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Is osteoporosis a peculiar association with primary biliary cirrhosis?

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World Journal of Gastroenterology 2005年第34期11卷 5347-5350页

作者： Annarosa Floreani Andrea mega.Valentina Camozzi Vincenzo Baldo Mario Plebani Patrizia Burra Giovanni Luisetto Department of Surgical and Gastroenterological Sciences University of Padova Italy Department of Medical and Surgical Sciences University of Padova Italy Department of Public Health University of Padova Italy Department of Clinical Chemistry University of Padova Italy

AIM：（1） to compare the prevalence of osteoporosis （t-score ≤-2.5 SD） between stage IV PBC patients, and two groups of age- and sex-matched controls： one with hepatitis C virus （HCV）-related cirrhosis, and the other one consisting of a group of healthy subjects from the general population, （2） to identify the main risk factors for the development of bone loss. MEtHODS： thirty-five stage IV PBC patients （mean age 52.5±10 years）, 49 females with HCV-related cirrhosis （mean age 52.9±5.8 years） and 33 healthy females （mean age 51.8±2.22 years） were enrolled in the study. Bone metabolism was evaluated by measuring serum calcium corrected for serum albumin （Ca corr.）, 25-hydroxy vitamin D （25-OH vit D）, parathyroid hormone, osteocaldn. Bone mineral density （BMD） was assessed at the lumbar spine by dual-photon X-ray absorptiometry. RESULtS： Osteoporosis was present in 5/35 PBC patients （14.2%） and in 7/49 HCV-related drrhotic patients （14.3%）, without any statistical difference between the two groups. Among healthy control subjects, none had osteoporosis. No difference was found between the three groups in serum parameters of bone metabolism. Univariate analysis showed that menopausal state and low BMI were significantly correlated with osteoporosis. Multivariate regression analysis showed that menopausal status, BMI〈23, and old age were independent variables significantly correlated with osteoporosis. CONCLUSION： PBC in itself has no negative influence on BMD. End-stage liver disease patients carry a disease-specific risk for osteoporosis, but have an effective risk of bone loss in relation to individual potential risk for each patient. A practical message should be taken into account, that is, every effort should be made to prevent osteoporosis when a patient has simple osteopenia, or if it is a woman in or near menopausal age.

关键词： Osteoporosis Primary biliary cirrhosis HCV infection

Molecular phylogeny of the genus Clibanarius(Decapoda: Anomura: Diogenidae) based on mitochondrial and nuclear DNA sequences

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Journal of Oceanology and Limnology 2019年第5期37卷 1686-1697页

作者： LI Wenjie CHENG Jiao HUI Min sha Zhongli Institute of Oceanology Chinese Academy of SciencesQingdao 266071China Center for Ocean mega.Science Chinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China University of Chinese Academy of Sciences Beijing 100049China

Clibanarius , a genus of hermit crabs, is one of the most diverse genera within Diogenidae. However, studies on the phylogeny within Clibanarius is very limited. We reconstruct the molecular phylogeny of the genus Clibanarius based on two mitochondrial (16S rRNA and COI) and two nuclear protein-coding (NaK and PEPCK) genes using a multigene phylogenetic approach. Eleven selected Clibanarius species from the Indo-West Pacifi c are analyzed and the Bayesian and maximum likelihood analyses produced identical results in topology. Results suggest that C . rutilus and C . infraspinatus are more closely related to each other than to C . snelliusi , and C . striolatus shows a close relationship to C . longitarsus . the close association of C . merguiensis with C . englaucus and C . humilis is strongly supported by the divergence of C . virescens from them. Our phylogenetic results contradict the morphological classifi cation scheme proposed for Clibanarius and indicate that the relative length of dactyls and propodi is not phylogenetically signifi cant in Clibanarius . In addition, we speculate that the morphological characteristic of the median cleft of the telson might be phylogenetically important for Clibanarius .

关键词： Clibanarius phylogeny molecular evolution

2021年~2022年我国猪盖塔病毒流行情况调查及其E2基因序列遗传变异分析

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中国预防兽医学报 2024年第5期46卷 461-467页

作者：吴语晗董雅琴崔进张慧顾丛丛刘爽房琳琳沙洲郑辉尼博魏荣段纲张锋云南农业大学动物医学院云南昆明650201 中国动物卫生与流行病学中心山东青岛266032 桓台县畜牧渔业服务中心山东淄博256400

为了解2021年~2022年我国猪盖塔病毒(GEtV)的流行现状和遗传变异情况,本研究对我国16个省份采集2512份临床样品,采用荧光定量Rt-PCR方法进行GEtV的检测,按照省份和地区进行GEtV流行病学调查分析;采用Rt-PCR方法对部分GEtV阳性样品经PCR... 详细信息

为了解2021年~2022年我国猪盖塔病毒(GEtV)的流行现状和遗传变异情况,本研究对我国16个省份采集2512份临床样品,采用荧光定量Rt-PCR方法进行GEtV的检测,按照省份和地区进行GEtV流行病学调查分析;采用Rt-PCR方法对部分GEtV阳性样品经PCR扩增E2基因并连接至p MD18-t载体并测序,利用DNAStar软件分析E2基因及其编码氨基酸序列与GEtV参考株相应序列的同源性及其氨基酸变异情况;利用mega..0软件构建E2基因的进化树,分析GEtV的基因型及遗传进化关系。结果显示,7个省份猪群样品中均检测出GEtV阳性样品,阳性率为3.34%(84/2512)。其中,陕西省和黑龙江省为首次在猪中检测到GEtV。不同生长阶段猪中,仔猪GEtV的检出率最高,达9.52%。同源性分析显示,获得的32个猪源GEtV E2基因序列之间的同源性为98.3%~100%,与马来西亚GEtV MM2021株相应基因序列的同源性为94.4%~94.9%。氨基酸序列变异分析显示,与参考株相比,本研究测序的E2蛋白有9个氨基酸位点只出现于猪源GEtV。遗传演化分析显示,32个GEtV株均属于III亚群,与III亚群内早期中国M1株、韩国QIAG9301株、日本Kochi等病毒株相比,本研究鉴定的GEtV形成了新的进化分支。本研究揭示了我国当前猪群中GEtV的感染现状及流行株的遗传变异情况,为该病毒的分子生物学研究和相关疫病防控策略的制定提供基础数据和参考依据。

关键词：盖塔病毒 E2基因流行病学调查氨基酸变异遗传进化分析

HSV-1 H129-Derived Anterograde Neural Circuit tracers:Improvements, Production, and Applications

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Neuroscience Bulletin 2021年第5期37卷 701-719页

作者： Hong Yang Feng Xiong Yi-Ge Song Hai-Fei Jiang Hai-Bin Qin Jing Zhou sha Lu Steven FGrieco Xiangmin Xu Wen-Bo Zeng Fei Zhao Min-Hua Luo State Key Laboratory of Virology CAS Center for Excellence in Brain Science and Intelligence TechnologyCenter for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China shanghai Genechem Co.Ltd.shanghai 201203 China Department of Anatomy and Neurobiology School of MedicineUniversity of CaliforniaIrvineCA 92697USA School of Basic Medical Sciences Capital Medical UniversityBeijing 100069China Chinese Institute for Brain Research Beijing 102206China shanghai Public Health Clinical Center Fudan UniversityShanghai 200032China

Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. they have been developed from herpes simplex virus 1(HSV-1) strain H129(H129), and have been successfully applied to map diverse neural ***, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dtK-tdt, which was highly successful, yet improvements are needed. Now, by inserting another tdtomato expression cassette into the H129-dtK-tdt genome, we have created H129-dtK-t2, an updated version of H129-dtK-tdt that has improved labeling intensity. to help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.

关键词： HSV-1 strain H129(H129) Anterograde transneuronal tracer Polysynaptic tracer Monosynaptic tracer Production Application Neural circuit Neural circuit tracing

Polymorphisms and resistance mutations of hepatitis C virus on sequences in the European hepatitis C virus database

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World Journal of Gastroenterology 2016年第40期22卷 8910-8917页

作者： Dimas Alexandre Kliemann Cristiane Valle tovo Ana Beatriz Gorini da Veiga Angelo Alves de Mattos Charles Wood Graduate Program in Medicine: Hepatology-Universidade Federal de Ciências da Saúde de Porto Alegre Porto Alegre RS 90050-170 Brazil Hospital Nossa Senhora da Conceicāo Portto Alegre RS 90050-170 Brazil Department of Basic Health Sciences Laboratory of Molecular Biology Graduate Program in Pathology-Universidade Federal de Ciências da Saúde de Porto Alegre RS 90050-170 Brazil School of Biological Sciences Nebraska Center for Virology University of Nebraska Lincoln NE 68511 United States

AIM to evaluate the occurrence of resistant mutations in treatment-na?ve hepatitis C virus(HCV) sequences deposited in the European hepatitis C virus database(euH CVdb). MEtHODS the sequences were downloaded from the eu HCVdb(https://***/eu HCVdb/). the search was performed for full-length NS3 protease, NS5 A and NS5 B polymerase sequences of HCV, separated by genotypes 1a, 1b, 2a, 2b and 3a, and resulted in 798 NS3, 708 NS5 A and 535 NS5 B sequences from HCV genotypes1a, 1b, 2a, 2b and 3a, after the exclusion of sequences containing errors and/or gaps or incomplete sequences, and sequences from patients previously treated with direct antiviral agents(daA). the sequence alignment was performed with mega.6.06 MAC and the resulting protein sequences were then analyzed using the BioE dit 7.2.5. for mutations associated with resistance. Only positions that have been described as being associated with failure in treatment in in vivo studies, and/or as conferring a more than 2-fold change in replication in comparison to the wildtype reference strain in in vitro phenotypic assays were included in the *** the Q80 K variant in the NS3 gene was the most prevalent mutation, being found in 44.66% of subtype 1a and 0.25% of subtype 1b. Other frequent mutations observed in more than 2% of the NS3 sequences were: I170V(3.21%) in genotype 1a, and Y56F(15.93%), V132I(23.28%) and I170V(65.20%) in genotype 1b. For the NS5 A, 2.21% of the genotype 1a sequences have the P58 S mutation, 5.95% of genotype 1b sequences have the R30 Q mutation, 15.79% of subtypes 2a sequences have the Q30 R mutation, 23.08% of subtype 2b sequences have a L31 M mutation, and in subtype 3a sequences, 23.08% have the M31 L resistant variants. For the NS5 B, the V321 L RAV was identified in 0.60% of genotype 1a and in 0.32% of genotype 1b sequences, and the N142 t variant was observed in 0.32% of subtype 1b sequences. the C316 Y, S556 G, D559 N RAV were identified in 0.33%, 7.82% and 0.

关键词： Hepatitis C virus resistance Quasispecies Direct antiviral agents Polymorphisms Drug resistance

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世界核心医学期刊文摘（神经病学分册） 2005年第9期1卷 16-17页

作者： mega.M.S. Dinov I.D. Porter V. 高中宝 Alzheimer's Disease Center and Center on Aging Providence Health System 10150 SE 32nd Ave. Portland OR 97222 United States Dr.

Background: Regional brain correlates of treatment with cholinesterase inhibit ors in those with Alzheimer disease are unknown. Objective: to map brain metabol ism associated with the treatment response to galantamine with fludeoxyglucose F 18 positron emission tomography in patients with Alzheimer disease. Design: thi s is a hypothesis-driven, prospective, open-label study of 19 patients with mi ld to moderate Alzheimer disease examined before and after treatment with the ch olinesterase inhibitor galantamine. Clinical examinations included the cognitive portion of the Alzheimer Disease Assessment Scale, the Mini-Mental State Exami nation, and the Neuropsychiatric Inventory. Imaging was performed using fludeoxy glucose F 18 positron emission tomography. the positron emission tomographic dat a, registered to a probabilistic anatomical atlas, were subjected to a voxel-ba sed analysis of 3 subgroups: total patient analysis, cognitive analysis, and beh avioral analysis. Subvolume thresholding corrected random lobar noise to produce 3-dimensional significance maps. Results: the total group analysis showed an i ncrease in left caudate metabolism with no significant change in clinical outcom es for the total group with treatment. Subgroup analysis of cognitive and behavi oral responders demonstrated a significant activation of a striatal-thalamofr- ontal network with galantamine treatment that was not present in patients whos e condition worsened or was unchanged by therapy. In cognitive subgroups, change in left anterior cingulate metabolism significantly correlated with change in t he cognitive portion of the Alzheimer Disease Assessment Scale (r=0.70, P=.02); in behavioral subgroups, right cingulate metabolic change significantly correl ated with improve ment in depression and right ventral putamen metabolic change with improvement i n apathy (r=0.63, P th). Conclusion: Cognitive and behavioral respon ders to galantamine therapy show clinically related impr

关键词：脱氧葡萄糖 PEt 临床反应加兰他敏认知功能胆碱酯酶抑制剂阿尔茨海默病亚组神经心理扣带回

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三菱重工地面燃气轮机热障涂层研究进展

热喷涂技术 2014年第3期6卷 56-62页

作者： Yoshifumi Okajima daisuke Kudo Naotoshi Okaya taiji torigoe Hideaki Kaneko Masahiko mega.Eisaku Ito Junichiro Masada Keizo tsukagoshi 吴琼

燃气轮机联合循环热效率的提高,要求涡轮进口温度不断增加。目前,三菱重工(MHI)已经积极参与日本国家项目,通过发展1700℃级燃气轮机,以使联合循环热效率实现达到62%指标。在此国家项目中开发的部分组件技术适用于1600℃级燃气轮机M501J... 详细信息

燃气轮机联合循环热效率的提高,要求涡轮进口温度不断增加。目前,三菱重工(MHI)已经积极参与日本国家项目,通过发展1700℃级燃气轮机,以使联合循环热效率实现达到62%指标。在此国家项目中开发的部分组件技术适用于1600℃级燃气轮机M501J,它具有世界上最高的涡轮进口温度。而热障涂层(tBC)是实现此目标的最关键技术之一。在发展先进的热障涂层中,在MHI工厂中建立名为t点的试验电厂是有必要的。在真实热部件中制备的先进tBC可以在工厂中进行验证,并且运行后的效果可以得到反馈。本文讨论了tBC的开发以及在三菱重工实际电厂中的验证。

关键词：燃气轮机热障涂层电厂

Bioactive substances of various seaweeds and their applications and utilization

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Journal of Oceanology and Limnology 2019年第3期37卷 779-782页

作者： DUAN Delin CRItCHLEY Alan t. FU Xiaoting PEREIRA Leonel Key Lab of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Center for Ocean mega.Science Chinese Academy of ScienceQingdao 266071China State Key Lab of Seaweed Bioactive Substances Qingdao 266400China Verschuren Centre for Sustainability in Energy and the Environment Cape Breton UniversitySydneyNova Scotia B1P 6L2Canada College of Food Science & Engineering Ocean University of ChinaQingdao 266000China MARE (Marine and Environmental Sciences Centre) Department of Life SciencesFaculty of Sciences and TechnologyUniversity of CoimbraCoimbra 3000-456Portugal

Over the past century or more,traditional uses of seaweeds began and initially comprised the simple collection,sorting and drying of seaweeds harvested from the wild,or cast upon the beach,for use as soil“manure/fertilizer/improver”.Some macroalgae were used for unprocessed food purposes and subsequently harvested and cultivated biomasses of selected brown and red seaweeds becameindustrially processed to yield alginates,mannitol,iodine,agar,agarose,carrageenan,and more recently biostimulants,*** from various seaweeds are very diverse and are commonly applied in a range that includes textiles and dying,food/feed and supplements and specialized medical applications.

关键词： century collection macroalgae

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10个菲律宾蛤仔野生群体的遗传多样性研究

海洋与湖沼 2016年第3期47卷 549-556页

作者：胡利莎张振马培振王海艳中国科学院海洋研究所青岛266071 中国科学院大学北京100049 中国海洋大学青岛266003

对我国沿海地区10个菲律宾蛤仔野生群体线粒体16S r RNA和COI基因部分序列进行测序,分别得到了长度为473bp和632bp的片段。结果表明,16S r RNA 193条序列A+t平均含量为66.6%,共检测到21个变异位点,193个个体具有22种单倍型;COI基因183... 详细信息

对我国沿海地区10个菲律宾蛤仔野生群体线粒体16S r RNA和COI基因部分序列进行测序,分别得到了长度为473bp和632bp的片段。结果表明,16S r RNA 193条序列A+t平均含量为66.6%,共检测到21个变异位点,193个个体具有22种单倍型;COI基因183条序列A+t平均含量为64.8%,共检测到126个变异位点,183个个体具有67种单倍型。基于群体间遗传距离利用mega..1软件构建10个群体的NJ树,聚类结果表明,大连群体和荣成群体聚为一支,其余8个群体聚为一支。AMOVA分析表明,大连群体和荣成群体间分化不显著,而荣成、大连群体与其余8个群体间的分化达到极显著水平(P<0.01),说明我国沿海的菲律宾蛤仔野生群体存在一定的遗传分化。

关键词：菲律宾蛤仔 COI基因 16SrRNA基因遗传多样性