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Bicornuate Uterus: Infertility Treatment and Pregnancy Continuation without Cerclage: An Unusual Case

Open Journal of Obstetrics and Gynecology 2014年第15期4卷 981-985页

作者： meenal parmar Surabhi Tomar Department of Obstetrics and Gynaecology Nims Medical College and Hospital Jaipur India

Introduction: Uterine malformation in general population is around 7% - 8%. Abnormal fusion of the para-mesonephric duct (mullerian duct) during embryonic life results in a variety of congenital uterine malformations, such as uterus didelphys, uterus bicornis bicollis, uterus bicornis unicollis, uterus subseptae, uterus arcuatus, uterus unicornis. The bicornuate uterus accounts for approximately 10% of the mullerian anomalies. Women with bicornuate uterus have no extra uterine infertility issues. The uterine malformations are known to be associated with spontaneous miscarriages, intrauterine growth restriction, preterm deliveries, preterm prelabour rupture of membranes, breech presentation and increased rate of caesarean delivery. The rates of spontaneous abortion and premature delivery have been reported to reflect the degree of non fusion of the horns. The common complications and adverse reproductive outcomes associated with bicornuate uterus are recurrent pregnancy loss (25%), preterm birth (15% - 25%) and cervical insufficiency (38%). We reported a case of bicornuate unicollis pregnancy which was infertility treated and carried till 38 weeks and had a good outcome. Case Report: A 32-year-old nullipara presented to Infertility OPD at NIMS Medical College and Hospital with the chief complaint of inability to conceive in spite of having regular sexual intercourse for 12 years without using any contraception. Patient was then investigated, bicornuate uterus identified. Infertility treatment was done by Intrauterine Insemination (IUI). Patient conceived and pregnancy continued till term uneventfully. Discussion: The prevalence of uterine anomalies is 7% - 8%. And now because of better availability of diagnostic modalities, better detection of such anomalies is possible. Bicornuate uterus is a congenital uterine anomaly that results from defective lateral fusion of the paramesonephric ducts at about the tenth week of intrauterine life around the fundus. A bicornuate

关键词： Bicornuate Uterus Paramesonephric Mullerian Duct

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Bicornuate Uterus: Infertility Treatment and Pregnancy Continuation without Cerclage:An Unusual Case

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Open Journal of Obstetrics and Gynecology（妇产科期刊） 2014年第15期

作者： meenal parmar Surabhi Tomar

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A versatile method for enumeration and characterization of circulating tumour cells from patients with breast cancer

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Journal of Cancer Metastasis and Treatment 2017年第1期3卷 23-33页

作者： Ujjwala M.Warawdekar Vani parmar Aruna Prabhu Abhay Kulkarni meenal Chaudhari Rajendra A.Badwe CRI Lab Advanced Centre for Treatment Research and Education in Cancer(ACTREC)Tata Memorial CentreNavi Mumbai 410208India Department of Surgery Breast UnitTata Memorial HospitalTata Memorial CentreParelMumbai 400012India Homi Bhabha National Institute Anushakti NagarMumbai 400085India.

Aim:To establish a standardized protocol for the isolation and enumeration of circulating tumor cells(CTCs)from peripheral blood of patients with metastatic breast ***:The protocol used tumor cells spiked in a lymphoid cell line with detection by flow cytometry and quantitative reverse transcription polymerase chain reaction(QRT-PCR).Cells of the human mammary cancer subtypes were spiked into Jurkat cells,which served as the lymphocyte designate in numbers from 10 to 500 per 105 Jurkat *** mixed population was probed for CD45,EpCAM,and pancytokeratin acquired from flow cytometry and characterized by ***-PCR was done for CK-19,MUC-1,EpCAM,and *** was attained with blood samples from 22 patients with metastatic breast cancer and 20 healthy ***:Flow cytometry could detect 1 breast cancer cell per 100,000 Jurkat cells,with similar detection levels in the breast cancer *** from patients with breast cancer showed a range of CTCs from 1-85 per 10 mL of *** of expression for EpCAM,CK-19,Muc-1,and Her2neu confirmed the presence of CTCs in 76%of ***:Density gradient and immunomagnetic enrichment accomplished isolation of CTCs and quantitation was achieved using flow *** QRT-PCR and imaging further validated these findings,rendering a robust methodology.

关键词： Circulating tumor cells cytokeratin-19 flow cytometry circulating tumor cell enumeration quantitative reverse transcription polymerase chain reaction EpCAM

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