvic lymphatic metastasis and/or positive parametrial surgical margin）（P=0.575） and the recurrence ratio was 7/18 and 6/14 for the cases of the two tumor sizes in the same risk group. There was no significant difference between the two groups （P=0.821）. The 5-year disease-free survivals for the cases with a tumor ≤ 4 cm and 〉 4 cm were 86.5% and 82.9% respectively in the low-risk group （patients without pelvic lymph-node metastasis and/or positive parametrial surgical

关键词： cervical neoplasm tumor size prognosis

Efficacy of Traditional Chinese Medicine in the Treatment of Rash Caused by Epidermal Growth Factor Receptor Inhibitors: A Frequency Statistics and Meta-Analysis

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World Journal of Traditional Chinese Medicine 2019年第4期5卷 269-275页

作者： Shu-Yue Zheng Hui-Juan Cui Yan-mei peng Qiang Li Wen Shen Jing-Yi Zhang Chen-Yao Sun Xu Zhang Ke-Xin Tan Xue-Jiao Jiang Beijing University of Chinese Medicine China-Japan Friendship HospitalBeijingChina Department of Integrative Oncology China-Japan Friendship HospitalBeijingChina

Objective:The aim of this study is to evaluate the efficacy of Traditional Chinese Medicine(TCM)in the treatment of rash caused by epidermal growth factor receptor inhibitors(EGFRIs).Materials and Methods:Foreign language database(such as Pub Med,Web of Science,Cochrane Library,EMBASE)and Chinese language database(such as China National Knowledge Infrastructure,VIP Database for Chinese Technical Periodicals[VIP],Wangfang,CBM disc)were searched for all trials of TCM in the treatment of rash caused by EGFRIs until January of *** also looked through the references of relevant studies to supplement additional *** SPSS 25.0 was used for statistics of TCM with high frequency,and Review Manager 5.3 was used for ***:A total of 22 studies were included in the *** selected TCM whose frequency were>3.0%.They were Lonicera japonica(金银花),Licorice Roots Northwest Origin(生甘草),Cortex Dictamni(白鲜皮),Radix Sophorae Flavescentis(苦参),Schizonepeta(荆芥),Saposhnikovia Divaricate(防风).The meta-analysis revealed that the efficacy of TCM in treating EGFRIs-related rash was better than that of Western medicine or ***:TCM could significantly relieve rash caused by EGFRIs,which is worth ***,the mechanism deserves to be further explored.

关键词： Epidermal growth factor receptor inhibitors meta-analysis rash Traditional Chinese Medicine

Fe-2.5V合金渗碳钢板与20钢热压复合的研究

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材料热处理学报 2011年第S1期32卷 94-97页

作者：王殿梁李积武钟美鹏嘉兴学院南湖学院浙江嘉兴314001

研究了Fe-2.5V合金的渗碳及渗后合金与20钢的热压复合,结果表明,厚度为1 mm的Fe-2.5V合金,在温度950℃和碳势1.2%C条件下渗碳4 h,材料完全渗透,组织为屈氏体加V4C3碳化物。将渗碳后的Fe-2.5V合金与20钢热压复合,将发生碳原子自Fe-2.5V... 详细信息

研究了Fe-2.5V合金的渗碳及渗后合金与20钢的热压复合,结果表明,厚度为1 mm的Fe-2.5V合金,在温度950℃和碳势1.2%C条件下渗碳4 h,材料完全渗透,组织为屈氏体加V4C3碳化物。将渗碳后的Fe-2.5V合金与20钢热压复合,将发生碳原子自Fe-2.5V合金向20钢的扩散,碳的扩散深度为0.12 mm。界面附近的Fe-2.5V合金组织形貌未发生改变,而20钢的组织由原来的珠光体加铁素体转变为全珠光体组织。Fe-2.5V合金的硬度在1150 Hm左右。在距界面大约60μm处,硬度开始下降,在界面处的硬度为1100 Hm。

关键词： Fe-2.5V合金渗碳扩散热压复合

基质金属蛋白酶9作用后大肠癌细胞肿瘤坏死因子受体1的变化

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山东医药 2008年第16期48卷 3-5页

作者：彭梅白岚长沙市第八医院湖南长沙410001 南方医科大学南方医院

目的研究基质金属蛋白酶9(MMP-9)对大肠癌细胞肿瘤坏死因子受体1(TNFR1)表达的影响以及通过促进其脱落形成可溶性肿瘤坏死因子受体1(sTNFR1)从而促进大肠癌侵袭和转移的可能途径。方法免疫荧光法研究TNFR1在大肠癌细胞SW 1116中的表达;... 详细信息

目的研究基质金属蛋白酶9(MMP-9)对大肠癌细胞肿瘤坏死因子受体1(TNFR1)表达的影响以及通过促进其脱落形成可溶性肿瘤坏死因子受体1(sTNFR1)从而促进大肠癌侵袭和转移的可能途径。方法免疫荧光法研究TNFR1在大肠癌细胞SW 1116中的表达;用MMP-9干预培养的大肠癌SW 1116细胞,用流式细胞术检测MMP-9不同剂量和不同作用时间时TNFR1表达变化,同时应用双抗体夹心ELISA法检测细胞培养上清中sTN-FR1的变化。结果SW 1116细胞表达TNFR1;MMP-9使SW 1116细胞表面TNFR1脱落形成sTNFR1有剂量和时间依赖性。结论MMP-9促进大肠癌细胞表面TNFR1脱落形成sTNFR1,可能与大肠癌侵袭转移密切相关。

关键词：肠肿瘤基质金属蛋白酶肿瘤坏死因子受体1

羟基酪醇对脓毒症诱导的急性心肺损伤的保护作用及机制

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中国免疫学杂志 2019年第15期35卷 1814-1819,1824页

作者：石恩朋彭美玉德州市市立医院检验科德州253012 潍坊医学院临床医学院免疫学教研室潍坊261053

目的:探究羟基酪醇(HYD)对脓毒症诱导的急性心肺损伤的保护作用及机制。方法:盲肠结扎穿孔术构建小鼠脓毒症模型,HYD 20 mg/kg灌胃治疗。检测左心室收缩功能、心脏炎症因子mRNA和肺泡灌洗液炎症因子水平、肺损伤和中粒浸润程度、心肺组... 详细信息

目的:探究羟基酪醇(HYD)对脓毒症诱导的急性心肺损伤的保护作用及机制。方法:盲肠结扎穿孔术构建小鼠脓毒症模型,HYD 20 mg/kg灌胃治疗。检测左心室收缩功能、心脏炎症因子mRNA和肺泡灌洗液炎症因子水平、肺损伤和中粒浸润程度、心肺组织SIRT1和NF-κB水平、小鼠7 d生存率。结果:HYD升高左心室射血分数(EF)和左心室短轴缩短率(FS)(P<0.05),降低心脏和肺泡灌洗液炎症因子水平(P<0.01);改善肺损伤,减轻中性粒细胞浸润(P<0.01),延长生存率(P<0.01),HYD上调心肺组织SIRT1表达,抑制NF-κB通路活化。结论:HYD通过上调SIRT1和抑制NF-κB炎症信号通路而减轻脓毒症诱导的急性心肺损伤。

关键词：羟基酪醇脓毒症急性肺损伤心肌损伤 SIRT1 NF-κB

亲子沟通与同伴友谊质量对农村留守儿童社会适应性的影响

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中国特殊教育 2019年第9期 70-76页

作者：彭美戴斌荣青海师范大学教育学院西宁810008 盐城师范学院教育科学学院盐城224007

采用亲子沟通问卷、友谊质量问卷以及农村儿童社会适应问卷对958名农村学校儿童进行调查,建立结构模型,考察亲子沟通与同伴友谊质量对农村留守儿童社会适应性的影响。结果:(1)在亲子沟通方面,农村留守儿童母亲亲子沟通得分相对低于非留... 详细信息

采用亲子沟通问卷、友谊质量问卷以及农村儿童社会适应问卷对958名农村学校儿童进行调查,建立结构模型,考察亲子沟通与同伴友谊质量对农村留守儿童社会适应性的影响。结果:(1)在亲子沟通方面,农村留守儿童母亲亲子沟通得分相对低于非留守儿童,父亲亲子沟通得分上两者差异不显著;在同伴友谊质量方面,农村留守与非留守儿童得分差异不显著;在社会适应性方面,农村留守儿童得分相对低于非留守儿童。(2)亲子沟通、同伴友谊质量与农村留守儿童社会适应性两两之间均存在显著正相关。(3)同伴友谊质量在亲子沟通与农村留守儿童社会适应性之间起部分中介作用。

关键词：社会适应性亲子沟通同伴友谊质量农村留守儿童

鲍曼不动杆菌在环境美罗培南浓度变化时耐药性的改变及其机制

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上海交通大学学报（医学版） 2023年第11期43卷 1396-1407页

作者：赵富茂彭玫彭晓露舒韦韦彭丽重庆医科大学附属第一医院呼吸与危重症医学科重庆400016 重庆医科大学附属永川医院重症医学科重庆402160

目的探寻鲍曼不动杆菌(Acinetobacter baumannii)在环境中碳青霉烯类药物美罗培南浓度改变时耐药性变化的机制。方法通过改变鲍曼不动杆菌标准敏感株ATCC19606和临床耐药株AB.2014培养环境中的美罗培南浓度等条件,诱导对美罗培南不同耐... 详细信息

目的探寻鲍曼不动杆菌(Acinetobacter baumannii)在环境中碳青霉烯类药物美罗培南浓度改变时耐药性变化的机制。方法通过改变鲍曼不动杆菌标准敏感株ATCC19606和临床耐药株AB.2014培养环境中的美罗培南浓度等条件,诱导对美罗培南不同耐药程度的衍生株。测量所得菌株的生长曲线,并提取各菌株的DNA和RNA,采用PCR分析菌株耐药性改变后的碳青霉烯酶基因IMI、KPC、GES-1、IMP、VIM、NDM-1、OXA23、OXA24、OXA51、OXA58的表达情况;通过实时荧光定量PCR(real-time fluorescent quantitative PCR,RT-qPCR)分析不同耐药程度的鲍曼不动杆菌耐药基因,包括OXA51,外排泵基因adeB、adeG、adeJ,孔蛋白基因carO、omp33-36、oprC,青霉素结合蛋白基因ponA的表达水平变化;通过全基因组测序及生物信息学工具分析耐药性改变后菌株的差异基因富集情况的变化。结果获得了鲍曼不动杆菌ATCC19606与AB.2014对美罗培南不同耐药程度的11个衍生株,最低抑菌浓度(minimum inhibitory concentration,MIC)为1~128μg/mL。ATCC19606及其衍生株的生长速度和峰值随着耐药性的增加而降低,但AB.2014及其衍生株并没有表现出这种趋势。ATCC19606及其衍生株表达3个碳青霉烯酶基因OXA51、VIM和IMP,AB.2014及其多数衍生株表达4个碳青霉烯酶基因OXA23、OXA51、VIM和IMP,仅AB.2014的一个复敏衍生株出现了OXA23丢失。RT-qPCR结果显示,仅在ATCC19606及其耐药衍生株中oprC基因的表达量随着耐药性的升高而降低,多数耐药基因的表达水平与菌株的耐药水平变化一致。生物信息学分析提示ATCC19606不同衍生株之间的差异基因主要富集于铁载体摄取跨膜转运体活性、细胞外膜、细菌分泌系统和群体感应等,而AB.2014不同衍生株之间的差异基因主要富集于细胞外膜、细胞对化学刺激的反应、阿特拉津降解和RNA聚合酶等。结论碳青霉烯类药物环境压力会引起鲍曼不动杆菌耐药性发生变化,碳青霉烯酶、外排泵、孔蛋白、青霉素结合蛋白多种基因可能同时参与了菌株耐药性的变化;碳青霉烯酶OXA23丢失可能导致耐药鲍曼不动杆菌对碳青霉烯类药物复敏。

关键词：鲍曼不动杆菌美罗培南耐药性碳青霉烯酶外排泵孔蛋白青霉素结合蛋白