检索结果-南通市图书馆

Mitochondrial dysfunction and cellularmetabolic deficiency in Alzheimer's disease

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2012年第5期28卷 631-640页

作者： Xue-mei gu Han-Chang Huang Zhao-Feng Jiang Beijing Military General Hospital Beijing 100700 China Beijing Key Laboratory of Bioactive Substances and Functional Foods Beijing Union University Beijing 100191 China College of Life Science and Technology Beijing University of Chemical Technology Beijing 100029 China

Alzheimer＇s disease （AD） is an age-related neurodegenerative disorder. The pathology of AD includes amyloid-β （Aβ） deposits in neuritic plaques and neurofibrillary tangles composed of hyperphosphorylated tau, as well as neuronal loss in specific brain regions. Increasing epidemiological and functional neuroimaging evidence indicates that global and regional disruptions in brain metabolism are involved in the pathogenesis of this disease. Aβ precursor protein is cleaved to produce both extracellular and intracellular Aβ, accumulation of which might interfere with the homeostasis of cellular metabolism. Mitochondria are highly dynamic organelles that not only supply the main energy to the cell but also regulate apoptosis. Mitochondrial dysfunction might contribute to Aβ neurotoxicity. In this review, we summarize the pathways ofAβ generation and its potential neurotoxic effects on cellular metabolism and mitochondrial dysfunction.

关键词： Alzheimer’s disease amyloid-β metabolic deficiency mitochondrial dysfunction

Surf4,cargo trafficking,lipid metabolism,and therapeutic implications

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2022年第9期14卷 3-13页

作者： Yishi Shen Hong-mei gu Shucun Qin Da-Wei Zhang Group on the Molecular and Cell Biology of Lipids and Department of Pediatrics Faculty of Medicine and DentistryUniversity of AlbertaEdmonton ABT6R2G3Canada Institute of Atherosclerosis in Shandong First Medical University(Shandong Academy of Medical Sciences) Taian 271016China

Surfeit 4 is a polytopic transmembrane protein that primarily resides in the endoplasmic reticulum(ER)*** is ubiquitously expressed and functions as a cargo receptor,mediating cargo transport from the ER to the Golgi apparatus via the canonical coat protein complex Il(CoPll)-coated vesicles or specific *** also participates in ER-Golgi protein trafficking through a tubular ***,it facilitates retrograde transportation of cargos from the Golgi apparatus to the ER through Coplcoated ***4 can selectively mediate export of diverse cargos,such as PCSK9 very low-density lipoprotein(VLDL),progranulin,α1-antitrypsin,STING,proinsulin,and *** has been implicated in facilitating VLDL secretion,promoting cell proliferation and migration,and increasing replication of positive-strand RNA ***,Surf4 plays a crucial role in various physiological and pathophysiological processes and emerges as a promising therapeutic ***,the molecular mechanisms by which Surf4 selectively sorts diverse cargos for ER-Golgi protein trafficking remain ***,we summarize the most recent advances in Surf4,focusing on its role in lipid metabolism.

关键词： VLDL secretion cargo receptor PCSK9 lipid metabolism atherosclerosis trafficking

Hypercholesterolemia,low density lipoprotein receptor and proprotein convertase subtilisin/kexin-type 9

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2015年第5期29卷 356-361页

作者： Hong-mei gu Da-wei Zhang Departments of Pediatrics and Biochemistry Group on the Molecular and Cell Biology of LipidsUniversity of Alberta

Atherosclerotic cardiovascular disease is the main cause of mortality and morbidity in the world. Plasma levels of low density lipoprotein cholesterol （LDL-C） are positively correlated with the risk of atherosclerosis. High plasma LDL concentrations in patients with hypercholesterolemia lead to build-up of LDL in the inner walls of the arteries, which becomes oxidized and promotes the formation of foam cells, consequently initiating atherosclerosis. Plasma LDL is mainly cleared through the LDL receptor （LDLR） pathway. Mutations in the LDLR cause familiar hyperch- olesterolemia and increase the risk of premature coronary heart disease. The expression of LDLR is regulated at the transcriptional level via the sterol regulatory element binding protein 2 （SREBP-2） and at the posttranslational levels mainly through proprotein convertase subtilisin/kexin-type 9 （PCSK9） and inducible degrader of the LDLR （IDOL）. In this review, we summarize the latest advances in the studies of PCSK9.

关键词： hypercholesterolemia low density lipoprotein receptor statin atherosclerosis proprotein convertasesubtilisin/kexin -type 9

The Synthesis and Characterization of High Molecular Weight Poly(phenylene sulfide/ether)

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第10期17卷 1373-1376页

作者： Ai Qun gu mei Ju XIE Zi Li YU Institute of Materials Science ＆ Technology Analytical ＆ Testing Center Sichuan University Chengdu 610064

Poly（phenylene sulfide/ether）（PPSE） was synthesized from 4,4＇-dihydroxydiphenyl sulfide and 4,4＇-dichlorodiphenyl sulfide in solution by nucleophilic substitution reaction. The resulting polymer was characterized by viscosity measurement, elemental analysis, FT-IR, ^1H NMR, X-ray diffraction and thermal analysis. The results showed that the viscosities of the resulting polymer were above 0.68 dL/g, and the linear chain structure of product was confirmed. PPSE had the same reflex indices as poly（p-phenylene sulfide）, an orthorhombic crystalline with unit cell a=0.853, b=0.562, c=1.026nm. The melting temperature, glass transition temperature and initial decomposition temperature were found to be 228℃, 85℃ and 325℃, respectively. The product was soluble in common organic solvents such as NMP, N, N＇-dimethylformamide, N, N＇-dimethylacetamide and 1,2-dichloroethane.

关键词： Poly(phenylene sulfide/ether) (PPSE) synthesis characterization.

Dickkopf3 overexpression inhibits pancreatic cancer cell growth in vitro

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第33期17卷 3810-3817页

作者： Yu-mei gu Yi-Hui Ma Wu-Gan Zhao Jie Chen Department of Pathology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

AIM:To elucidate the role of dickkopf3(Dkk3)in human pancreatic cancer cell ***:Dkk3 mRNA and protein expression in human pancreatic cancer cell lines were detected by realtime reverse transcription polymerase chain reaction(realtime RTPCR),Western blotting and *** of the Dkk3 promoter sequence was examined by methylationspecific polymerase chain reaction(MSP)and Dkk3 mRNA expression was determined by realtime RTPCR after 5aza2'deoxycytidine(5azadC)*** effects of Dkk3 on cancer cell proliferation and in vitro sensitivity to gemcitabine were investigated by CellTiter 96?AQueous One Solution Cell Proliferation Assay(MTS)after transfecting the Dkk3 expression plasmid into human pancreatic cancer *** expression ofβcatenin,phosphorylated extracellular signalregulated protein kinases(pERK)and extracellular signalregulated protein kinases(ERK)was also examined by realtime RTPCR and Western blotting after upregulating Dkk3 expression in human pancreatic cancer ***:The results show that the expression levels of both Dkk3 mRNA and protein were low in all pancreatic cancer cell lines *** Dkk3 promoter sequence was methylated in the MIA PaCa2 and AsPC1 cell lines,which showed reduced Dkk3 *** two cell lines,which initially had a methylated Dkk3 promoter,showed increased Dkk3 mRNA expression that was dependent upon the dosage and timing of the DNA demethylating agent,5azadC,treatment(Pgulated following the transfection of a Dkk3 expression plasmid into MIA PaCa2 cells,the ability of cells to proliferate decreased(Pgulated(Pgulation ofβcatenin expression via the ERKmediated pathway.

关键词： Cell growth Dickkopf3 In vitro Overexpression Pancreatic cancer

Study on the reform of resource tax and sustainable development of the underdeveloped resource-rich regions in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ecological Economy 2007年第3期3卷 266-275页

作者： Xie mei＇e gu Shuzhong Institute of Geographic Science and Natural Resources Research Chinese Academy of SciencesBeijing 100101.P.R.China

The underdeveloped resource-rich region in China refers to the underdeveloped region with abundantnatural resources. The problem for these regions sustainable development cannot be negligible for its particularity innatural resources and environment. This paper mainly examines the issue about resource tax reform and the sustainabledevelopment of underdeveloped resource-rich regions. It first expatiates on regional characteristics of underdevelopedresource-rich regions, then analyzes the effect that resource tax has on the sustainable development of this type of regions,takes Yulin City as example to illuminate the negative influence on the sustainable development of those regions due to thedesign limitation of the existing resource tax, and put forward some suggestions, including to delegate tax legislativepower to lower levels, to increase resource tax rate, to expand its levied scope to give play to the potential main tax to localrevenue, to improve the design of resource tax, to enhance the its function of resource saving and environmentalprotection, to attach importance to regional interest of underdeveloped resource-rich regions and to improve of theinterest distribution system concerning the tax system and resource exploitation.

关键词： Resource tax Reform Underdeveipled regions Resource-rich regions Sustainable development

Electrospun and woven silk fibroin/poly(lactic-coglycolic acid) nerve guidance conduits for repairing peripheral nerve injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2015年第10期10卷 1635-1642页

作者： Ya-ling Wang Xiao-mei gu Yan Kong Qi-lin Feng Yu-min Yang Key Laboratory of Eco-Textiles Ministry of Education Jiangnan University School of Chemistry and Chemical Engineering Nantong University Jiangsu College of Engineering and Technology Jiangsu Key Laboratory of Neuroregeneration Nantong University School of Medicine Nantong University

We have designed a novel nerve guidance conduit（NGC） made from silk fibroin and poly（lactic-co-glycolic acid） through electrospinning and weaving（ESP-NGCs）. Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application.

关键词： nerve regeneration peripheral nerve injury poly(lactic-co-glycolic acid) electrospinning silk fibroin biocompatibility nerve guidance conduit weaving

Predictors of Adverse Pregnancy Outcomes Following Traumatic Injuries

在线全文

学校读者我要写书评

暂无评论

Current Medical Science 2024年第3期44卷 642-647页

作者： Wan-rong LU Ping WU Gong SONG mei-qi gu Zhe XU Li HE National Clinical Research Center for Gynecology and Obstetrics Cancer Biology Research CenterTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Traumatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective After traumatic injury in pregnant women,providing timely and appropriate management for high-risk patients is crucial for both pregnant women and *** study aimed to identify risk factors that predict adverse pregnancy outcomes after traumatic *** A retrospective cohort study including 317 pregnant patients who experienced trauma was *** collected data included general demographics,injury mechanisms and adverse pregnancy *** were divided into two subgroups based on the absence or presence of trauma-related adverse pregnancy *** and multivariate logistic regressions were conducted to estimate the associations between clinical variables and adverse pregnancy *** A total of 41(12.93%)patients experienced adverse pregnancy outcomes within the first 24 h *** study revealed that age>35 years(OR=14.995,95%CI:5.024–44.755,P<0.001),third trimester trauma(OR=3.878,95%CI:1.343–11.204,P=0.012),abdominal pain(OR=3.032,95%CI:1.221–7.527,P=0.017),vaginal bleeding(OR=3.226,95%CI:1.093–9.523,P=0.034),positive scan in focused assessment with sonography for trauma(FAST)positive(OR=8.496,95%CI:2.825–25.555,P<0.001),9≤injury severity score(ISS)<16(OR=3.039,95%CI:1.046–8.835,P=0.041)and ISS≥16(OR=5.553,95%CI:1.387–22.225,P=0.015)increased the probability of posttraumatic adverse pregnancy *** age,gestational age at delivery,vaginal bleeding and positive FAST results were risk factors for abnormal *** Advanced maternal age,third trimester,and positive FAST results should alert multidisciplinary trauma teams to closely monitor patients to prevent adverse pregnancy outcomes.

关键词： adverse pregnancy outcomes predictive factors abnormal delivery trauma

Pediatric temporal fistula:Report of three cases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2021年第26期9卷 7811-7817页

作者： mei-Zhen gu Hong-Ming Xu Fang Chen Wei-Wei Xia Xiao-Yan Li Department of Otorhinolaryngology-Head and Neck Surgery Shanghai Children's HospitalShanghai Jiaotong UniversityShanghai 200062China Department of Radiology Shanghai Children's HospitalShanghai Jiaotong UniversityShanghai 200062China

BACKGROUND Pediatric temporal fistulae are rarely reported in the *** of these cases can help inform future diagnosis and effective *** SUMMARY Three pediatric patients came to the clinic due to repeated infections of the skin and soft tissue of the temporal *** patient presented with a temporal fistula that penetrated the temporal bone and reached the dura *** patient presented with a temporal fistula that penetrated into the temporal muscle *** third patient presented with a fistula that penetrated the lateral wall of the orbit and entered the *** patients underwent surgical fistula resection informed by preoperative computed tomography(CT)*** evaluation was also *** three patients were surgically treated *** evaluations confirmed the fistula diagnoses in all three *** For patients who have temporal fistulae with repeated infections,surgical treatment should be performed as soon as possible to prevent serious *** can be very useful for preoperative evaluation.B-mode ultrasound examination and evaluation also have a certain auxiliary role.

关键词： Temporal fistula Surgical treatment Pediatric Surgery Infection Congenital Case report