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Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice

Plant Communications 2024年第2期5卷 67-78页

作者： Ajay Gupta Bo Liu saad Raza Qi-Jun Chen Bing Yang Division of Plant Science and Technology Bond Life Sciences CenterUniversity of MissouriColumbiaMO 65211USA State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China Donald Danforth Plant Science Center St.LouisMO 63132USA

Prime editing(PE)technology enables precise alterations in the genetic code of a genome of *** offers great potential for identifying major agronomically important genes in plants and editing them into superior variants,ideally targeting multiple loci simultaneously to realize the collective effects of the ***,we report the development of a modular assembly-based multiplex PE system in rice and demon-strate its efficacy in editing up to four genes in a single transformation *** duplex PE(DPE)system achieved a co-editing efficiency of 46.1%in the T0 generation,converting TFIIAg5 to xa5 and xa23 to *** resulting double-mutant lines exhibited robust broad-spectrum resistance against multiple Xanthomonas oryzae pathovar oryzae(Xoo)strains in the T1 *** addition,we success-fully edited OsEPSPS1 to an herbicide-tolerant variant and OsSWEET11a to a Xoo-resistant allele,achieving a co-editing rate of 57.14%.Furthermore,with the quadruple PE(QPE)system,we edited four genes—two for herbicide tolerance(OsEPSPS1 and OsALS1)and two for Xoo resistance(TFIIAg5 and OsSWEET11a)—using one construct,with a co-editing efficiency of 43.5%for all four genes in the T0 *** performed multiplex PE usingfive more constructs,including two for triplex PE(TPE)and three for QPE,each targeting a different set of *** editing rates were dependent on the activity of pegRNA and/or *** instance,optimization of ngRNA increased the PE rates for one of the targets(OsSPL13)from 0%to 30%but did not improve editing at another target(OsGS2).Overall,our modular assembly-based system yielded high PE rates and streamlined the cloning of PE reagents,making it feasible for more labs to utilize PE for their editing *** have significant implications for advancing gene editing techniques in plants and may pave the way for future agricultural applications.

关键词： prime editing rice bacterial blight herbicide tolerance multiplex genome editing

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Improving the Transmission Security of Vein Images Using a Bezier Curve and Long Short-Term Memory

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Computers, Materials & Continua 2024年第6期79卷 4825-4844页

作者： Ahmed H.Alhadethi Ikram Smaoui Ahmed Fakhfakh saad M.Darwish Computer Science and School of Electronics and Telecommunications University of SfaxSfax3029Tunisia LETI Laboratory University of SfaxSfax3029Tunisia Digital Research Center of Sfax Technopark of SfaxSfax3029Tunisia Department of Information Technology Institute of Graduate Studies and ResearchAlexandria UniversityAlexandriaEgypt

The act of transmitting photos via the Internet has become a routine and significant *** the security measures to safeguard these images from counterfeiting and modifications is a critical domain that can still be further *** study presents a system that employs a range of approaches and algorithms to ensure the security of transmitted venous *** main goal of this work is to create a very effective system for compressing individual biometrics in order to improve the overall accuracy and security of digital photographs by means of image *** paper introduces a content-based image authentication mechanism that is suitable for usage across an untrusted network and resistant to data loss during *** employing scale attributes and a key-dependent parametric Long Short-Term Memory(LSTM),it is feasible to improve the resilience of digital signatures against image deterioration and strengthen their security against malicious ***,the successful implementation of transmitting biometric data in a compressed format over a wireless network has been *** applications involving the transmission and sharing of images across a *** suggested technique utilizes the scalability of a structural digital signature to attain a satisfactory equilibrium between security and picture *** effective adaptive compression strategy was created to lengthen the overall lifetime of the network by sharing the processing of *** scheme ensures a large reduction in computational and energy requirements while minimizing image quality *** approach employs multi-scale characteristics to improve the resistance of signatures against image *** proposed system attained a Gaussian noise value of 98%and a rotation accuracy surpassing 99%.

关键词： Image transmission image compression text hiding Bezier curve Histogram of Oriented Gradients(HOG) LSTM image enhancement Gaussian noise rotation

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Unveiling the Nexus the link between water quality index and phthalic acid ester concentrations in Tigris River

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Emerging Contaminants 2024年第1期10卷 108-116页

作者： Shaymaa Arif Shukur Fikrat M.Hassan saad Sabah Fakhry Department of Biology College of Science for WomenUniversity of BaghdadIraq Ministry of Science and Technology/Food Contamination Research Center Iraq

Monitoring water quality in an aquatic system is a crucial objective in pursuing sustainable development,despite the challenges posed by water scarcity and the impact of climate *** objective of the present study was to examine the water quality of the Tigris River along its course from the point of entry into Baghdad city to its exit,particularly in the presence of Phthalic Acid Esters(PAEs).In both dry and wet seasons and from five sites of the river,nine environmental factors(Turbidity,Total dissolved solids,pH,dissolved oxygen,Chloride,nitrate,phosphate,sulfate,and Biological oxygen demand)and three PAEs(di-n-butyl phthalate(DBP),di-isononyl phthalate(DiNP),and di-2-ethyl hexyl phthalate(DEHP))were *** revealed that the river has high levels of turbidity and Total dissolved solids,slightly alkaline,and well-aerated(dissolved oxygen>7 mg/L).Chloride,nitrate,phosphate,and sulfate values varied between seasons,as high values were recorded in the wet *** oxygen demand levels were higher during the dry *** highest value of total PAEs(2.20μg/L)was observed during the dry season,and the lowest value was in the wet season(1.16μg/L).The HQ results revealed that the river was ranked as a moderate risk *** the water quality index and environmental risk index classified the water quality as a poor category,with a high-risk score in the study sites within Baghdad ***,despite the acceptable limit of PAEs in the river's water(<3μg/L),its presence is an early alarm to withdraw attention to its environmental *** findings highlight the need for appropriate measures to control and remove pollutants to ensure a safe and healthy water supply in the Tigris River.

关键词： Phthalic acid esters di-n-butyl phthalate(DBP) di-isononyl phthalate(DiNP) di-2-ethyl hexyl phthalate(DEHP) Environmental risk index Tigris River Water pollution

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Network Security Enhanced with Deep Neural Network-Based Intrusion Detection System

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Computers, Materials & Continua 2024年第7期80卷 1457-1490页

作者： Fatma S.Alrayes Mohammed Zakariah Syed Umar Amin Zafar Iqbal Khan Jehad saad Alqurni Information Systems Department College of Computer and Information SciencesPrincess Nourah bint Abdulrahman UniversityRiyadh11671Saudi Arabia College of Computer and Information Sciences King Saud UniversityRiyadh11362Saudi Arabia College of Computer and Information Sciences Prince Sultan UniversityRiyadh11586Saudi Arabia Department of Educational Technologies College of EducationImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia

This study describes improving network security by implementing and assessing an intrusion detection system(IDS)based on deep neural networks(DNNs).The paper investigates contemporary technical ways for enhancing intrusion detection performance,given the vital relevance of safeguarding computer networks against harmful *** DNN-based IDS is trained and validated by the model using the NSL-KDD dataset,a popular benchmark for IDS *** model performs well in both the training and validation stages,with 91.30%training accuracy and 94.38%validation ***,the model shows good learning and generalization capabilities with minor losses of 0.22 in training and 0.1553 in ***,for both macro and micro averages across class 0(normal)and class 1(anomalous)data,the study evaluates the model using a variety of assessment measures,such as accuracy scores,precision,recall,and F1 *** macro-average recall is 0.9422,the macro-average precision is 0.9482,and the accuracy scores are ***,macro-averaged F1 scores of 0.9245 for class 1 and 0.9434 for class 0 demonstrate the model’s ability to precisely identify anomalies *** research also highlights how real-time threat monitoring and enhanced resistance against new online attacks may be achieved byDNN-based intrusion detection systems,which can significantly improve network *** study underscores the critical function ofDNN-based IDS in contemporary cybersecurity procedures by setting the foundation for further developments in this *** research aims to enhance intrusion detection systems by examining cooperative learning techniques and integrating up-to-date threat knowledge.

关键词： Machine-Learning Deep-Learning intrusion detection system security privacy deep neural network NSL-KDD Dataset

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Contemporary Study for Detection of COVID-19 Using Machine Learning with Explainable AI

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Computers, Materials & Continua 2024年第7期80卷 1075-1104页

作者： saad Akbar Humera Azam Sulaiman Sulmi Almutairi Omar Alqahtani Habib Shah Aliya Aleryani Department of Computer Science University of KarachiKarachi75270Pakistan Department of Computing Faculty of Engineering Science and TechnologyHamdard UniversityKarachi75540Pakistan Department of Health Informatics College of Applied Medical SciencesQassim UniversityQassim51452Saudi Arabia Department of Computer Science College of Computer ScienceKing Khalid UniversityAbha61421Saudi Arabia

The prompt spread of COVID-19 has emphasized the necessity for effective and precise diagnostic *** this article,a hybrid approach in terms of datasets as well as the methodology by utilizing a previously unexplored dataset obtained from a private hospital for detecting COVID-19,pneumonia,and normal conditions in chest X-ray images(CXIs)is proposed coupled with Explainable Artificial Intelligence(XAI).Our study leverages less preprocessing with pre-trained cutting-edge models like InceptionV3,VGG16,and VGG19 that excel in the task of feature *** methodology is further enhanced by the inclusion of the t-SNE(t-Distributed Stochastic Neighbor Embedding)technique for visualizing the extracted image features and Contrast Limited Adaptive Histogram Equalization(CLAHE)to improve images before extraction of ***,an AttentionMechanism is utilized,which helps clarify how the modelmakes decisions,which builds trust in artificial intelligence(AI)*** evaluate the effectiveness of the proposed approach,both benchmark datasets and a private dataset obtained with permissions from Jinnah PostgraduateMedical Center(JPMC)in Karachi,Pakistan,are *** 12 experiments,VGG19 showcased remarkable performance in the hybrid dataset approach,achieving 100%accuracy in COVID-19 *** classification and 97%in distinguishing normal ***,across all classes,the approach achieved 98%accuracy,demonstrating its efficiency in detecting COVID-19 and differentiating it fromother chest disorders(Pneumonia and healthy)while also providing insights into the decision-making process of the models.

关键词： COVID-19 detection deep neural networks support vector machine CXIs InceptionV3 VGG16 VGG19 t-SNE embedding CLAHE attention mechanism XAI

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A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds

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Synthetic and Systems Biotechnology 2024年第2期9卷 196-208页

作者： Mohamed H.Kalaba Gamal M.El-Sherbiny Osama M.Darwesh saad A.Moghannem Botany and Microbiology Department Faculty of Science(Boys)Al-Azhar UniversityCairo 11884Egypt Agricultural Microbiology Department National Research CentreDokkiCairoEgypt

The goal of this study was to use statistical optimization to change the nutritional and environmental conditions so that Streptomyces baarensis MH-133 could make more active *** trials were used to screen for critical variables influencing productivity using the Placket-Burman Design *** MH-133 is significantly influenced by elicitation,yeast extract,inoculum size,and incubation period in terms of antibacterial activity.A total of 27 experimental trials with various combinations of these factors were used to carry out the response surface technique using the Box-Behnken *** analyses revealed that the model was highly significant(p<0.001),with a lack-of-fit of 0.212 and a coefficient determination(R2)of ***,the model predicted that the response as inhibition zone diameter would reach a value of 27 *** optimal conditions,*** MH-133 produced 18.0 g of crude extract to each 35L and was purified with column *** active fraction exhibiting antibacterial activity was characterized using spectroscopic *** MIC and MBC values varied between 37.5 and 300μg/ml and 75 and 300μg/ml,*** conclusion,the biostatistical optimization of the active fraction critical variables,including environmental and nutritional conditions,enhances the production of bioactive molecules by Streptomyces species.

关键词： S.baarensis MH-133 Placket-Burman designBox-Behnken design ESKAPE Antibacterial activity

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Impact of carbimazole combined with vitamin E on testicular injury induced by experimental hyperthyroidism in adult albino rats:oxidative/inflammatory/apoptotic pathways

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Asian Journal of Andrology 2024年第4期26卷 396-401页

作者： Ramadan S Hussein Moustafa M Eyada Rashad M Mostafa Samah M Elaidy Shereen H Elsayed Hany M saad Department of Dermatology College of Medicine Prince Sattam Bin Abdulaziz UniversityAl-Kharj 16242Saudi Arabia Department of Dermatology Andrology and STDsAssiut Police HospitalAssiut 71525Egypt Department of Dermatology Venereology and AndrologyFaculty of MedicineSuez Canal UniversityIsmailia 41522Egypt Department of Andrology and Sexology Faculty of MedicineSuez Canal UniversityIsmailia 41511Egypt Department of Clinical Pharmacology Faculty of MedicineSuez Canal UniversityIsmailia 41511Egypt Department of Rehabilitation Sciences Faculty of Health and Rehabilitation SciencesPrincess Nourah bint Abdulrahman UniversityRiyadh 12271Saudi Arabia.

Thyroid hormones play essential roles in spermatogenesis,but their effects on infertile males remain poorly *** study aimed to evaluate the impact of combining carbimazole(CBZ)with vitamin E(VE)on testicular injury induced by experimental hyperthyroidism in adult albino rats,focusing on oxidative,inflammatory,and apoptotic *** this experimental study,64 adult male albino Wistar rats were divided into eight groups:Group I(control-untreated),Group II(CBZ-control),Group III(VE-control),Group IV(CBZ+VE-control),Group V(levothyroxine-induced testicular injury),Group VI(levothyroxine+CBZ-treated),Group VII(levothyroxine+VE-treated),and Group VIII(levothyroxine+CBZ+VE-treated).The study was conducted in the Faculty of Medicine,Suez Canal University(Ismailia,Egypt).After cervical decapitation,both testes and epididymis were examined histopathologically and *** differences were observed among groups concerning malondialdehyde(MDA),glutathione(GSH),superoxide dismutase(SOD),and catalase(CAT;all P<0.001).Polymerase chain reaction analysis showed significant differences in tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),Bcl-2-associated X protein(BAX),B-cell lymphoma 2 protein(Bcl2),p53,Caspase-3,Caspase-8,Caspase-9,and nuclear factor-kappa B(NF-κB)mRNA levels(all P<0.001).Hyperthyroid group treated with CBZ alone(Group VI)exhibited testicular side effects,affecting seminiferous tubules and ***,the Group VIII showed improved spermatogenesis and a decrease in testicular side *** addition of VE to the treatment of hyperthyroid rats with CBZ reduced testicular side effects and seminiferous tubular affection when potentially improving *** research is needed to elucidate the underlying mechanisms fully.

关键词： antithyroid drugs testicular injury thyroid gland vitamin E

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Conflicts & Immigration Impacts on Built Environment: A Case Study of Arab Marshes in Iraq

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Journal of Geoscience and Environment Protection 2024年第9期12卷 33-50页

作者： Ola saad Znad University College of Dublin Dublin Ireland

The importance of water to our lives cannot be overemphasized as it is the source of all living things. Water scarcity is a concern in many Middle Eastern nations due to war and politics. Many people lose their essence due to actions made by their rulers, such as the Marsh Arabs. Water and biodiversity should be considered, and governments should be held accountable for attaining a more balanced lifestyle and reducing immigrant levels. Many academics have also explored the hydro-immigration of marshland residents. They suffer more hazards due to a lack of money, poor education, and social instability than their colleagues who remain in their own country. Furthermore, they have insufficient health care and have difficulty integrating into the new community. This implies that not all nations that receive such migrants can implement programs to assist the immigrants. This research reveals how wars, hydro-politics, and immigration issues have drastically affected architecture and social structures in the Arab Marshes region, leading to ecological destruction, mass migrations, and cultural loss. Therefore, this work focuses on the need to critically investigate all factors covered within the context of this research.

关键词： Immigration Hydro Politics Social and Culture Ecology

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CNN Channel Attention Intrusion Detection SystemUsing NSL-KDD Dataset

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Computers, Materials & Continua 2024年第6期79卷 4319-4347页

Intrusion detection systems(IDS)are essential in the field of cybersecurity because they protect networks from a wide range of online *** goal of this research is to meet the urgent need for small-footprint,highly-adaptable Network Intrusion Detection Systems(NIDS)that can identify *** NSL-KDD dataset is used in the study;it is a sizable collection comprising 43 variables with the label’s“attack”and“level.”It proposes a novel approach to intrusion detection based on the combination of channel attention and convolutional neural networks(CNN).Furthermore,this dataset makes it easier to conduct a thorough assessment of the suggested intrusion detection ***,maintaining operating efficiency while improving detection accuracy is the primary goal of this ***,typical NIDS examines both risky and typical behavior using a variety of *** the NSL-KDD dataset,our CNN-based approach achieves an astounding 99.728%accuracy rate when paired with channel *** to previous approaches such as ensemble learning,CNN,RBM(Boltzmann machine),ANN,hybrid auto-encoders with CNN,MCNN,and ANN,and adaptive algorithms,our solution significantly improves intrusion detection ***,the results highlight the effectiveness of our suggested method in improving intrusion detection precision,signifying a noteworthy advancement in this *** efforts will focus on strengthening and expanding our approach in order to counteract growing cyberthreats and adjust to changing network circumstances.

关键词： Intrusion detection system(IDS) NSL-KDD dataset deep-learning machine-learning CNN channel Attention network security

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Refinement Mechanism of Microstructure of Undercooled Nickel Based Alloys

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Journal of Wuhan University of Technology(Materials Science) 2024年第4期39卷 1041-1047页

作者：杜文华 HOU Kai XU Xuguang Ismal saad Willey Liew Yun Hsien AN Hongen Nancy Julius Siambun Bih-Lii Chuab 王洪福 College of Mechanical Engineering North University of ChinaTaiyuan 030051China Faculty of Engineering University Malaysia SabahKota Kinabalu 88400Malaysia

Through the use of purification and recirculation superheating techniques on molten glass,the Ni65Cu33Co2 alloy was successfully undercooled to a maximum temperature of 292 ***-speed photography was employed to capture the process of interface migration of the alloy liquid,allowing for an analysis of the relationship between the morphological characteristics of the alloy liquid solidification front and the degree of ***,the microstructure of the alloy was examined using metallographic microscopy,leading to a systematic study of the microscopic morphological characteristics and evolution laws of the refined structure during rapid *** research reveals that the grain refining mechanism of the Ni-Cu-Co ternary alloy is consistent with that of the binary alloy(Ni-Cu).Specifically,under low undercooling conditions,intense dendritic remelting was found to cause grain refinement,while under high undercooling conditions,recrystallization driven by accumulated stress and plastic strain resulting from the interaction between the liquid flow and the primary dendrites caused by rapid solidification was identified as the main factor contributing to grain ***,the study highlights the significant role of the Co element in influencing the solidification rate and reheat effect of the *** addition of Co was also found to facilitate the formation of non-segregated solidification structure,indicating its importance in the overall solidification process.

关键词： grain refinement recalescence recrystallization undercooling

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