检索结果-南通市图书馆

Carrimycin inhibits coronavirus replication by decreasing the efficiency of programmed–1 ribosomal frameshifting through directly binding to the RNA pseudoknot of viral frameshift-stimulatory element

引用

Acta Pharmaceutica Sinica B 2024年第6期14卷 2567-2580页

作者： Hongying Li Jianrui Li Jiayu Li Hu Li Xuekai Wang Jing Jiang lei lei Han Sun mei Tang Biao Dong Weiqing He Shuyi Si Bin Hong Yinghong Li Danqing Song Zonggen Peng Yongsheng Che Jian-Dong Jiang CAMS Key Laboratory of Antiviral Drug Research Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Beijing Key Laboratory of Antimicrobial Agents Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Key Laboratory of Biotechnology of Antibiotics the National Health and Family Planning Commission(NHFPC)Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China

The pandemic of SARS-CoV-2 worldwide with successive emerging variants urgently calls for small-molecule oral drugs with broad-spectrum antiviral activity.Here,we show that carrimycin,a new macrolide antibiotic in the clinic and an antiviral candidate for SARS-CoV-2 in phase III trials,decreases the efficiency of programmed–1 ribosomal frameshifting of coronaviruses and thus impedes viral replication in a broad-spectrum fashion.Carrimycin binds directly to the coronaviral frameshift-stimulatory element(FSE)RNA pseudoknot,interrupting the viral protein translation switch from ORF1a to ORF1b and thereby reducing the level of the core components of the viral replication and transcription complexes.Combined carrimycin with known viral replicase inhibitors yielded a synergistic inhibitory effect on coronaviruses.Because the FSE mechanism is essential in all coronaviruses,carrimycin could be a new broad-spectrum antiviral drug for human coronaviruses by directly targeting the conserved coronaviral FSE RNA.This finding may open a new direction in antiviral drug discovery for coronavirus variants.

关键词： Carrimycin Coronavirus Broad-spectrum antiviral activity Programmed-1 ribosomal frameshifting RNA pseudoknot Antiviral agent RNA target Synergistic inhibitory effect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Novel lactylation-related signature to predict prognosis for pancreatic adenocarcinoma

引用

World Journal of Gastroenterology 2024年第19期30卷 2575-2602页

作者： Tian Peng Fang Sun Jia-Chun Yang mei-Hong Cai Man-Xiu Huai Jia-Xing Pan Fei-Yu Zhang lei-Ming Xu Department of Gastroenterology Xinhua Hospital Affiliated to Shanghai Jiao Tong UniversityShanghai 200092China

BACKGROUND Lactate,previously considered a metabolic byproduct,is pivotal in cancer progression and maintaining the immunosuppressive tumor microenvironment.Further investigations confirmed that lactate is a primary regulator,introducing recently described post-translational modifications of histone and non-histone proteins,termed lysine lactylation.Pancreatic adenocarcinomas are characterized by increased glycolysis and lactate accumulation.However,our understanding of lactylation-related genes in pancreatic adenocarcinomas remains limited.AIM To construct a novel lactylation-related gene signature to predict the survival of patients with pancreatic cancer.METHODS RNA-seq and clinical data of pancreatic adenocarcinoma(PDAC)were obtained from the GTEx(Genotype-Tissue Expression)and TCGA(The Cancer Genome Atlas)databases via Xena Explorer,and GSE62452 datasets from GEO.Data on lactylation-related genes were obtained from publicly available sources.Differential expressed genes(DEGs)were acquired by using R package“DESeq2”in R.Univariate COX regression analysis,LASSO Cox and multivariate Cox regressions were produced to construct the lactylation-related prognostic model.Further analyses,including functional enrichment,ESTIMATE,and CIBERSORT,were performed to analyze immune status and treatment responses in patients with pancreatic cancer.PDAC and normal human cell lines were subjected to western blot analysis under lactic acid intervention;two PDAC cell lines with the most pronounced lactylation were selected.Subsequently,RT-PCR was employed to assess the expression of LRGs genes;SLC16A1,which showed the highest expression,was selected for further investigation.SLC16A1-mediated lactylation was analyzed by immunofluorescence,lactate production analysis,colony formation,transwell,and wound healing assays to investigate its role in promoting the proliferation and migration of PDAC cells.In vivo validation was performed using an established tumor model.RESULTS In this study,we successfully identified 10 differentially e

关键词： Pancreatic adenocarcinoma Lactylation Prognosis Immunotherapy Tumor microenvironment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Arsenic uptake and transport of Pteris vittata L.as influenced by phosphate and inorganic arsenic species under sand culture

引用

Journal of Environmental Sciences 2007年第6期19卷 714-718页

作者： HUANG Ze-chun AN Zhi-zhuang CHEN Tong-bin lei mei XIAO Xi-yuan LIAO Xiao-yong Centre for Environmental Remediation Institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China

In order to understand the similarity or difference of inorganic As species uptake and transport related to phosphorus in Ashyperaccumulator, uptake and transport of arsenate （As（V）） and arsenite （As（Ⅲ）） were studied using Pteris vittata L. under sand culture. Higher concentrations of phosphate were found to inhibit accumulation of arsenate and arsenite in the fronds of P. vittata. The reduction in As accumulation was greater in old fronds than in young fronds, and relatively weak in root and rhizome. Moderate increases, from 0.05 to 0.3 mmol/L, in phosphate reduced uptake of As（Ⅲ） more than As（Ⅴ）, while the reverse was observed at high concentrations of phosphate （≥1.0 mmol/L）. Phosphate apparently reduced As transport and the proportion of As accumulated in fronds of P. vittata when As was supplied as As（Ⅴ）. It may in part be due to competition between phosphorus and As（Ⅴ） during transport. In contrast, phosphate had a much smaller effect on As transport when the As was supplied as As（Ⅲ）. Therefore, the results from present experiments indicates that a higher concentration of phosphate suppressed As accumulation and transport in P. vittata, especially in the fronds, when exposed to As（Ⅴ）, but the suppression of phosphate to As transport may be insignificant when P. vittata exposed to As（Ⅲ） under sand culture conditions. The finding will help to understand the interaction of P and As during their uptake process in P. vittata.

关键词： arsenate arsenite As species Pteris vittata L. phosphate transport uptake

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Silver Ion-Induced Formation of Unprecedented Thorium Nonamer Clusters via Lacuna-Construction Strategy

引用

CCS Chemistry 2023年第5期5卷 1144-1153页

作者： Xiang-He Kong Qun-Yan Wu lei mei Li-Wen Zeng Zhi-Wei Huang Ji-Pan Yu Chang-Ming Nie John KGibson Zhi-Fang Chai Kong-Qiu Hu Wei-Qun Shi Laboratory of Nuclear Energy Chemistry Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049 School of Chemistry and Chemical Engineering University of South ChinaHengyang 421001 Chemical Sciences Division Lawrence Berkeley National Laboratory(LBNL)BerkeleyCalifornia 94720

Herein,we report the synthesis and structures of two novel mixed-metal clusters denoted as Th_(9)Ag_(6)and Th_(9)Ag_(12).Both clusters feature unprecedented Th_(9)cores.The cores are tricapped trigonal prism moieties that are novel among actinides.Attempted alternative synthesis routes indicate that the Th_(9)clusters are accessible only through slow introduction of Ag_(+)into a solution containing a Th6 cluster modified with 2-picolinic acid.Alternative rapid addition of Ag_(+)leads to dissociation of the Th6 cluster with formation of a high-purity(ThAg)_(∞)two-dimensional layered structure material.A mechanism for cluster dissociation and reassembly to yield Th_(9)from Th6 is proposed that is consistent with spectroscopic observations and computational results.Because of Ag⋯Ag andπ–πinteractions,the Th_(9)Ag_(12)cluster exhibits high stability in air,at elevated temperature,underγ-irradiation,and in common solvents.

关键词： thorium nonamer clusters silver ion lacuna-construction strategy dissociation and reassembly high stability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A REPORT OF GROUND EXPERIMENTS FOR BRAIN FUNCTION IN SPACE

引用

空间科学学报 1991年第2期11卷 118-123页

作者： mei lei(Institute of Space Medico-Engineering, Beijing) 北京航天医学工程所北京

The brain function is of crucial importance in space adaptation and astronaut performance. According to the theory of nonequiliblium fluctuations a new technology, ET (Elec-troencephalofluctuography [EEFG] Technology), has been developed in our laboratory for space application. Information of different attracters, especially the dominant supra-slow (S) oscillations of the brain can be abstracted from the fluctuating brain waves. Specific S spectral lines related to neurophysiological and neuro-chemical activities have been identified and charcteristic spatial patterns have been figured out. ET analysis has been carried out in a series of ground experiments: (1) 20 degrees head lowered suspension experiments in rabbits; (2) 2Gz hypergravity experiments in rabbits; (3) effects of antimotionsickness drugs on rabbits during suspension; (4) clincal observations on patients with brain circulation diseases. Valuable information has been collected in all these experiments, which can hardly be obtained by means

关键词： Brain Brain waves Space adaptation Astronaut performance.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Characterization of three Arabidopsis AP2/EREBP family transcription factors involved in ABA sensitivity, freeze and salt tolerance

引用

Chinese Science Bulletin 2007年第13期52卷 1746-1753页

作者： mei WenQian lei Juan Xu Yu WEI Gang ZHU YuXian National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

AP2/EREBP transcription factors (TFs) play very important roles in plant development, hormonal regulation and stress response. Upon genome-wide cDNA cloning, phylogenetic and expression pattern analyses of this plant specific TF family, we found that three of the members including At1g71450, At1g50680 and At5g13910, were likely involved in responses to ABA, cold and salt. Complementary DNAs containing putative full-length ORFs of these three TFs were obtained and fused individually to the GAL4 DNA-binding domains. All the 3 genes functioned effectively as trans-activators using yeast one-hybrid assays. RT-PCR experiments showed that the At1g71450 gene was induced by ABA and low temperature; the At1g50680 gene was responsive to quite a few stress conditions, but especially to freezing temperature; and the At5g13910 gene was induced by high salt treatment, drought and ethyl-ene. By searching the ABRC T-DNA insertion mutant stocks, we obtained knockout lines for these TFs. Homozygous ko1 (At1g71450) plants showed a hypersensitive response to ABA during seed germina-tion and also in stomata movement. Homozygous ko2 (At1g50680) plants showed a significant reduc-tion in plant freezing tolerance compared to the wild type after chilling treatment. Homozygous ko3 (At5g13910) were less tolerant to high salinity than wild type plants. Our data suggest that At1g71450 is a negative regulator in ABA signaling, while At1g50680 and At5g13910 are positive regulators in cold and salt stress responses, respectively.

关键词：阿布属灵敏度土壤冻结盐碱地

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中国镉超富集植物的物种、生境特征和筛选建议

引用

环境科学 2023年第5期44卷 2786-2798页

作者：赵晓峰雷梅陈同斌中国科学院地理科学与资源研究所北京100101 中国科学院大学资源与环境学院北京100049

植物修复作为一项绿色且有效的原位修复重金属污染土壤技术,在近20年引起了中国学者的关注并取得了一系列成果.通过检索镉超富集植物相关关键词,对过去20 a(2002~2021年)科技文献中报道的中国镉超富集植物的物种特征、野外发现地在中国... 详细信息

植物修复作为一项绿色且有效的原位修复重金属污染土壤技术,在近20年引起了中国学者的关注并取得了一系列成果.通过检索镉超富集植物相关关键词,对过去20 a(2002~2021年)科技文献中报道的中国镉超富集植物的物种特征、野外发现地在中国各植被地带的分布、原生境特征、地质特征和镉的地球化学进行归纳总结,为镉超富集植物的筛选提供建议.结果表明,目前报道的镉超富集植物有45种.在植物物种上,隶属22科,36属,其中菊科(Compositae)植物最多,为14种.由野外调查发现的镉超富集植物有25种,主要发现于亚热带常绿阔叶林区域,其主要生长于铅锌矿周围的高浓度镉土壤上.归纳总结发现,丰富的植物物种资源、高浓度重金属土壤和长时间的驯化共同促进了超富集植物的形成.因此,具备这3点的区域可以视为超富集植物存在的高概率区域,并且超富集植物的野外筛选可以围绕此展开.最后,提出可以通过高概率区域识别和调查、富集能力验证、富集能力逆境习得筛查、生态型筛查、继代遗传检验和修复能力验证这6步,来进行超富集植物的筛选和植物超富集能力的鉴定.

关键词：超富集植物镉(Cd) 物种特征生境特征地理分布地质特征筛选

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Function of SeMNPV IAP3 in Mammalian Cells

引用

Virologica Sinica 2008年第3期23卷 183-188页

作者： Li-na ZHU Chun-lei mei Zhong-xin ZHANG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071China Graduate school Chinese Academy of Sciences Beijing 100039 China

The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I /Bam H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.

关键词：哺乳动物细胞 IAP3 基因分析方法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Downregulation of Serum PTEN Expression in Mercury-Exposed Population and PI3K/AKT Pathway-Induced Inflammation

引用

Biomedical and Environmental Sciences 2024年第4期37卷 354-366页

作者： mei Peng DING En Min YIN Hao Yang DING Xue Xue WANG Huan WANG Jian Feng HAN lei ZHANG Heng Dong ZHU Bao Li School of Public Health Nanjing Medical UniversityNanjing 210000JiangsuChina Institute of Occupational Disease Prevention Jiangsu Province Center for Disease Prevention and ControlNanjing 21000JiangsuChina Suzhou Center for Disease Prevention and Control Suzhou 215004JiangsuChina Center for Global Health School of Public HealthNanjing Medical UniversityNanjing 210000JiangsuChina

Objective This study investigated the impact of occupational mercury(Hg) exposure on human gene transcription and expression, and its potential biological mechanisms.Methods Differentially expressed genes related to Hg exposure were identified and validated using gene expression microarray analysis and extended validation. Hg-exposed cell models and PTEN lowexpression models were established in vitro using 293T cells. PTEN gene expression was assessed using qRT-PCR, and Western blotting was used to measure PTEN, AKT, and PI3K protein levels. IL-6 expression was determined by ELISA.Results Combined findings from gene expression microarray analysis, bioinformatics, and population expansion validation indicated significant downregulation of the PTEN gene in the high-concentration Hg exposure group. In the Hg-exposed cell model(25 and 10 μmol/L), a significant decrease in PTEN expression was observed, accompanied by a significant increase in PI3K, AKT, and IL-6 expression.Similarly, a low-expression cell model demonstrated that PTEN gene knockdown led to a significant decrease in PTEN protein expression and a substantial increase in PI3K, AKT, and IL-6 levels.Conclusion This is the first study to report that Hg exposure downregulates the PTEN gene, activates the PI3K/AKT regulatory pathway, and increases the expression of inflammatory factors, ultimately resulting in kidney inflammation.

关键词： PTEN Occupational mercury exposure Occupational health PI3K/AKT pathway 293T cell IL-6

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

轮缘推进器反厚度规律桨叶的翼型对比分析

引用

中国造船 2022年第1期63卷 113-125页

作者：杨蕾周军伟闫文辉梅蕾于东哈尔滨工业大学(威海)海洋工程学院威海264209 北方工业大学机械与材料工程学院北京100144

为了开发适用于轮缘推进器的无轮轴螺旋桨,以Ka4-70+19A导管螺旋桨为基础,构造了叶梢厚,叶根薄的反厚度规律桨叶,相应的桨叶翼型也进行了重新设计。根据不同的原则设计了3套桨叶翼型,使用CFX对3种桨叶进行敞水性能分析。结果表明:反厚... 详细信息

为了开发适用于轮缘推进器的无轮轴螺旋桨,以Ka4-70+19A导管螺旋桨为基础,构造了叶梢厚,叶根薄的反厚度规律桨叶,相应的桨叶翼型也进行了重新设计。根据不同的原则设计了3套桨叶翼型,使用CFX对3种桨叶进行敞水性能分析。结果表明:反厚度规律桨叶叶梢压力分布不均匀,吸力面靠近导边与随边位置出现较大面积高压区和压力最大值,压力面出现较大面积的低压区和压力最小值。Type1翼型梢部压力从导边到随边变化较大,敞水性能相对于原型桨变差;Type2桨叶能保持翼型攻角基本不变,但梢部载荷降低,敞水性能降低最多;Type3桨叶压力沿切向分布更均匀,敞水性能为三者最佳。研究结果可为RDT的反厚度螺旋桨设计提供参考。

关键词：轮缘推进器反厚度螺旋桨螺旋桨载荷桨叶设计

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：