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Polydatin attenuated food allergy via store-operated calcium channels in mast cell

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World Journal of Gastroenterology 2013年第25期19卷 3980-3989页

作者： Bo Yang jian-jie Li ji-Juan Cao Cheng-Bin Yang jie Liu Qiong-mei ji Zhi-Gang Liu Allergy Branch of the State Key Laboratory of Respiratory Disease School of Medicine Shenzhen University Liaoning Entry-Exit Inspection and Quarantine Bureau Department of Pathophysiology School of Medicine Shenzhen University

AIM: To investigate the effect of polydatin (PD), a resveratrol glucoside, on mast cell degranulation and antiallergic activity. METHODS: After the rats were orally sensitized with ovalbumin (OVA) for 48 d and underwent PD treatment for 4 d, all the rats were stimulated by 100 mg/mL OVA for24 h and then sacrificed for the following experiments. The small intestines from all the groups were prepared for morphology examination by hematoxylin and eosin staining. We also used a smooth muscle organ bath to evaluate the motility of the small intestines. The OVA-specific immunoglobulin E (IgE) production and interleu-kin-4 (IL-4) levels in serum or supernatant of intestinal mucosa homogenates were analyzed by enzyme-linked immunosorbent assay (ELISA). Using toluidine blue stain, the activation and degranulation of isolated rat peritoneal mast cells (RPMCs) were analyzed. Release of histamine from RPMCs was measured by ELISA, and regulation of PD on intracellular Ca 2+ mobilization was investigated by probing intracellular Ca 2+ with fluo-4 fluo-rescent dye, with the signal recorded and analyzed. RESULTS: We found that intragastric treatment with PD significantly reduced loss of mucosal barrier integrity in the small intestine. However, OVA-sensitization caused significant hyperactivity in the small intestine of allergic rats, which was attenuated by PD administration by 42% (1.26 ± 0.13 g vs OVA 2.18 ± 0.21 g, P < 0.01). PD therapy also inhibited IgE production (3.95 ± 0.53 ng/mL vs OVA 4.53 ± 0.52 ng/mL, P < 0.05) by suppressing the secretion of Th2-type cytokine, IL-4, by 34% (38.58 ± 4.41 pg/mLvs OVA 58.15 ± 6.24 pg/mL, P < 0.01). The ratio of degranulated mast cells, as indicated by vehicles (at least five) around the cells, dramatically increased in the OVA group by 5.5 fold (63.50% ± 15.51% vs phosphate-buffered saline 11.15% ± 8.26%, P < 0.001) and fell by 65% after PD treatment (21.95% ± 4.37% vs OVA 63.50% ± 15.51%, P < 0.001). PD mediated attenuation of mast cell

关键词： Polydatin Food allergy Mast cells Store-operated calcium channels Ca2+

DYNAMIC CHARACTERISTICS OF LIQUID FORCED SLOSHING IN A TURNING SPHERICAL TANK WITH A SPACER UNDER LOW GRAVITY

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Journal of Hydrodynamics 2008年第2期20卷 202-209页

作者： CHENG Xu-duo HU mei-zhu WEN ji-hua School of Food Science and Engineering Nanjing University of Finance and Economics Nanjing 210003China Department of Physics Anqing Teachers College Anging 246011 China

Under the condition of low gravity the characteristics of liquid forced sloshing in a turning spherical tank with a spacer were investigated. The static shape of the liquid surface was analyzed. By expanding the characteristic functions, the frequencies and velocity potential of liquid free-sloshing were derived. The governing equations and boundary conditions for the forced sloshing of liquid under the tank turning were established. The transverse force of liquid acting on the tank and the moment of force to the centre of the tank which is caused by the force of liquid acting on the spacer were given. Numerical results were compared with the ones of the spherical tank without a spacer. The results show that when a spacer is inserted in the tank, the sloshing frequency of liquid and the transverse force of liquid acting on the tank will decrease, but the moment of force to the centre of the tank which is caused by the force of liquid acting on the spacer will occur.

关键词： low gravity forced sloshing of liquid sloshing suppression

DYNAMIC CHARACTERISTICS OF LIQUID SLOSHING IN A TRANSVERSELY VIBRATING SPHERICAL TANK WITH A SPACER UNDER LOW GRAVITY

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Journal of Hydrodynamics 2008年第6期20卷 762-769页

作者： CHENG Xu-duo HU mei-zhu WEN ji-hua School of Food Science and Engineering Nanjing University of Finance and Economics Nanjing 210003 China Department of Physics Anqing Teachers College Anqing 246011 China

The dynamic characteristics of liquid sloshing in a transversely vibrating spherical tank with spacer under low gravity were investigated. By expanding the characteristic functions, the frequencies and velocity potentials of liquid free-sloshing were obtained. The dynamic equations and boundary conditions of liquid sloshing in the traverse vibrational spherical tank with spacer under low gravity were derived. By modifying the velocity potentials of liquid free-sloshing, the velocity potentials of liquid sloshing in the traverse vibrating spherical tank with a spacer were obtained. Furthermore, the forces and the moments acting on tank wall were given. The numerical results show when a spacer is inserted in the tank, the sloshing frequencies of liquid and the forces acting on tank wall will decrease, but the moment of force to the centre of the tank which is caused by the force of liquid acting on the spacer will occur.

关键词： low gravity liquid forced sloshing sloshing suppression

A gravity heat pipe for high voltage vacuum interrupter

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第9期10卷 1305-1312页

作者： Xiao-ling YU Zhi-yuan LIU Quan-ke FENG Yi-jiang WEI ji-mei WANG Xiang-jun ZENG School of Energy and Power Engineering Xi 'an Jiaotong University Xi 'an 710049 China School of Electrical Engineering Xi'an Jiaotong University Xi 'an 710049 China

To enhance nominal current of high voltage vacuum circuit breakers （VCBs）, a gravity heat pipe was proposed to replace stationary conducting rod of a high voltage vacuum interrupter. The heat pipe is composed of two coaxis tubes： the external tube is made of oxygen-free copper and the inner tube is made of stainless steel. The bottom end of the inner stainless steel tube is connected to the external copper tube by holes. Transient and static thermal performance of the heat pipe was measured, and the thermal resistance of it was compared with that of a solid copper rod with the same dimensions. Experimental results showed that thermal resistance of the heat pipe was about 1/3 of that of the copper rod, and it decreased slightly with the rising of the input heat flux. 3D thermal simulation on a 126 kV/2000 A single break VCB was done to compare the thermal performance between the proposed gravity heat pipe and the copper rod serving as the stationary conducting rod of the vacuum interrupter. Simulation results revealed that in the heat pipe case, the maximum temperature between contacts was 67 ℃ lower than that in the copper rod case.

关键词： Heat pipe Vacuum circuit breakers (VCBs) Vacuum interrupters Nominal current Thermal simulation

Optical Properties of Self-Organized PbS Quantum Dot Superlattices

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Chinese Physics Letters 2000年第10期17卷 755-756页

作者： YE Chang-Hui YAO Lian-Zeng MU ji-mei SHI Gang ZHANG Li-De Department of Materials Science and Engineering University of Science and Technology of ChinaHefei 230026 Institute of Solid State Physics Chinese Academy of SciencesHefei 230031

Self-organization of PbS into quantum dot superlattices has been demonstrated for the first time, and hexaplanar colloidal crystals 1 - 10 μm in size made from PbS quantum dots 3 - 6 nm in diameter are revealed in transmissionelectron microscope micrographs, and the inner structures of the superlattices can be seen by a high resolution transmission electron microscopy. The optical absorption and photoluminescence spectra have been recorded. The ordering of the superlattices is crucial for the understanding of the fundamental properties of quantum-dot arrays, as well as for their optimal utilization in optical and electronic applications.

关键词： quantum planar inner

A new polyketide synthase nonribosomal peptide synthetase hybrid metabolite from plant endophytic fungus Periconia sp.

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Chinese Chemical Letters 2016年第5期27卷 640-642页

作者： De-Wu Zhang Xiao-Yu Tao ji-mei Liu Ri-Dao Chen Min Zhang Xiao-mei Fang Li-Yan Yu Jun-Gui Dai State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College

A new polyketide synthase-nonribosomal peptide synthetase （PKS-NRPS） hybrid metabolite, named pericoannosin B （1）, was isolated from endophytic fungus Periconia sp. F-31 of the medicinal plant Annona rnuricata. The structure and absolute configuration were elucidated by means of extensive spectroscopic analyses （HRMS, NMR, IR, and CD）.

关键词： Endophytic fungus PericoniaPKS-NRPS Pericoannosin B

Two Crystal Phases of C3N4 Found in Carbon Nitride Films Prepared by Ion Implantation

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Chinese Physics Letters 1996年第10期13卷 782-785页

作者： GU You-song PAN Li-qing ZHAO Min-xue CHANG Xiang-rong TIAN Zhong-zhuo XIAO ji-mei Department of Material Physics and State Key Laboratory of New Metal MaterialsBeijing University of Sciences and TechnologyBeijing 100083

CNx films prepared by high dose ion implantation and post-annealing were studied by Auger electron spectroscopy,x-ray photoelectron spectroscopy and x-ray diffraction.A nearly uniform CNx layer was *** and nitrogen were found covalently bonded in the films.X-ray diffraction results showed that two crystal phases of C_(3)N_(4),β-C_(3)N_(4) and p-C_(3)N_(4),have been formed in the matrix of carbon.

关键词： diffraction. implantation spectroscopy

Effects of molybdenum on nutrition, quality, and flavour compounds of strawberry (Fragaria×ananassa Duch. Cv. Akihime) fruit

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Journal of Integrative Agriculture 2017年第7期16卷 1502-1512页

作者： LIU Li XIAO Wei ji mei-ling YANG Chao LI Ling GAO Dong-sheng FU Xi-ling State Key Laboratory of Crop Biology College of Horticulture Science and Engineering Shandong Agricultural University

Molybdenum （Mo） is an essential trace element in plant nutrition and physiology. It affects photosynthesis and photosyn- thate accumulation, therefore also affecting fruit quality and nutritional content. This study assessed the effects of different sodium molybdate （Na2MoO4） concentrations on strawberry. Five different Mo concentrations were applied in this experi- ment, including 0, 67.5, 135, 168.75, 202.5 g ha-1, respectively. The mineral concentration, including nitrogen （N）, Mo, iron （Fe）, copper （Cu）, and selenium （Se） was assessed in strawberry fruit, as well as chlorophyll content, nutrition quality, taste and aroma. Results showed that net photosynthetic rate （Pn） and chlorophyll content for the strawberry plants increased with an increase in Mo concentration; and the contents of N, Mo, Fe, Cu, total soluble solids （TSS）, titratable acidity （TA）, sweetness, some sugars, organic acids, and some volatile compounds in the fruit all increased, as well. However, the Mo concentration did not significantly affect the concentrations of Se, sucrose, lactic acid, acetic acid, and some aroma compounds. Fruit sprayed with 135 g ha-1 Mo exhibited the highest TSS and sweetness values, as well as the highest N and Fe concentrations among all the treatments. P value and chlorophyll content, fructose, glucose, sorbitol and total sugar contents in fruit supplied with 135 g ha-1 Mo were also higher than that in other treatments. Fruit sprayed with a Mo concentration of 67.5 g ha-1 exhibited significantly higher ascorbic acid （AsA） values than that of control. Ninety-seven volatile compounds were identified in fruit extracted by head-space solid-phase microextraction （HS-SPME） coupled with gas chromatography-mass spectrometry （GC-MS）. Fruits sprayed with 135 g ha-1 Mo had the highest concentrations of six characteristic aroma compounds, including methyl butanoate, y-decalactone, ethyl butanoate, methyl hexanoate, y-do- decalactone, and ethyl caproate.

关键词： strawberry molybdenum nutrition volatile compounds flavour

Systematical regulation involved in heterogeneous photosynthetic characteristics of individual leaf in pima cotton

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Journal of Integrative Agriculture 2022年第4期21卷 995-1003页

作者： ZHANG Yu-jie HAN ji-mei LEI Zhang-ying MENG Hao-feng ZHANG Wang-feng ZHANG Ya-li Key Laboratory of Oasis Eco-Agriculture Xinjiang Production and Construction Corps/College of AgronomyShihezi UniversityShihezi 832003P.R.China

Light heterogeneity leads to anatomically and physiologically heterogeneous features in leaves. However, little attention has been paid to the effects of nonuniform illumination on the anatomical and photosynthetic performance on both sides along the leaf main vein. This study explored such effects by combining in situ determination in the field with shading simulation in the phytotron, on pima cotton that has cupping leaves. Photosynthetic characteristics and morphological structures were measured in the field on both sides along the main vein of eastward, westward, southward, and northward leaves. The results showed that the difference in photosynthetic capacity between the two sides along the main vein in different directions was closely related to the daily photo irridiance(DPI). This result indicates that the photosynthetic heterogeneity between the two sides is related to their intercepted light energy. The conclusion was further verified by the shading simulation experiments. Photosynthetic capacity and leaf thickness of the unshaded sides of leaves in the half-shaded treatment decreased, compared to those in the unshaded treatment. Therefore, it is conjectured that the development of photosynthetic characteristics on one side is systematically regulated by that on the other side. The study provides theoretical guidance on accessing the feasibility of sampling and directional planting.

关键词： photosynthetic heterogeneity morphological traits leaf mass per area shade systemic regulation